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Effect of soaking in Hank's balanced salt solution or milk on PDL cell viability of dry stored human teeth.

This study was designed to evaluate the effect of soaking in either Hank's balanced salt solution (HBSS) or milk on periodontal ligament (PDL) cell viability in avulsed teeth. Dry storage times of 30, 60, and 90 min were evaluated. PDL cell viability was determined after removal of the cells from the root surfaces of extracted teeth using a modification of the procedure described by Nakashima (Arch Oral Biol 1991;36:655-63). After trypsinization and subsequent treatment in collagenase, the cells were stained with trypan blue, and viable and non-viable cells were counted using a hemocytometer and converted to percentages for statistical comparison. The results of this study demonstrated no significant difference in the number of viable cells with or without soaking in HBSS or milk at any of the dry storage times. In addition, there was no significant difference in PDL cell viability between the 30-and the 60-min dry periods. Although the soaking procedure had no obvious negative consequence, no significant improvement in PDL cell viability by the addition of this step was demonstrated under the conditions of this study.

Adolescent↗

An oral health promotion program for the prevention of complications following avulsion: the effect on knowledge of physical education teachers.

The aim was to assess the knowledge levels of physical education teachers before and after a seminar, presented by dental faculty as part of a community outreach program, in which the need for immediate treatment because of avulsion of permanent teeth has been emphasized, and to compare knowledge levels of teachers who attended the seminar with those who did not. Physical education teachers attended a seminar presented by senior faculty of the Hebrew University-Hadassah School of Dental Medicine as part of an educational campaign in the community. The seminar included clear instructions on the appropriate treatment of avulsed permanent teeth, which were appropriate for physical education teachers. The teachers completed two multiple-choice self-administered anonymous questionnaires related to immediate treatment they could provide in cases of permanent teeth avulsion. One hundred and twenty-six teachers completed the first questionnaire, 2 months before the seminar. One hundred teachers completed the second questionnaire 10 months after the seminar. Of these, 70 attended the seminar and 30 did not. Thirty-two teachers who attended the seminar had completed both questionnaires. The percentage of teachers who provided expected 'correct' answers in the first questionnaire (11% and 16%) was significantly lower than that in the second questionnaire (23% and 68%). The percentage of teachers who provided correct answers in the second questionnaire among those who attended the seminar (24% and 69%) was not significantly different from those who did not attend the seminar (20% and 66%). An educational campaign in the community with a seminar targeted towards a cohort of physical education teachers can improve the knowledge of the teachers, even those who did not attend the seminar, probably by means of a contamination effect. Despite the improvement, which was found, the level of knowledge after the campaign remained low and more public health promotion efforts are indicated.

Chi-Square Distribution↗

In vitro viability, mitogenicity and clonogenic capacity of periodontal ligament cells after storage in four media at room temperature.

The choice of storage medium for preserving traumatically avulsed teeth is important for the success of future replantation. The objective of this study was to compare the effectiveness of four recommended storage media (Hank's balanced salt solution [HBSS], culture medium, alpha minimal essential medium [alpha-MEM], and ViaSpan) to preserve cultured periodontal ligament fibroblasts (PDLF) at room temperature (22 degrees C). PDLF were obtained from explants of extracted healthy human teeth. Plates with confluent PDLF were soaked in the various media for 2, 8 and 24 h at room temperature. A control group was incubated with culture medium at 37 degrees C. After incubation, viability of the cells was determined by trypan blue exclusion test. Viable cells were then analyzed for mitogenic (with thymidine) and clonogenic capacity (by culturing one cell/well). Viability of PDLF stored up to 24 h was comparable in all tested media, and the differences were limited to 1%-3%. PDLF stored for up to 24 h in various media had statistically comparable mitogenicity to the control group. After 8 h of storage, the differences were limited to 2%-9%, except for the alpha-MEM group which had 23%-29% lower mitogenic capacity compared to the control group. Increasing the storage time up to 24 h further decreased the mitogenicity of the cells by 22%-47%. The highest mitogenicity after 24 h of storage was found in PDLF stored in culture medium or HBSS, and the lowest in alpha-MEM. PDLF stored for 2-8 h in various media had a comparable clonogenic capacity to the control group. However, after 24 h, the cells' clonogenic ability dropped by 14%-66%. A similar trend of reduction was noted in the mitogenic and clonogenic capacity, although it was statistically significant only in the clonogenic capacity. Culture medium and ViaSpan, followed by HBSS, were the most effective in preserving the clonogenic capacity of PDLF after 24 h of storage. The lowest clonogenic capacity after 24 h of storage was in the alpha-MEM group (66%, P < 0.0025). In conclusion, culture medium, followed by HBSS and ViaSpan, was the most effective media for preserving the viability, mitogenicity and clonogenic capacity of PDLF stored for up to 24 h at room temperature. The lowest functional abilities were found in PDLF stored in alpha-MEM.

Adenosine↗

Tooth dislocation: the relationship with tooth wear and dental abscesses.

Tooth dislocation (tilting) was recorded in 1,200 skulls from 34 museum collections. The findings of dislocation by tooth type, tooth wear, and abscess location are presented. A model for dislocation based upon the progressive loss of tooth support provides a rational explanation for the phenomenon. Physiological continuous tooth eruption was considered to account for a component of the progressive loss of tooth attachment. The process of attrition, pulp perforation, and dental abscess cavity formation resulted in further, more severe loss of tooth support. Heavy functional forces, in association with greatly reduced bone support, tilted the crown lingually and root buccally. When the tooth had tilted to such an extent that the root apices protruded from the bone and, presumably (in life) through the gingival/mucosal tissues, the infected root canals were effectively isolated from the internal environment. The tooth continued to function. The more typical consequence of severe attrition and dental abscess formation was tooth loss; it also isolated an infected tooth from living tissue, but without the benefit of retaining function.

Bicuspid↗

Effect of splinting on the mechanical and histological properties of the healing periodontal ligament in the vervet monkey (Cercopithecus aethiops).

Healing of the periodontal ligament (PDL) after extrusive luxation of two upper central incisors was evaluated when one tooth was splinted and the other left untreated. One millimetre thick, transverse sections of tooth, PDL and alveolar bone were examined in a materials testing machine. Load deformation curves were recorded and a number of mechanical properties were assessed. To eliminate the influence of differences in sizes and fibre arrangements, load values were reduced by the area and deformation values by the width of the PDL, and comparisons between splinted and non-splinted teeth were made at identical root levels 2 weeks after injury. Healing was also evaluated histologically at 1, 2, 3, 4 and 8 weeks after injury. There were no significant differences in mechanical and histological properties between splinted and non-splinted teeth, which suggests that splinting is of doubtful value in treatment of extrusive luxated teeth. The values for the mechanical properties of injured PDL had returned to 50-60% of those of uninjured PDL by 2 weeks after injury, indicating a rapid healing rate.

Animals↗

Prosthodontic rehabilitation of the partially edentulous trauma patient by using osseointegrated implants.

Osseointegrated dental implants provide a viable alternative of tooth replacement. Although certain patients may greatly benefit from this method of treatment, implants are not a panacea. Success is the culmination of good case selection, thoughtful treatment planning, meticulous clinical and technical attention to detail, and proper maintenance care.

Dental Implantation, Endosseous↗

Retained tooth vitality after 15 years' displacement into floor of mouth. Report of a case.

A case in which a tooth traumatically displaced into the floor of the mouth retained its vitality for 15 years is reported. Although the presence of the tooth and soft-tissue cuff which appeared to represent the original attached gingiva presented a source for complications, no significant pathologic changes were noted. The requirement for a thorough radiographic examination before definitive treatment of maxillofacial injuries is emphasized.

Adult↗

Nasotracheal tube obstruction from a central incisor. Report of a case.

After maxillofacial trauma, teeth and other objects can become potential foreign bodies. Whenever possible, careful examination before intubation should be undertaken to avoid serious morbidity secondary to aspiration. The anesthesiologist should be informed and necessary preventive measures taken. This article describes a situation in which this not being done could potentially have led to serious complications.

Adult↗

Tooth in cheek.

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Adolescent↗

The lost tooth.

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Adolescent↗