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Differentiation of nuclei during keratinization in middle ear cholesteatoma. DNA cytophotometry completed by computerized image analysis.

Quantitative DNA cytophotometric techniques were applied to judge the alteration (differentiation) and ultimate fate of nuclei during keratinization in human middle ear cholesteatoma. Compared with a healthy epidermis, a tendency towards postponed nuclear degradation was noticed. Two patterns governing the loss of DNA are recognized. In one group, the mean nuclear DNA content declines continuously, starting in the nearest suprabasal layers and continuing throughout the prickle and granular cell stages, where the ultimate degeneration of nuclei takes place. This pathway corresponds to that observed in epidermis, but evolves more slowly. In another group of samples, the onset of the DNA decline is delayed to the upper prickle cells, exceptionally to more terminal stages of keratinization. During matrix keratinization, a profound nuclear remodelling takes place, similar to that in epidermal tissues, as far as eu- and heterchromatin DNA and area data are concerned. However, euchromatinization of nuclei in matrix prickle cells is more pronounced than in epidermal tissues. The topography of residual heterochromatic clumps does not reflect a persistent margination as in epidermal nuclei, but is the result of more individualized rearrangements. The changes in karyotype are less elaborate when the complete decline of the nuclear DNA content only occurs during terminal keratinization.

Cell Nucleus↗
PubMed1990

[DNA content and its distribution pattern of poorly differentiated nasopharyngeal carcinoma measured by Feulgen cytophotometry].

Nuclear area and DNA content of the normal columnar cells and cancer cells which were small, fusiform, irregular, vesicular-nuclear, giant malformed-nuclear and giant vesicular-nuclear were measured by Leitz MPV-III microscope photometer with HP-85 microcomputer in 32 poorly differentiated nasopharyngeal carcinoma (NPC) and 5 chronic nasopharyngitis sections. Based on different DNA contents and distribution patterns, it was divided into 4 cancer cell populations: small, fusiform and/or irregular, vesicular-nuclear and giant tumor cell populations. It should be noted that the nuclear DNA pattern of vesicular-nuclear cancer cell population had pathognomonic characteristics. The nuclear unit DNA content ratio of vesicular-nuclear cancer cells to lymphocytes was under 0.4. This cancer cell population was sensitive to irradiation. If the biological characteristics of these four cancer cell populations could be clarified not only in nuclear DNA pattern but also in biochemistry at molecular level, it will be possible to design the different treatment trials on the different cancer cell populations, then resulting in better prognosis of NPC patients.

Carcinoma, Squamous Cell↗