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PubMed1966

The MEDLARS project at the UCLA Biomedical Library.

The National Library of Medicine has established at the Biomedical Library, UCLA, a remote MEDLARS search station. The agreement between the libraries provided that the Biomedical Library in cooperation with the UCLA Health Sciences Computing Facility would reprogram MEDLARS for the IBM 7094 computer, provide personnel to be trained in machine searching at NLM, and engage in a pilot demonstration of search service to the medical staff of UCLA. This paper describes the place of the search section in the organization of the Biomedical Library and the training of personnel at NLM (the article by Garvis in this issue of the Bulletin relates the problems encountered in making two different computer systems compatible). Search service had not begun when this paper was written; it was scheduled to begin late in 1965. Plans for the future include the enlargement of the UCLA MEDLARS staff and extension of search service to a larger geographical area.

California↗

PubMed1966

Centralization vs. decentralization in medical school libraries.

Does the medical school library in the United States operate more commonly under the university library or the medical school administration? University-connected medical school libraries were asked to indicate (a) the source of their budgets, whether from the central library or the medical school, and (b) the responsibility for their acquisitions and cataloging. Returns received from sixtyeight of the seventy eligible institutions showed decentralization to be much the most common: 71 percent of the libraries are funded by their medical schools; 79 percent are responsible for their own acquisitions and processing. The factor most often associated with centralization of both budget and operation is public ownership. Decentralization is associated with service to one or two rather than three or more professional schools. Location of the medical school in a different city from the university is highly favorable to autonomy. Other factors associated with these trends are discussed.

Libraries, Medical↗

PubMed1967

The program of the Francis A. Countway Library of Medicine.

The planning of the Countway Library took place in an atmosphere of community interest and support. Architectural planning was effective and harmonious. The building functions well. The program of the Library is briefly described. Serials records, involving 17,000 titles, have been mechanized. A rare books program has been initiated. Since building occupancy, the Library has substituted copy service for the lending of periodicals. For an experimental period, the copy service was cost free. The Aesculapian Room, for non-medical recreational reading, has had the generous support of the Aesculapian Club. Plans for regional library service in New England are based upon an historic commitment of the Boston Medical Library. A MEDLARS regional search center and a national Vision Information Center have been established in the Countway.

Information Systems↗

PubMed1967

Health science libraries of national, state, and local medical organizations.

This second survey of medical society-sponsored libraries has been expanded to include national association libraries in allied medical fields, as well as special libraries which do not fall into categories established for the MLA survey of health science libraries. A total of fifty-eight libraries in this subset have been identified, and selected characteristics have been measured. Observations are made concerning methodology, user population, and services.

Library Surveys↗

PubMed1980

JCAH accreditation and the hospital library: a guide for librarians.

The continuing effort to develop standards for libraries in health care institutions has resulted in the creation of two broad groups of standards: (1) quantitative and specific, and (2) qualitative and flexible. The library standards of the Joint Commission on Accreditation of Hospitals (JCAH), a major example of the second type, were revised and expanded considerably in 1978, bringing them into line with standards for other hospital departments. Possible areas of unclarity or difficulty for the librarian in complying with the revised JCAH standards are discussed, including those relating to staffing, consultants, library technicians, analysis of resources, assessment of needs, documentation, policies and procedures manuals, and the library committee. The JCAH site visit, including preparation of the Hospital Survey Profile, gathering information for the surveyor, and the summary conference, offers opportunities to librarians to participate in an institution-wide effort, to upgrade management practices, and to demonstrate the need for, and effectiveness of, library services in their hospitals.

Accreditation↗

PubMed1995

Identification of novel peptide antagonists for von Willebrand factor binding to the platelet glycoprotein Ib receptor from a phage epitope library.

We have constructed a fusion phage epitope library in the filamentous bacteriophage fuse5. The library was made by inserting a degenerate oligonucleotide which encodes 15 variable amino acids into the NH2-terminal region of the phage gene III protein. This library, containing over 10(7) different epitope bearing phage, has been used in an attempt to identify inhibitors of the von Willebrand factor (vWF)-platelet Glycoprotein Ib interaction. The library was screened with a monoclonal antibody (RG46) that recognizes the GPIb binding domain of vWF (amino acids 445-733). A total of 30 clones falling into 8 classes have been identified that react with the RG46 antibody. Isolates from all 8 classes are positive by immunoblot analysis. The amino acid sequence of the gene III fusion protein from positive clones showed a strong homology to the known RG46 epitope. Peptides identified from the screen were synthesized and used to demonstrate that some of the synthetic peptides exhibited inhibitory activity towards ristocetin induced binding of vWF to the GPIb receptor. Thus, we have demonstrated that screening a fusion phage epitope library with a monoclonal antibody that inhibits vWF binding to the GPIb receptor can be a useful tool not only for mapping antibody recognizing determinants, but also can serve as a source for identifying novel peptides that are antagonists for vWF binding to the platelet GPIb receptor.

Amino Acid Sequence↗

PubMed1994

Identification of a Src SH3 domain binding motif by screening a random phage display library.

A phage display library was constructed in the filamentous bacteriophage fuse5. The library was made by inserting a degenerate oligonucleotide which encodes 15 variable amino acids into the NH2-terminal region of the phage gene III protein. This library, containing over 10(7) different phage, was screened with a glutathione S-transferase (GST) fusion protein containing the Src homology 3 (Src SH3) domain and a protein kinase A phosphorylation site (GST/PKA/Src SH3). A family of proline-rich sequences was isolated following four cycles of enrichment and amplification. Phage containing these sequences were shown to specifically bind to the GST/PKA/Src SH3 protein but not to GST/PKA only. A comparison of the inferred amino acid sequence of the different phage clones revealed a consensus sequence, RPLPXXP, which conforms to a Src SH3 domain binding motif identified independently during an affinity screen of a lambda-lox mouse embryo cDNA library using a 32P-labeled Src SH3 protein fragment as the probe (Y. Ivashchenko, manuscript in preparation). Peptides based upon the 7-amino acid SH3 binding domain core motif displayed strong binding to both the Src and to the Fyn SH3 domains, but failed to bind to the SH3 domain of p21 Ras-GTPase-activating protein (Ras-GAP) and other proteins. We anticipate that further screening of the phage display library will be a useful tool for the rapid identification of additional SH3 domain binding sequences and will also help to establish the essential core motifs that define the specificity of interactions among the diverse proteins containing SH3 domains and those containing SH3 binding motifs.

Amino Acid Sequence↗

PubMed1994

Isolation of neutralizing anti-C5a monoclonal antibodies from a filamentous phage monovalent Fab display library.

A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection vs rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5 the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning four Fabs reactive with rC5a were isolated. Two bound preferentially with and neutralized C5a. Competitive biopanning of phage display libraries may increase the probability of identification of Abs of the desired specificity.

Amino Acid Sequence↗

PubMed1993

Characteristics of services and educational programs in libraries serving problem-based curricula: a group self-study.

A group of librarians from institutions with formal problem-based learning (PBL) curricula tracks began meeting informally in 1989 to explore the common elements among libraries serving PBL curricula. In 1991, a self-study was undertaken to delineate library services in these schools and also to identify the mechanisms that enable libraries to respond directly to the PBL curriculum. This paper reports results of this self-study, including findings regarding services, collection access, library access, and, particularly, areas that facilitate the PBL process, such as availability of on-demand and tailored user education, end-user literature searching, and electronic mail. Formal and informal library instruction, both optional and required, is described. The self-study also identified structured and unstructured methods of student access to PBL curriculum resources, including bibliographies, formally defined reserve collections, and student and faculty resources. The many roles of the professional librarian within the PBL curriculum are described; librarians may serve as traditional service providers, as resource persons, as faculty, or as tutors or facilitators in PBL curriculum sessions.

Curriculum↗

PubMed1993

A problem-based learning curriculum in transition: the emerging role of the library.

This case study describes library education programs that serve the University of New Mexico School of Medicine, known for its innovative problem-based learning (PBL) curricular track. The paper outlines the specific library instruction techniques that are integrated into the curriculum. The adaptation of library instruction to a PBL mode of medical education, including the use of case studies, is discussed in detail. Also addressed are the planning processes for the new PBL curriculum scheduled for implementation in 1993, including the activities of library faculty and staff and the probable new role of the library in the new curriculum.

Computer-Assisted Instruction↗

PubMed1993

How hyper are we? A look at hypermedia management in academic health sciences libraries.

Advances in instruction-delivery technology have a direct impact on academic media centers. New technology challenges librarians philosophically, financially, and ethically to provide access to information and instructional systems. Each institution has a unique set of circumstances governing decisions to provide access to hypermedia. If patron needs are met satisfactorily through labs outside the library, it may not be necessary for the library to incorporate hypermedia into its collection. Other library media centers may serve as a main point of access, or a substantial alternative computing resource may exist in departments or professional schools. Regardless of which route is taken, hypermedia is a viable instructional delivery system and can coexist with traditional services. Future studies on various aspects of hypermedia and multimedia management should be encouraged. Academic health sciences librarians would benefit from the study of hypermedia and multimedia collection-development policies, equipment, and personnel management. As computer networking of multimedia and image databases becomes available, it will be interesting to see the role academic health sciences libraries assume in integrating these data-bases with traditional information-delivery systems. Changing technology and instructional methods will affect budgets as well as library relationships with academic departments and computing centers.

Computer Communication Networks↗

PubMed1993

Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR.

We describe here the production of complex libraries enriched in sequences from each human chromosome type, starting with only a few thousand sorter-purified chromosomes. In this procedure, DNA is extracted from the sorted chromosomes, digested to completion by using the frequently cutting restriction endonuclease Sau3A1, and ligated, on each end, to an adaptor oligonucleotide. These fragments are then amplified using PCR with a sequence homologous to the adaptor oligonucleotide as a primer. We have used this procedure to produce PCR libraries for each of the 24 human chromosomes. These libraries were characterized by gel electrophoresis and found to be composed of a continuum of sequences ranging in size from a few hundred to approximately 1,000 bp. The libraries, when used as probes for fluorescence in situ hybridization, stained the target chromosomes more or less continuously, even after PCR amplification for more than 200 cycles. These libraries are useful as hybridization probes to facilitate molecular cytogenetic studies and as sources of probes either for identification of polymorphic short tandemly repeated sequences or for development of sequence-tagged sites.

Base Sequence↗

PubMed1996

[Cosmid libraries containing DNA from human chromosome 13].

We characterized two cosmid libraries constructed from flow-sorted chromosome 13 at the Imperial Cancer Research Fund (ICRF), UK (13,000 clones) and Los Alamos National Laboratory (LANL), USA (17,000 clones). After storage for two years, clones showed high viability (95%) and structural stability. EcoR I and Hind III restriction patterns were studied in more than 500 ICRF and 200 LANL cosmids. The average size of inserts was shown to be 35-37 kb in both the libraries. Most cosmids (83% and 93% of ICRF and LANL libraries, respectively) exceed the lower size limit of DNA fragments that can be packaged and represent a good source for physical mapping of chromosome 13. Total length of inserts is four and five genome equivalents in the ICRF and LANL libraries, respectively. ICRF cosmids showed hybridization to 22 of 24 unique probes tested, which corresponds to a 90% probability of having any DNA fragment represented in the library. More than 1 Mb of chromosome 13 is overlapped by 90 cosmids of 22 groups revealed. A chromosomal region of more than 150 kb, containing the ATP1AL1 gene for alpha-1 peptide of Na+, K(+)-ATPase, is covered by 12 cosmids forming a contig. The results of restriction and hybridization analyses are stored in a CLONE database. These data and all the cosmids described are publicly available.

Chromosome Mapping↗

PubMed1996

Detection of potential cytokine gene(s) in an Alu-PCR library from the human chromosome 4.

Jumping and linking libraries have been constructed in order to have more information of chromosome 4. Two types of jumping libraries (Not I and Xma III jumping) and one linking library were prepared. The jumping clones represent DNA regions between two rare-cutting enzymes sites (such is Not I or Xma III) whereas linking clones represent only the area which surrounds the rare-cutting site. These clones were used as target DNA in Alu-PCR. The Alu-PCR fragments were cloned into pGEM-T vector and transformed into Escherichia coli JM 109 competent cells. The colonies were screened for inserts by restriction analysis. More than 50% of the colonies were shown to contain inserts. Inserts from each library were screened by PCR. One of the jumping libraries appeared to have inserts containing CpG islands and AT-rich sequences.

Chromosomes, Human, Pair 4↗

PubMed1998

Definition of natural T cell antigens with mimicry epitopes obtained from dedicated synthetic peptide libraries.

Progress has recently been made in the use of synthetic peptide libraries for the identification of T cell-stimulating ligands. T cell epitopes identified from synthetic libraries are mimics of natural epitopes. Here we show how the mimicry epitopes obtained from synthetic peptide libraries enable unambiguous identification of natural T cell Ags. Synthetic peptide libraries were screened with Mycobacterium tuberculosis-reactive and -autoreactive T cell clones. In two cases, database homology searches with mimicry epitopes isolated from a dedicated synthetic peptide library allowed immediate identification of the natural antigenic protein. In two other cases, an amino acid pattern that reflected the epitope requirements of the T cell was determined by substitution and omission mixture analysis. Subsequently, the natural Ag was identified from databases using this refined pattern. This approach opens new perspectives for rapid and reliable Ag definition, representing a feasible alternative to the biochemical and genetic approaches described thus far.

Antigens, Bacterial↗

PubMed1998

Citation analysis in journal rankings: medical informatics in the library and information science literature.

Medical informatics is an interdisciplinary field. Medical informatics articles will be found in the literature of various disciplines including library and information science publications. The purpose of this study was to provide an objectively ranked list of journals that publish medical informatics articles relevant to library and information science. Library Literature, Library and Information Science Abstracts, and Social Science Citation Index were used to identify articles published on the topic of medical informatics and to identify a ranked list of journals. This study also used citation analysis to identify the most frequently cited journals relevant to library and information science.

Bibliometrics↗

PubMed1998

The Virtual Naval Hospital: a digital library for naval primary care providers and patients.

OBJECTIVE: To deliver optimal patient care and promote wellness, naval providers and patients require convenient access to medical information from isolated duty stations. The Internet can lower barriers to accessing information. The goal of this study was to determine the technical feasibility of using the Internet to deliver medical information to isolated naval points of care. METHODS: A digital library for naval primary care providers and patients with an integrated, problem-based interface was developed. The Virtual Naval Hospital (http:/(/)www.vnh.org) was evaluated from sea, undersea, and shore. RESULTS: The digital library was a popular resource. Access to it is currently limited because few ships have Internet access. CONCLUSIONS: The technical feasibility of delivering a digital library of medical information to the deckplates was proven. The digital library will realize its potential when satellite-based access to the Internet becomes available. In the interim, the digital library will also be distributed on CD-ROM.

CD-ROM↗

PubMed1993

Survey of library and information use by health-care students.

The study compares and contrasts the usage and views of three groups of health-care students (Pre-Project 2000, Project 2000 and BSc Occupational Therapy) on their library facilities. A literature review is included which underpins the importance of libraries for health-care students. Data was compiled from survey responses. The results demonstrate which libraries the students use, how often they use them, what they do in the libraries, and the stock used. They also show the level of student confidence and the services used. The implications of the study for future provision are considered.

Attitude of Health Personnel↗