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At least 1,243 records · Page 69Linked to original sources
PubMed1987

Effects of ascorbate ions on intracellular fluorescein emission polarization spectra in cancer and normal proliferating cells.

Ascorbate ions induced a polarization peak in the intracellular fluorescein fluorescence polarization spectra of asynchronous populations of a variety of cancer cell types from human and animal biopsy material or in cells grown in vivo and/or in vitro. The appearance of this polarization peak at 510 nm on excitation at 470 nm (P510 peak) indicates the transition of mitochondria from the condensed (active) into orthodox (resting) conformation. In contrast, no effects of ascorbate ions were observed in the polarization spectra of various normal cell lines. This apparent differential response seems to be caused by the effects of ascorbate ions on several steps of the altered energy metabolism in cancer cells. It appeared not to be due to a defective mechanism responsible for the conformational changes in the mitochondria. In YMDR cells resistant to the antitumor drug methylene-dimethanesulphonate (MDMS), HeLa cells pretreated with colcemid, and YMDS cells pretreated with cytochalasin B, the transition of mitochondria into orthodox conformation could not be induced by ascorbate ions. Thus, it is possible that tumors also pretreated with other drugs become resistant to growth inhibitory effects of ascorbate ions. Induction of the fluorescein emission polarization peak at 510 nm in cells from biopsies or from in vitro-induced malignancies could be used as an additional criterion for malignant transformation.

2,4-Dinitrophenol↗
PubMed1979

Immunoassay with fluorescein-labelled antigens--device of a method and its clinical application.

A simple, sensitive, highly reproducible method for assay of antibodies using fluorescein-labelled antigens according to the method by Farr is described. We measured titers of anti-BSA antibodies and antigen binding capacity of sera in BSA immunized rabbits by fluorometric assay with fluorescein isothiocyanate (FITC) labelled BSA, and obtained highly reproducible and quantitative results. The resultd times more sensitive than the results from single radial immunodiffusion. FITC-labelled double or single stranded calf thymus DNA was incubated with sera of patients with systemic lupus erythematosus and New Zealand Black mice. Both anti-double and single stranded DNA antibodies were detected in sera of systemic lupus erythematosus, but only anti-single stranded DNA antibodies in sera of New Zealand Black mice.

Animals↗
PubMed1983

Analysis of Brugia pahangi microfilariae surface carbohydrates: comparison of the binding of a panel of fluoresceinated lectins to mature in vivo-derived and immature in utero-derived microfilariae.

A fluoresceinated lectin binding assay was employed to detect carbohydrates on the sheath and cuticle of mature in vivo-derived, and immature in utero-derived Brugia pahangi microfilariae. The sheath of mature microfilariae bound concanavalin A and wheat germ agglutinin, indicating the presence of N-acetylglucosamine and glucose or mannose. In addition to binding concanavalin A and wheat germ agglutinin, the sheath of in utero-derived microfilariae also bound Limulus polyphemus agglutinin, peanut agglutinin, Ricinus communis agglutinin-I, and soybean agglutinin, indicating the presence of the additional sugars galactose, sialic acid, and N-acetylgalactosamine. There was no evidence of cuticle carbohydrates, as none of the tested fluoresceinated lectins bound to either mature or immature exsheathed microfilariae. The significance of these results in terms of the survival of microfilariae in the mammalian host, and development to third-stage larvae in the mosquito vector, is discussed.

Acetylgalactosamine↗
PubMed1976

Analysis of mononuclear cell surfaces with fluoresceinated staphylococcal protein A complexed with IgG antibody of heat-aggregated gamma-globulin.

Fluorescein-conjugated staphylococcal protein A (SPA) was complexed with either: 1) heat-aggregated IgG, 2) B cell specific antibody, or 3) T cell specific antibody and then used for an immunofluorescent analysis of mononuclear cell surfaces. Cellular Fc receptors failed to recognize the Fc region of aggregated IgG that had been blocked by SPA. Moreover, fluoresceinated SPA that had been complexed either with anti-Fab (B-cell specific) or T cell-specific antisera prevented the nonspecific binding of these reagents to the IgG-Fc receptors on mononuclear cells, thereby permitting the latter to be properly identified as B or T lymphocytes. In addition, when unconjugated SPA was added to presensitized target cells in a test for antibody-dependent cell-mediated cytotoxicity, cytolysis was abrogated.

Animals↗
PubMed1976

[Fluorescein angiographic picture of sympathetic ophthalmia (author's transl)].

The fluorescein angiographic picture of sympathetic ophthalmia is described in an instructive case, which first started at the posterior pole. In the acute phase there was a picture of an exudative process with multiple subretinal enlarging hyperfluorecent spots and some dye-pooling. The retinal vasculature was unaffected. In the healing phase a transitory involvement of the optic nerve head was observed. The cicatricial phase was characterized by a coarsening of the pigment pattern of the fundus. In addition to fluorescein angiography the degree of transitory hyperopia was an excellent indicator of the activity of the disease process.

Fundus Oculi↗
PubMed1991

Detection and quantification of hyperfluorescent leakage by computer analysis of fundus fluorescein angiograms.

Digital imaging systems can be used for direct acquisition of images of the ocular fundus or for their indirect acquisition from fundus photographs or transparencies. Computerised image processing techniques can then be used to manipulate and quantify features of interest. We describe a method for the detection and quantification of macular leakage in fluorescein angiograms. The rate of change in fluorescence over time is examined on a pixel-by-pixel basis and used to provide a gradient threshold that discriminates pixels displaying leakage from normal pixels. A region-growing technique is then used to enhance the detection of leakage missed by gradient thresholding alone. This report discusses the potential applications of the technique and highlights the methodology required to obtain reproducible results.

Fluorescein↗
PubMed1986

Retinal fluorescein leakage in retinitis pigmentosa.

Of 82 consecutive patients with retinitis pigmentosa, 78 underwent fluorescein angiography using standard and special, abbreviated photographic protocols. The stereoscopic angiograms disclosed alterations of the blood-retinal barrier, primarily at the level of the retinal pigment epithelium, in 60 patients. Areas of leakage were bilateral in all but two persons. The macula most frequently showed leakage, followed by the optic disk, the arcade areas, and the periphery. A few individuals exhibited leakage from the perifoveal retinal vessels. None of these patients had significant retinal vascular alterations or exudates in the retinal periphery. In eyes with no cataract or detectable macular pigment change and with comparable amounts of epiretinal membrane, the presence of macular edema was significantly correlated with reduced central visual acuity.

Adolescent↗
PubMed1987

Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.

Goat anti-mouse antibodies conjugated with colloidal 40 nm gold particles (G40) were used as secondary layers to stain human T lymphocytes in an attempt to extend the polarized light epi-illumination microscopic technique to flow cytometric multiparameter analysis. G40-labelled T cells were further stained with phycoerythrin (PE, red)- and fluorescein (FITC, green)-conjugated antibodies with specificity for the same cells. The cell samples were then analysed on a standard flow cytometer equipped with one laser operating at 488 nm. G40-labelled cells were detected in the side scatter (90 degrees) channel and fluorescent cells in the red and green fluorescence channels, respectively. Single labelling of the same T cell subsets with either G40-, FITC- or PE-conjugated antibodies yielded similar results, and cell mixture experiments did not show interference between gold and fluorescence labels. Triple staining experiments with three differently conjugated antibodies showed that subpopulations of cells were labelled in an independent manner with one, two or three antibodies in proportions expected from fluorescence controls. It was, therefore, possible to detect subset, sub-subset and activation markers on individual T cells. Our experiments show that immunogold staining of cell populations can be detected in routine one-laser flow cytometry. Further, the gold label can be combined to give two-color staining with ordinary red and green fluorochrome-conjugated antibodies thereby permitting rapid and precise triple antibody staining of individual cells. This methodology provides a powerful tool for the analysis of cellular antigenic diversity among, e.g., immunocompetent cells.

Antibodies, Monoclonal↗
PubMed1991

Frequency of adverse systemic reactions after fluorescein angiography. Results of a prospective study.

Intravenous fluorescein angiography is a commonly performed and extraordinarily valuable diagnostic procedure. The frequency of adverse reactions after angiography has varied considerably in previous reports. In a prospective study of 2789 angiographic procedures in 2025 patients, the authors found that the percentage of adverse reactions depended strongly on the patient's angiographic history. Overall, adverse reactions followed 4.8% of the angiographic procedures. These reactions included nausea (2.9%), vomiting (1.2%), flushing/itching/hives (0.5%), and other reactions (dyspnea, syncope, excessive sneezing) (0.2%). No cases of anaphylaxis, myocardial infarction, pulmonary edema, or seizures occurred. The percentage of reactions was 1.8% for patients who had had previous angiography without ever having had an adverse reaction. In contrast, the percentage of reactions was 48.6% for patients who had had an adverse reaction to angiography previously.

Fluorescein↗
PubMed1963

REACTION OF LYMPHOCYTES WITH PURIFIED PROTEIN DERIVATIVE CONJUGATED WITH FLUORESCEIN.

Busy coats from the peripheral bloods of persons known to give a positive reaction to purified protein derivative and of those known to be negative were treated with purified protein derivative conjugated with fluorescein isothiocyanate. Lymphocytes from positive reactors took up the labeled antigen in significantly greater numbers than did those from negative reactors. Leukocytes from newborns showed far less affinity for the antigen than leukocytes from adult negative reactors.

Adult↗
PubMed1987

A method for fundus evaluation in children with oral fluorescein.

A procedure is described for the ophthalmoscopic examination of the fundus in children after 1% or 2% oral fluorescein ingestion without sedation or anaesthesia. No major complications or any allergic reactions were observed. In the ophthalmoscopically normal fundi of the five healthy children studied the fluorescence of the nerve head and retinal blood vessels could be visualised at 15 minutes, increasing at 30 minutes, and declining by 60 minutes. The possible problems of the technique are discussed.

Administration, Oral↗
PubMed1964

THE ESTIMATION OF URANIN (FLUORESCEIN SODIUM) IN BLOOD.

The extraction and estimation of uranin (fluorescein sodium) in plasma is described. Heparin-plasma containing uranin is digested with crude ficin and extracted with acid n-pentanol. The supernatant fluid is re-extracted with N NaOH and, after volume adjustment, read against a uranin standard in a fluorimeter with appropriate filters. It appears reliable within 4% at concentrations as low as 10(-8) g./ml.

Blood Chemical Analysis↗
PubMed1994

In situ measurement of fluorescein release by collagen shields in human eyes.

Fluorescein (F) and FITC Dextran (FD; MW 4400) have been used as inert analogs of active drugs to examine the factors controlling the rate of loss of drugs from collagen shields in vivo. The diffusion constants in the shield (120 microns thick) determined from in vitro release experiments were found to be 0.42 x 10(-6) for F and 0.16 x 10(-6) cm2/sec for FD at 34 degrees C and these values correspond to 0.7 and 0.27 min-1 rates of loss for the exponential parts of their release. In the eyes of 6 subjects, the F loaded shields lost the dye at an average rate of 0.016 min-1 initially, increasing to 0.026 min-1 at the end of an hour. Somewhat higher values were noted with FD and were attributed to variations of manufacturing of the shields. Comparing the rates of loss in in vitro and in vivo, it is clear that the latter is not controlled by the rate of diffusion in the shield, but is limited by the rates of tear secretion and flow over the surfaces of the shield. The penetration of F into the anterior chamber from a series of four drops applied 12 min apart was compared with that from a shield soaked in the drop solution to determine the enhancement in the bioavailability. This showed that the shield increased the penetration into the eye by about 16 times over that from a single drop. However, the rate of loss of F from the shield in vivo is only about 8 times slower than that from a drop instilled in the eye.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

The use of oral fluorescein angiography in idiopathic central serous choroidopathy.

A case report, differential diagnosis, and discussion of idiopathic central serous choroidopathy (ICSC) are presented. Procedures, advantages and disadvantages of oral and intravenous fluorescein angiography are discussed. Oral fluorography may be used to reliably diagnose ICSC and other late-staining disease processes.

Administration, Oral↗
PubMed1985

[Changes in plasma ionized calcium levels during fluorescein angiography].

The authors have demonstrated a significant decrease of the plasmatic ionized calcium level in 84 patients during retinal fluorangiography likely due to a chemical bond between calcium and fluorescein. The side effects noticed during the procedure were similar to that quoted in the literature; their frequency, however, was not correlated with the decrease of the plasmatic ionized calcium level, even though the magnitude of the decrease was twice as great in the patients who experienced some trouble as in those who did not. This lack of correlation may be related to the too small patients sample. A greater frequency of side effects has been noticed in patients treated by calcium inhibitors.

Calcium↗
PubMed1983

[International survey on the incidence of severe or fatal complications which may occur during fluorescein angiography].

Serious or fatal accidents during fluorescein angiography were assessed by an international survey involving answers from 260 clinics in 30 different countries in respect of 594, 687 angiographies. Incidence of fatal accidents was one case per 49,557 angiographies, and of serious accidents, one case per 18,020 angiographies. The total number of accidents reported was 45 cases, equal to one case per 13,215 angiographies. Age, general health of the patients, and the experience of the different clinics who perform this examination, seem to influence the occurrence and the seriousness of these accidents.

Adolescent↗
PubMed1996

Peripapillary fluorescein leakage in 11778 Leber's optic neuropathy.

A 17-year-old young man presented with a 2-month sequential visual loss in both eyes. Vision was 20/200 in the right eye and 20/400 in the left eye. Both discs were hyperemic with circumpapillary telangiectasia and nerve fiber swelling. Fluorescein angiography revealed a fuzzy hyperfluorescence due to leakage from dilated capillaries on both discs in the late transit. Molecular mitochondrial DNA study confirmed a homoplasmic 11778 Leber's optic neuropathy.

Adolescent↗