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At least 127 records · Page 7Linked to original sources
PubMed1992

Cold-hardiness in Pelecitus fulicaeatrae (Nematoda: Filarioidea), a parasite of the ankles of Fulica americana (Aves).

The filarioid nematode Pelecitus fulicaeatrae (Diesing, 1861) is considered cold-hardy. Adults and microfilariae became motile when placed in saline at 22 C after having been removed from thawed carcasses of their host, the American coot (Fulica americana Gmelin) (Aves: Gruiformes). Adult nematodes from 5 of 12 carcasses became active as did microfilariae from 4 of 5 carcasses. Carcasses had been frozen at an undetermined temperature below 0 C for an initial 14 days and then at -21 to -24 for 100-159 days.

Animals↗
PubMed1989

Identification of a potential protective microfilarial antigen from Litomosoides carinii (Nematoda, Filarioidea).

The proteins of the filarial parasite Litomosoides carinii that are presented to the host in the course of a natural infection were assessed by surface and intrinsic labelling, microfilarial agglutination assays and ELISA. Surface labelling revealed several proteins present on all parasite stages which were recognised by infected animals. Only one major surface protein of 55 KD was found on microfilariae, while in all other stages (adult males, adult females and infective larvae isolated from pool animals) a range of proteins could be labelled. Immunisation with live microfilariae obtained from blood provoked sera which agglutinated microfilariae in vitro and co-precipitated the 55 KD surface protein. A proportion of animals quantitatively infected with 30 infective larvae (L3) developed antibodies during prepatency that were capable of agglutinating microfilariae, suggesting a common epitope between L3/L4 and microfilariae. Intrinsic labelling of microfilarial proteins was used to identify three additional proteins of 16, 29 and 43 KD which may also be important during infection. The anti-microfilarial antibody titre was quantified by ELISA using microfilarial antigen and sera from various time points during the course of a natural infection.

Agglutination Tests↗
PubMed1988

Homologous and crossreacting immune response of the jird and cotton rat against microfilariae of Dipetalonema viteae and Litomosoides carinii (Nematoda: Filarioidea).

Consecutive injections of microfilariae of L. carinii and D. viteae were carried out in the homologous or heterologous sequence into the corresponding natural or into the experimental hosts. Microfilariae of D. viteae are imunogenic in Sigmodon specifically and crossreacting as well. However, in the natural host, Meriones, they are doubtlessly tolerogenic. The sojourn and level of microfilaraemia are prolonged resp. higher when living D. viteae-Mf are injected s.c. followed by an i.v. injection. However, freeze-killed microfilariae of D. viteae are immunogenic in Meriones. Obviously the two filarial worms living in small mammals realize the balance with their natural hosts in quite a different manner. Which one of the two possibilities, or if one of them at all, reflects the relationship in human pathogenic filariae remains open.

Animals↗
PubMed1985

Development of Dirofilaria immitis third stage larvae (Nematoda: Filarioidea) in micropore chambers implanted into surrogate hosts.

Groups of 100 third stage larvae of Dirofilaria immitis recovered from Aedes aegypti were loaded into 250 microliters capacity micropore chambers (0.3 micron pore size) and implanted into the peritoneal cavity of mice, jirds, cotton rats and ferrets. In all hosts 74-87% of larvae moulted by 74 hours, with less than 5% mortality. The fourth stage worms recovered at 74 hours were cultured in vitro in L-15 (Leibovitz) medium plus 20% foetal bovine serum with a dog sarcoma feeder cell line. After 96 hours cultivation, the larvae were comparable in size to those recovered from the dog 7 days post-infection (Orihel 1961). Chambers were also recovered from mice at intervals up to day 8. Larval survival at the longer times was approximately 90% and growth was similar to that reported for larvae in the dog. Incubation of third and fourth stage larvae in chambers with Trypan blue demonstrated that the gut is non-functional until the completion of the third moult.

Animals↗
PubMed1978

Tetrapeltalonema (t.) mystaxi sp. n. (Nematoda: Filarioidea) from Brazilian moustached marmosets, Saguinus m. mystax.

Tetrapetalonema (T.) mystaxi sp. n. is described from the subscapular connective tissues of Saguinus mystax. The parasite is placed in the subgenus Tetrapetalonema and most closely resembles Tetrapetalonema atelensis, from which it can be differentiated by its long esophagus, size and shape of the spicules, and the size and arrangement of nuclei in the tail of the microfilaria.

Animals↗
PubMed1979

A new species of Breinlia (Breinlia) (nematoda: Filarioidea) from the South Indian flying squirrel Petaurista philippensis (Elliot).

Breinlia (Breinlia) petauristi sp. n. from the abdominal cavity of the flying squirrel, Petaurista philippensis (Elliot), from Kerala, India, is described. Of the 15 recognized species of the subgenus, B. petauristi most closely resembles B. trichosuri in the body size, length of spicules, and number of caudal papillae. The detailed structure of the spicules is, however, markedly different in the 2 worms. This is the third species of Breinlia to be reported from a host other than an Australian marsupial.

Animals↗