Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “CHLOROGENIC ACID”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 127 records · Page 7Linked to original sources
PubMed1991

[Application of differential derivative spectrophotometry to the determination of total chlorogenic acid in Lonicera japonica Thunb. and yinqiao jiedu pian].

The total chlorogenic acid in Lonicera japonica and yinqiao jiedu pian was determined by differential derivative spectrophotometry in this paper. Quantitative analysis of this component in eight mixtures of medicinal herbs can be done without any separation. The average recovery of total chlorogenic acid is 100.40 +/- 1.82% (CV). The method features good reproducibility, favorable stability and high sensitivity.

Chlorogenic Acid↗
PubMed2005

Hypoglycemic effect and chlorogenic acid content in two Cecropia species.

The hypoglycemic effect of methanol leaf extracts from Cecropia obtusifolia and C. peltata was evaluated in healthy mice. A significant decrease (p < 0.05) in plasma glucose levels was recorded 2 and 4 h after a single oral administration of methanol extracts (1 g/kg). This effect was correlated with the chlorogenic acid contents in both species; C. peltata, containing 19.84 +/- 1.64 mg of chlorogenic acid/g of dried leaves produced the highest decrease (D(alpha 2,60) = 20.18, p < 0.05) of plasma glucose levels (52.8%). The extracts of C. obtusifolia from Tabasco and Veracruz, showed similar hypoglycemic effects (33.3% and 35.7%, respectively) and chlorogenic acid contents (Tukey(0.05) = 1.8859) (13.3 +/- 3.2 mg/g and 13.1 +/- 1.6 mg/g, respectively). The hypoglycemic effect produced by different doses (0.1, 0.25, 0.50, 0.75 and 1 g/kg body wt, p.o.) of C. peltata showed a lineal relationship with chlorogenic acid content, reaching an ED(50) = 0.540 g/kg body wt for extract, and an ED(50) = 10.8 mg/kg body wt for chlorogenic acid. These results suggest that C. peltata is a better hypoglycemic agent than C. obtusifolia, and it could be considered for developing a phytomedicinal product to carry out clinical trials.

Animals↗
PubMed1999

[Determination of baicalin and chlorogenic acid in yinhuang granule by capillary electrophoresis].

Yinhuang Granule is a kind of complex formulae made up of extract of Flos lonicerae and that of Radix scutellariae, of the fuctions of dispelling wind-heat to reduce fever, detoxicating, relieving inflammation, promoting urination and tranquilizimg the mind and can be applied to upper respiratory tract infection and tonsillitis. Baicalin and chlorogenic acid are referred to main effective components of it. In this paper, the separation and determination of baicalin and chlorogenic acid in Yinhuang Granule by capillary electrophoresis is described. The experimental conditions are stated as follow: separation column: bare fused-sillica capillary (50 microns i.d., 370 microns o.d., total length 47 cm, 40 cm from the inlet to UV detector); electrolyte: 25 mmol/L borax, pH 8.5; detection wavelength: 310 nm; sampling: 17 kPa.s; running voltage: 25 kV, polarity from anode (inlet) to cathode (outlelt); temperature: 25 degrees C; internal standard: p-nitrobenzoic acid. The linear ranges of determination for baicalin and chlorogenic acid were 160-960 mg/L(r = 0.9993, RSD = 1.76%-2.33%) and 80-960 mg/L(r = 0.9989, RSD = 1.07%-2.51%), respectively. The recoveries of baicalin and chlorogenic acid were (102.09 + 1.74)% (RSD = 1.71%, n = 6) and (99.81 + 3.11)% (RSD = 3.12%, n = 6). The method is simple, fast and accurate. It can be used for the quality control of Yinhuang Granule and its related Chinese medicines.

Anti-Infective Agents↗
PubMed1981

Potentiation of genotoxicity by concurrent application of compounds found in betel quid: arecoline, eugenol, quercetin, chlorogenic acid and Mn2+.

5 components of the betel quid were examined for their clastogenic activities individually and in various combinations. They included the alkaloid, arecoline, from the betel nut (Areca catechu L.), eugenol, from the betel vine (Piper belle L.), chlorogenic acid, from tobacco leaves (Nicotiana tabacum), quercetin, from fennel seeds (Foeniculus vulgare Mill.) and the ubiquitous transition metal Mn2+. The clastogenic effects of the concurrent applications of arecoline plus eugenol, arecoline plus quercetin and arecoline plus chlorogenic acid were greater than the sum of the action of each individual component. Similarly, the combinations of arecoline, chlorogenic acid and Mn2+ induced frequencies of chromosome aberrations which exceeded the sum of the clastogenic activities of individually applied compounds or the sum of the clastogenic activities of 2 jointly applied compounds (arecoline plus Mn2+, or chlorogenic acid plus Mn2+). The clastogenic activity was estimated as the frequency of metaphase plates with at least 1 chromatid break or chromatid exchange, or the average number of chromatid breaks and exchanges per Chinese hamster ovary (CHO) cell. A potentiating (enhancing) action was also evident when 2 clastogens were used at doses which would not lead to a detectable increase in the frequency of chromosome aberrations when applied individually. It may be useful to distinguish between a "genotoxic range", which would be applicable to individually assayed compounds, and a "cogenotoxic range", which may include concentrations at which a chemical exerts a potentiating effect when combined with other genotoxic or non-genotoxic compounds.

Animals↗
PubMed2002

The polyphenol chlorogenic acid inhibits staphylococcal exotoxin-induced inflammatory cytokines and chemokines.

Proinflammatory cytokines mediate the toxic effect of staphylococcal exotoxins (SE). Chlorogenic acid, a plant polyphenol, inhibited SE-induced T-cell proliferation (by 98%) and production of interleukin 1beta, tumor necrosis factor, interleukin 6, interferon gamma, monocyte chemotactic protein I (MCP-l), macrophage inflammatory protein (MIP)-lalpha, and MIP-lbeta by human peripheral blood mononuclear cells. These data indicate that chlorogenic acid may be therapeutically useful for mitigating the pathogenic effects of SE. Naturally occurring polyphenolic compounds such as chlorogenic acid may serve as a potent anti-inflammatory agent alternative to conventional chemotherapeutics.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1967

Allagochrome II. Effects of Light and Substrate on Allagochrome and Chlorogenic Acid Levels of Incubated Sunflower Leaf Discs.

Sunflower leaf discs incubated in the light on carbohydrate substrates exhibit several-fold increases in amounts of extractable allagochrome and chlorogenic acid. These changes are linear with time, and oxygen is required. The light effect saturates at approximately 600 muW/cm(2) "white" light, roughly the compensation point for photosynthesis. Red light is as effective as white light. Incubation in the dark, or in far red light, produces negligible changes in allagochrome and chlorogenic acid content.Sucrose (0.2 m) has been used as the standard substrate. At this concentration, glucose and fructose are slightly more effective. The optimum temperature range for incubation is 20 to 30 degrees . Allagochrome and chlorogenic acid values of both light and dark incubated samples decrease between 30 and 50 degrees , approaching zero at 50 degrees . Net light effects decrease to zero between 40 and 50 degrees .Of the inhibitors tested, 2,4-dinitrophenol, hydroxylamine and salicylaldoxime have no effect on light enhanced allagochrome and chlorogenic acid values except at high concentrations (which are generally deleterious to leaf tissues and cause decreased values for both dark and light incubated samples). Net light effects are completely inhibited, without changes in values of dark incubated discs, by azide (0.1 to 1 mm) and dichlorophenyldimethylurea (1 to 10 mum).Leaf tissues from the xantha mutant of Helianthus annuus do not exhibit a light effect. The absence of light effects in nonphotosynthetic leaf tissue and the inhibiting effects of photosynthetic poisons suggest that the photosynthetic apparatus is somehow involved.An hypothesis for light regulated metabolism via phenolic synthesis is discussed.

Journal Article↗
PubMed2005

Capillary electrophoresis with chemiluminescence detection of rutin and chlorogenic acid based on its enhancing effect for the luminol-ferricyanide system.

A capillary electrophoresis with chemiluminescence method has been developed for the determination of rutin and chlorogenic acid based on its enhancing effect on the luminol-ferricyanide system. Under the optimum conditions, the analytes could be separated within 5 min, and the detection limits of the proposed method were 0.22 microg/ml for rutin and 0.50 microg/ml for chlorogenic acid, respectively. The method was successfully applied to the analysis of rutin and chlorogenic acid in real samples.

Chlorogenic Acid↗
PubMedDate not supplied

[Chicoric and chlorogenic acids in various plants growing in Georgia].

Chicoric acid was isolated from dandelion (Taraxacum officinale Wigg.) leaves by column chromatography. Conditions for HPLC analysis of chicoric and chlorogenic acids were optimized. These acids were assayed in some plants growing in Georgia. The optimum conservation temperature for the preservation of chicoric and chlorogenic acids in leaves of dandelion and bilberry (Vaccinium arctostaphylos L.) was determined.

Asteraceae↗
PubMed2000

Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L. ).

Neochlorogenic acid (3-CQA) and cryptochlorogenic acid (4-CQA), isolated from prune (Prunus domestica L.), were identified by NMR and MS analyses. In addition, the quantity of chlorogenic acid isomers in prune were measured by HPLC. These isomers, 3-CQA, 4-CQA, and chlorogenic acid (5-CQA), were contained in the ratio 78.7:18. 4:3.9, respectively. 4-CQA was identified and quantified in prune for the first time, and relatively high amounts of this isomer were characteristic. Antioxidative activities of the chlorogenic acid isomers, such as scavenging activity on superoxide anion radicals and inhibitory effect against oxidation of methyl linoleate, were also evaluated. Each isomer showed antioxidative activities which were almost the same.

Antioxidants↗
PubMed2006

Simultaneous quantification of chlorogenic acid and caffeic acid in rat plasma after an intravenous administration of mailuoning injection using liquid chromatography/mass spectrometry.

A simple, rapid and sensitive method was developed for the simultaneous quantification of chlorogenic acid (CGA) and caffeic acid (CA) in rat plasma using a high-performance liquid chromatography system coupled to a negative ion electrospray mass spectrometric analysis. The plasma sample preparation was a simple deproteinization by the addition of two volumes of acetonitrile followed by centrifugation. The analytes and internal standard ferulic acid were separated on an Intersil C8-3 column (5 mm; 250 x 2.1 mm) with acetonitrile/0.05% triethylamine solution (70:30, v/v) as mobile phase at a flow rate of 0.2 mL/min with an operating temperature of 30 degrees C. Detection was performed on a quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source operated in selected ion monitoring (SIM) mode. Negative ion ESI was used to form deprotonated molecules at m/z 353 for chlorogenic acid, m/z 179 for caffeic acid, and m/z 193 for the internal standard ferulic acid. Linear detection responses were obtained for CGA concentrations ranging from 0.005 to 2.0 microg/mL and for CA concentrations ranging from 0.010 to 2.0 microg/mL and the lower limits of quantitation (LLOQs) for CGA and CA were 0.005 and 0.01 microg/mL, respectively. The intra- and inter-day precisions (RSD%) were within 9.0% for both analytes. Deviation of the assay accuracies was within +/-10.0% for both analytes. Their average recoveries were greater than 88.0%. Both analytes were proved to be stable during all sample storage, preparation and analytic procedures. The method was successfully applied to the pharmacokinetic study of CGA and CA following an intravenous dose of 5 mL/kg mailuoning injection to rats.

Animals↗
PubMed2004

HPLC determination and pharmacokinetics of chlorogenic acid in rabbit plasma after an oral dose of Flos Lonicerae extract.

A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of chlorogenic acid (3-O-caffeoyl-D-quinic acid) in plasma and applied to its pharmacokinetic study in rabbits after administration of Flos Lonicerae extract. Plasma samples are extracted with methanol. HPLC analysis of the extracts is performed on a C(18) reversed-phase column using acetonitrile-0.2% phosphate buffer (11:89, v/v) as the mobile phase. The UV detector is set at 327 nm. The standard curves are linear in the range 0.0500-1.00 microg/mL (r = 0.9987). The mean extraction recovery of 85.1% is obtained for chlorogenic acid. The interday precision (relative standard deviation) ranges from 5.0% to 7.5%, and the intraday precision is better than 9.0%. The limit of quantitation is 0.0500 microg/mL. The plasma concentration of chlorogenic acid shows a C(max) of 0.839 +/- 0.35 microg/mL at 34.7 +/- 1.1 min and a second one of 0.367 +/- 0.16 microg/mL at 273.4 +/- 39.6 min.

Administration, Oral↗
PubMed1996

[Determination of chlorogenic acid in Epimedium koreanum Nakai by HPLC].

The chlorogenic acid content in Epimedium koreanum was determined by HPLC. The result shows that this method is simple, specific and accurate. The recovery was 97.5% and relative standard deviation was 1.4%. With good reproducibility and wide response linearity, the method can be used for the quality control of this herbal medicine.

Chlorogenic Acid↗
PubMed1994

Effect of dietary caffeic and chlorogenic acids on in vivo xenobiotic enzyme systems.

The plant phenols chlorogenic and caffeic acids were tested for their affinity to alter hepatic and intestinal xenobiotic Phase I and Phase II enzyme activities in mice. Mice were fed isocaloric and isonitrogenous powdered diets containing 0 and 0.2% caffeic and chlorogenic acids, respectively. Animals pre-treated with benzo(a)pyrene (B(a)P) were sacrificed 18 h after an oral dose (50 mg/kg Bwt). B(a)P induced animals exhibited higher (p < 0.05) microsomal AHH, UDPGT, P-450 and cytosolic GST liver enzyme activities. The presence of these phenolics in the diet was shown to have little effect in modulating hepatic xenobiotic activating-detoxification enzymes. The dietary intake of caffeic and chlorogenic acids was particularly evident with intestinal xenobiotic AHH and GST enzyme activity. These data indicate that the presence of caffeic and chlorogenic acids in the diet may have an integral role in modulating the carcinogenic potential of reactive xenobiotics such as B(a)P.

Administration, Oral↗
PubMed1992

The inhibitory action of chlorogenic acid on the intestinal iron absorption in rats.

The polyphenols are part of the composition of many foods, it is known the inhibitory effect of tea and coffee through the tannins on iron intestinal absorption; the "yerba mate" (Ilex Paraguarensis) is a beverage widely used in South America, that has a high content of a polyphenol named chlorogenic acid. The present work shows the effect of this substance in nonhem iron absorption. An intestinal loop, was made in rats, to form a closed cavity in a small section of intestine tieing it from the pilorous to a distance of six cm. In this closed cavity a solution of 59Fe was injected with different doses of chlorogenic acid; it was living 20, 40 and 120 minutes into the loop, and after this different times, the blood, spleen, liver, femur and intestine were removed to measure the 59Fe uptake to be compared with the control group. The results gave an intense inhibitory effect on the intestinal iron absorption with doses of 0.58 and 1.7 mM per rat of chlorogenic acid at the different times studied.

Animals↗
PubMed2004

Effect of domestic cooking on human bioavailability of naringenin, chlorogenic acid, lycopene and beta-carotene in cherry tomatoes.

BACKGROUND: Epidemiological data showed that tomato and tomato product (sauce, paste) consumption is associated with a protective effect against the development of some chronic-degenerative diseases. Tomato antioxidant bioactive molecules such as carotenoids and polyphenols could be responsible, at least in part, for the healthy effect observed. The bioavailability of these compounds is an essential requirement to sustain their in vivo role. While it is well known that many factors can influence the bioaccessibility of carotenoids from the food matrix, there is little information about the factors affecting phenolic compounds' bioaccessibility. AIM OF THE STUDY: This investigation was carried out to evaluate the effect of domestic cooking on the bioavailability in humans of antioxidant molecules after the administration of a test meal containing cherry tomatoes. METHODS: A cross-over design was conducted. Subjects (3 females and 2 males) consumed experimental meals containing fresh and cooked cherry tomatoes. Blood collection was performed at different time intervals (0, 2, 4, 6, 8 and 24 h). RESULTS: Carotenoid and phenol plasma concentrations were measured. Plasma levels of lycopene and beta-carotene were not significantly different with respect to the baseline after ingestion of both the test meals, while plasma concentrations of naringenin and chlorogenic acid increased significantly with respect to the baseline (P<0.05) after administration of cooked cherry tomatoes, but not after administration of fresh cherry tomatoes. CONCLUSIONS: The present study indicated that domestically cooked tomatoes significantly increase naringenin and chlorogenic acid plasma levels. Considering that both naringenin and chlorogenic acid are widely studied for their potential healthy properties, evidence of their bioavailability and of the factors influencing their bioaccessibility is an important tool to sustain the possibility that these polyphenols play a biological role in human physiology.

Adult↗
PubMed2004

Lost P1 allele in sh2 sweet corn: quantitative effects of p1 and a1 genes on concentrations of maysin, apimaysin, methoxymaysin, and chlorogenic acid in maize silk.

In the United States, insecticide is used extensively in the production of sweet corn due to consumer demand for zero damage to ears and to a sweet corn genetic base with little or no resistance to ear-feeding insects. Growers in the southern United States depend on scheduled pesticide applications to control ear-feeding insects. In a study of quantitative genetic control over silk maysin, AM-maysin (apimaysin and methoxymaysin), and chlorogenic acid contents in an F2 population derived from GE37 (dent corn, P1A1) and 565 (sh2 sweet corn, p1a1), we demonstrate that the P1 allele from field corn, which was selected against in the development of sweet corn, has a strong epistatic interaction with the a1 allele in sh2 sweet corn. We detected that the p1 gene has significant effects (P < 0.0001) not only on silk maysin concentrations but also on AM-maysin, and chlorogenic acid concentrations. The a1 gene also has significant (P < 0.0005) effects on these silk antibiotic chemicals. Successful selection from the fourth and fifth selfed backcrosses for high-maysin individuals of sweet corn homozygous for the recessive a1 allele (tightly linked to sh2) and the dominant P1 allele has been demonstrated. These selected lines have much higher (2 to 3 times) concentrations of silk maysin and other chemicals (AM-maysin and chlorogenic acid) than the donor parent GE37 and could enhance sweet corn resistance to corn earworm and reduce the number of applications of insecticide required to produce sweet corn.

Alleles↗
PubMed1979

[Influences of food browning on nutrition. II. Effects of ingestion of non-dialyzable browning substances, dopamelanin and chlorogenic acid on growth and protein and lipid metabolism in rats (author's transl)].

In a previous paper, we reported an inhibitory effect on growth rate and plasma lipid composition in rats of browning substances obtained from ethanol extraction of minced egg plant. In order to know active principle(s) in browning substances, attempts were made to condense the substances and chlorogenic acid was separated. The ingestion of chlorogenic acid and other browning substances such as dopameranin and catechin were examined for their biological activity. Active principle(s) in the browning substances appeared to interfere with digestion and utilization of dietic protein. The principle resided in dialyzable fraction in enzymatically produced materials and in nondialyzable fraction in materials obtained by chemical oxidation. Ingestion of nondialyzable fraction caused an elevation of total serum cholesterol level, but little change in serum fatty acid composition.

Animals↗
PubMed2006

The blood pressure-lowering effect and safety of chlorogenic acid from green coffee bean extract in essential hypertension.

Chlorogenic acids (CGA) in green coffee bean extract (GCE) reduce blood pressure in spontaneously hypertensive rats and humans. The authors examined the blood pressure-lowering effect and safety of CGA in patients with mild hypertension through a placebo-controlled, randomized clinical trial. Subjects (n = 28) were randomized to receive treatment with CGA (140 mg/day) from GCE or placebo. Blood pressure, pulse rate, body mass index, routine blood test, hematochemistry, urinalysis, and subjective symptoms were recorded throughout the study. In the CGA group, but not the placebo group, blood pressure (systolic and diastolic) decreased significantly during the ingestion period. There was no difference in body mass index and pulse rate between groups, nor were there any apparent side effects. Thus, CGA from GCE is effective in decreasing blood pressure and safe for patients with mild hypertension.

Adult↗