Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “BLOOD PLASMA”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 127 records · Page 7Linked to original sources
PubMed1997

Determination of the PCO2-dependent component of the H+ concentration in venous and arterial blood plasma.

In normal venous blood plasma, the regression line of [H+] plotted against the PCO2 was linear against the square root of PCO2. Sequential measurements in venous and arterial blood of PCO2 and [H+] showed that the venous-arterial (V-A) difference in [H+] was linearly related to the V-A difference in the square root of PCO2, the regression line having the same slope as that of the venous [H+] plotted against the square root of PCO2. These findings suggested that the venous [H+] on the regression line represents the PCO2-dependent component, of [H+], [H+]*. The PCO2-independent component, delta [H+], can then be given by subtracting [H+]* from the measured [H+]. The delta [H+] in venous blood agreed well with that in arterial blood with a correlation coefficient of 0.99, supporting the validity of the value of [H+]*.

Adult↗
PubMed1982

Seminal plasma free amino acids and seminal and blood plasma proteins of the guinea fowl (Numidia meleagris).

Glutamic acid was the major free amino acid in guinea seminal plasma, representing 90.00% of the total amino acids. Threonine comprised 3.74%, aspartic acid 3.23%, and serine 1.50%. Others were detected at less than .50% of the total free amino acids. Blood plasma protein concentration and SE were 2.68 +/- .35 g/100 ml, and the proteins were fractionated by disc acrylamide electrophoresis into seven major bands: albumin, alpha-1, a dense beta fraction, and four postbeta, or gamma, proteins. Seminal plasma protein concentration and SE were .45 +/- .03 g/100 ml, and the proteins separated into nine fractions: albumin, alpha-1 and -2, beta-1, -2, and -3, and three postbeta, or gamma, bands.

Amino Acids↗
PubMedDate not supplied

[Determination of the antiactivator activity in human and animal blood plasma using tissue plasminogen activator].

An antiactivatory effect was estimated in blood plasma of human donors, rats and dogs on plates of stabilized fibrin using tissue plasminogen activator from pig heart. Inhibition of the tissue activator, used at definite concentrations, was expressed in per cents. The antiactivatory effect was distinctly higher in rat blood plasma, whereas it was similar in human and dog blood plasma, being about 3-fold decreased as compared with rats. The antiactivatory effect did not correlate with the antiactivator content in all the blood plasma preparations studied.

Animals↗
PubMed1986

Increase in blood plasma levels of boron and strontium in hemodialyzed patients.

Boron and strontium concentrations in blood plasma of controls and hemodialyzed patients from two Centers were determined by inductively coupled plasma emission spectrometry. Boron concentrations in blood plasma were respectively, in controls 2.6 +/- 0.9 mumol/l and in hemodialyzed patients 16.1 +/- 5.6 mumol/l before the dialysis session and 9.5 +/- 3.2 mumol/l at the end. The decrease in blood plasma during the dialysis was concomitant with an increase in the dialysis fluid (1.2 +/- 0.7 mumol/l at the beginning and 4.6 +/- 1.8 mumol/l at the end). Strontium concentrations in blood plasma were respectively, in controls 0.22 +/- 0.06 mumol/l and in hemodialyzed patients 0.62 +/- 0.24 mumol/l before the dialysis session and 0.64 +/- 0.14 mumol/l at the end. The mean concentration of strontium in the dialysis fluid was the same before (0.49 +/- 0.11 mumol/l) and after the dialysis session (0.49 +/- 0.10 mumol/l), but a transfer between plasma and dialysis fluid was shown by individual changes. Some considerations about these results are put forward but their possible clinical consequences are not yet known.

Adult↗
PubMed1981

[Insulin and cortisol levels in blood plasma and adrenal gland mass in swine during the perinatal period].

The level of immunoreactive insulin activity and glucose in blood plasma of the umbilical vein was found to be lower than that in maternal venous blood plasma, in the last third of intra-uterine development, that is between the 80th and 112th day. No age-dependent differences were recordable regarding immunoreactive insulin activity in foetal blood plasma. Concentrations of glucose and immunoreactive insulin in venous blood plasma of piglets were found to rise, within 24 hours from birth, due to milk intake. Such rises were statistically significant but differed by individuals. None of the above parameters increased in fasting piglets. Cortisol in foetal blood plasma was found to be low, usually less than 5 ng/ml, on the 80th, 100th, and 106th day of intra-uterine life, but it went up between the 106th and 112th day. The highest concentrations were recorded from the oldest foetuses, 114 day of intra-uterine development. The cortisol levels in the blood plasma of the mothers of the above mentioned foetuses varied between 5 ng/ml and 70 ng/ml. Relative adrenal gland weight in animals aged 112 and 114 days was found to be significantly higher than that in foetuses aged 80, 100, and 106 days. No significant postnatal change in relative adrenal gland weight was recorded from piglets in the first nine days of life.

Adrenal Glands↗
PubMedDate not supplied

[Effect of the intravenous administration of homologous albumin on the lipolytic enzyme activity of rabbit blood plasma].

Activity of main lipolytic enzymes of rabbit blood plasma, lipoprotein lipase (LPL) and liver tissue triglyceride lipase (L-TGL) as well as intensity of lipoprotein lipolysis in vivo, which was estimated by an increase of unesterified fatty acids in blood plasma, studied after intravenous administration of homologous albumin at a dose of 200 mg/kg of body mass. The data obtained suggest that albumin is involved in regulation of lipoprotein lipolysis due to activation of LPL, caused by asseption of unesterified fatty acids, which are liberated after hydrolysis of triglycerides. This effect was exhibited by albumin only under conditions of active stimulation of lipolytic reactions, it occurs after intravenous administration of heparin (100 IU/kg of body mass). Albumin did not activate lipoprotein lipolysis and lipolytic enzymes in intact animals. Activity of L-TGL in blood plasma was less distinctly subjected to the regulatory effect of albumin.

Albumins↗
PubMed2003

Computer simulation of Zn(II) speciation and effect of Gd(III) on Zn(II) speciation in human blood plasma.

The speciation and distribution of Zn(II) and the effect of Gd(III) on Zn(II) speciation in human blood plasma were studied by computer simulation. The results show that, in normal blood plasma, the most predominant species of Zn(II) are [Zn(HSA)] (58.2%), [Zn(IgG)](20.1%), [Zn(Tf)] (10.4%), ternary complexes of [Zn(Cit)(Cys)] (6.6%) and of [Zn(Cys)(His)H] (1.6%), and the binary complex of [Zn(Cys)2H] (1.2%). When zinc is deficient, the distribution of Zn(II) species is similar to that in normal blood plasma. Then, the distribution changes with increasing zinc(II) total concentration. Overloading Zn(II) is initially mainly bound to human serum albumin (HSA). As the available amount of HSA is exceeded, phosphate metal and carbonate metal species are established. Gd(III) entering human blood plasma predominantly competes for phosphate and carbonate to form precipitate species. However, Zn(II) complexes with phosphate and carbonate are negligible in normal blood plasma, so Gd(III) only have a little effect on zinc(II) species in human blood plasma at a concentration above 1.0 x 10(-4) M.

Computer Simulation↗
PubMed1989

[Total estrogen concentrations in the blood plasma of calves during the first six days of life].

From 47 mature calves ten blood samples were taken during the first six days of life for determination of total estrogen concentrations. Immediately postnatal concentration of total estrogens in blood plasma was high (1093.2 +/- 452.5 pg/ml blood plasma). Total estrogen values increased towards the sixth hour of life (1789.0 +/- 892.2 pg/ml blood plasma), which is thought to be caused by reabsorption of estrogens released from intracorporeal reservoirs. Towards the 48th hour of life total estrogen values decreased. This decrease indicates a progressive depletion of the reservoirs and also metabolisation and elimination of estrogens by the organism. The fact that total estrogens were nearly unchanged from the 4th day of life onwards reveals the approximative completed elimination of estrogens. At no time significant differences of total estrogen concentrations in blood plasma were found between female and male newborn calves.

Animals↗
PubMed1979

Blood-surface interactions: Fourier transform infrared studies of protein surface adsorption from flowing blood plasma and serum.

The adsorption of blood proteins onto various surfaces in contact with flowing blood plasma or serum has been investigated using Fourier Transform infrared spectroscopy (FT-IR) coupled with attenuated total reflection (ATR). Comparison of adsorption from a static versus a flowing blood plasma system indicated that a greater amount of protein was adsorbed when the static system was employed, but desorption was observed with the static system and not in the flowing system. When plasma and serum were compared in terms of amount and type of protein adsorbed, little difference was noted in either kinetics of adsorption or stability of the adosrbed layers. Variations in the ratios of infrared adsorption bands (from either plasma or serum) indicate that changes occur during the adsorption process in the protein layers. A comparison of poly(vinyl chloride) to germanium as the protein adsorbing surface in the static system showed differences in terms of the composition of the adsorbed layers.

Adsorption↗
PubMed1975

Certain physiochemical properties of uterine tubal fluid, follicular fluid, and blood plasma in the mare.

Uterine tubal fluids were collected twice a day from mares for 5 consecutive estrous cycles between March 15 and September 1. Follicular fluids were aspirated from the follicles of exteriorized ovaries of 3 mares between days 2 and 5 of estrus. Uterine tubal fluid and follicular fluid were analyzed for osmolarity, dry matter, total lipids, total free fatty acids, glucose, fructose, and lactic acid. Blood samples were collected (jugular venipuncture) throughout the estrous cycle, and the same physical and biochemical analyses were made on blood plasma. A difference (P less than 0.01) was found for osmolarity between uterine tubal fluids collected during estrus and those collected during anestrus. The osmolarity of uterine tubal fluid during anestrus was greater than that of blood plasma; follicular fluid was similar in osmolarity to blood plasma. The dry matter in blood plasma was greater (P less than 0.01) than that in either uterine tubal fluid or follicular fluid. Cyclic variations in dry matter content were not observed in uterine tubal fluid. Total lipids in blood plasma and follicular were greater (P less than 0.01) than those in uterine tubal bluid. The concentration of total lipid in uterine tubal fluid was similar during estrus and anestrus. Myristic acid (C14:0) in blood plasma and myristoleic acid (C14:1) in uterine tubal fluid were the only free fatty acids that had cyclic variation. The fatty acids in the greatest concentration in uterine tubal fluid and blood plasma were palmitic acid (C16:0) and linoleic acid (C18:2). Concentrations of linoleic acid and stearic acid (C18:0) were greater (P less 0.01) in follicular fluid than in uterine tubal fluid or blood plasma. Only trace amounts of glucose were detected in uterine tubal fluid, whereas a considerable amount of glucose was found in follicular fluid. Fructose was not detected in any of the fluids. Lactic acid concentrations did not differ between estrus and anestrus. Lactic acid concentration was significantly greater (P less than 0.01) in uterine tubal fluids and follicular fluids than in blood plasma.

Animals↗
PubMedDate not supplied

[Quantification of D-dimer and soluble fibrin in blood plasma of people with ischemic heart disease and hypertension].

The method of D-dimer quantification in the human blood plasma has been developed using monoclonal antibodies 111-3b and II-4d. The method has been verified on the blood plasma of the patients with ischemic heart disease with and without stenocardia and with hypertension. The results showed that at ischemic heart disease with and without stenocardia and at hypertension the quantities of D-dimer in the blood plasma were generally less than the highest normal level 500 ng/ml (64.3%, 76.2% and 95%, correspondingly). The semiquantitative measurements of soluble fibrin levels in blood plasmas of the patients with ischemic heart disease and hypertension have been performed. It has been shown that the quantity of soluble fibrin at these diseases range greatly from < 0.03 mg/ml to 0.15 mg/ml. There was no correlation between the quantities of D-dimer and soluble fibrin in blood plasmas of the patients. Electrophoresis in PAAG with SDS showed that the soluble fibrin at these diseases had the mo- lecular mass of the fibrin (ogen). Thus the soluble fibrin in blood plasmas analysed consisted mainly of fibrin desAA oligomers (may be with fibrinogen incorporation) which are not stabilized by the factor XIIIa.

Blood Protein Electrophoresis↗
PubMedDate not supplied

[Dynamics of changes in the protease-inhibitory balance in blood plasma of dogs with experimental acute pancreatitis].

Changes of proteinase-inhibitory system values in blood plasma of dogs with experimental acute pancreatitis (EAP) were studied in dynamics. Their diagnostic and prognostic values was appraised. Essential shifts were revealed in the proteinase-inhibitory balance in blood plasma of dogs with EAP of various severity. The concentration of trypsin- and chymotrypsin-like proteinases exceeded the normal values 1.8-3.3 times in these animals. Coefficients expressing the relation of antitryptic and antichymotryptic activities to the activities of the corresponding proteinases in the blood plasma of animals with EAP were indicative of diminished blood plasma inhibitory potential as compared to that in intact animals. The authors are the first to show the diagnostic and prognostic value of the antichymotryptic activity/chymotrypsin in blood plasma of dogs with EAP.

Acute Disease↗
PubMed1993

Blood plasma pseudouridine in patients with malignant proliferative diseases.

The blood plasma concentration of pseudouridine was estimated in 104 healthy adult subjects, and 108 patients suffering from malignant proliferative diseases. The HPLC method for simultaneous determination of pseudouridine and creatinine was applied. The average physiological concentration of pseudouridine in blood plasma was 2.43 +/- 0.97 mumol.l-1 or 29.15 +/- 7.40 mmol.mol-1 creatinine. The physiological urinary excretion of pseudouridine was 14.32 +/- 5.20 mumol.24 h-1.kg-0.75 or 19.60 +/- 5.22 mmol.mol-1 creatinine. Renal clearance of pseudouridine and endogenous creatinine were 4.04 +/- 0.99 and 5.50 +/- 1.46 ml.kg-0.75, respectively. A positive correlation (r = 0.55, P < 0.01) was found between age (in the range 20-92 years) and blood plasma pseudouridine concentration (mumol.l-1). By expressing plasma pseudouridine in relation to plasma creatinine, the apparent influence of non-metabolic factors (age, renal insufficiency, blood dilution) on the plasma pseudouridine concentration were largely excluded. Among haematological proliferative diseases the highest values of plasma pseudouridine concentrations were observed in chronic lymphocytic leukaemia (8.19 mumol.l-1; 54.9 mmol.mol-1 creatinine) and multiple myeloma (7.02 mumol.l-1; 52.5 mmol.mol-1 creatinine). In multiple myeloma, but not in chronic lymphocytic leukaemia, the plasma pseudouridine concentration depended on the clinical stage. A lower, but still significant response in non-Hodgkin's lymphoma was noted (4.03 mumol.l-1; 40.88 mmol.mol-1 creatinine). A significant increase of the plasma pseudouridine concentration was characteristic of adenocarcinomas of the large intestine, and it occurred in the early stages of malignant growth. In patients with lung cancer the plasma pseudouridine concentration was elevated only in advanced cases with metastases.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

The effect of carbidopa on the pharmacokinetics and metabolism of intravenously administered levodopa in blood plasma and skeletal muscle.

The effect of carbidopa on the pharmacokinetics and metabolism of levodopa (L-dopa) in blood plasma and skeletal muscle extracellular fluid (ECF) has been studied by repeated measurements in one beagle dog. The administration of a single dose of L-dopa (25 mg/kg i.v.) without carbidopa pretreatment (controls) resulted in an increase in the concentrations of L-dopa and 3-O-methyldopa (3-OMD) in blood plasma and skeletal muscle ECF dialysates. This effect was clearly potentiated for L-dopa in blood plasma (186% increase in AUC) and 3-OMD in skeletal muscle dialysates (108% increase in AUC) after pretreatment with carbidopa (100 mg/day). In addition, carbidopa prolonged the half-life of the elimination of L-dopa in blood plasma by 48% and in skeletal muscle ECF by 66% but did not influence its blood plasma distribution half-life (t 1/2 alpha = 0.17 h). The elimination half-life of L-dopa in the controls was higher in muscle (t 1/2 beta = 1.76 h) than in blood plasma (t 1/2 beta = 0.50 h). Carbidopa pretreatment resulted in a relatively small increase (29%) in the L-dopa content of skeletal muscle ECF as indicated by the AUC. The accumulation of 3-OMD in muscle dialysates, in contrast to that in plasma, was significantly enhanced after the administration of L-dopa following treatment with carbidopa. In the control experiments, dopamine (DA) was detectable only in the dialysates from muscle ECF.(ABSTRACT TRUNCATED AT 250 WORDS)

3,4-Dihydroxyphenylacetic Acid↗
PubMed1988

[Changes in the RNA content of the blood plasma in rats with Ehrlich ascitic cancer].

The RNA content in the blood plasma of tumour-bearing animals correlates with the tumour growth stage. The development of Ehrlich carcinoma in rats is followed by the RNA increase in the blood plasma, while spontaneous regression of the tumour is accompanied by a decrease of the RNA content in the blood plasma of rats with Ehrlich carcinoma almost to the normal level. The fractional spectrum of the blood plasma RNA in the tumour bearing rats is similar to that of the cell-free ascitic fluid of rats.

Animals↗
PubMed1995

The effect of carbidopa and entacapone pretreatment on the L-dopa pharmacokinetics and metabolism in blood plasma and skeletal muscle in beagle dog: an in vivo microdialysis study.

The effects of carbidopa and entacapone pretreatment on the pharmacokinetics and metabolism of i.v. administered L-3,4-dihydroxyphenylalanine (L-dopa) have been examined in vivo in blood plasma and skeletal muscle extracellular fluid (ECF), in beagle dog, by microdialysis. Both with or without carbidopa, blood plasma L-dopa levels declined biexponentially after the i.v. administration of L-dopa. In contrast to blood plasma, a monoexponential decline was observed in muscle ECF in both these pharmacological conditions. Pretreatment with carbidopa had no significant effect on the pharmacokinetic parameters of L-dopa in blood plasma, but resulted in an increase in the area under the concentration versus time curve (AUC) and elimination half-life (t1/2) of L-dopa in muscle ECF (0.61 hr), compared with values achieved after L-dopa alone. Carbidopa pretreatment enhanced the accumulation of 3-O-methyldopa (3-OMD) and dopamine (DA) in muscle ECF but decreased that of L-3,4-dihydroxyphenylacetic acid (DOPAC) in both blood plasma and muscle ECF. Entacapone had a pronounced inhibitory effect on the formation of 3-OMD, resulting in a reduction of the AUC for 3-OMD by 98% and 85% in blood plasma and muscle ECF, respectively. Pretreatment with carbidopa plus entacapone enhanced the Tmax of L-dopa in muscle ECF compared with the values achieved after pretreatment with carbidopa alone. In addition, the elimination half-life (2.66 hr) and volume of distribution by area of L-dopa in blood plasma and its AUC and t1/2 in muscle ECF (1.80 hr) were enhanced substantially. No DA was detected, but DOPAC levels were enhanced in both blood plasma and muscle ECF. These results suggested that carbidopa has a L-dopa-sparing effect in skeletal muscle, which is further enhanced by entacapone.

Animals↗
PubMed1994

Polyamines and pyrimidines in blood plasma and urine of dogs bearing mammary tumours.

The blood plasma and urinary pattern of polyamines and pyrimidines in dogs bearing mammary tumours was examined. A large variability of pyrimidines in blood plasma and spermidine, spermine and pseudouridine in urine of healthy and tumour-bearing dogs was observed. The blood plasma level of uracil and uridine as well as urinary concentration of pseudouridine and spermidine/spermine ratio were significantly elevated in dogs with mammary tumours.

Animals↗
PubMed1980

Comparison of milk and blood plasma progesterone concentrations in cycling and pregnant mares.

Progesterone concentrations were measured in milk and blood plasma for 15 mares throughout a normal estrous cycle and early pregnancy to determine the feasibility of utilizing progesterone in milk as an indicator of pregnancy. Samples were obtained daily from foaling until diagnosis of pregnancy by rectal examination at 30 to 35 days of gestation. Progesterone in milk and blood plasma was quantified by radioimmunoassay. Mean progesterone concentrations (+/- SE) in plasma from foaling to foal heat and during estrus, luteal phase and pregnancy were .51 +/- .09 ng/ml, .53 +/- .08 ng/ml, 3.88 +/- .26 ng/ml and 4.22 +/- .09 ng/ml, respectively; progesterone concentrations in milk during the same periods were 1.57 +/- .16 ng/ml, 1.41 +/- .06 ng/ml, 4.26 +/- .21 ng/ml and 4.14 +/- .09 ng/ml respectively. Progesterone concentrations in milk were higher than concentrations in plasma only from foaling to foal heat and during estrus. Days of the estrous cycle on which milk and (or) blood plasma progesterone concentrations could be used to detect pregnancy were determined. Progesterone concentrations in milk from seven of the mares on each day from day 1 to 25 of a normal estrous cycle (nonpregnant) were compared to progesterone concentrations in milk from the same seven mares on days 1 to 25 of a subsequent fertile estrous cycle (pregnancy). (The first day that mares refused to accept the stallion respectively.) Identical comparisons were made of progesterone concentrations in blood plasma. Significantly lower progesterone concentrations were observed in milk from nonpregnant mares from days 17 to 22 and in blood plasma from nonpregnant mares from days 15 to 22.

Animals↗