A geriatric evaluation unit's use of a microcomputer for patient care and research.
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Four computer programs are described which allow two amino acid or DNA sequences to be compared for homology, the results being displayed in a 2-dimensional array on a printer page. The programs also may be used to visualize repeated sequences or dyad symmetry within a DNA sequence. Two of the four programs may be used with any printer, the other two require a printer with graphics capability. Many options are available including using only a portion of a sequence, specifying a window to demonstrate more significant structures and special restrictions on matching such as excluding the third base in a codon. Written in the C programming language, the programs run under the CP/M 80 operating system, and may be copied in binary format through a modem. They are also available for the IBM/PC.
The SEQALIGN programs1 described in this report aid in the assembly of up to 100 individual overlapping DNA sequences generated by M-13 subcloning and sequencing methods. The program produces a printout of the aligned sequences presented in register. Use of the program will be facilitated because 1) it is written with the Microsoft BASIC interpreter, 2) sequence data may be entered and edited using WORDSTAR or similar word processing programs, and 3) hardware requirements for execution of the program on CP/M or MS-DOS (IBM-PC compatible) systems are minimal.
A database-oriented system (pCP123) is described for the manipulation of recombinant DNA data. This system was developed within the context of an integrated software package with spreadsheet, database, graphing and programming capabilities. The system includes two databases, one of sites and another of regions, coordinately handled by a series of macro-programs operated from four user-define menus. A distinctive feature of the system is the possibility of handling both ends of defined functional or structural regions in situations of simulated deletions or insertions.
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An improved package of BASIC computer programs designed to facilitate complete analysis of karyotypes is described. The package's many functions include facilities for measurement of chromosomes, analysis of data, pairing of homologues, designation of sex chromosomes and supernumeraries , storage and recall of data, and generation of idiograms and karyograms .
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