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At least 1,207 records · Page 67Linked to original sources
PubMed1991

Ultrastructural localization of microtubule-associated proteins (MAP) 1 in cilia of the respiratory tract.

The presence and localization of high molecular weight microtubule-associated proteins of the MAP 1 class in ciliated cells of porcine and rat respiratory tract was studied by immunoblotting and immunoelectron microscopy. Ciliary shafts of the porcine tracheal epithelium were isolated using a method that minimizes contamination of the preparation by other cellular fragments and fat. Immunoblotting with rabbit antibodies to bulk MAP 1 from hog brain clearly revealed the presence of anti-MAP 1-immunoreactive high molecular weight proteins of the MAP 1 size in these preparations. To localize MAP 1 proteins at the ultrastructural level, rat and porcine tracheal epithelia were embedded in LR White and subjected to immunogold electron microscopy. Anti-MAP 1-immunoreactive material was found at ciliary shafts and basal bodies, but not at basal feet or ciliary rootlets. Interestingly, the necklace region between the shaft and the basal body of the cilium was hardly reactive with anti-MAP 1 antibodies. This may indicate a reduced stability of ciliary microtubules in this region and could be an explanation why ciliary shafts in general break more easily there than elsewhere.

Animals↗
PubMed1989

[A method for recording the motor activity of the cilia of ciliated epithelium].

The article deals with the television system which registers the lash movements on the screen of TV monitor. The natural moving activity (frequency of whipping) and lashes' activity after the action of different concentration of surface-active substance Triton-H-305 and potassium chloride were investigated on the example of the epithelium of two-folding molluscum. It observes the following type of lashes reaction: unbroken (continuous) whipping, mechanically induced stopping of whipping ("stop effect"), spontaneous "stop effect", starting of whipping due to mechanic action-specific "sorting" lashes (the last are described for the first time).

Animals↗
PubMed1979

Abnormal cilia.

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Cilia↗