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[A rare case of human autochthonous dicrocoeliasis in Czechoslovakia].

The first case of not imported dicroceliasis in Czechoslovakia is reported in an eleven-year-old boy. The patient suffered from gastrointestinal complaints for about half a year. Diarrhea frequently alternated with constipation, the abdominal pain was mainly in the region of the pancreas. With the exception of eosinophilia (16%), all other laboratory findings (blood count, ELFO, urinalysis) were within normal values. Eggs of Dicrocoelium dendriticum were repeatedly found in stool specimens. The patient was successfully treated with Bithin (4,6-dichlorpentol). Examination of the other members of the family proved negative. At present the patient is without complaints, stool examinations for parasites were repeatedly negative. Contact with sheep or consumption of contaminated liver was not demonstrated. The parasitic infestation may have occurred via an intermediate host, as the patient was keen on collecting different animals, particularly snails.

Child↗

Comparative study on cancer incidence in neighboring regions of Hungary, Austria, Yugoslavia and Czechoslovakia in 1969-1980. Pannonian Tumor Epidemiology Study Group.

The present collaborative study was performed in four confined, neighboring regions of Austria, Hungary, Yugoslavia and Czechoslovakia with different demographic and occupational characteristics of their respective populations, various structures of medical facilities, and cancer registration systems. Crude and age-standardized incidence rates and trends of six major cancer sites (stomach, colorectum and lung in both sexes as well as breast, uterine cervix and uterine body in women) in three 4-year periods (1969-1972, 1973-1976, 1977-1980) were computed and mutually compared in the mentioned regions. Relatively high, different but more or less decreasing incidence rates of stomach, and varying but mainly increasing rates and trends of colorectal cancer were observed, while lung cancer incidence rates and tendencies showed great variations in both sexes. For women, besides continuous increase, the tendencies towards culmination and stabilization were found in regions with the highest incidence rates of breast and uterine body cancer in comparison with the more or less declining rates of uterine cervix cancer over the period studied. The observed different incidence rates and trends of these and obviously of other cancer sites require further investigations using more sophisticated epidemiological methods and approaches.

Austria↗

[Further development of the transfusion service in Czechoslovakia].

The blood transfusion service in the CSSR must react in its future activities not only to recent advances in the discipline but also to the present adverse position as regards the spread of AIDS and other virus diseases. Therefore the further development in the CSSR is focused above all on the prevention of transmission of virus infections, incl. organized development of autologous transfusions. To this end the training of new specialists in the discipline must be focused, i. e. on training of clinical transfusiologists. As the basis the concept is used that haematology and blood transfusion in Czechoslovakia is a specialization of internal medicine or paediatrics. In the development of laboratory diagnosis attention is focused on the use of micromethods with the possibility of automation of examinations as well as economy of diagnostic procedures and staff.

Blood Transfusion↗

[Cooperation between East Germany and Czechoslovakia in studying the effect of environmental pollution on school children].

This report presents key outcomes of joint growth and development studies conducted in children permanently living in areas adversely affected by pollutants in air. Further attention is focussed on the evaluation of effects of some of the so-called compensatory measures applied in Czechoslovakia, namely a 3-week outdoor schooling organized for these children in the relatively clean recreational areas. The beneficial health effects of year's season and specifically of the spontaneous, active and sufficiently long outdoor exercise are documented in graphs. As it is pointed out in this context, children in the place of their permanent residence tend to have relative little exercise outdoor, may run an increased risk of exposure to indoor pollutants, and may suffer from passive smoking effects if their parents cannot refrain from smoking habits at home.

Air Pollution↗

Entamoeba polecki: morphology, immunology, antigen study and clinic of the first infections in Czechoslovakia.

Entamoeba polecki Prowazek, 1912 was recorded for the first time in Czechoslovakia in two students from Kampuchea. Uninucleated cysts of diameter 14.2-15.7 micron with nuclei diameter 3.2-4.2 micron were found-repeatedly in stool samples taken from them. The nucleus accounted in average for 24.3% of the cyst diameter. The isolation and two following subinoculations on Dobell-Leidlaw medium were achieved, more abundant growth was recorded on medium with pig serum. Sera of both students were positive in serological tests using the E. histolytica antigen. No serious clinical symptoms were observed, both patients were cured successfully by metronidazole and ornidazole. Electroimmunotransfer blots were used to characterize E. polecki as a separate species. The antigenic structure of polyxenically grown E. polecki was compared with the antigenic structure of E. histolytica (axenically grown HK-9 strain and 3 polyxenically grown strains). Different blot patterns of both species were obtained, but common fractions of 30-40 kD probably responsible for serological cross-reactions were found.

Amebiasis↗

Hemorrhagic fever with renal syndrome in Czechoslovakia: detection of antigen in small terrestrial mammals and specific serum antibodies in man.

A direct ELISA technique was used for demonstration of HFRS virus antigen in suspensions of the lungs obtained from 774 wild small mammals belonging to 13 zoological species trapped in 21 localities in Czechoslovakia between 1983 and 1984. Western serotype of HFRS virus was demonstrated in the lungs of 8 Microtus arvalis voles caught in the areas of central Bohemia, southern Moravia and western Slovakia, and in one Clethrionomys glareolus vole from the area of eastern Slovakia. The eastern serotype antigen was found in one Apodemus agrarius field mouse captured in the area of eastern Slovakia. The antigen titres as determined in the lungs of these rodents by the ELISA and RIA techniques were relatively high. Human sera examined for the presence of HFRS-specific antibody by the indirect fluorescence and RIA techniques were 76 serum specimens from chronic kidney patients treated by hemodialysis and 349 sera of adult males over 35 years of age coming from various districts of Bohemia and Moravia. Antibodies against HFRS virus antigen were found in 4 sera obtained from northern Bohemian men. The results provide the evidence which suggests that the natural foci of HFRS are likely to occur across much of the Czechoslovak territory, and confirms the focality of this infection in at least some of the localities characteristic in the 1950s and 1960s of the incidence of severe sporadic cases of HFRS.

Adult↗

Gastrointestinal parasitoses discovered in agricultural workers in South Bohemia, Czechoslovakia.

In the years from 1975 to 1982 1,750 persons, mostly employed by agricultural enterprises in the South Bohemian Region in Czechoslovakia, were examined. We discovered seven species of parasites: Taenia saginata in 0.3%, Enterobius vermicularis in 10.1%, Giardia lamblia in 1.0%, Endolimax nana in 0.8%, Entamoeba coli in 0.7%, Entamoeba hartmanni in 0.2%, and Chilomastix mesnili in 0.5%. The greatest number of parasites was found in students of the Secondary agricultural and technical school. Only two species of parasites were diagnosed in children of the employees. The incidence of E. vermicularis was 75% in children, in adult employees of agricultural enterprises, however, only 9.8%.

Agricultural Workers' Diseases↗

Serological diagnostics of imported malaria in Czechoslovakia.

Two serologic techniques for malaria detection were compared in this study; the indirect fluorescent antibody (IFA) test used in 214 persons (38 Czechoslovak citizens returning from visits to tropical countries and 176 foreign visitors arriving to Czechoslovakia from areas endemic for malaria) and the indirect hemagglutination (IHA) test employed in 125 persons (29 Czechoslovak citizens and 96 foreigners). Comparisons revealed poor correlation between the IFA test and IHA test data. Of the two tests the IFA test appeared to be distinctly more reliable, more sensitive and more specific, the IHA test turned out to yield both false positive and false negative results. The antigen from Plasmodium gallinaceum gave lower IFA titres than P. falciparum antigen, but reacted with antibodies to all species of human plasmodia, and gave reliable test results. Positive serologic responses were appreciably more frequent in foreigners (46.0%) than Czechoslovak citizens (23.7%). The maximum percent positivity for malarial antibody was among individuals from tropical countries of Africa (74.6%), seropositivity in people from malaria endemic areas in Asia and Latin America was far less frequent (28.4% and 44.4%, respectively).

Animals↗

Epidemiology and significance of Q fever in Czechoslovakia.

Over the last 35 years most parts of Czechoslovakia have experienced Q fever. Of particular note were outbreaks in factories processing imported cotton, wool and hides that had been contaminated in their countries of origin. Some areas of the southern part of Central Slovakia became a natural focus of Q fever with the Dermacentor marginatus tick being the main vector of coxiellae. Currently, Coxiella (C.) burnetii infection appears to be dormant in the country since only sporadic cases are encountered. Cattle vaccination practiced since 1980 may have contributed to the decreasing incidence of human Q fever.

Animals↗

Hereditary angioedema in Czechoslovakia. Clinical, immunological, genetic and therapeutic studies of 16 families.

The analysis of the findings in 16 families with hereditary angioedema detected in Czechoslovakia over the years 1975-1986 is being presented. In 14 families C1-inhibitor deficiency and in two families afunction of C1-inhibitor was established. Of the total number of 175 examined family members C1-inhibitor defect was registered in 66 subjects (60 with deficiency and 6 with afunction), and of these 48 suffered from clinical symptoms of edematous attacks affecting the skin, larynx, intestine and urinary tract, whereas 18 subjects were asymptomatic. Genealogical studies confirmed that the defect is inherited as an autosomal dominant trait. In 16 patients long-term prophylactic treatment with Danol was introduced. The effective minimum dosage was tested individually for each patient.

Angioedema↗

First experience with ELISA serosurvey for tick-borne borreliosis (Lyme disease) in Czechoslovakia.

Antibodies to Borrelia burgdorferi were searched for in the sera from two groups of hospital patients and one group of healthy agricultural workers. The antibody response was measured by the authors' own modification of the ELISA method employing the antigen prepared from Borrelia recurrentis strain propagated in laboratory mice. Positive antibody titres to Borrelia were demonstrated in 5 out of the 44 patients admitted to the Infectious Diseases Clinic, in 8 out of the 32 patients from the Neurologic Clinic and in 16 out of the 52 healthy agricultural workers living in areas infested with ticks. These findings are interpreted as showing the topicality of the Lyme disease problem in Czechoslovakia.

Adult↗

Deficiency of C2, the second complement component, in the family of a patient with SLE-like syndrome: the first case of hereditary C2 deficiency in Czechoslovakia.

A family with hereditary C2 deficiency was discovered in Czechoslovakia. The proband is a 47-year-old female with a SLE-like syndrome and zero activity of the classical complement pathway. Functional CH50, C1, C2, and C4 estimations for all family members revealed a homozygous C2 deficiency in both the proband and her elder sister, and several heterozygotic C2-deficient individuals. The defect segregates with haplo-type HLA A25, B18, DR2.

Adult↗

A new host, Simulium argyreatum Meig., for the cytoplasmic polyhedrosis virus of blackflies in Czechoslovakia.

The cytoplasmic polyhedrosis virus (CPV) from the midgut of blackfly larvae is recorded from a population of Simulium argyreatum in Czechoslovakia. A 7.7% infection of 2766 sampled larvae was manifested by white spots in their body. 85% of the white-spotted larvae were infected with CPV when sampled in June. In August 9% of 1377 larvae had white spots but only 10.6% of them were with CPV. Out of 823 collected pupae, only 3 imperfect pupae had the virus in apparent form. Other infections producing white spots in larvae were due to the microsporidia Plistophora multispora, P. simulii, P. debaisieuxi, Thelohania fibrata, the fungus Coelomycidium simulii and the mermithids.

Animals↗

Water snails as paratenic hosts of Hymenolepididae Fuhrmann, 1907 in Czechoslovakia.

The distribution of cysticercoids of Hymenolepididae Fuhrmann, 1907 parasitizing water birds (Anseriformes and Ralliformes) in Czechoslovakia was studied. A total of 2970 snail specimens (429 Lymnaea stagnalis (L.), 531 L. ovata (Drap.), 1, 462 L. peregra (O. F. Müll.), 158 L. auricularia (L.), 262 Planorbis planorbis (L.) and 65 Viviparus viviparus (L.)) were examined for this purpose. Among them, 122 snails were infected with cysticercoids of 13 cestode species (Dicranotaenia coronula (Dujardin, 1845), Diorchis inflata (Rudolphi, 1810), D. nyrocae Yamaguti, 1935, D. ransomi Schultz, 1940, Diploposthe laevis (Bloch, 1782), Echinocotyle rosetteri Blanchard, 1891, Fimbriaria fasciolaris (Pallas, 1781), Microsomacanthus compressa (Linton 1892), M. paracompressa (Czaplinski, 1956), M. paramicrosoma (Gasowska, 1931), M. spiralibursata (Czaplinski, 1956), Sobolevicanthus gracilis (Zeder, 1803), and S. octacantha (Krabbe, 1869).

Animals↗

[The first occurrence of demodicosis in cattle in Czechoslovakia].

Demodectic lesions were found in 37 per cent out of the total number of seventy-five cows examined by palpation in a large cow-house in South Bohemia, Czechoslovakia. These lesions were intradermal nodules, mostly 2 to 6 mm in diameter, located in the corium in the anterior parts of the body. The animals had 1 to 75 lesions (on the average 11.6). Their age was three to seven years. Their overall health condition was good and their behaviour was not affected. Microscopic examination revealed large numbers of Demodex bovis Stiles, 1892, causative agent of demodicosis, in the contents of nodules in five out of six examined animals. Histopathological changes in two nodules were characterized by inflammatory granulomatous structures containing large numbers of mites, epithelioid cells with rich, pale cytoplasm, giant cells of the foreign body type, and inflammatory infiltrates consisting mostly of lymphocytes. Fibroproduction in the contents of granulomas and along their periphery was markedly low.

Animals↗

Identity of Argas (Argas) polonicus populations in Czechoslovakia and Poland.

The author made a morphological and biological comparison between the population of Argas (A.) polonicus Siuda, Hoogstraal, Clifford et Wassef, 1979 from Poland and the population of that species from Czechoslovakia, denoted in previous author's papers as Argas (A.) vulgaris Filippova, 1961. The comparison of morphometric features as well as the results obtained in the studies of those argasids in SEM have demonstrated some statistically significant differences which the author evaluates as intraspecific adaptive variability and does not attach any taxonomic importance to them. Experimental hybridization of P1 and F1 generations in both populations has not suggested any genetic incompatibility. A few differences in the developmental cycle of homogamic crosses in the Polish tick population is likely to be the consequence of the adaptation of this population to different living conditions. Due to the lack of taxonomically valuable differences the author includes the members of the Czechoslovak population of these argasids in the species A. polonicus.

Animals↗

[Dispensary care of persons suffering from non-tuberculous respiratory diseases in czechoslovakia (author's transl)].

In Czechoslovakia phtisiology was enlarged into the specialization of tuberculosis and respiratory diseases by a decree of the Ministry of Health in 1966. The dispensary care of pneumophtisiological institutions aims--besides tuberculosis control--especially at the following groups of patients suffering from non-tuberculous respiratory diseases: 1. cases having tendency to deterioration or with a complicated course, 2. cases with functional disorders of the respiratory system, 3. cases in conditions which may influence unfavourably the respiratory disease. When patients with non-tuberculous respiratory diseases are in the care of other physicians, the pneumophtisiologists render necessary consultations and decide which patients will be taken over into the dispensary care of pneumophtisiological department.

Ambulatory Care↗