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At least 1,243 records · Page 69Linked to original sources
PubMed1985

Serological investigation on cattle vaccinated with a killed Brucella abortus strain 45/20 adjuvant vaccine.

Serum antibody reactions were monitored for a period of 18 months in 18 heifers which had been vaccinated with Brucella abortus Killed 45/20 adjuvant vaccine, using four standard serological procedures: the Rose Bengal, Serum agglutination, Complement fixation and Coombs antibovine globulin tests. The Rose Bengal and Serum agglutination tests were persistently negative during the experiment. Titres to the complement fixation test rose sharply for only four weeks after the second dose of the vaccine. Thereafter the titres fluctuated to positive levels at unpredictable intervals. One out of 14 heifers tested 12 months after vaccination was strongly positive to the complement fixation test but all the animals were absolutely negative at the end of the experiment. The Coombs test became strongly positive after the second dose of the vaccine for up to 34 weeks after primary vaccination. The animals were negative to the test at the end of the experimental period but some still retained suspicious titres. It was concluded that the Brucella abortus killed 45/20 adjuvant vaccine did not interfer stall with the Rose Bengal and Serum agglutination tests, that the fluctuations of the Complement fixation titres to positive levels at unpredictable intervals were undesirable and that the Coombs test showed little promise as an assay which would be used to differentiate antibody titres due to Brucella abortus Killed 45/20 adjuvant vaccine from those of natural infection. It would appear that the Complement fixation test and the Coombs antibovine globulin test could be safely used 12 and 18 months respectively, after immunization of cattle with the vaccine.

Agglutination Tests↗
PubMed1975

[Examinations of the behaviour of immunoglobulins IgG, IgA, IgM in comparison to the immunoserological reactions in human chronic brucellosis (author's transl)].

The authors determined the levels of the immunoglobulins IgG, IgA and IgM in the serum of 70 patients with chronical brucellosis which was diagnosed on the basis of epidemiological, clinical and laboratory examinations. The Ig-levels were compared with immunoserological reactions of brucellosis as Wright, Coombs, Burnet, complement fixation tests. A significant increase of immunoglobulin G without a positive correlation with the values of immunoserological reactions was found.

Adult↗
PubMed1979

Age related changes in auto-erythrocyte rosettes in the C57BL/6J and NZB/BINJ mice.

The changes in the auto-erythrocyte rosetting thymic and splenic lymphocytes and the induction of autoimmunity was followed with age in C57BL/6J and NZB/BINJ mice. The auto-erythrocyte rosetting cells (auto-RFC) showed shifts in their pattern in both thymus and spleen in C57BL/6J and NZB/BINJ mice. Both strains had approximately the same percentage (approximately 3%) of thymic auto-RFC at 1 month of age. In C57BL/6J mice the rosette population increased to 6.6% by 2 months, declined after 3 months and subsequently increased gradually with age. In contrast, the NZB/BINJ thymic auto-rosettes peaked at 4 months and gradually declined thereafter. Both the NZB/BINJ and C57BL/6J strains were tested for the presence of anti-erythrocyte antibodies by the direct Coombs' agglutination test. The results showed that at 6 and 10 months 50% and 90% of the NZB/BINJ mice were positive for antibodies, respectively, and the thymic and splenic auto-RFC dramatically decreased in numbers. In the C57BL/6J mice during this same period, very low incidence of auto-antibodies was detected by the Coombs' test and auto-RFC increased in numbers.

Aging↗
PubMed1983

Ceftazidime: aspects of efficacy and tolerance.

Clinical trial results have been analysed from 2607 patients (2953 infections, of which 38% were severe) treated with ceftazidime alone and from 466 patients (507 infections - 24% severe) treated with one or more comparative antibiotics. Of 235 staphylococcal infections (all sites) treated with ceftazidime alone 84% cleared and 16% relapsed or failed; of 28 infections treated with other antibiotics, 82% cleared and 18% relapsed or failed. Of 541 pseudomonas infections (all sites) treated with ceftazidime 60% cleared and 40% relapsed or failed; of 76 infections treated with other agents 43% cleared and 57% relapsed or failed. Adverse events were seen in 8.9% of the 2,607 ceftazidime treated and in 8.2% of the 466 patients treated with other agents and included local and gastrointestinal intolerance and hypersensitivity reactions. Changes in laboratory tests included eosinophilia, Coombs' positivity and liver enzyme increases. Superinfection occurred in 2.5% of patients treated with ceftazidime and in 5.2% of patients treated with other antibiotics. Deaths from all causes during or shortly after treatment were reported in 3.3% of the 2607 ceftazidime-treated and in 5.8% of the 466 patients treated with other agents. Factors, including neutropenia, influencing these differences are analysed.

Bacterial Infections↗
PubMed1982

Clinical observations on Babesia gibsoni and Babesia canis infections in dogs.

Thirty-five cases of Babesia gibsoni infection and 11 cases of Babesia canis infection were diagnosed and treated in dogs at Kadena Air Base in Okinawa, Japan, between April 1979 and February 1980. Diagnosis was made by demonstrating the organisms in blood smears and by serologic examination, using an indirect fluorescent antibody test. Serologic findings correlated well with the occurrence of infection in mature dogs, but poorly in young (1- to 3-month-old) dogs. Although these 2 intraerythrocytic parasites were readily distinguishable on Giemsa-stained blood smears and by the indirect fluorescent antibody test, the clinical syndromes were similar. Most dogs were anorectic and depressed and were found to have regenerative anemia. Of 37 dogs tested, 31 were Coomb's test-positive, and most of these became Coombs' test-negative after treatment and disappearance of clinical signs. Specific treatment consisted predominantly of the use of diminazene aceturate. Pentamidine isethionate also used. Although these drugs were effective in halting and reversing the clinical progression of the disease, they usually were ineffective in clearing the blood of B gibsoni organisms, and relapses commonly occurred. Both drugs appeared to be more effective against B canis. It was concluded that some of the several hundred dogs arriving in the United States annually from Okinawa are carriers of B gibsoni, a parasite only recently discovered in North America.

Animals↗
PubMed1984

An investigation of the role of immunologic factors in anemia associated with canine heartworm disease.

Direct Coombs' antiglobulin tests were performed on 80 dogs with patent Dirofilaria immitis infection and 170 dogs negative for microfilaria of D. immitis. Presence or absence of anemia was determined by hematocrit in 55 of the heartworm negative dogs and 68 of the dogs with heartworm disease. Heartworm infected dogs showed a higher incidence (37%) of anemia than noninfected dogs (14.5%). Anemia was most prevalent in two groups of dogs with heartworm infection, one group showing vena caval syndrome (91%) and the other occult dirofilariasis (62.5%). These latter two groups of dogs also showed a significantly higher number of positive Coombs' reactions at 37 degrees C than other dogs with heartworm disease and the noninfected dogs. The number of positive Coombs' reactions at 4 degrees C among the total of 80 dogs with heartworm infection was significantly higher than that for dogs without heartworm disease. However, there was no positive correlation between anemia and the outcome of the Coombs' test at either temperature. These findings do not suggest that immunologic factors play a major role in the pathogenesis of anemia in dogs with heartworm disease.

Anemia, Hemolytic↗