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PubMed1984

Comparison of tests for the diagnosis of spontaneous encephalitozoonosis in rabbits.

The effectiveness of immunofluorescence, complement fixation, microagglutination serologic tests, intradermal skin test, and detection of histologic lesions were compared for use in diagnosis of spontaneous encephalitozoonosis in rabbits. The India ink and microbead agglutination reactions were compared with immunofluorescence and complement fixation by testing 11 single or pooled sera. Serologic tests correlated best with each other and less well with intradermal tests or presence of lesions. Immunofluorescence, India ink reaction and microbead agglutination were equally useful in detecting antibodies to Encephalitozoon cuniculi. The intradermal test correlated best with the presence of detectable lesions.

Agglutination Tests↗

PubMedDate not supplied

Effects of myocardial infarction on catheter defibrillation threshold.

Because the automatic implantable defibrillator may be used in patients having ischemic heart disease, it is important to know whether myocardial ischemia changes the threshold for ventricular defibrillation under experimental conditions simulating automatic internal defibrillation. We determined changes in ventricular defibrillation threshold following coronary occlusion, using an electrode catheter designed for use with an automatic implantable defibrillator. Acute myocardial ischemia was produced without thoracotomy in 10 dogs (experimental group) by embolization with a plastic bead injected via a catheter into the left coronary artery. A control group of 4 dogs had only saline injected into the artery. Defibrillation threshold was measured at 15-min intervals from 1 hour before embolization to 2 hours after embolization. In the control group, voltage, current, energy, and impedance were unchanged after injection of saline into the coronary artery, and india ink perfusion revealed no ischemic areas. In the experimental group postembolization threshold current and energy were significantly higher than preembolization values: 0.47 vs 0.40 A/kg and 1.01 vs 0.80 J/kg, respectively (p less than 0.01). The magnitude of the peak change in threshold current after embolization was positively correlated (r = 0.79) with the size of the ischemic zone, determined by weighing unstained areas after india ink perfusion. Defibrillation threshold for a catheter electrode configuration increases for at least 2 hours following onset of acute myocardial ischemia. This finding must be accounted for in the design and use of an automatic implantable defibrillator.

Animals↗

PubMed1981

Microangiographic representation of the microvascular system in bone tissue: a vital microscopic evaluation in the rabbit.

A titanium chamber, enabling in situ studies of living bone, was inserted into the adult rabbit metaphysis. The true vascular state of an observable bone tissue compartment was microscopically defined after autologous grafting of the chamber and a surrounding bone collar. Micropaque and India ink microangiography, respectively, were performed under direct, simultaneous inspection in a vital microscope. In 20 animals Micropaque and India ink, on the average, filled out less than 50% of the actually functioning vessels. Resting vessels were not responsible for the poor vascular delineation. Either a direct capillary blockage or an indirect blockage due to intravascular mixing of contrast media and blood occurred.

Animals↗

PubMed1982

[In vitro tumor sensitivity tests to chemotherapeutic agents by the suppression of dehydrogenase activity].

A test system using dehydrogenase activity for predicting the response to chemotherapeutic agents against cancer cells was introduced. Agar plate assay, INK which test, and SDI test commonly employed in the clinical study were also reviewed. Agar plate assay resembles antibiotic disc sensitivity test. The cancer uniformly suspended in the agar medium was exposed to drugs on paper discs for few hours. After removal of the disc, methylene blue or 2, 6-dichlorophenol indophenol was applied as a dehydrogenase indicator. INK test was introduced by Nishioka et al. in 1957. Several fragments of fresh cancer tissue were incubated with chemotherapeutic agents in roller test tubes. Twenty-four hours later, 2, 6-dichlorophenol indophenol was applied as a dehydrogenase indicator. To develop a simple, rapid, and comparable test, SDI test was introduced by us in 1964. A fresh cancer tissue was minced and made into the cell suspension. After cancer cells were exposed to chemotherapeutic agents, the activities of succinic dehydrogenase of the treated cells were measured by the reduction of 2, 3, 5-triphenyl tetrazolium chloride. Some points to be improved were investigated and discussed.

Antineoplastic Agents↗

PubMed1980

Early ultrastructural changes in papain-induced experimental emphysema.

These studies show that very soon after exposure of canine lungs to crude papain mixed with a marker (India ink), the alveolar surface-active lining was both morphologically and functionally altered, and alveolar macrophages were destroyed in significant numbers. These changes occurred before other identifiable major alterations. The morphologic changes were characterized by replacement of the normal alveolar surface lining by an amorphous material adhering to the alveolar surfaces and penetrating the pores of Kohn, which were significantly enlarged. Type II epithelial cells and alveolar macrophages were increased in number, with a significant proportion of the latter observed to be disintegrating. Polymorphonuclear leukocytes were also noted in increased numbers, but this was also observed in control lungs (instilled only with saline plus India ink). Functionally the alveolar surface lining as studied in lung extracts showed an abnormal stability index after either in vivo or in vitro exposure to crude papain. These findings suggest that the early effects of this exogenous protease on the lung are interrelated, alveolar surface lining injury appearing to set the stage for accelerated macrophage lysis, with the probable release of phagocytosed exogenous protease as well as intracellular endogenous proteases. These events may represent early steps in the pathogenesis of experimental papain-induced pulmonary emphysema.

Animals↗

PubMed1978

Allergic contact dermatitis to 1,4-bis(isopropylamino)anthraquinone. Caused by a felt-tip marker pen.

Adult allergic contact dermatitis to the black ink of a felt-tip marker pen occurred in two adult subjects. In both subjects the cutaneous reaction did not require photoactivation. Patch testing with the ingredients in the black ink demonstrated cutaneous sensitivity to the black dye. Further patch testing with the components of the black dye revealed cutaneous sensitivity in both subjects to the blue component, 1,4-bis(isopropylamino)anthraquinone, commonly known as Colour Index Solvent Blue 36.

Adult↗

PubMed1978

[Method of intravital injection of the internal blood vessels of organs for experimental-morphological research].

Ten minutes before the injection the rat is given heparin (500 units per 1 kg weight) intradermally. To the narcotized animal, the needle with the 1 ml syringe is inserted and 3--5 ml of blood are aspirated (about 2% of the body mass). Then with the same needle another syringe of 10 ml volume containing filtrated and heated up to 40 degrees C undiluted Indian ink is injected. The amount of the Indian ink injected is 8--10 ml per 100 g of the body mass. We can consider the injection oa success if the mucose of the tongue, the skin of the concha auriculae, the sclera of the eye are promptly stained during the injection and the tail vessels are well filled.

Animals↗

PubMed1994

Laboratory evaluations of toothbrush interproximal penetration ratios.

Access to interproximal tooth surfaces by toothbrush bristles has been associated with plaque removal. The ability of toothbrushes to assess the interproximal area was assessed with a new laboratory apparatus by measuring the extent of bristle-to-tooth contact in simulated toothbrushing with ink-coated toothbrush bristles. Toothbrush types evaluated were the bi-level trim Advanced Design Reach Soft, the ripple trim Crest Complete Large Soft, the flat trim Colgate Plus Full Head Adult Soft, and the flat trim Oral-B 35. Six replicates of each toothbrush were tested. Denture teeth in a Columbia Dentoform, mounted at 45 degrees, were brushed at a 300 g brushing weight for 15 horizontal strokes. Tooth #15 (upper left second molar) was then removed for measurement and the interproximal distance of ink deposition was determined under 10x magnification from the plane of the buccal contour, along the mesial side of the tooth in the lingual direction at the approximate vertical midpoint of the proximal surface. This was compared in ratio to the same measurement from the buccal contour to the midline. Mean penetration ratios were 0.84 for the Advanced Design Reach, 0.79 for Crest Complete, and 0.78 for both Colgate Plus and Oral-B 35 (overall standard error = 0.01). A one-way ANOVA showed a highly significant difference (p < 0.001) demonstrating that Advanced Design Reach penetrated significantly farther than the toothbrushes with flat or ripple trim bristle patterns. The data suggest the bi-level trim in the Advanced Design Reach toothbrush offers better penetration into the area between teeth, and may provide superior clinical or plaque removal compared to the other toothbrushes tested.

Analysis of Variance↗

PubMed1993

[Myocardial protection: is retrograde equal to antegrade perfusion?].

The adequacy of retrograde delivery of cardioplegic solution to the right ventricle ist controversial. To evaluate this issue, we excised the plegic heart in 11 bovine experiments and infused an India ink solution (10 ml of India ink in 300 ml NaCl 0.9%) into the coronary sinus (n = 7) at a pressure of 60 cm H2O and into the aortic root (n = 4) at a pressure of 120 cm H2O. After fixation, the ventricles were cut in 11 transversal slices. The portion of coloured (= perfused) ventricular myocardium was calculated with computer-aided morphometric analysis. With antegrade infusion, 95 +/- 5% (mean +/- standard deviation) of the left ventricular volume (left ventricular free wall plus interventricular septum) was stained, with retrograde infusion 94 +/- 3%. Perfusion of the right ventricle was significantly lower with retrograde infusion (antegrade infusion 93 +/- 8%, retrograde 45 +/- 13%, p < 0.001), especially in the basal segments (basal vs. apical: 16 +/- 26% vs. 82 +/- 5%, p < 0.001). The adequate delivery of retrograde infusion to the left ventricle and septum allows good left ventricular myocardial protection with retrograde cardioplegia. Because the retrograde delivery to the right ventricle is markedly inadequate and nonuniform, the quality of right ventricular protection with retrograde cardioplegia has to be questioned.

Animals↗

PubMed1993

Marking margins of tumorectomies and excisional biopsies to facilitate histological assessment of excision completeness.

Assessment of tumor margins for completeness of excision is important for local control of neoplastic masses, particularly those that are potentially curable with surgery. Of the margin-marking techniques available, India inking seems to be the most practical way for veterinary clinicians to help pathologists accurately distinguish between surgical margins and margins created during sample processing. India inking prevents misinterpretation of contaminated processing margins as inadequate surgical excisions; however, a strongly definitive decision that an excision is complete cannot be made with any margin-marking technique because it is impossible to examine the entire surface of every margin.

Animals↗

PubMed1993

Parasitological and clinical studies on human scabies in Cairo.

This study is a parasitological and clinical study on human scabies. This study was carried out on 100 patients attending the Dermatology Outpatient Clinic at Ain Shams University Hospitals, including 54 males and 46 females. All patients were subjected to detailed history taking, complete dermatological examination including Skin Scraping Test and Burrow Ink Test. Younger patients attended the dermatology clinic earlier than older patients, who usually delayed their visits until complications occurred. Scratching, erythematous papules and secondary infected lesions were the commonest lesions. The hands, wrist and external genitalia were the most frequently affected sites while the feet, ankles, knees and back were the least affected. Burrows could only be detected in 40% of patients. The most frequent sites were the web spaces, external genitalia, and finger sides. Burrow Ink Test was positive among 85% of patients with burrows while mites could be identified by Skin Scraping Test in 55% of patients. The most frequent clinical manifestations in the parasitologically positive patients were itching, burrows, and papules, and the most frequent sites were the web spaces, and the finger sides. Multiple sites affection was the characteristic feature among the preschool age children who represented 14% of cases.

Age Factors↗

PubMed1977

A comparative study of the microcirculation in the guinea-pig thymus, lymph nodes and Peyer's patches.

The circulatory patterns in three lymphoid tissues were compared macro- and microscopically. Indian ink or a viscous silicone rubber compound were used as contrast materials and serial 75 micronm sections examined. The findings were checked with the benzidine stain for red blood corpuscles in uninjected tissues. Thymic lobules showed a "through circulation"--capillaries penetrating the cortex from within outwards and draining into surface veins, comparable with the circulation in liver lobules. By contrast, lymph nodes and Peyer's patches showed a looped capillary distribution. The outer thymic cortex and primary follicles in lymph nodes contained the maximum capillary density, correlating with the highest mitotic index of thymocytes and lymphocytes in the respective tissues. The lowest vascular density in the thymus was in the medulla, particularly in Hassall's corpuscles, in secondary follicles of lympho nodes and Peyer's patches, which correlates with the storage capacity of antigens and antibodies at those sites. Postcapillary venules in lymph nodes and Peyer's patches showed a characteristic pattern with Indian ink. These venules were absent from the thymus.

Animals↗

PubMed1995

A technique for identification of cellular proteins by western immunoblotting and immunoautoradiography of 2-D PAGE.

A major effort of our work has been devoted to the identification, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), of lymphoid polypeptides that are involved in lymphoid proliferation or differentiation. We have encountered problems during this effort pertaining to the exact localization of a polypeptide(s) in the silver stained gels that is recognized by western immunoblotting or proteins detected by autoradiography. In this paper, we present a method, using India ink stained/immuno-staining replica of 2-D gel nitrocellulose membrane (NCM) or India ink stained coupled with autoradiography in the case of phosphoproteins, which allows us to exactly localize the polypeptide spots detected by these methods in the silver stained 2-D PAGE. This method is expected to popularize and widen the use of 2-D PAGE technology in the investigation of cellular polypeptides.

Autoradiography↗

PubMedDate not supplied

Histologic features of chorioamnion membrane rupture: development of methodology.

This study developed a set of histologic features that will allow subclassification of placentas with preterm premature rupture of membranes. Placentas were obtained from patients participating in a multi-institutional NICHD Maternal-Fetal Medicine Unit Network study of antimicrobial therapy after preterm premature rupture of membranes. The rupture site was sampled by inking the open sac margin and rolling a membrane strip in four quadrants from the ink to the placental margin. Independently, four pathologists used a provisional feature list to score the slides from 15 placentas. A concordance analysis was performed on those results. With those results, the slides were reviewed concurrently to discover the source of disagreements and to revise the feature list. The sampling method frequently demonstrated a rupture site with histology distinct from that of the remainder of the membranes. After review of the preliminary scoring results, 29 features of membrane histology present in preterm premature rupture could be objectively described with agreement among four pathologists. The feature list allows both novel and commonly recognized histologic features of fetal membranes to be recorded with objectivity. This list, with the described sampling technique, is presented as a tool for clinical correlation in studies of membrane rupture, especially in preterm, premature rupture.

Amnion↗

PubMed1997

Reversibility in blood-brain barrier, microcirculation, and histology in rat brain after decompression.

To examine the changes in blood-brain barrier (BBB), cerebral microcirculation, and histology from 15 min to 72 h after decompression, 90 rats were exposed to experimental compression to 6 atm abs air for 90 min and subsequent rapid decompression. The disruption of BBB was examined by Evans blue extravasation. The cerebral microcirculation was demonstrated by perfusion with India ink. The area stained with Evans blue and the regions of defective filling with India ink, observed immediately after decompression decreased in size with time and were undetectable 3-24 h after decompression. The edematous brain tissue with enlarged perivascular space and darkly stained nerve cells also decreased to the uncompressed control level 1-24 h after decompression. These reversible dysbaric changes, however, reappeared 48-72 h after decompression. The different mechanisms, the physicochemical effects of microbubbles, and the maturation phenomenon after temporary brain ischemia induced by dysbaric microbubbles may be involved in the brain damage after decompression sickness.

Animals↗

PubMed1997

The gracilis myofasciocutaneous flap: vascular anatomy and clinical application.

Unreliable skin perfusion has been reported frequently in the gracilis myocutaneous flap, resulting in moderately high partial flap necrosis. We modified the traditional myocutaneous operative technique by including all available regional fascia and created a myofasciocutaneous flap with increased skin viability. In addition, we defined the arterial anatomy of the flap that contributes to enhanced flap survival. In a cadaver study, blue latex was injected into the external iliac arteries of 11 cadaveric legs and the gracilis myofasciocutaneous flap dissected. Selective ink injection of the pedicle and perforating vessels also was performed in 8 legs. Two additional legs were injected with a barium-latex mixture, cut into 2-cm-thick transverse sections, and radiographed. Dissections demonstrated arterial connections between the pedicle vessel (medial femoral circumflex artery) and fasciocutaneous perforating vessels from the superficial femoral artery. Perforating vessels contribute to an axially oriented fascial network that supplies the overlying skin. Selective ink injections demonstrated the individual primary cutaneous vascular territories for each perforator. Radiographs of 2-cm-thick transverse sections confirmed the presence of arterial connections between the pedicle and the superficial femoral artery perforators. Twelve patients, previously pelvically irradiated, then underwent harvest of 18 large, longitudinally oriented (8-cm-wide, up to 30-cm-long) gracilis myofasciocutaneous flaps. All fascia beneath the skin paddle was taken in continuity with the deep fascia surrounding the gracilis muscle to minimize disturbance of any connecting vasculature held within the fascia. Patients were followed for an average of 12.1 months (range 3 to 31 months). Minor complications related to the flaps occurred in 6 of 12 patients (50 percent), i.e., seromas, mild wound infections, and a partial dehiscence; however, vascularity was excellent with no partial or complete flap necrosis. All wounds healed completely.

Adult↗

PubMed1997

Quantification of oxygen-induced retinopathy in the mouse.

PURPOSE: To describe a quantifiable model of vascular proliferation in oxygen-induced retinopathy (OIR) in the mouse. METHODS: Neonate Quackenbush mice were subjected to high-ambient oxygen (approximately 95%) for the first 5 days after birth, effecting a total inhibition of retinal vascular growth. Animals then were returned to room air, and the rates of subsequent vascular development in the plane of the retina and estimates of retinal capillary density were measured from flatmounts of ink-perfused eyes. Observations were confirmed with fluorescein isothiocyanate-lectin labeling of the peripheral vasculature. Abnormal growth of vascular sprouts into the vitreous was recorded from cross-sections. Observations in OIR were compared against those of age-matched control animals. RESULTS: The slower rate of retinal revascularization in OIR mice was quantified and compared against the normal rate. Lectin-binding studies confirmed the reliability of ink preparations. The number of vitreous sprouts in OIR peaked 8 to 10 days after animals were returned to room air (13 to 15 postnatal days). Sprouts then regressed, to disappear by postnatal day 20. In all respects, bar a slightly lower peripheral capillary density, the normal retinal vascular pattern was achieved in OIR within 15 days of exposure to room air (as opposed to the 10 days required in control mice). CONCLUSIONS: The protocol described for quantifying retinal proliferation in mouse OIR is reproduced readily, and the data recorded here will allow the effectiveness of subsequent treatments that may affect retinal vascular growth to be evaluated better.

Anatomy, Cross-Sectional↗

PubMed1997

Lack of spontaneous regression of tubular adenomas in two years of follow-up.

OBJECTIVE: Change in colon polyp size over time has not been well characterized. It has been inferred that some polyps will increase in size, leading to an increased risk of progressing to cancer, whereas other polyps may spontaneously regress. To develop a better understanding of the natural history of colon polyps, we prospectively investigated change in polyp size over a 2-yr period. METHODS: Patients were enrolled if they had an endoscopically detected proximal rectal or sigmoid polyp measuring 3-9 mm. The index polyp site was then permanently marked with an adjacent India ink tattoo to allow definitive future localization of the polyp. Patients underwent flexible sigmoidoscopy at 6-month intervals, and at each examination, the polyp size was carefully measured with open biopsy forceps. After a maximum of 2 yr, each polyp was removed and the histology determined. RESULTS: Thirty polyps were followed in 26 patients who completed the study. Twelve polyps were tubular adenomas (TA), one was tubulovillous, 14 were hyperplastic polyps (HP), two had no pathological diagnosis, and one was a leiomyoma. HP were more likely to decrease in size than were TA. Three polyps demonstrated fast growth rates (2-4 mm/yr), and all were TA. Two polyps were removed early because their size had reached 1 cm or more. Both of those polyps were TA. No polyps regressed completely during the 2 yr of the study; neither did we find consistent linear growth rates. CONCLUSIONS: In contrast to prior reports, in this study, after polyps had been definitively localized with India ink, we observed no complete polyp regressions.

Adenoma↗