Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “parameter identification”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,225 records · Page 68Linked to original sources

Fast, robust identification of nonlinear physiological systems using an implicit basis expansion.

Because the number of parameters required by a Volterra series grows rapidly with both the length of its memory and the order of its nonlinearity, methods for identifying these models from measurements of input/output data are limited to low-order systems with relatively short memories. To deal with these computational and storage requirements one can either make extensive use of the structure of the Volterra series estimation problem to eliminate redundant storage and computations (e.g., the fast orthogonal algorithm), or apply a basis expansion, such as a Laguerre expansion, which seeks to reduce the number of model parameters, and hence, the size of the estimation problem. The use of an appropriate expansion basis can also decrease the noise sensitivity of the estimates. In this paper, we show how fast orthogonalization techniques can be combined with an expansion onto an arbitrary basis. We further demonstrate that the orthogonalization and expansion may be performed independently of each other. Thus, the results from a single application of the fast orthogonal algorithm can be used to generate multiple basis expansions. Simulations, using a simple nonlinear model of peripheral auditory processing, show the equivalence between the kernels estimated using a direct basis expansion, and those computed using the fast, implicit basis expansion technique which we propose. Running times for this new algorithm are compared to those for existing techniques.

Algorithms↗

Preoperative risk analysis and postoperative mortality of oesophagectomy for resectable oesophageal cancer.

BACKGROUND: Postoperative mortality after oesophagectomy for oesophageal cancer depends largely on the preoperative physiological status of the patient. METHODS: A composite scoring system to predict the risk of oesophagectomy based on objective preoperative parameters was developed based on a retrospective review of patients operated on and evaluated prospectively in two subsequent patient groups. RESULTS: An initial retrospective multivariate analysis of 432 patients who had oesophagectomy identified a compromised general status (P < 0.001) and poor cardiac (P < 0.001), hepatic (P < 0.05) and respiratory (P < 0.05) function as independent predictors of a fatal postoperative course. Based on the relative risk associated with the individual impaired organ functions, a composite risk score was established. A prospective study in 121 patients confirmed that this composite scoring system provides a better identification of high-risk patients than any of the individual parameters. Inclusion of the composite score into the process of patient selection and choice of the procedure resulted in a decrease of postoperative mortality rate from 9.4 per cent (52 of 553) to 1.6 per cent (four of 252) (P < 0.001). CONCLUSION: The risk of death after oesophagectomy for oesophageal cancer can be assessed objectively before surgery and quantified by a composite risk score. This score provides a useful tool for refining the criteria of patient selection for resection or the choice of procedure.

Esophageal Neoplasms↗

Elimination of the initial value parameters when identifying a system close to a Hopf bifurcation.

One of the biggest problems when performing system identification of biological systems is that it is seldom possible to measure more than a small fraction of the total number of variables. If that is the case, the initial state, from where the simulation should start, has to be estimated along with the kinetic parameters appearing in the rate expressions. This is often done by introducing extra parameters, describing the initial state, and one way to eliminate them is by starting in a steady state. We report a generalisation of this approach to all systems starting on the centre manifold, close to a Hopf bifurcation. There exist biochemical systems where such data have already been collected, for example, of glycolysis in yeast. The initial value parameters are solved for in an optimisation sub-problem, for each step in the estimation of the other parameters. For systems starting in stationary oscillations, the sub-problem is solved in a straight-forward manner, without integration of the differential equations, and without the problem of local minima. This is possible because of a combination of a centre manifold and normal form reduction, which reveals the special structure of the Hopf bifurcation. The advantage of the method is demonstrated on the Brusselator.

Algorithms↗

Prodromal assessment with the structured interview for prodromal syndromes and the scale of prodromal symptoms: predictive validity, interrater reliability, and training to reliability.

As the number of studies related to the early identification of and intervention in the schizophrenia prodrome continues to grow, it becomes increasingly critical to develop methods to diagnose this new clinical entity with validity. Furthermore, given the low incidence of patients and the need for multisite collaboration, diagnostic and symptom severity reliability is also crucial. This article provides further data on these psychometric parameters for the prodromal assessment instruments developed by the Prevention through Risk Identification, Management, and Education (PRIME) prodromal research team at Yale University: the Structured Interview for Prodromal Syndromes and the Scale of Prodromal Symptoms. It also presents data suggesting that excellent interrater reliability can be established for diagnosis in a day-and-a-half-long training workshop.

Adolescent↗

The "Brisighella Terme" Project: effectiveness of preventive medicine in thermal ambient. Hematochemical parameters.

BACKGROUND: The main aims of the Brisighella Terme Project are: 1. evaluation of feasibility and effectiveness of preventive medicine intervention in thermal ambient; 2. identification of subjects with cardiovascular risk factors; 3. to give patients informations on risk factor correction. The data of this work concern the hematochemical parameters: CT, HDL-CT, LDL-CT, TG, ApoA1, ApoB, fibrinogen, GOT, GPT, CPK, glucose, uric acid. METHODS: CT, HDL-CT (after precipitation of the non-HDL fractions), TG, glucose, uric acid are evaluated by enzymatic-colorimetric reactions; GOT, GPT, CK by enzymatic method. ApoA1, ApoB, fibrinogen by immunoturbidimetric methods. LDL-CT is calculated by the Friedewald formula. RESULTS: 390 subjects spontaneously adhered to the Brisighella Terme Project. Of these subjects 38% requested laboratory service and we observed a 47% increment, from 1995 to 1996, with regard to this request. Females showed higher mean values than males of these parameters: CT, LDL-CT, HDL-CT, ApoA1, ApoB, fibrinogen, CPK. 55% of females had LDL-CT > 159 mg/dl, values considered high risk for cardiovascular diseases. 36% of males presented HDL-CT < 40 mg/dl, with 2 cases < 25 mg/dl. TG values > 399 mg/dl were found only in males (2%). We identified new cases of hypertriglyceridaemia, hypercholesterolaemia and hypoHD Laemia; these metabolic pathologies had not yet been diagnosed, whereas the patients already knew they were affected by hyperglicaemia or hyperuricaemia. CONCLUSIONS: People's interest in the Brisighella Terme Project, new case identification, the possibility of providing correct information about risk factors and healthy life style confirm the feasibility and effectiveness of preventive medicine in a thermal ambient.

Aged↗

Rectifying system-specific errors in NMR relaxation measurements.

15N spin relaxation parameters provide a powerful tool for probing the internal dynamics and thermodynamics of proteins. The biological insight provided by these experiments often involves interpretation of small changes in relaxation parameters. This, in turn, requires careful data analysis, especially in the identification and treatment of systematic error. While progress continues on reduction of experiment-specific errors associated with pulse sequences, system-specific sources of error have received far less attention. The impact of these errors varies between facilities, spectrometers, and biological samples. We demonstrate that performing a series of control experiments along with relaxation measurements can help identify, quantify, and isolate sources of system-specific error, and, in some cases, correct for systematic changes. We further demonstrate that control experiments can be performed without significant loss of spectrometer time, and lead to more accurate relaxation parameter values.

Nuclear Magnetic Resonance, Biomolecular↗

Edge detection in ultrasound images based on differential tissue attenuation rates.

Identification of the cardiac borders is a basic goal in the analysis of echocardiographic images. This is a requirement for various analysis purposes, such as the derivation of quantitative parameters, for wall motion analysis and for the creation of three-dimensional reconstructions. Border identification is usually accomplished by first identifying a set of generic edge points, then extracting the border of interest from the edge map. A variety of edge detection algorithms have been reported in the literature. However, the most commonly used methods are all based on the same underlying tissue discriminant, namely, the widely differing backscatter levels of the different types of tissue that form the cardiac borders, in particular, blood and myocardium. We have implemented a new method of edge detection based on an entirely different ultrasonic tissue characteristic--that of attenuation, the rate at which the tissue absorbs ultrasonic energy. This characteristic also differs widely between blood and myocardium, and therefore provides an alternative means of discriminating between these two types of tissue. The method searches for the abrupt change in attenuation rate that characteristically occurs at the blood/myocardium interface. At each point in the image, the average attenuation rates of the portions of tissue radially preceding and radially following the point are determined by least-squares regression. If one of these attenuation rates is sufficiently close to that of blood, and the other is sufficiently close to that of myocardium, then the point is flagged as an edge point. This paper describes how this method is implemented in software, and presents the results of applying the method to a library of sample images.

Algorithms↗

Sensitivity analysis for validating expert opinion as to ideal data set criteria for transport modeling.

Environmental fate modeling results are often used in risk assessment without adequately considering uncertainty in exposure predictions. Sensitivity analysis is fundamental to model validation and error prediction since sensitive model input parameters account for the largest variance in model prediction. Once identified, sensitive model input parameters can be used to propagate parametric uncertainty in numerical predictions. Output sensitivity to variation in input code sequences was investigated for the pesticide root zone model (PRZM 3) using Plackett-Burman analysis for six runoff and leaching data sets. The analysis utilized an incomplete block factorial design with even parameter weighting and uniform proportional input perturbation. Timing and duration of key period rainfall were assumed a priori to be dominant sensitive inputs. Thus, meteorological data were fixed, allowing identification of additional input components contributing to model sensitivity. Results validated expert modeler assumptions concerning parameters most critical for model validation. For leaching data sets, the application rate, soil bulk density (an indicator of available water-holding capacity), chemical partition coefficient, and pesticide degradation rates were commonly the most sensitive inputs. For runoff data sets, the in-crop runoff curve number was the most significant input governing pesticide loss in runoff and erosion flux. The chemical partition coefficient, soil and foliar decay rates, and soil bulk density were also common sensitive components for runoff predictions. These commonly observed sensitive components for runoff and leaching prediction need to be carefully considered in the design and conduct of relevant field studies, modeling assessment of such studies, and future improvements in algorithms for environmental transport modeling.

Meteorological Concepts↗

Identification of chemical substances by testing and screening of hypotheses. I. General.

The characteristic features and the constituents of an identification procedure for chemical substances are discussed. This procedure is a screening of identification hypotheses followed by experimental testing of each one. The testing operation consists of comparison of the values of the quantities measured with other measurement results or reference data, resulting in the Student's ratio, the significance level, the matching of spectra, etc. The performance and the correctness of identification are expressed as "identification uncertainty", i.e. the probability of incorrect identification. The statistical significance level and other similarity values in spectra, chromatography retention parameters, etc. are the particular measures of uncertainty. Searching of prior data and estimation of the prior probability of the presence of particular compounds in the sample (matrix) to be analysed simplifies the setting up and cancelling of hypotheses during screening. Usually, identification is made by the analyst taking into account measurement results, prior information and personal considerations. The estimation of uncertainty and rules for the incorporation of prior data, make the result of identification less subjective.

Journal Article↗

On-line identification and optimization of feed rate profiles for high productivity fed-batch culture of hybridoma cells using genetic algorithms.

In this paper, an on-line identification and optimization method based on genetic algorithms (GAs) has been used to optimize the productivity of a seventh-order nonlinear model of fed-batch culture of hybridoma cells. The parameters of the seventh-order nonlinear model are assumed to be unknown. The intention of this paper is to use GAs for (1) identifying the parameters of a seventh-order nonlinear model of fed-batch culture of hybridoma cells, and (2) determining the best feed rate control profiles for glucose and glutamine. The final level of monoclonal antibodies obtained by this method is then compared with the case where all the parameters are assumed to be known. It is found that the final level of monoclonal antibodies obtained by the on-line identification and optimization method is only about 3% less than the final level of monoclonal antibodies obtained by the case where all the parameters are assumed to be known. GAs proved to be a good alternative method for solving on-line identification and optimization problems.

Algorithms↗

Modeling of dose-response-time data: four examples of estimating the turnover parameters and generating kinetic functions from response profiles.

The most common approach to in vivo pharmacokinetic and pharmacodynamic modeling involves sequential analysis of the plasma concentration versus time and then response versus time data, such that the plasma kinetic model provides an independent function, driving the dynamics. However, response versus time data, even in the absence of measured drug concentrations, inherently contain useful information about the turnover characteristics of response (turnover rate, half-life of response), the drug's biophase kinetics (F, half-life) as well as the pharmacodynamic characteristics (potency, intrinsic activity). Previous analyses have assumed linear kinetics, linear dynamics, no time lag between kinetics and dynamics (single-valued response), and time constant parameters. However, this report demonstrates that the drug effect can be indirect (antinociception, cortisol/adrenocorticotropin (ACTH), body temperature), display nonlinear kinetics, display feedback mechanisms (nonstationarity, cortiso/ACTH) and exhibit hysteresis with the drug levels in the biophase (antinociception, body temperature). It is also demonstrated that crucial determinants of the success of modeling dose-response-time data are the dose selection, multiple dosing, and to some extent different input rates and routes. This report exemplifies the possibility of assigning kinetic forcing functions in pharmacodynamic modeling in both preclinical and clinical studies for the purpose of characterizing (discrimination between turnover and drug-specific parameters) response data and optimizing subsequent clinical protocols, and for identification of inter-individual differences.

Animals↗

Morphometric study on vascularization in the tail musculature of the anuran tadpole by scanning electron microscopy: I. Prometamorphic stage.

Vascularization in the tail musculature, which contains red and white muscle fibers, of the prometamorphic anuran tadpole was analyzed quantitatively by scanning electron microscopy (SEM). The sample was fixed in glutaraldehyde and osmium tetroxide; this was followed by freeze-fracturing in liquid nitrogen. As good ultrastructural preservation and reliable identification of capillaries were given by this technique, various morphometric parameters, cross-sectional capillary area in particular, could be measured exactly. In red muscle fiber that had a small cross-sectional area (2,060.1 micron2), high capillary density (1,283.5 capillaries/mm2) and a large cross-sectional capillary area (96.4 micron2) was found. Although white fiber (9,327.2 micron2) was 4.6 times greater than red fiber in cross-sectional fiber area, capillary density (95.8 capillaries/mm2) and cross-sectional capillary area (29.5 micron2) were 13.4 times and 3.3 times smaller than those of red fiber, respectively. From these morphometric values the following parameters were evaluated; (1) capillary/muscle fiber number ratio of red muscle fiber (2.64) was 3.0 times greater than that of white fiber (0.89); and (2) total cross-sectional capillary area per cross-sectional area of one muscle fiber was 44.0 times greater for red fiber (1,235.4 micron2)/10(4) micron2) than for white fiber (28.1 micron2)/10(4) micron2). Comparison of the latter parameter between the different fiber types may reflect the differences of real blood supply to them; i.e., red fiber was supplied a 44.0-times richer blood flow than white fiber. Advantages of morphometric study by SEM, and the relationship between obtained parameters for vascularization and blood supply to the different muscle fiber types, are discussed.

Animals↗

First-pass radionuclide angiocardiography in the determination of left-to-right cardiac shunt site in children.

To ascertain if the level of a left-to-right cardiac shunt can be reliably established by first-pass radionuclide angiocardiography with 99mTc-pertechnetate, 102 children have been studied; 19 without a shunt, 26 with an atrial septal defect (ASD), 45 with a ventricular septal defect (VSD), and 12 with a patent ductus arteriosus (PDA). Time-activity curves were generated over the right atrium and ventricle, and several quantitative parameters were derived from the curves. It was concluded that in the absence of right-sided valvular incompetence, the time-activity curves show the greatest abnormality in the cardiac chambers distal to the left-to-right shunt. The right ventricular curve is typically altered in ASD but cannot be relied upon to differentiate VSD or PDA. The identification of ASD is assisted by the use of two of the parameters, the count ratio C4:C1 and time ratio T2-T1.

Adolescent↗

Novel therapies for myelodysplastic syndromes.

BACKGROUND: The assessment of patients with myelodysplastic syndromes (MDS) and the choice of therapies remain challenging. New therapies are now emerging after the identification of molecular targets that result in improvement of hematologic parameters and may hold promise for the prevention of disease progression. METHODS: A review of the English literature was performed that included original articles and related reviews from MEDLINE (PubMed) and abstracts based on published meeting material. RESULTS: MDS is a heterogeneous group of disorders. Although current classification and prognostic schemes have proven valid to define subgroups, they are insufficient to take into consideration the significant biologic diversity of MDS. New molecular targets are identified as the mosaic of pathophysiologic pathways in MDS is being unraveled. Novel and targeted therapeutic agents, such as the inhibition of farnesyl transferases and receptor tyrosine kinases, more potent thalidomide analogs, and arsenic trioxide, have shown encouraging results and may offer durable benefit to patients with MDS. CONCLUSIONS: Although progress has been made in the understanding of clinical manifestations and some of the molecular pathways underlying ineffective hematopoiesis and leukemic transformation in MDS, intensive clinical and laboratory research continues to 1) identify further relevant pathophysiologic pathways, 2) better define MDS subgroups, and 3) develop new drugs based on a clearer understanding of disease biology.

Aged↗

Timely diagnosis of twin-to-twin transfusion syndrome in monochorionic twin pregnancies by biweekly sonography combined with patient instruction to report onset of symptoms.

OBJECTIVES: To assess the value of serial ultrasound examinations together with patient instructions to report the onset of symptoms in achieving timely detection of twin-to-twin transfusion syndrome (TTTS) in a cohort of monochorionic diamniotic twin pregnancies, and to evaluate sonographic TTTS predictors. METHODS: Timely detection of TTTS was defined as diagnosis before severe complications of TTTS occurred, such as preterm prelabor rupture of membranes, very preterm delivery (24-32 weeks of pregnancy), fetal hydrops, or intrauterine fetal death. During a 2-year period, a prospective series of 23 monochorionic twin pregnancies was monitored from the first trimester until delivery. At least every 2 weeks we performed ultrasound and Doppler measurements (nuchal translucency thickness, presence of membrane folding, estimated fetal weight, deepest vertical pocket, bladder filling, and Doppler waveforms of the umbilical artery, ductus venosus and umbilical vein). Measurements of TTTS cases were compared with those of non-TTTS cases matched for gestational age. Furthermore, patients were informed about the symptoms caused by TTTS, and instructed to consult us immediately in case of rapidly increasing abdominal size or premature contractions. RESULTS: In all four TTTS cases, the diagnosis was timely. At the time of diagnosis, one case was at Quintero Stage 1, two at Quintero Stage 2, and one at Quintero Stage 3. Two of the TTTS cases became apparent after the patients' feeling of rapidly increasing girth. The identification of TTTS predictors was successful with respect to one parameter: isolated polyhydramnios in one sac, without oligohydramnios in the other, preceded the ultimate diagnosis of TTTS in two of the four TTTS cases. All other ultrasound measurements of TTTS cases, prior to the diagnosis of TTTS, were within the range of measurements of non-TTTS cases. CONCLUSION: Biweekly ultrasound examinations, with special attention to the amniotic fluid compartments of both fetuses, combined with detailed patient instructions to report the onset of symptoms resulted in timely diagnosis of all TTTS cases and appears to be a safe program for monitoring monochorionic twin pregnancies.

Adult↗

A quantitative investigation of immunocytochemically stained blood vessels in normal, benign, premalignant and malignant human oral cheek epithelium.

The present study was designed to determine whether increased vascularity occurs during malignant transformation of human oral cheek epithelium. Nine normal (N) samples were taken from the resection margins of benign lesions; the pathological lesions were classified as chronic inflammation (CI; n = 11), fibrous hyperplasia (FH; n = 12), lichen planus (LIP; n = 8), dysplasia (DYS; n = 5), squamous cell carcinoma (SCC; n = 25; well differentiated [SCCWD]; n = 10; moderately to poorly differentiated [SCCMPD]; n = 15) and epithelium adjacent to carcinomas (EAC; n = 6). Sections were stained with monoclonal antibody (mAb) against vimentin using an ABC immunoperoxidase technique. All blood vessels present within a depth of 0.9 mm of lamina propria were quantified irrespective of their morphology. The blood vessel parameters quantified were volume density (VVBV, CT), number per unit area (NABV, CT), length per unit volume (LVBV, CT) and mean transverse sectional area (ABV). VVBV, CT increased significantly between normal and all pathological groups. Amongst the pathological groups, statistical differences were detected between CI and SCC, CI and EAC, FH and SCCWD, FH and EAC, LIP and SCC, LIP and EAC, DYS and SCCWD and DYS and EAC. The EAC group had the highest VVBV, CT and the values of NABV, CT and LVBV, CT were significantly higher in all the pathological groups when compared with the normal group. No significant differences were detected between any of the pathological group. The parameter ABV increased significantly between normal and DYS, FH, SCC, EAC, FH and EAC, FH and SCC, CI and EAC, CI and SCC, LIP and EAC and LIP and SCC. Spearman rank correlations detected a positive correlation between the severity of oral lesions and all of the blood vessel parameters. We conclude that a mAb against vimentin improved the identification of smaller blood vessels and the blood vessel data suggest that angiogenesis occurs in premalignant and malignant lesions of human oral cheek epithelium. Angiogenesis seems to play an essential role in sustaining the actively growing and transforming cells.

Adolescent↗

Compliance and satisfaction with foot compression devices: an orthopaedic perspective.

INTRODUCTION: Although mechanical methods of thromboembolic prophylaxis have proven efficacy, relatively little is known about levels of patient compliance in the usage of these devices. Based on anecdotal evidence, we hypothesised that levels of compliance are sometimes insufficient to provide adequate thromboprophylaxis in the clinical setting. We prospectively analysed the use of foot pumps in 30 orthopaedic patients undergoing lower limb arthroplasty in order to assess levels of both patient compliance and satisfaction. MATERIALS AND METHODS: The study was carried out over a 5-month period. Levels of compliance were measured by using a system of random "spot checks". Each patient completed a questionnaire before discharge. Parameters investigated included comfort levels while using the device and identification of factors about the device which the patients disliked. RESULTS: Three hundred and ninety-one measurements were made. The utilisation of the device was found to be effective in 40.2% of measurements. We detected an overall progressive decline in the level of compliance as post-operative time increased [P < 0.001, Chi-square (chi(2)) test]. The average level of patient "comfort" was 7.1 on a visual analogue scale of 0-10. Sleep disturbance was reported by 57% of patients, while 43% complained of "heat intolerance". We have also shown that compliance is significantly reduced in those who complained of "sleep disturbance" while using the foot pumps (t-test, P < 0.05). CONCLUSIONS: These findings suggest that satisfactory levels of compliance can be difficult to achieve in the clinical setting despite the proven efficacy in clinical trials of this device. Greater emphasis on patient education may help improve compliance. Unless compliance can be strictly enforced, it appears advisable to only use foot pumps in combination with other forms of pharmacological prophylaxis.

Adult↗

Evaluation of isoelectric focusing running conditions during two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis: variation of gel patterns with changing conditions and optimized isoelectric focusing conditions.

Five major isoelectric focusing (IEF) parameters--volt-hours; concentrations of acrylamide, NaOH, and H3PO4; and equilibration time--were systematically varied to determine the effect of each on two-dimensional IEF/sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel patterns and to optimize IEF conditions. Alterations in each parameter affected the gel pattern, frequently causing uncertainty in the identification of spots between conditions. The results emphasize the need for internal analytical consistency, and indicate that gel pattern comparisons between laboratories can be complicated if different IEF conditions are employed. The systematic evaluation indicated that optimized patterns were obtained when increased concentrations of NaOH and H3PO4 (to 50 and 25 mM, respectively) and run durations of 10,000 V-h or longer were used.

Buffers↗