Eggshell thinning, chlorinated hydrocarbons, and mercury in inland aquatic bird eggs, 1969 and 1970.
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In the embryonated 4-5 days old chicken egg incubated in the normal vertical position, only the blunt top end, a small part of the egg surface, is covered by the embryo. Soon after rotation of the egg to the reverted vertical position with the blunt end down, the egg shell is pierced and the artificial air space is formed on the pointed end which is far from the region occupied by the embryo. Thus any possibility of lesion is avoided.
A case of cerebral schistosomiasis with a huge calcification and with a long clinical course was reported. A 39-year-old male developed ataxic gait gradually associated with headache and general fatigability. According to the past history, the patient had suffered from severe meningitis with disturbance of consciousness for 2 weeks when he was 10 years of age. Neurological examination revealed positive Romberg's sign and left homonymous hemianopsia. Plain craniogram showed a large calcification occupying the whole right occipital region. This calcification was lobulated in shape and was 11 X 9 X 9 cm in size. CT scan demonstrated calcification and remarkable hydrocephalus. This calcification had no mass effect. Left vertebral angiogram revealed the right parieto-occipital artery to be stretched and slightly displaced toward midline. Hematological analysis disclosed no abnormality except for eosinophilia (10%). Pre-operative diagnosis was an old calcified granuloma of unknown origin. For the purpose of biopsy, a right occipital craniotomy was performed. On reflecting the dura, fibrous adhesion and vascular network were seen between the inner table of the dura and the arachnoid. Subarachnoid space was enlarged and all cortical vessels were narrow in their diameters. By a corticotomy, a whitish-yellow calcified mass was seen in the depth of 2 mm. The yellowish gelatinous content poured out of the mass. A piece of calcification and a small amount of content were taken as specimen. Microscopic study of the calcified tissue showed many egg shells of schistosoma japonica, necrotic tissues and infiltration of small round cells.
In the experiments with 35S-labelled sodium sulfate, sulfate sulfur was found to be used for cysteine synthesis in hens. The 35S label was detected in hen's eggs as well, the subshell membranes possessing the highest specific radioactivity. A dependence of 35S incorporation on the ovule developmental stage was revealed. It was established that a shortage of sulfur-containing amino acids in hens' diet can be partially compensated by sodium sulfate sulfur, which influences the concentration of some metabolites.
The injection of 50 mg/kg bodyweight of a prostaglandin synthetase inhibitor (indomethacin) brought about a delayed oviposition time in birds of three breeds of layers, whereas the injection of 5 mg indomethacin had no effect. The daily ingestion of up to 400 mg aspirin or up to 10 mg indomethacin for three weeks, or 750 mg aspirin or 200 mg indomethacin for two weeks had no effect on egg production, oviposition time, egg-shell water conductance, oviducal prostaglandin synthetase activity or bird behaviour. Prostaglandin synthetase activity was demonstrable in both control and treated birds only in the tubular glands of the shell gland. These observations suggest that prostaglandins play a minor role in normal reproductive activity in the female domestic fowl.
A variation is proposed in the surgical technique employed to treat facial vertical axis excess (Long Face Syndrome). The conventional osteotomy used to treat Le Fort's fracture by means of wide horizontal incisions has been modified. The excessive height of the maxilla is treated by vertical incisions united by tunnels, osteotomy of the posterior wall of the maxillary sinus using the "egg-shell" technique, contention by Champy's plates, and genioplasty to correct the constantly exaggerated height of the mandibular symphysis.
The antigenic properties of adult male and female worms of the nematode Dipetalonema viteae (Filarioidea) were studied by immunocytochemistry. Using sera of rodents infected with this parasite, the binding of antibodies to sections of worms embedded in Epon was assayed by the peroxidase-antiperoxidase (PAP) technique and by light microscopy. In female worms antigen-antibody reactions were located in the uterine fluid, the inner uterus wall, the circumference of the microfilariae and in the egg shell. Male D. viteae showed binding of antibodies in the vesica seminalis and in the vas deferens. Both sexes has also less pronounced antigenic activities in cuticula, muscle and intestine.
Unfamiliar manifestation of pulmonary tuberculosis found in Central and West Africa are presented. These include egg shell calcification in parenchyma and glands, calcification of the diaphragmatic pleura resembling asbestosis, honeycombing and B Kerley's lines with or without complicating bronchiectasis. There should be an index of suspicion of pulmonary tuberculosis even when other typical tuberculous lesions are not associated with these lesions not normally attributed to the disease.
The rate of uptake of H3 tyrosine by unisexual females in vitro was accelerated by 22% when male worm extract was added to the culture medium. Pairing of unisexual females with mature male worms in vitro resulted in a similar acceleration of H3 tyrosine uptake. As tyrosine is avidly taken up by the vitelline gland for the formation of egg shell protein the change in the rate of H3 tyrosine uptake can be employed as a sensitive quantitative criterion for assessing its development following stimulation.
Black duck (Anas rubripes) eggs were collected during 1978 in the Atlantic Flyway. One egg from each of 49 clutches was analyzed for organochlorine compounds and mercury. DDE was detected in 39 eggs, ranging from 0.09 ppm to 3.4 ppm, wet weight. DDE residues were highest in eggs from Delaware, where the mean DDE level was 2.0 ppm. DDT and TDE were present at low levels in only five and four eggs, respectively. PCBs resembling Aroclor 1260 were detected in 24 eggs and ranged from 0.43 ppm to 2.9 ppm. Eggs from Massachusetts and Rhode Island contained an average of greater than 1.0 ppm PCBs, but eggs from Nova Scotia, Pennsylvania, Maryland, and Virginia contained no detectable PCBs. Dieldrin, oxychlordane, and heptachlor epoxide were present in a few samples at low levels. Mercury was detected in 31 eggs, ranging from 0.07 ppm to 0.34 ppm, wet weight. Twenty eggs analyzed for chromium, copper, and arsenic contained averages of 0.64 ppm, 1.7 ppm, and 0.18 ppm, respectively. No geographic pattern was observed in these metal residue levels. Eggshell thickness (0.347 mm) was identical to the pre-1946 norm.
A case of hepatosplenic silicosis complicating a pulmonary silicosis is reported. Numerous calcified splenic nodules seen with conventional radiology, ultrasound or computed tomography, hepatic microcalcifications and "egg shell" abdominal lymph nodes best seen with computed tomography represent the radiologic signs. However, hepatosplenic silicosis can only be confirmed by pathological examination of liver biopsies showing birefringent particles within hyalinized nodules.
The ultrastructural study of the zones (D, E, F1) of a dogfish mature egg-shell gland secreting proteic and glycoproteic material brings evidence of a wide range of secretions. Besides the two types of grain found in each of the two zones D and E (respectively spiralled and heterogeneous) a second type of heterogeneous grain as well as a fourth one composed of tiny dense particles are present in zone E. A fifth type of grain is revealed in zone F1. The Golgi apparatus is undoubtly involved in the formation of F1 grains and of both heterogeneous grains, but seems to be irrelevant to zone E secretion of dense grains. As regards spiralled grains there is no evidence of the first stage of their formation in the mature gland. After this first ultrastructural study, the grains specifically involved in the formation of the collagenic protein cannot yet be positively identified.
The localization of the antigen-antibody reactions on methacrylate embedded sections of an adult male and female of the nematode Dipetalonema vitae by the indirect immunofluorescence test was found to be as follows: female D. vitae: in the egg-shell and circumference of sections of the microfilariae as well as amorphous structures in the vesica seminalis and vas deferens. In both sexes: partially in the somatic muscle cells according to their glycogen content, the interstitial spaces between the muscle cells and the connecting pseudocoelomic cavity. Reference to the possible application of this technique in the immunofluorescence diagnosis of filariasis is made.
Cell-cell interactions in the Drosophila ovary play a crucial role in the establishment of dorsoventral polarity of both the egg shell and the future embryo. Torpedo/DER (top/DER), a homolog of the vertebrate epidermal growth factor receptor, is required for this signaling process in the somatic cells of the ovary. In contrast, gurken (grk), which also functions in this pathway, is required in the germline. We cloned the grk gene and found that it encodes a TGF alpha-like protein. Grk is, therefore, likely to be a ligand of top/DER, activating the receptor during oogenesis. During oogenesis, the grk transcript becomes asymmetrically localized to the dorsal corner of the oocyte. We propose that the dorsal localization of grk RNA results in a spatially restricted ligand that asymmetrically activates the receptor.
One hundred thirty patients with Siberian silicosis occurring under exposure to large amounts of dust at labor in a siberian mine were analyzed for 67 clinical and 122 laboratory data. Eighty-eight of 130 patients are now alive, but 42 have already died. When they started work, 122 of the patients were under thirty years of age. The duration of work was 7 to 12 months for 17 patients, 13 to 18 for 43, and 19 to 24 for 40. Seventy-six of 99 patients were initially diagnosed with lung tuberculosis and 23 with silicosis. Almost all patients have complained of respiratory symptoms such as shortness of breath, cough, sputum, and cyanosis. All of the pulmonary function tests including %VC, FEV1.0/FVC, V25/height, RV, TLC, and DLco showed abnormal values. The chest roentogenograms showed 3 of type 1, 22 of type 2, 55 of type 3, and 124 of type 4. Of 124 type-4, large opacities, 84 were type A, 28 B, and 12 C. Of 416 small opacities, 144 were type P, 191 Q, and 81 R. The complications and secondary changes that appeared with progression of the disease were lung emphysema, hilar and mediastinal lymphnode enlargement, egg shell calcification in lymphnode, and bulla or bleb.
Nutritive value and protective efficiency of new foodstuffs for children were studied. Addition to the nutrition of sodium alginate and egg-shell powder renders it prophylactic properties under conditions of cesium-137 intake.