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At least 1,243 records · Page 69Linked to original sources
PubMed2000

[Fibronectin in adhesion, spreading and proliferation of mandibular condylar cartilage cells on cytodex-3 microcarrier].

OBJECTIVE: The purpose of this study was to investigate the effects of exogenous fibronectin on the attachment and growth of mandibular condylar cartilage (MCC) cells on DEAE-dextran micro-carrier. METHODS: MCC cells were harvested from newborn New Zealand white rabbits by sequential digestion with trypsin and collagenase. They were grown on the 20 mg/L fibronectin-coated and uncoated cytodex-3 micro-carrier. Samples were collected after 1, 3, 5, 7, 9, 11 days. The kinetics of adhesion and growth were observed with phase contrast microscope and environmental scanning electronic microscope (ESEM) and quantified by 0.1% crystal violet nuclear extrusion method. RESULTS: MCC cells could rapidly attach and spread onto the fibronectin-coated cytodex-3, and nearly 60 percent cells attached to the micro-carrier within 2 hours. Spreading cells were flat and lowly refractile observed under phase contrast microscope. While the attachment and spreading of the cells in the untreated group were slow, and only 36 percent cells attached to the uncoated micro-carrier. However, most cells attached to the micro-carrier in both groups after 24 hours. The MCC cells attaching to FN-coated cytodex-3 observed under ESEM were flatter and adhered tightly with more processes (pseudopodia) stretching out from cytoplasm. In contrast, cells on untreated cytodex-3 remained regularly round even 24 hours after being plated. Meanwhile, there was an accelerated growth rate of the MCC cells on the fibronectin treated cytodex-3. The cell density in the FN treated group was higher than that of the untreated group at the end of the culture. CONCLUSION: Fibronectin, one major glycoprotein of extracellular matrix, plays an important role in cell attachment and spreading. The better understanding of the extracellular matrix will be helpful in selecting the optimal substrate for cell growth in vitro.

Adhesiveness↗
PubMed1977

Inhibition of surface immunoglobulin centeral capping of Daudi cells and cell spreading of HeLa-S3 cells by neocarzinostatin.

The effects of an antitumor antibiotic, neocarzinostatin (NCS), on the surface immunoglobulin central capping induced by anti-immunoglobulin M antibody on Daudi cells and on the cell spreading of trypsinized HeLa-S3 cells were examined. Pretreatment of Daudi cells and HeLa-S3 cells with NCS, 5 to 30 micrograms/ml, for 4 hr inhibited cap formation and cell spreading, respectively. It was shown that there is a direct relationship between the dose and the degree of inhibition. Inhibitors of DNA synthesis such as bleomycin, mitomycin C, and 1-beta-D-arabinofuranosylcytosine showed no inhibitory effect on cap formation or cell spreading. However, known microtubule-acting agents such as colchicine and vinblastine sulfate completely inhibited both capping and cell spreading at a dose of 10 micrograms/ml. In view of the fact that 10 micorograms NCS per ml also inhibit the formation of microtubular paracrystals induced by vinblastine sulfate in HeLa-S3 cells and that other agents known to influence microtubule function such as local anesthetics and calcium ionophores also inhibit both paracrystal formation and cap formation, these new observations add further support to our hypothesis that NCS affects microtubular proteins transmembranously in vivo.

Anesthetics, Local↗
PubMedDate not supplied

How many millimeters do atypical epithelia of the pancreas spread intraductally before beginning to infiltrate?

BACKGROUND/AIMS: The pathogenesis of ordinary duct cell carcinoma of the pancreas is still unclear. The aim of this study was to identify which epithelial lesion was a precursor of duct cell carcinoma and to determine how many millimeters atypical epithelia spread intraductally before beginning to infiltrate. METHODOLOGY: Small cystic dilated lesions were studied, using 1374 autopsied pancreata which were mainly taken from aged patients. Serial sections were taken at about 5-mm intervals. Small cystic dilated lesions were defined as restricted dilatations of the pancreatic branch duct, which were grossly recognizable and therefore larger than about 1-2 mm in diameter. The degree of epithelial atypia of the cystic dilated lesions was histologically divided into five groups (Group I to V). RESULTS: Comparing PanIN to our classification, normal and squamous metaplasias were almost the same as Group 1. PanIN 1A and 1B almost the same as Group 2 (benign hyperplasia). PanIN-2 is almost the same as Group 3, and PanIN-3 is almost the same as Group 4, which is characterized by true cribriforming, and a loss of nuclear polarity. Of 1374 autopsy cases, small cystic dilated lesions were found in 378 pancreata (27.5%). Histological examination revealed that the incidence of each group was 47%, 33%, 18%, 2.4% and 0.6% of 507 cystic dilated lesions, respectively. Dilatation of the cystic lesion and surrounding dilated duct resembled a precursor of IPMT in four cases, but not in another five cases. Group IV epithelia just infiltrated in some of the latter cases. Invasion was found when Group IV epithelia spread about 5-8 mm, while it rarely infiltrated when it spread less than 4 mm. CONCLUSIONS: The pathogenesis of ordinary duct cell carcinoma of the pancreas was speculated to begin with papillary growth of duct epithelia. Papillary atypical epithelia then begins to infiltrate when they spread intraductally about 5-8 mm.

Adult↗
PubMed2004

Perineural spread of malignant melanoma of the head and neck: clinical and imaging features.

BACKGROUND AND PURPOSE: Extension of malignant melanoma along cranial nerves is a little-known complication of malignant melanoma of the head and neck. We describe the clinical and MR imaging findings of perineural spread of malignant melanoma to cranial nerves, emphasizing that this entity occurs more commonly with desmoplastic histology and may have a long latent period following primary diagnosis. METHODS: At two institutions, we identified and retrospectively reviewed eight cases of malignant melanoma of the head and neck that had MR imaging evidence of perineural spread of disease. All patients underwent confirmatory tissue sampling. RESULTS: Seven patients had melanomas of the facial skin or lip, and one patient had a primary sinonasal lesion. By histopathology, these melanomas included five desmoplastic, two mucosal, and one poorly differentiated melanotic spindle-cell tumor. All patients developed symptomatic cranial neuropathy an average of 4.9 years from the time of initial diagnosis. MR imaging demonstrated postgadolinium enhancement of at least one branch of the trigeminal nerve in all cases and of at least one other cranial nerve in five cases. Other findings included abnormal contrast enhancement and soft tissue thickening in the cavernous sinus, Meckel's cave, and/or the cisternal segment of the trigeminal nerve. CONCLUSION: Although perineural spread of disease occurs most commonly with squamous cell carcinoma and adenoid cystic carcinoma, malignant melanoma must also be included in this differential diagnosis, particularly if the patient's pathology is known to be desmoplastic. Similarly, any patient with malignant melanoma of the head and neck who undergoes MR imaging should receive an imaging assessment focused on the likely routes of perineural spread.

Adult↗
PubMed2004

[Morphology of epidermoid carcinoma A431 cells spread on immobilized ligands].

Cell interaction with extracellular matrix is a multi-step process characterized by cell attachment to substrata with subsequent cell spreading accompanied by actin cytoskeleton and cellular membrane receptor reorganization. It has been shown elsewhere that epidermoid carcinoma A431 cells, spread on solid substrata coated with fibronectin, laminin-2/4 or antibodies to EGF receptor, form specific actin filament structures typical for each particular ligand. Here quantitative analysis of heterogeneous A431 cell population spread on the above ligands has been reported. Cells were subdivided into morphological classes, according to their shape and actin filament structure, and the relationship among classes under various experimental conditions were quantitatively estimated for every ligand. We studied the influence of cell detachment pattern, short-term and long-term starvation, and cell incubation in suspended state in the medium before plating on the cell population composition. It was possible to recognize the modal morphological class of cells with typical actin cytoskeleton structure dominating for the ligand in the population. Long-term starvation and incubation in suspension before cell spreading are considered as the crucial experimental parameters leading to dramatic changes in cell population.

Actin Cytoskeleton↗
PubMed2004

Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.

CD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.

Animals↗
PubMed2004

Airborne spread of disease--the implications for dentistry.

The potential for the airborne spread of disease has been recognized for many years. Recent studies have shown that this mode for disease transmission is capable of spreading a fatal disease such as Severe Acute Respiratory Syndrome over a wide area. Many dental procedures produce extensive aerosols and splatter that are routinely contaminated with bacteria, viruses, and blood. In the past, the potential for these aerosols and splatter to be a vector for disease spread has not been emphasized in dental infection control. Recently published data shows a need to reassess the potential for dental aerosols and splatter to spread disease and the need for their control. Simple and inexpensive methods for the control of dental aerosols and splatter are given. Dental personnel are urged to make the control of aerosols a standard part of their infection control procedures.

Air Pollution, Indoor↗
PubMed2004

Detection of inter-spread repeat sequence in genomic DNA sequence.

Various types of periodic patterns in nucleotide sequences are known to be very abundant in a genomic DNA sequence, and to play important biological roles such as gene expression, genome structural stabilization, and recombination. We present a new method, named "STEPSTONE", to find a specific periodic pattern of repeat sequence, inter-spread repeat, in which the tandem repeats of the conserved and the not-conserved regions appear periodically. In our method, at first, the data on periods of short repeat sequences found in a target sequence are stored as a hash data, and then are selected by application of an auto-correlation test in time series analysis. Among the statistically selected sequences, the inter-spread repeats are obtained by usual alignment procedures through two steps. To test the performance of our method, we examined the inter-spread repeats in Mycobacterium tuberculosis and Zamia paucijuga genomic sequences. As a result, our method exactly detected the repeats in the two sequences, being useful for identifying systematically the inter-spread repeats in DNA sequence.

Algorithms↗
PubMed2005

[Extracapsular spread as an ominous prognostic sign in laryngeal cancer].

OBJECTIVE: Impact of extracapsular spread upon survival and recurrence rates in patients with laryngeal cancer. MATERIAL AND METHODS: Retrospective analysis of 309 patients with pathologically confirmed regional metastases with and without extracapsular spread (144 and 165 patients respectively). To examine impact of clinical variables upon treatment outcomes we used logistic regression (logit) and Kaplan-Meier method to calculate 5-years disease free survival. RESULTS: Extracapsular spread significantly reduced 5-years survival (40% vs 77%; p = 0.005) and increased regional recurrence rates (33% vs 12%; p = 0.0001). CONCLUSION: Authors suggest use of radiotherapy after neck dissection in presence of extracapsular spread.

Disease-Free Survival↗
PubMed2005

[Effects of enamel matrix proteins on the attachment,spreading and proliferation of porcine bone marrow stromal cells in vitro].

PURPOSE: The purpose of this study is to investigate the influence of enamel matrix proteins(EMPs) on the attachment, spreading and proliferation of cultured porcine bone marrow stromal cells (BMSCs). METHODS: BMSCs were obtained from porcine bone marrow aspiration and cultured in DMEM medium with 10% FBS.The third passage cells were exposed to various concentrations of EMPs (25,50,100 and 200microg /ml).Controls were BMSCs cultured in DMEM medium without EMPs. The cell attachment was analyzed by a colorimetric assay and cell spreading rates were performed at culture time of 1h, 3.5h and 6.5h by analysis of micrographs taken at predetermined sites of each wells. BMSCs proliferation rates were carried out over a 9-day period and assessed by an MTT assay. A parametric one-way analysis of variance (ANOVA) and multiple comparisons were used to test the treatments based on the Stument-Newman- Keuls test. RESULTS: It was shown that BMSCs well attached and spread on EMPs-coated substrata,however, there were no significant differences between the control group and experimental groups with various concentrations of EMPs. The proliferation of BMSCs was significantly stimulated by EMPs in a dose- and time- dependent manner,and EMPs at a concentration of 200microg/ml significantly enhanced BMSCs proliferation from day 3 over the experiment. CONCLUSIONS: The results demonstrate that EMPs stimulate BMSCs proliferation, whereas this factor does not affect the attachment and spreading of these cells. These findings lend theoretical support to the combined application of EMPs and BMSCs in repairing periodontal defects.

Animals↗
PubMed1990

Localization of beta 1, beta 3, alpha 5, alpha v, and alpha IIb subunits of the integrin family in spreading human erythroleukemia cells.

The localization of five integrin subunit proteins was studied in human erythroleukemia (HEL) cells spreading on various culture substrata in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) and the absence of serum. The cells readily adhered on fibronectin, but TPA was needed for adherence on vitronectin and for the spreading of the cells on both substrata. Indirect immunofluorescence microscopy showed that in the spread cells cultured on vitronectin or fibronectin for 2 hours, beta 1, beta 3, alpha 5, and alpha IIb integrin subunits were localized at focal adhesions as identified by talin-immunoreactivity. The alpha v integrin immunoreactivity was initially found at the focal adhesions when the cells were cultured on vitronectin, but was also found later in cells cultured on fibronectin. The alpha IIb integrin immunoreactivity disappeared from focal adhesions within 24 hours. The alpha 5 and beta 1 integrin immunoreactivities disappeared from the focal adhesions in cells cultured on vitronectin, but not in cells cultured on fibronectin. When the cells were plated on glass substratum in the presence of TPA, they spread much slower than on vitronectin or fibronectin, but some cells showed focal adhesions after only 8 hours in culture. In this case, the alpha v and beta 3 integrin subunits were found at focal adhesions. After TPA treatment, HEL cells deposited thrombospondin-immunoreactive material onto their culture substratum, but synthesis of fibronectin, vitronectin, fibrinogen, or von Willebrand factor was not detected. Thus, the results suggest that TPA would activate several integrin receptors in HEL cells and also stimulate the secretion of thrombospondin, which might be used as an adhesion ligand for the integrin vitronectin receptor alpha v/beta 3 complex.

Cell Adhesion↗
PubMed1991

Electric measurements can be used to monitor the attachment and spreading of cells in tissue culture.

A new electrical assay to measure the attachment and spreading of cells in tissue culture has been developed and substantiated by comparison with a more conventional assay. Small gold electrodes are vacuum deposited on the bottom of standard polystyrene culture dishes and coated with various proteins. As mammalian fibroblasts attach and spread on these surfaces, the measured impedance of the electrodes changes. These impedance changes reflect the amount of area blocked by the spreading cells. Since the weak electrical signals used have no noticeable effects on the cells, this is a very convenient method that is both quantitative and sensitive for measuring cell attachment and spreading.

Animals↗
PubMed1991

Effects of intraocular irrigating solutions on the spreading of rabbit corneal endothelial cells on extracellular matrices.

The effects of four commercially available irrigating solutions on the spreading of rabbit corneal endothelial cells on various extracellular matrices were studied. Cultured rabbit corneal endothelial cells, suspended in one of the following intraocular irrigating solutions, Opeguard MA, BSS, BSS Plus, lactated Ringer solution (Lactec) or physiological saline, were placed on uncoated tissue culture plates or on plates coated with extracellular matrices (fibronectin, laminin, collagen type I, or collagen type IV). The cell area was measured after 45 minutes' incubation. The cells spread on all of the extracellular matrices examined but not on the uncoated tissue culture plates. On the fibronectin or laminin matrix, the cell area was significantly greater with Opeguard MA or BSS Plus. On laminin and collagen type IV, the cell area was the greatest with Opeguard MA. On collagen type I, the cell area was significantly greater with Opeguard MA, BSS, or BSS Plus. These results demonstrated that the rabbit corneal endothelial cells responded to the extracellular matrices, and that Opeguard MA or BSS Plus provided more favorable conditions for the spreading of these cells. These results indicated that both Opeguard MA and BSS Plus might aid the spreading of corneal endothelial cells during wound-healing immediately after intraocular surgery.

Animals↗
PubMedDate not supplied

A prospective study on reproducibility of the spread of spinal anesthesia using plain 0.5% bupivacaine.

This prospective study was carried out to determine whether a spinal block with plain 0.5% bupivacaine spreads similarly when repeated in the same patient. During a 14-month period, 50 patients underwent from two to five spinal anesthesias using identical anesthetic technique, including the same premedication, the same volume of injected plain 0.5% bupivacaine, the same site of injection and the same position of the patient. Plain 0.5% bupivacaine was injected with the patient lying in the lateral horizontal position. Thereafter, the patients were turned to the supine horizontal position. Pinprick analgesia was recorded 60 minutes after the injection. Regression analysis showed that the reproducibility of the maximal analgesic segmental spread of the second anesthesia on the basis of the initial anesthesia was highly significant (p less than 0.0001). The blocking results of the patients who underwent from three to five anesthesias within the study period were in accordance with the spread of the first anesthesia. If a higher or lower spread of anesthesia is desirable, a modification or another local anesthetic for the spinal anesthesia may be preferred.

Adult↗
PubMed1979

Tumor cell aggregation and mode of cancer spread in linitis plastica type of gastric carcinoma.

Spread of tumor cells in linitis plastica type of gastric carcinoma was studied in relation to features of tumor cell aggregation which were classified into two types: free-cell type and small nest type. Free-cell type was accompanied by a larger amount of fibrous stroma in submucosal layers than in small nest type. In the gastric wall lymphatics and regional lymph nodes, small nest types was more prominent than the free-cell type. Lymphatic permeation was noted predominantly in the submucosa. In the mucosa, both free-cell and small nest types were frequently present. In carcinoma with free-cell type predominance, diffuse infiltration with infrequent lymphogenous spread was noted, while in carcinoma with small nest type predominance, spread via the lymphogenous route was observed more frequently. The mode of cancer spread in this type of carcinoma appeared to be affected by the type of tumor cell aggregation dominant in the mucosa.

Adenocarcinoma, Scirrhous↗
PubMed1990

Type I and type IV collagen promote adherence and spreading of human type II pneumocytes in vitro.

Human lung tissue was enzymatically digested with dispase and type II pneumocytes were subsequently separated on a discontinuous metrizamide gradient. In primary adherence assays with freshly isolated cells, mean adherence values of 21.7, 19.6, 13.4, 6.8 and 7.0% were obtained after 48 hours on type I collagen (CI), type IV collagen (CIV), fibronectin (FN), laminin (LM) and tissue culture plastic, respectively. Secondly, readherence and spreading assays were performed with type II pneumocytes, previously cultured on CI, FN, matrix of murine EHS cells, and culture plastic. After a culture period of 48 to 72 hours, the cells were replated and tested on CI, CIV, FN, LM, CI + FN, CI + LM, CIV + FN and CIV + LM substrata in order to find out whether preculture substrata can modulate the adherence response of these cells. Readherence values, 1 hour after replating, were 3.3- to 8.6-fold higher than primary adherence values after 48 hours. The highest readherence values were always obtained on collagen-containing substrata after preculture on fibronectin. Spreading values after replating ranged between 35.4 and 6.6% on collagen-containing substrata, the highest values were again obtained after preculture on FN. Less than 6.9% and 0.5% of the cells spread on pure FN and pure LM, respectively. The data of the present investigation indicate that human type II pneumocytes adhere and spread preferentially on collagenous substrata rather than on other components of the extracellular matrix. This might be important in helping us to understand the functional in vivo activity of these cells, especially as regards re-epithelialization of the injured pulmonary alveolus.

Cell Adhesion↗
PubMed1986

[Spinal anesthesia with hyperbaric tetracaine and bupivacaine: velocity of spread, analgesic effect and motor blockade in various positions and injection volumes].

The effect of Trendelenburg position (5 degrees head-down) for 60 or 45 s on the spread of hyperbaric 0.5% tetracaine and 0.5% bupivacaine (3 or 4 ml) was studied in 80 patients scheduled for urological surgery. The mean spread of analgesia was faster (p less than 0.05) and greater (p less than 0.05) in patients tilted head-down for 45 s receiving 4 ml tetracaine than in those with 3 ml tetracaine and 60 s Trendelenburg position. With bupivacaine corresponding results of both volumes, 3 or 4 ml, were similar. Tetracaine compared with bupivacaine showed a faster cephalad spread with 4 ml (p less than 0.05) but not with 3 ml. The onset time of motor blockade in all groups was short (less than 10 min) and complete (Bromage III). The results prove that 4 ml of 0.5% tetracaine with 5% Trendelenburg position for 45 s ensure a rapid and safe spread of analgesia with adequate motor blockade for transurethral surgery.

Aged↗
PubMed1988

[Platelet spreading in diabetes mellitus].

In 162 test persons divided into healthy control persons and diabetics of type I and type II the thrombocyte spreading was investigated according to the method of Breddin. Age, sex, degree of seriousness of retinopathy, duration of diseases, present level of blood sugar and HbA1 concentration were taken into account. Spread thrombocyte forms were increasingly found in old age, in diabetics of both types and a close relation to the extent of retinopathy was evident. As diabetic retinopathy became evident and with growing degree of seriousness, spread forms of thrombocytes were increasingly found, so that the increased spreading capacity may be interpreted as a disturbed metabolic and blood vessel situation in diabetes mellitus.

Adolescent↗