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At least 1,243 records · Page 69Linked to original sources
PubMed1982

Casein synthesis by mouse polysomes and their messenger ribonucleic acid extracts.

Mammary epithelial cells from lactating mice were used to compare the in vitro casein synthesis by membrane-bound and free polysomes with that of messenger ribonucleic acid extracted from free- and bound polysomes (polysomal system and a rabbit reticulocyte lysate system). Casein synthesis was detected by incorporation of hydrogen-3 labeled amino acids in the reaction mixture into synthesized casein molecules. Membrane-bound polysomes synthesized considerably more casein than free polysomes; this difference was abolished by deoxycholate treatment of free polysomes. The synthesizing activity of messenger ribonucleic acid was similar whether extracted from free- or membrane-bound polysomes. Casein synthesis may be controlled by factors on the polysomal membrane.

Animals↗
PubMed1962

Ribonucleic acid in a "membrane" fraction of Escherichia coli and its relation to cell-wall synthesis.

Suit, Joan C. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston). Ribonucleic acid in a "membrane" fraction of Escherichia coli and its relation to cell-wall synthesis. J. Bacteriol. 84:1061-1070. 1962.-A small amount of ribonucleic acid (RNA) was found in a "membrane" fraction prepared from osmotically sensitized Escherichia coli. It exhibited an elevated metabolic activity in that it attained the highest specific activity of any RNA in subcellular fractions of logarithmic-phase cells or spheroplasts prepared from logarithmic-phase cells which had been allowed to incorporate P(32) briefly. The metabolic activity of this RNA, in terms of P(32) incorporation, was found to be independent of cell-wall synthesis in the diaminopimelic acid (DAP)-less mutant, E. coli W 173-25, but was inhibited by penicillin in both this strain and in E. coli B. The latter effect is considered to be a result of other complex inhibitions of cellular metabolism by the antibiotic. The development of sensitivity to osmotic shock, capability of recovery, and synthesis of macromolecules in penicillin-treated and DAP-starved cultures, under these conditions, is described.

Cytoplasm↗
PubMed1971

Thermal denaturation in acidic solutions of double-helical ribonucleic acid from virus-like particles found in Penicillium chrysogenum. A spectrophotometric study.

1. Two species of double-helical RNA isolated from mycelium of Penicillium chrysogenum were titrated with acid at 25 degrees C and 95 degrees C (solvent 0.1m-sodium phosphate buffer). At 25 degrees C denaturation occurred at about pH3. At 95 degrees C in the denatured form cytosine residues titrated as a simple monobasic acid of pK3.9 compared with pK approximately 2.5 for the native form at 25 degrees C. 2. On thermal denaturation in neutral and acidic solutions one species of RNA (38% rG.rC) ;melted' in three distinct stages, equivalent to a mixture of three species, namely one of about 25% rG.rC, another of about 33% rG.rC and a third of about 46% rG.rC: the relative proportions were 0.25:0.35:0.40. 3. On thermal denaturation in acidic solutions the increase in the fraction of ionized cytosine residues concomitant with the ;melting' of rG.rC base pair also affects the spectrum especially at 280nm and serves to enhance the contribution of rG.rC base pairs at this wavelength. The increment in epsilon((P)) at 280nm on ;melting' an rG.rC base pair approaches 53501.mol(-1).cm(-1) depending on pH, compared with 33501.mol(-1).cm(-1) at pH7. In contrast epsilon((P)) at 280nm is scarcely affected by ;melting' rA.rU base pairs or by the protonization of adenine residues. 4. Changes in the spectrum of Escherichia coli rRNA on denaturation in acidic solutions were studied to yield the mole fractions of rA.rU and rG.rC base pairs ;melting' at particular pH values.

Acids↗
PubMed1974

Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels.

Double-stranded nucleic acids from a strain of Penicillium chrysogenum containing RNA viruses were isolated by agarose-gel filtration, and separated into DNA and double-stranded RNA fractions by agarose-gel chromatography in 2.5m-NaCl. The DNA fraction contained less than 1% alkali-labile polynucleotides, and sedimented homogeneously at 8-10S in alkaline sucrose gradients. In CsCl gradients it tended to band in the density region of 1.66-1.72g/ml. It had a ;melting' temperature (T(m)) of 75 degrees C in 0.015m-NaCl-0.0015m-trisodium citrate, corresponding to 51.5mol% of G+C. The double-stranded RNA fraction did not contain detectable DNA. It could not band in CsCl up to a density of 1.78g/ml, and mainly consisted of a 14-15S RNA species with a T(m) of 88.5 degrees C in the above solvent, and a G+C content of 49.3 mol%.

Centrifugation, Density Gradient↗
PubMed2006

RNA recognition and recognition control of alpha-peptide ribonucleic acids containing arginine residue by external factors.

A novel nucleic acid model using alpha-peptide ribonucleic acid (alpha-PRNA) possessing alternative alpha-PRNA/lysine and alpha-PRNA/arginine sequences, where the lysine or arginine residue are expected to stabilize the complex not only by the conventional hydrogen-bonding interactions between the complementary nucleobase pairs but also through the electrostatic interactions between basic amino acid side-chain groups and DNA's phosphate anions on the backbone. These alpha-PRNAs form stable sequence-specific complex with the complementary DNA.

Arginine↗