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Use of pneumocystoplasty for overinflation of the swim bladder in a goldfish.

A Ryukin goldfish was evaluated because of a 6-month history of progressive abdominal distention and positive buoyancy. Overinflation of the swim bladder was diagnosed, and the fish was anesthetized with tricaine methanesulfonate. Archimedes' principle was used to determine the volume of swim bladder that was removed surgically. The caudal swim bladder was exteriorized through an abdominal incision and 2 surgical clips were placed across it to limit its size. After surgery, the fish remained in a state of negative buoyancy in sternal and lateral recumbency on the bottom of the tank. Sutures were removed 15 days after surgery, but the fish died 24 days after surgery. A full necropsy could not be performed because of autolysis of the tissues, but the surgical clips and the swim bladder appeared unremarkable. Pneumocystoplasty may be a viable treatment for this condition.

Air Sacs↗

Sperm swimming velocity as evaluated by frame lapse videography and computer analysis.

A videographic method is described to measure the swimming velocity of fresh human, frozen-thawed bull and white mussel (Donax serra) sperm. The method can be accurately controlled. The pattern of sperm movement can be analyzed on a frame-by-frame basis or over 0.0206-sec intervals. Sperm velocities can be standardized per time unit. Sperm swimming velocity of different species ranging from very slow to very fast erratically moving sperm can be measured. Parameters such as swimming speed, progressive swimming speed, progressiveness ratio, and percentage motility can be evaluated.

Animals↗

Levels of reactive oxygen species before and after sperm preparation: comparison of swim-up and L4 filtration.

In previous studies, high levels of reactive oxygen species (ROS) have been associated with an adverse impact on the fertilizing ability of sperm. The present study evaluated ROS levels in unprocessed (raw) semen specimens and in specimens processed by the traditional swim-up method and by L4 filtration, thereby assessing the potential of these techniques to damage sperm. Semen specimens from 26 men (10 patients in whom subfertility was suspected and 16 donors with normal fertility) were randomly collected, and ROS levels were measured. For all specimens combined (those from patients and those from donors), ROS levels were significantly lower after L4 filtration (6.07 +/- 1.97 mV/s 10(9) sperm-1, p < .01) and after swim-up (5.70 +/- 0.96 mV/s 10(9) sperm-1, p < .001) than in unprocessed ejaculate (12.88 +/- 2.32 mV/s 10(9) sperm-1). However, ROS levels in swim-up and L4 specimens were not statistically different from each other. It would appear that sperm processing by either swim-up or L4 filtration yields specimens with significantly lower levels of ROS than are found in unprocessed ejaculate.

Cell Separation↗

Combination of direct swim-up technique and discontinuous Percoll gradient centrifugation for sperm preparation of oligoasthenozoospermic samples.

Sperm recovery for assisted reproduction in oligoasthenozoospermic patients is not satisfying either by the swim-up technique or by Percoll gradient centrifugation, and no single technique is constantly preferred. The design of this study was to evaluate the effects of combining the two methods on improving the efficacy of sperm preparation in these poor samples. For each semen sample, 1 mL was treated with a combination method, which used direct swim-up technique to recover motile sperm swimming to the supernatant, and then the residual semen was subjected to two-layer discontinuous Percoll gradient procedure for further recovery of motile sperm. Another 1 mL was prepared with two-layer discontinuous Percoll gradient centrifugation alone for comparison. Parameters measured included sperm concentration, number of progressively motile sperm, percentage of progressive motility, percentage of motile sperm recovery, amount of debris, percentage of normal forms according to Kruger's strict criteria, and motion characteristics of sperm using computer-aided motility analysis. The results of 30 oligoasthenozoospermic samples demonstrated that the combination method achieved a significantly greater recovery of motile sperm than the two-layer discontinuous Percoll gradient centrifugation did (43.2 +/- 19.7% vs. 32.2 +/- 14.3%, p < .05). The percentage of progressive motility was higher in the samples of the combination method than in those of Percoll gradient centrifugation alone, but the difference was not significant (63.7 +/- 21.8% vs. 58.7 +/- 20.1%). The debris of semen was removed equally well by both methods. The percentage of normal forms as well as motion characteristics, including curvilinear velocity, straight-line velocity, mean amplitude of lateral head displacement, and linearity, were similar in the samples treated by these two procedures. The combination of the direct swim-up technique and discontinuous Percoll gradient centrifugation combines the advantages of each procedure and results in greater recovery of motile sperm in oligoasthenozoospermic samples.

Cell Separation↗

Deformation of the notochord by pressure from the swim bladder may cause malformation of the vertebral column in cultured Atlantic cod Gadus morhua larvae: a case study.

This study describes a malformation that frequently occurs in Atlantic cod Gadus morhua in intensive culture systems. The malformation is characterised by a slight upward tilt of the head and an indented dorsal body contour at the transition between the head and the trunk, and is first evident to the fish farmer when the cod reach the juvenile stage. These abnormalities are associated with malformations of the neurocranium, the cranial region of the vertebral column and the cranial part of the epaxial lateral muscles. The pathogenesis involves deformation of the notochord, which can be observed in larvae about 7 d post-hatch (dph) and onwards. The deformation consists of an increase in dorsal curvature of the notochord in the region above the swim bladder. In the same region, the notochord has an abnormal cross-sectional outline, characterised by a groove-shaped, longitudinal impression along the ventral surface of the sheath. In most cases, the swim bladder fills the impression, and in severely affected larvae it forms a hernia-like lesion in the notochord. The deformation of the notochord seems to be conveyed to the vertebral body anlagen (chordacentra), which in teleosts are formed by mineralisation within the notochordal sheath. The vertebral bodies adopt an abnormal wedge shape, with a ventral concavity, and the neural arches are most often S-shaped. A continuous range of degrees of the malformation can be observed. All these pathomorphological characteristics are compatible with the notion that the notochord has been subjected to an upward mechanical force, probably generated by a persistent increase in pressure between the swim bladder and the notochord during the period of development of the vertebral anlagen. Our results thus indicate that the critical time window with regard to development of the malformation is from 18 to 36 dph, when the initial formation of the vertebrae takes place. Chronic overinflation of the swim bladder or pathological dilatation of the digestive tract may cause the lesions, and aetiology may be related to factors that influence the function of these organs.

Age Factors↗

Flow cytometric comparison between swim-up and Percoll gradient techniques for the separation of frozen-thawed human spermatozoa.

Viable human spermatozoa were recovered from cryopreservation by either swim-up or Percoll density gradient techniques and their cell-surface oligosaccharide components were compared to those of fresh sperm. Sperm were labeled with FITC-Con A and analyzed by fluorescence microscopy and flow cytometry. By fluorescence microscopy, fresh sperm were uniformly labeled in the neck region alone. Frozen-thawed sperm, obtained by either swim-up or Percoll density gradients, showed two patterns of staining: one sperm population was stained in the neck region only, whilst another showed staining in both the neck and acrosome regions. No difference between sperm recovered by the two techniques was apparent. Flow cytometry profiles of fresh sperm revealed a single peak of fluorescence, whereas cryopreserved sperm gave two peaks of fluorescence. Samples recovered by Percoll density gradient contained significantly more sperm with the same fluorescence pattern as that observed for fresh sperm than did those recovered by swim-up. Cryopreservation alters the cell-surface oligosaccharide components of spermatozoa. Recovery of cryopreserved sperm by Percoll density gradient yields a greater proportion of sperm which resemble fresh sperm than does swim-up.

Cell Separation↗

[Identification of two new genes involved in swimming motility of Pseudomonas aeruginosa].

Pseudomonas aeruginosa, a typical opportunistic pathogen, has a single polar flagella which provides swimming motility and virulence. Twelve of insertional mutants with Mu transposition complexes into genomic DNA of Pseudomonas aeruginosa were screened on the changes of swimming motility. After gene cloning and DNA sequencing of those mutants, ten of them were illustrated that defective mutations on swimming motility were caused by insertions of Mu transposon into 10 of different functional genes which may involved in biosynthesis of flagella and gene regulations, but another two mutants were resulted from the insertion of two new genes (PA2950, PA5022) with function unknown. Observation of these two mutants with an integrity flagellum under electron microscope showed that two new genes were not involved in construction of flagella, but probably in energy metabolism and gene regulation of swimming motility.

Blotting, Southwestern↗

The effect of exhaustive swimming and chloramphenicol on the content of mitochondrial protein and cytochromes in rat liver and myocardium.

The content of mitochondrial protein and cytochromes aa3, b, c1 and c in rat liver and heart was determined in four groups of rats: controls, CAP-treated rats, exercised rats and CAP-treated exercised rats. Single swimming does not change the amount of mitochondrial protein in liver and myocardium as well as the content of cytochromes aa3, b, c1 and c in liver mitochondria. The content of cyt. c is lowered in heart mitochondria. CAP treatment of rats for three days decreases the amount of mitochondrial proteins and cyt. aa3, and b whereas no such changes are observed in heart mitochondria. The subsequent swimming on the background of the inhibitor does not affect additionally liver mitochondria, but lowers significantly the mitochondrial protein and the content of cyt. aa3, b, c1 and c in the myocardium. These data show that exhaustive muscular work alters the effect of CAP in heart mitochondria. Three hours of exhaustive swimming are not sufficient to change neither the amount of total mitochondrial protein, nor the cytochrome content. The lowered cyt. c content in heart mitochondria after swimming is explained by an intensified permeability of the outer membrane. Thus, the changes in the activity of some enzymes and the oxidative phosphorylation previously reported by us, are not due to alterations in the protein synthesis.

Animals↗

Computer assisted measurement in normal and pathological human semen, fresh and post swim-up technique.

A total of 429 semen samples were studied. Two hundred and twenty-nine samples with normal characteristics were processed following a basic procedure; the remaining 200 were normal and pathological samples that were analyzed pre and post swim-up. Semen specimens were allowed to liquefy for 30 minutes and sperm count, motility, velocity and linearity were determined using the Cellsoft Automatic Semen Analyzer. In normal patients, a significant increase of motility, velocity and linearity (p less than 0.001) post swim-up, was observed. Sperm recovery in this group was 13.9 +/- 1% of the whole motile sperm population. In the polyzoospermic group, recovery of motile spermatozoa post swim-up was significantly decreased as compared with the normal group (p less than 0.001). In the asthenozoospermic group (plus either hypospermia or hyperspermia, or with more than 30% of motile spermatozoa or less than 30% of motility), no variations of velocity or linearity as compared to the normal group were observed. In all the pathological groups studied, a significant increase in velocity and linearity (p less than 0.001) post swim-up, was observed.

Computers↗

Bidirectional swimming in spermatozoa of Tephritid flies.

Our observations show that spermatozoa of the Mediterranean fruit fly Ceratitis capitata and of Dacus oleae and Dacus dorsalis are capable of swimming backwards as well as forwards, and that they can change direction abruptly. The preferred direction is backwards, observed in spermatozoa obtained from the male genitalia. Forwards swimming spermatozoa were frequently seen in the spermatheca and close to the eggs. The change in swimming direction appears to be effected solely by a change in the direction of bend propagation, with no significant change in other waveform parameters. In vitro reactivated spermatozoa swim forwards only and require a minimum free Ca++ concentration of about 10(-6) M for movement. A switching of wave propagation from one direction to the other under control of intracellular free Ca++ concentration is suggested. Perhaps the backwards movement allows easier delivery of spermatozoa from the common envelope embedding the heads in the male apparatus, and assures a more efficient movement of the sperm towards the egg, especially given the enormous relative length of the head. The forwards movement is favoured in order to orient the sperm for penetration of the micropile.

Animals↗

A field study of some swimming-pool waters with regard to bacteria, available chlorine and redox potential.

The waters of one indoor bath and three outdoor baths were examined once an hour during 3 days (bath 1) or 6 days, for available chlorine, redox potential, permanganate number, ammonium, nitrate and total nitrogen, total bacterial count at 22 degrees C., total bacterial count at 37 degrees C. and faecal coliform bacteria. The weather, number of swimmers and the chlorine gas addition were continuously registered, and the pH was checked a few times at each bath. In bath 1, an indoor pool with aluminium sulphate precipitation about once a week and with sand filters back-washed every 2 days, less than 10 bacteria/ml. were found in all samples. In bath 2, an outdoor pool with aluminium sulphate precipitation twice a week and with sand filters back-washed twice a week, also few bacteria were found. In bath 3, an outdoor pool with only filtering through sand filters back-washed about every 14 days, high bacterial counts were found every day except the first, when the filters had been newly back-washed. In bath 4, an outdoor pool with only filtering through sand filters back-washed about once a week, high bacterial counts were found now and then during the first 4 days when the weather was warm, but few bacteria were found the last 2 days when the weather was cold and windy, and there were few swimmers.Values from different analyses on the same sample showed relatively good correlation between the redox potential and the free available chlorine. In bath 3 both the redox potential and the available chlorine were weakly correlated to the bacterial count, but in bath 4 there was no such correlation. No other factors were well correlated with the bacterial count either.The bacterial counts at 22 degrees and 37 degrees C. were of the same order. No faecal coliforms were ever found. Use of these bacteria as indicator organism in swimming pools is criticized.The method of using certain minimum values of the free available chlorine as guarantee for a satisfactory bacteriological quality of the swimming pool water is also questioned. The degree of purity of the water is fundamentally connected with the disinfecting power of the available chlorine.Use of certain minimum values of the redox potential, according to these investigations, seems to be a method of somewhat greater accuracy. Provided that the methods of precipitation are performed correctly and filters are being back-washed often enough, then an automatically registering redox potential device, perhaps connected to the chlorine gas pump, ought to constitute a good control of the hygienic quality of a swimming-pool water. This must, however, always be completed by bacteriological examinations, preferably made at high bathing load.

Bacteria↗

A statewide outbreak of cryptosporidiosis in New South Wales associated with swimming at public pools.

From December 1997 to April 1998, 1060 laboratory-confirmed cryptosporidiosis cases were reported in New South Wales, Australia. In a case-control study, compared with 200 controls, the 100 cases were younger (mean age 42 versus 71 years; P < 0.0001), more likely to report swimming at a public pool (59% versus 38%; adjusted OR and 95% CI = 27; 1.4-5.1) and swimming in a dam, river or lake (OR = 48; 1.1-20.3) but less likely to report drinking bottled water (OR = 0.4; 0.2-0.9). In subgroup analyses, in rural areas illness was associated mainly with contact with another person with diarrhoea, and in urban areas illness was associated with swimming in a public pool. Cryptosporidium oocysts were more commonly detected in pools to which at least two notified cases had swum (P = 004). Outbreaks of cryptosporidiosis can be prolonged, involve multiple pools and be difficult to control.

Adolescent↗

Evaluation of the health risk associated with exposure to chloroform in indoor swimming pools.

The exposure of swimmers to chloroform (CHCl3) was investigated in indoor swimming pools of the Quebec City region along with the associated carcinogenic risk. Six training sessions involving 52 competition swimmers (11 to 20 yr old) were conducted in 3 different pools, while 12 adult leisure swimmers attended 5 sessions, each held in a different pool. For each session, water and ambient air CHCl3 concentrations were measured and CHCl3 levels in alveolar air samples (CHCl3 ALV) collected from swimmers prior to entering the swimming pool premises and after 15, 35, and 60 min of swimming. Mean water concentrations varied from 18 microg/L to 80 microg/L, while those in air ranged from 78 microg/m3 to 329 microg/m3. Multiple linear regression analyses revealed that CHCl3 ALV values in competition swimmers were strongly correlated to ambient air and water levels, and to a lesser degree to the intensity of training. Only ambient air concentration was positively correlated to CHCl3 ALV in the leisure group. Concentrations of CHCl3 metabolites bound to hepatic and renal macromolecules, estimated using a physiologically based pharmacokinetic (PBPK) model, were 1.6 and 1.9 times higher for the competition swimmers than for the leisure swimmers, respectively. The highest hepatic concentration predicted in competition swimmers, 0.22 microg CHCl3 equivalents/kg of tissue, was at least 10,000 times lower than the smallest no observed effect level for liver tumors in animals. Data indicate that the safety margin is therefore very large, for competitive swimmers as well as for leisure swimmers.

Adolescent↗

Reducing illness transmission from disinfected recreational water venues: swimming, diarrhea and the emergence of a new public health concern.

Recreational water-related illnesses are associated with swimming in contaminated water venues. The transmission of diarrheal illness in disinfected settings is influenced by several factors which include: chlorine resistance in waterborne pathogens; poor facility maintenance of disinfectant levels; and lack of healthy swimming habits. Health care providers can help to disseminate healthy swimming messages to their patients and help to prevent and control this emerging public health concern.

Adolescent↗

Pulmonary epithelial integrity in children: relationship to ambient ozone exposure and swimming pool attendance.

Airway irritants such as ozone are known to impair lung function and induce airway inflammation. Clara cell protein (CC16) is a small anti-inflammatory protein secreted by the nonciliated bronchiolar Clara cells. CC16 in serum has been proposed as a noninvasive and sensitive marker of lung epithelial injury. In this study, we used lung function and serum CC16 concentration to examine the pulmonary responses to ambient O3 exposure and swimming pool attendance. The measurements were made on 57 children 10-11 years of age before and after outdoor exercise for 2 hr. Individual O3 exposure was estimated as the total exposure dose between 0700 hr until the second blood sample was obtained (mean O3 concentration/m3 times symbol hours). The maximal 1-hr value was 118 microg/m3 (59 ppb), and the individual exposure dose ranged between 352 and 914 microg/m3hr. These O3 levels did not cause any significant changes in mean serum CC16 concentrations before or after outdoor exercise, nor was any decrease in lung function detected. However, children who regularly visited chlorinated indoor swimming pools had significantly lower CC16 levels in serum than did nonswimming children both before and after exercise (respectively, 57 +/- 2.4 and 53 +/- 1.7 microg/L vs. 8.2 +/- 2.8 and 8.0 +/- 2.6 microg/L; p < 0.002). These results indicate that repeated exposure to chlorination by-products in the air of indoor swimming pools has adverse effects on the Clara cell function in children. A possible relation between such damage to Clara cells and pulmonary morbidity (e.g., asthma) should be further investigated.

Air Pollutants↗

[Is Dutch swimming pool water erosive?].

Etiological factors in the development of dental erosion are usually listed as dietary acids, for instance in soft drinks and fruit juices, and intrinsic acid exposure due to gastro-intestinal disease or frequent vomiting. Quite often the list of causes in reviews and textbooks also includes frequent swimming. This paper evaluates the evidence behind this erosion etiology. The main disinfection techniques using gas chlorination and sodium hypochlorite are described, and their relative risk for development of low pH water is discussed. In the Netherlands only the relatively safe sodium hypochlorite method is used, and the quality of the water in public swimming pools is monitored monthly by independent test laboratories. Data for 2001 from such a test laboratory show that the percentage of low-pH results is very low (0.14%). It is concluded that the risk of dental erosion from frequent swimming in acidic pool water is probably negligible in the Netherlands.

Humans↗

[Incidence of otitis externa following swimming pool attendance--a case study in 6 indoor pools].

A bacteriological study of the microflora in the auditory meatus of patients with otitis externa after swimming in correlation to the water flora was done during winter 80/81. Six indoor-pools (monthly controls ahead had proven unobjectionable water quality) were analyzed daily, while all swimmers were asked to come for otologic examination whenever symptoms of otitis externa appeared. In a control group without otitis externa the auditory canal was inspected and swabs were taken immediately after swimming. The results indicate, that a short term contamination of the ear canal is possible by swimming in water of unobjectionable quality; this however does not necessarily lead to clinical appearances. Since the incidence of otitis externa in our study (0.01%) was not higher than the morbidity rate in nonswimmers, the data support the concept of the multiplex etiology for otitis externa.

Bacteria↗

Improved preparation and swimming behavior of Triton-extracted models of Didinium nasutum.

An improved method for the preparation of Triton X-100 extracted models of Didinium nasutum was established. Didinium models prepared by treatment with a Triton X-100 solution, containing specific proteolysis inhibitors and dimethyl sulfoxide, maintained an intact shape at 25 degrees C for a longer time than models prepared by treatment with a Triton X-100 solution not containing the proteolysis inhibitors and dimethyl sulfoxide. The improved Didinium models were reactivated so as to swim forward in the presence of Mg2+ and ATP or ADP. They did not swim backward in response to Ca2+, in contrast to well-known Paramecium models. However, the improved Didinium models showed circular swimming and spinning in response to 10(-6) M or higher concentrations of Ca2+. This observation suggests that the quick directional change as well as the spinning, which are characteristic of live Didinium, are due to an increase in the endoplasmic Ca2+ concentration around the ciliary system. The response of the Didinium ciliary system to Ca2+ may differ from the response of the Paramecium ciliary system to Ca2+.

Adenosine Triphosphate↗