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Influence of the beehive type on the quality of honey.

Agricultural producers apply numerous technological procedures, and enlarging efforts to produce the high-quality products. This initiative is present in the beekeeping, too. The quality of the honey produced by the honey bee colonies depends of various factors, but prevailing are the ecological conditions and the floristic composition of the honeyfull plants. The aim of our research was to discover the influence of the beehive type on the quality of honey, which is produced at apiaries under the similar environmental conditions. The whole studied honey bee colonies belong to the European race, Apis mellifera carnica, and they used the same honeyfull plants pastures. The results indicate that different beehive type used at apiaries influenced on the quality of honey.

Agriculture↗

[Honey-induced poisoning].

Because of the increasing preference for natural products intoxications induced by consumption of honey will reappear, especially with products bought directly from the beekeeper. In the hospital of Trapezunt about 8 cases of intoxications induced by honey were reported per year. The courses observed appear to be identical to those already described by Xenophon 2400 years ago. Symptoms begin acutely but last rarely for more than 24 hours. Fatal cases are extremely rare. Most prominent symptoms are loss of consciousness, weakness, severe salivation, sweating, vomiting and diarrhea. Beside these symptoms circumoral paresthesias and bradyarrhythmia may occur. Intoxication is induced by certain diterpenes, so called gray-anotoxins, that appear in flowers of different species of rhododendron. Next to close surveillance only symptomatic therapy is generally necessary.

Diterpenes↗

Mineral content of the honey produced in Zulia state, Venezuela.

The mineral content of the honey produced in five zones of the Zulia state, Venezuela, during dry and rainy seasons was determined. The analyzed elements were: sodium, potassium (by emission spectroscopy), calcium, magnesium, copper, iron, manganese (by atomic absorption spectroscopy), phosphorus (phosphate ions, by colorimetric method), and ash content of raw honey samples directly collected from different beekeepers. The mean values for Na, K, Ca, Mg, Cu, Fe, Mn, and P were 353+84; 1774+138; 237+66; 52+24; 0.76+0.43; 13.5+10.23; 0.92+0.42 and 1642+323 mg/kg respectively. The mean ash content was 0.431+0.15%. Potassium was the most abundant of the elements determined. This results confirm that Zulian honey can be considered a good source of minerals.

Animals↗

Effect of honeybee broods (queen-bee different lineage) moving on disease development at various beehive types and allergy reaction cause in humans.

The change of the location of the beehives on the diverse honeyfull pastures is the usual apiarists activity in the Republic of Croatia. The main reasons are the climatic and vegetation diversity, and richness of the floral composition, with numerous bee forage plants. Our study aimed to detect consequences of honeybee broods (Queen-bee of different lineage) moving, from one habitat to another at various type of beehives. The Alberti-Znidersic (AZ), Langstroth-Root (LR) and Dadant-Blatt (DB) beehive types, constructed of (lime-tree), have been used. After the bee forage on the Oilseed Rape in the beginning of April, the honeybee brood has been veterinary inspected (based on the Law of animal health protection in the Republic of Croatia) for varrosis, nosemosis and American foulbrood diseases. The same procedure was done after bee forage (False acacia) at the end of May. All of the honeybees belong to the European race Apis mellifera carnica. The results of the study pointed out that different beehive types and the Queen-bee lineage (natural and selected) affect development of disease inside the honeybee brood, during the relocation and change from one dominant bee forage plants to another. Certain allergy reactions occurring in people can be caused by the pollen of some honefull plants such as birch, grasses, ragweed, goldenrod and hazel. Such cases are also included in our investigations. Beekeepers and nature lovers sensitive to pollen allergens of some honeyfull plants should, in some calendar period, avoid ecological milieu with such plants.

Allergens↗

Immune response to bee venom. II. Quantitation of the absolute amounts of IgE and IgG antibodies by saturation analysis.

We have measured in allergic and non-allergic beekeepers IgE and IgG antibodies to the bee venom allergens, phospholipase A2 (PLA2) and hyaluronidase (HYAL), by the radioallergosorbent test (RAST) and a 125I-radiolabelled antigen-binding assay. The absolute amount of IgG antibody in a reference serum was determined by saturation analysis using 125I-radiolabelled PLA2 and HYAL. Using monoclonal anti-IgE coated microtitre plates, the absolute amount of IgE antibody to the same antigen was also determined by saturation analysis. Regardless of the IgE response to the different allergens, IgG antibody concentrations to PLA2 were invariably higher than those to HYAL. In addition, the ratio of IgG to IgE antibody was higher for PLA2 (220:1) than for HYAL (10:1). Higher levels of IgG antibody to both allergens (especially HYAL) were found in those who had had prolonged exposure to bee stings. These data suggest that the level of IgG antibody produced is related to the dose administered, while the amount of IgE antibody may be regulated by other factors.

Antibody Specificity↗

Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response.

Labeled antigen-binding tests were used to determine quantitatively the contribution of IgG4 antibodies to the total IgG antibody response in humans. In agreement with literature, we found no IgG4-restricted antibody responses with tetanus toxoid or streptococcal carbohydrate. In the serum of individuals immunized for several years with phospholipase (PLA) from honey bee venom, grass pollen allergen, or house dust mite allergen, we often found that more than 50% of the total antigen-binding capacity was due to IgG4 antibodies. In the case of beekeepers, it could clearly be shown that during prolonged immunization a shift in the IgG4:IgG1 antibody ratio occurs that finally results in an IgG4-dominated antibody response. Evidence is provided that antigen-binding assays may even underestimate the contribution of IgG4 antibodies, because in contrast to IgG1 antibodies, IgG4 antibodies act as monovalent antibodies in being unable to cross-link immunosorbent-bound antigen and radiolabeled antigen.

Antibodies, Anti-Idiotypic↗

Effects of passive antibody in bee venom anaphylaxis.

Twelve patients allergic to honeybees were challenged by injections of bee venom; five responded systemically to the venom, with symptoms ranging from angioedema to respiratory distress. These patients were given intramuscular or intravenous infusions of gamma-globulin obtained from the plasma of hyperimmune beekeepers who had high levels of antibody to an allergen (phospholipase A) in the venom. Post-infusion, all five patients tolerated 1.5 to 5 times the venom dose that previously elicited adverse reactions. The quantity of passive IgG antibody infused did not impair the patient's own immune response to venom. These results represent the best available evidence for a direct role for IgG blocking antibodies in clinical protection against anaphylaxis occurring as a result of parenteral antigenic challenge as may be observed in penicillin and insect hypersensitivity.

Adolescent↗

Clinical and immunologic surveys of Hymenoptera hypersensitivity in Japanese forestry workers.

BACKGROUND: The increased incidence of bee-sting allergy among beekeepers is well known, whereas Hymenoptera hypersensitivity among forestry workers, who are frequently stung by Hymenoptera, is not well understood. OBJECTIVE: To elucidate the nature of Hymenoptera hypersensitivity among forestry workers and analyze the mechanism involved in Hymenoptera-induced systemic reactions. METHODS: A questionnaire was administered and yellow jacket venom-specific IgE antibody titers measured in 323 forestry workers and 100 age-matched office workers with little occupational exposure to Hymenoptera stings as a control group. RESULTS: From the questionnaire it was found that the percentage of systemic reactions to Hymenoptera stings was significantly greater in the forestry workers than in the control subjects (P < .01). The number of sting exposures was significantly higher in the forestry workers than in the control subjects (P < .01) and was closely correlated with episodes of systemic reaction. Yellow jacket venom-specific IgE antibody titers were significantly higher in forestry workers with episodes of systemic reaction compared with those with only local or large local reactions to Hymenoptera (P < .05), although more than half of these subjects had undetectable IgE antibody titers to yellow jacket venom. IgE antibody titers against yellow jacket venom were closely correlated with those of paper wasp (rs = .802, P < .001), which is another frequent source of Hymenoptera stings. In workers with episodes of sting exposure within the most recent 3 years, the period from the last sting was not correlated with yellow jacket venom-specific IgE antibody titers. CONCLUSIONS: The number of sting exposures may contribute to systemic reactions to Hymenoptera sting, and some mechanism other than IgE mediated hypersensitivity may also be involved in Hymenoptera-induced systemic reactions.

Adult↗

[Is identification of badly burned cadavers impossible? Reconstruction of the burned facial skull as a basis for roentgen identification].

The described case underlines all difficulties which arise in identification procedure of totally burned human remains. The main problem is the fragility of bone and tooth fragments and the radiological screening of structures of high individual specificity. After the fire in an old beekeeping a totally burned corpse has been found. The fragments of the bones and jaws were fixed with sodium silicate immediately. The viscero-cranium was reconstructed and x-rayed in an modified orthopantomography-x-ray-equipment. The comparison of pre- and postmortem radiographs made a positive identification possible.

Burns↗