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High-performance liquid chromatography of flavonoids in Crataegus oxyacantha L. IV. Reversed-phase high-pressure liquid chromatography in flower, leaf and bud extractives of Crataegus oxyacantha L.

RP-HPLC has been used for qualitative and quantitative analysis of some flavonoids in flowers, leaves and bud extracts of Crataegus oxyacantha L. Peak identification was obtained by comparison with retention times of pure standard substances and by UV analysis. Luteolin, luteolin-3', 7-diglucoside, apigenin, apigenin-7-O-glucoside and rutin have been identified and determined.

Chromatography, High Pressure Liquid↗

[Rapid microdetermination of 2 aminoglycosides by high-performance liquid chromatography in the plasma, bile and urine. Correlation of high-performance liquid chromatography and fluorescence polarization for plasma assays].

An HPLC method for determination of dibekacin and amikacin in various biological samples is described. A step gradient elution and fluorescence detection carried out through an on line post column derivatization system with OPT is used. The chromatographic conditions allow simple and rapid preparation, so that a result can be obtained within twenty minutes. The small volume required (150 microliters) and the rapid sample clean-up are advantageous for routine use even in pediatric patients. The good sensitivity (dibekacin: 0,44 mumol/l - amikacin: 0,35 mumol/l), reproducibility and specificity indicate it to be suitable for pharmacokinetic studies. Results obtained from HPLC were in excellent agreement with those of FPIA for both dibekacin and amikacin (r greater than or equal to 0,98).

Amikacin↗