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At least 1,243 records · Page 69Linked to original sources
PubMed1983

Single cell protein as an occupational hazard.

Single cell protein (SCP) intended for animal feed purposes was produced in a pilot plant. The SCP consisted of Methylomonas methanolica, a pseudomonas species which is an obligate methanol user. The SCP was cultured in fermenters and later dewatered and dried in a spray-drier. Seven of eight research workers had febrile reactions 6-12 hours after exposure to SCP dust. All workers had high titres of IgG and IgM antibodies against the pseudomonas species as measured with indirect ELISA and passive haemagglutination techniques. The mechanism behind the febrile reaction is judged to be a non-immunological reaction caused by endotoxins. By increasing the particle size of the SCP through using different drying procedures, a product which generated less dust was obtained.

Animal Feed↗
PubMed1981

The effect of topical povidone iodine on wound infection following abdominal surgery.

The effect of povidone iodine on wound sepsis following gastrointestinal surgery was studied in a trial involving 153 patients of whom 72 had their wounds sprayed with povidone iodine dry powder (Disadine DP) and 81 acted as a control group. The infection rate of 9.9 per cent in the patients treated with povidone iodine was significantly lower than that of 24.4 per cent in the control group (P less than 0.05). Bacterial contamination of the wound at the time of operation was shown to be of importance, being associated with a 52 per cent infection rate in the control group. However, spraying of contaminated wounds with povidone iodine reduced the infection rate to the significantly lower level of 11 per cent (P less than 0.05). We conclude that povidone iodine is a safe and effective means of reducing wound sepsis following gastrointestinal surgery.

Administration, Topical↗
PubMed2005

The effect of different adhesives on vinyl polysiloxane bond strength to two tray materials.

STATEMENT OF PROBLEM: Although stock trays often provide mechanical retention for elastomeric impression materials, manufacturers typically recommend the use of an adhesive, whether a stock or custom tray is used. Universal adhesives for vinyl polysiloxane (VPS) are used in practice, but comparative bond strengths have not been reported. PURPOSE: The aim of this study was to evaluate the bond of 3 VPS materials with a methylmethacrylate autopolymerizing and a light-polymerizing tray material, using the adhesive recommended by the manufacturer of the impression material, and 2 universal adhesives (paint-on and spray-on). MATERIAL AND METHODS: Ten specimens per group, for a total of 180 flat 15 x 15 x 20-mm specimens, were made from the 2 tray materials and finished to 320 grit with silicon carbide paper. Four paint-on adhesives (Coltene, Caulk, Kerr VPS, or universal VPS) and 1 spray-on adhesive (Sili Spray) were applied and dried following manufacturers' instructions or for 10 minutes. Three impression materials, Affinis, Aquasil, and Take I, were automixed and injected into a perforated cylinder positioned in a universal testing machine. Tray specimens were positioned against the open cylinder end in contact with the VPS material. Tensile tests (cross-head speed 5 mm/min) were conducted until adhesive separation failure. Mean values and standard errors of the adhesive strength were recorded in MPa for each material combination. Data were first analyzed with Welch analysis of variance (ANOVA) to determine overall significance, and then with a 3-way ANOVA and the Tukey HSD test to make pairwise comparisons, with alpha=.05 for all testing. RESULTS: For all impression materials tested, the universal spray-on adhesive consistently demonstrated significantly lower bond strengths than all other adhesives (P<.05). Equivalent or significantly (P<.05) higher bond strength values were found for the universal paint-on adhesive for the 3 impression materials tested. CONCLUSION: The use of GC paint-on universal adhesive provided significantly higher adhesive values than those obtained with the adhesives supplied by the manufacturers of the impression materials tested, with the exception of the Kerr impression and adhesive material combination where no significant differences were found.

Adhesives↗
PubMed2004

Evaluation of inoculation method and inoculum drying time for their effects on survival and efficiency of recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes inoculated on the surface of tomatoes.

A study was undertaken to evaluate methods for applying inoculum and to examine the effect of inoculum drying time on survival and recovery of foodborne pathogens inoculated onto the surface of raw, ripe tomatoes. Five-strain mixtures of Escherichia coli O157:H7, Salmonella, or Listeria monocytogenes were applied to tomatoes by dip, spot, or spray inoculation methods. Inocula were dried for 1 or 24 h at 22 degrees C before tomatoes were treated with water (control) or chlorine (200 micrograms/ml). Significantly (alpha = 0.05) larger populations (CFU per tomato) of E. coli O157:H7 and Salmonella were recovered from dipinoculated tomatoes than from spot- or spray-inoculated tomatoes. This difference was attributed to larger numbers of cells adhering to tomatoes subjected to dip inoculation. Populations of E. coli O157:H7 and Salmonella recovered from spot- and spray-inoculated tomatoes containing the same initial number of cells were not significantly different. Significantly different L. monocytogenes population sizes were recovered from inoculated tomatoes (dip > spot > spray). Populations of pathogens recovered from tomatoes were significantly larger when inocula were dried for 1 h compared with 24 h. Significant differences (water > chlorine) were observed in the sizes of populations for all pathogens recovered from tomatoes treated with chlorine, regardless of inoculation method or drying time. Results indicate that inoculation method, drying time, and treatment affect survival and/or recovery of foodborne pathogens inoculated onto the surface of tomatoes. We recommend that spot inoculation with a drying time of 24 h at 22 degrees C be used with standard methods to determine the efficacy of chlorine and other sanitizers for killing foodborne pathogens on tomatoes.

Bacterial Adhesion↗
PubMed2003

[Diagnosis and therapy of myofascial trigger points].

AIM: Myofascial trigger points (MTrPs) are hyperirritable tender spots in palpable tense bands of skeletal muscle. Muscle is an orphan organ, no medical specialty claims muscle as its organ. The article aims at filling some of the gaps in the current knowledge of MTrPs. METHODS: The presented findings were partly obtained in experiments on anesthetised rabbits, partly they are the result of ample experience with patients suffering from MTrPs. DIAGNOSIS: Each muscle has a characteristic elicited referred pain pattern that, for active MTrPs, is familiar to the patient. Without a laboratory test or imaging method, diagnosis of MTrPs depends entirely on history and physical examination. MTrP symptoms follow muscle overload, are activated acutely by sudden overload, or develop gradually with prolonged contractions or repetitive activity. The diagnostic skill required depends on considerable innate palpation ability, authoritative training, and extensive clinical experience. THERAPY: Effective treatment methods include manual stretching by trigger-point pressure release, contract-relax, vapo coolant spray-and-stretch, and dry needling or injection of MTrPs. CONCLUSIONS: The integrated hypothesis presents an explanation for the pathophysiology of MTrPs and begins with excessive release of acetylcholine from involved motor endplates. It depends on a new understanding of the abnormality of endplate noise. Biopsies demonstrate segmental shortening of groups of sarcomeres in individual muscle fibres and possibly waves of contracted sarcomeres to account for palpable taut bands.

Animals↗
PubMed1993

Walking behaviour and population density of adult linyphiid spiders in relation to minimizing the plot size in short term pesticide studies with pyrethroid insecticides.

Temporal changes in spatial distribution and population density of linyphiid spiders were studied in relation to physical factors and deltamethrin spraying in the field. The results of the study have been used to set the conditions necessary when plot size is reduced in field trials. The results showed independence of observations at 7 m from an unsprayed reservoir, with respect to migration, in the week following pesticide application. Soil moisture had a positive effect on trapping success, without having apparent effect on trapping success, without having was found for deltamethrin spraying on wet and dry soil. Shifts in the spatial distribution of spider activity showed a close correlation with crop microclimatic conditions. The effect of trapping on population density was shown to be limited. The results suggest that small plots surrounded by a border of 7 m are appropriate for assessing short term pesticide effects on adult linyphiid spiders in winter wheat.

Journal Article↗
PubMed2006

Nano- and micro-particulate formulations of poorly water-soluble drugs by using a novel optimized technique.

A novel technique for the production of nano- and micro-particulate formulations of poorly water-soluble drugs has been developed. This technique involves the use of static mixer elements to provide fast precipitation by continuous turbulent mixing of two liquid flows, an aqueous phase and an organic phase, respectively. The objective of this study was to develop the mixer technique by investigating the influence of the element number on the particle size of the resulting dispersions. Four model active pharmaceutical ingredients (APIs) with a variety of polymers, lipids or surfactants underwent intensive mixing and the final suspensions showed a narrow size distribution. Parameters such as the flow rate and the temperature of the precipitated organic-aqueous phases were also significant in the reduction of particle size. Further development of the mixing technique led to reproducible and stable formulations with minimal excipient amounts. These formulations were spray- or freeze-dried to improve stability.

Cryoelectron Microscopy↗
PubMedDate not supplied

Myofascial pain syndrome and trigger-point management.

BACKGROUND AND OBJECTIVES: Myofascial pain syndrome (MPS) is a common condition often resulting in referral to a pain clinic. The epidemiology, pathogenesis, and various diagnostic tools are reviewed, and a variety of treatment methods are discussed. METHODS: Extensive periodical literature and textbooks are reviewed, and selected manuscripts are critically analyzed. RESULTS: The incidence of MPS with associated trigger points appears to vary between 30 and 85% of people presenting to pain clinics, and the condition is more prevalent in women than in men. Patients complain of regional persistent pain, ranging in intensity and most frequently found in the head, neck, shoulders, extremities, and low back. Muscle histologic abnormalities have been described in some studies. Similarly, electromyographic, thermographic, and pressure algometric studies have inconsistently identified abnormalities. A multidisciplinary approach to treatment appears to be most beneficial and may include such modalities as trigger-point injections, dry needling, stretch and spray, and transcutaneous electrical nerve stimulation. CONCLUSIONS: The definitive pathogenesis of MPS is currently unknown, and no single diagnostic method is consistently positive. While trigger-point injection is the most widely employed method of treatment, other modes of therapy have also proved to be effective.

Adult↗
PubMed2002

Povidone-iodine spray technique versus traditional scrub-paint technique for preoperative abdominal wall preparation.

OBJECTIVE: The study was conducted to compare povidone-iodine spray and traditional scrub-paint techniques in reducing abdominal wall bacteria during preoperative preparation. STUDY DESIGN: Sixty patients scheduled to undergo vaginal surgery were recruited for study. Cultures of the abdominal skin were performed before and after preparation with two techniques: A traditional 5-minute iodophor soap scrub-paint on one half and povidone-iodine aqueous spray on the other. Multiple pairwise comparisons were performed with the Wilcoxon signed rank test. A P value of.05 was considered statistically significant in all analyses. RESULTS: The mean number of colonies for spray after 1 minute was 1.83 +/- 3.16, for spray after 3 minutes was 0.40 +/- 1.15, and after 5-minute scrub was 0.87 +/- 2.97. Both techniques, the spray after 3 minutes and the 5-minute scrub, were statistically more effective at reducing bacterial counts than the spray after 1 minute. There was no statistically significant difference between the spray after 3 minutes and the scrub techniques. CONCLUSION: Povidone-iodine applied as a spray and left to dry for 3 minutes appears as effective as the traditional scrub-paint technique in reducing abdominal wall bacteria before abdominal surgery.

Abdomen↗
PubMed2006

Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118.

Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.

Anti-Bacterial Agents↗
PubMed1976

Detection of sulfonamides in animal feeds.

The animal feed sample is extracted with ethanol or acetone and the extract is evaporated to dryness. Another portion of the same sample, spiked at the 3 ppm level with 8 sulfonamides, is similarly extracted and the extract is evaporated to dryness. The residue from each solution is dispersed with 5 ml 0.1N NaOH and, following the addition of 1 ml 1N HCl and mixing, the solution is filtered. The filtrate is mixed with Celite, transferred to a column, and eluted with ammoniacal ether. Aliquots of the concentrated sample and control eluates are spotted on a neutral Adsorbosil-1 thin layer chromatographic (TLC) plate. Following development in chloroform-methanol (95+5) and drying, the plate is sprayed with an alcoholic solution of p-dimethylaminobenzaldehyde until the control chromatogram shows 6 yellow spots which are, from top to bottom; sulfadimethoxine (SO), the combined sulfamerazine (SM)-sulfamethazine (SH)-sulfaquinoxoline (SQ) spot, sulfadiazine (SD), sulfapyridine (SP), sulfathiazole (SZ), and sulfaguanidine (SG). A spot on the sample chromatogram can be identified if the Rf is identical to one of the 5 sulfonamides not overlapping with another compound. If the Rf of the sample spot is approximately the same as that of the combined SM-SH-SQ spot, more definite identification can be obtained by using basic Adsorbosil-1 TLC plates, with chloroform-methanol (92+8) as the developing solvent.

Adsorption↗
PubMedDate not supplied

[Procedure for isolating nisin from Streptococcus lactis].

The paper describes a method for obtaining the antibiotic nisine from Streptococcus lactis str. MSU via 50% acetic acid extraction. The concentrate is dried in the Anhydro spray drier (Denmark). The preparation is inactivated during a one-year storage in the refrigerator by 20-25%.

Drug Stability↗
PubMed1977

A rapid mini-thin-layer chromatographic procedure for the detection of barbiturates and common sedatives at overdose levels.

A simple, rapid thin-layer chromatographic method is presented for the detection of barbiturates in blood, serum, or urine in overdose situations. Extraction with ether is followed by evaporation and reconstitution with methanol. Part of the extract is spotted on a 2.5 multiplied by 7.5 cm thin-layer chromatographic plate which is then developed in a mixed solvent system. After drying, the plate is sprayed with n, 2, 6-trichloro-p-benzoquinone imine and heated. The presence of a barbiturate is indicated by a blue spot. Chromatography in another solvent system assists in the identification of the particular barbiturate ingested.

Barbiturates↗
PubMed1976

Collaborative study of accelerated development of latent fingerprint images on paper by application of steam.

Nine latent fingerprint examiners participated in a collaborative study in the use of a steam iron to accelerate chemical development of latent images on bond and newsprint papers treated with a 0.5% solution of ninhydrin in acetone. Four latent images were placed on each paper by the Associate Referee during an 8 hr period. The papers were paired and numbered 1, 2, and 3. Three pairs were mailed to each collaborator who treated Pair 1 by dipping, Pair 2 by brushing or swabbing, and Pair 3 by spraying. After air-drying, the papers were subjected to heat and steam from a household steam iron until the images developed. The average time for developing all images was 5.8 min/paper. Of the total images available, 92.1% were developed to some degree; 47.9% of these images or 43.5% of all images were identified by the collaborators. The method has been adopted as offical first action.

Dermatoglyphics↗
PubMed1996

The effect of inhaled and intranasal sodium cromoglycate on symptoms of upper respiratory tract infections.

BACKGROUND: A well established drug for the treatment of asthma and allergy, sodium cromoglycate, was found in open trials to be useful as a symptomatic treatment for upper respiratory tract infections. OBJECTIVE: To compare the efficacy of inhaled and intranasal sodium cromoglycate and matching placebos on the symptoms of upper respiratory tract infections. METHODS: Adult subjects with symptoms of runny nose, throat pain, or cough for less than 24 h were recruited. They were treated for 7 days using a randomized, double-blind, placebo-controlled, group comparative design. The medication given was: sodium cromoglycate dry powder 20 mg per inhalation in spincaps; sodium cromoglycate aqueous nasal spray delivering 5.2 mg per dose; or matching placebo as dry powder and nasal spray. One spincap and one spray per nostril were taken every 2 h during waking hours on days 1 and 2 and then four times daily on days 3-7. Severity of nine symptoms (general malaise, body aches and pains, chills and shivering, sneezing, nasal running, nasal blocking, sore throat, cough and voice disturbance) was recorded twice daily by subjects on diary cards, using a scale of 0 (absent) to 3 (severe). RESULTS: The study was conducted between February and April 1993. One hundred and eighteen patients aged 21-63 years (mean 41 years) were included. Symptoms resolved faster (P < 0.001) and the severity in the last three days of treatment was significantly less in patients treated with sodium cromoglycate than with placebo (P < 0.05-day 5; P < 0.01-day 6; P < 0.001-day 7). Side effects were local and mild and did not differ between the treatment groups. CONCLUSION: Sodium cromoglycate administered both by inhalation and intranasally is an effective treatment for the symptoms of upper respiratory tract infection. Its combined safety and efficacy would make it an acceptable form of treatment for these conditions.

Administration, Inhalation↗
PubMed2003

Effects of annealing lyophilized and spray-lyophilized formulations of recombinant human interferon-gamma.

The purpose of this study was to examine the effects of adsorption of recombinant human interferon-gamma (rhIFN-gamma) on ice surfaces and subsequent drying during processing by spray-lyophilization and lyophilization. Ice/liquid interfacial areas were manipulated by the freezing method as well as by the addition of an annealing step during lyophilization; that is, rhIFN-gamma adsorption was modified by the addition of nonionic surfactants. rhIFN-gamma was lyophilized or spray-lyophilized at a concentration of 1 mg/mL in 5% sucrose, 5% hydroxyethyl starch (HES) +/- 0.03% polysorbate 20 in 140 mM KCl, and 10 mM potassium phosphate, pH 7.5. After the samples were frozen, half were annealed on the lyophilizer shelf. Recovery of soluble protein was measured at intermediate points during processing. On drying, the secondary structure of rhIFN-gamma was determined by second-derivative infrared (IR) spectroscopy, specific surface areas (SSAs) were measured, scanning electron micrographs (SEM) were taken, and dissolution times were recorded. Adsorption of rhIFN-gamma to ice/liquid interfaces alone was not responsible for aggregation. Rather, drying was necessary to cause aggregation in lyophilized sucrose formulations. Addition of an annealing step to the lyophilization cycle resulted in more native-like secondary protein structure in the dried solid, eliminated cracking of the dried cakes, and suppressed both the formation of air/liquid interfaces and rhIFN-gamma aggregation on reconstitution.

Adsorption↗
PubMed1995

Rapid monitoring of soil, water, and air dusts by direct large-area alpha spectrometry.

During retrieval and disposition of wastes containing transuranium elements, continuous monitoring of the air, water, and soil for alpha emitters was required to ensure that safety limits were not exceeded and that the waste itself was not disturbed unknowingly. Direct measurements by alpha spectrometry were particularly promising because of their potential speed, sensitivity, and their ability to identify transuranium radionuclides under field conditions. Soil samples or settled dusts were finely ground, suspended in 80% ethanol, sprayed onto circular stainless steel pans, and dried on a hotplate. Water samples were mounted directly by spraying. Air dusts were collected with a high-volume air sampler on 20- by 25-cm membrane filters. The samples were then analyzed directly in a large pressurized gridded ionization chamber without further sample preparation. The lower limits of detection for 10-min counting times were 1.5 Bq g-1 (40 pCi g-1) for 100-mg soil samples, and 4 x 10(-2) Bq m-3 (10(-12) microCi mL-1) for a 10-min air sample taken at 0.4 m3 min-1 (14 cubic feet per minute) and counted without waiting for decay of radon progeny.

Air Pollutants, Radioactive↗
PubMed1993

An evaluation, using computerized image analysis, of antimicrobial efficacy of an automatic hand washing machine with ultrasonic wave spraying.

We studied the antimicrobial efficacy of hand washing with a quick-dry hand washing machine [TE-KIREIKI] employing ultrasonic wave spraying and its incidental alcohol-based product [AROKULIN-E]. The subjects of this study were 10 males who had been instructed not to use any antimicrobial agent for the previous 2 weeks or any hand soap for the previous 5 hours. They pressed their palms on agar before and after washing their hands. After 48-hour incubation at 37 degrees C, the bacterial colonies grown on the agar were counted using the [ASPECT] image processing system. The colony count was expressed as the post-stamp versus pre-stamp percent (%) reduction. Two patterns of hand washing were examined in this study: [A] a 3-second hand wash using an alcohol-based product [AROKULIN-E] and [B] a 30-second hand wash using a nonmedicated detergent soap with running water in addition to hand washing by [A]. The percent (%) reduction after hand washing patterns [A] and [B] were 49.1% and 51.3%. These reduction rates indicated that these patterns did not eradicate bacteria from the hand surface. Therefore, we concluded that this quick-dry hand washing machine employing an ultrasonic wave spraying method combined with an alcohol-based product needs improvement.

Bacteria↗