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Longevity in military pilots: 37-year followup of the Navy's "1000 aviators".

The 37-year nonmilitary mortality rate for initially healthy aviators was determined in a followup program on the U.S. Navy's "1000 Aviator" cohort. Of the 800 survivors of World War II and the Korean conflict, 95 were found to have died from nonmilitary causes over this followup period. This is markedly less than the 208 that would be expected from a random sample of white American men over a similar period (p less than 0.005). It is also significantly less than the 143 that would have been expected from a group of men who had passed an initial insurance physical (p less than 0.005). Lower-than-expected death rates occurred in all three major categories of cause of death in this age group: cardiovascular, neoplastic, and accidental. The generally good socioeconomic background, the positive genetic influence of long-lived parents, the above average intelligence, and the health and fitness orientation of the military aviator are all thought to be factors contributing to this increased longevity.

Accidents↗

Effects of temperature on development of Onchocerca volvulus in Simulium ochraceum, and longevity of the simuliid vector.

The effects of temperature on the development of Onchocerca volvulus in Simulium ochraceum, and on the vector's longevity were studied under simulated environmental conditions in the laboratory. When S. ochraceum (which had fed on a person infected with O. volvulus) were maintained at different, constant temperatures (10-30 C), larvae developed to the infective stage between 20 and 28 C. The time required for development to the infective stage depended on the relationship of y = -0.3760 + 0.0222x (y = velocity in development; x = rearing temperature). However, larval development to the infective stage occurred in all groups kept at different temperatures by day and by night (25 C during the day and 10-18 C during the night), although the developmental period was prolonged (10 days in the group held at 25/18 C, and 13 days in other groups). The percentage of females harboring infective larva(e) among those maintained was highest at a constant temperature of 22 C (21.9%), followed by 15.0% at 25 C. When S. ochraceum females were held at day/night differing temperatures, much higher rates were observed at 25/16 C (30.0%) and at 25/14 C (23.0%). Our results suggest that the distribution of onchocerciasis in Guatemala may be related to ambient temperature and to day/night temperature cycles.

Animals↗

Longevity and productivity of Taenia taeniaeformis in cats.

The longevity and productivity of Taenia taeniaeformis were studied in experimentally infected cats. Nineteen of 20 cats became infected after they were given 8 to 12 strobilocerci. In 8 cats, mean prepatent period was 47.1 days +/- 5.9 SEM (34 to 80 days). Patent periods in the infected cats were 7 to 34 months. In 11 cats in which the infections were allowed to terminate naturally (spontaneous recovery), mean patient period was 17.4 months +/- 2.3. Two of these cats were then given a 2nd dose of strobilocerci and both became reinfected. The mean daily proglottid output in 6 cats followed throughout the patent period was 4.3 +/- 0.5. Destrobilization occurred spontaneously and sporadically throughout the infection, but did not always increase at the time of natural termination. Usually, proglottid productivity began to decrease after the 1st year of infection. Shed proglottids contained 0 to 12,180 eggs, with a mean of 1,606 +/- 402, but more than 60% of the segments contained 500 or less. Taenia taeniaeformis appeared to be as persistent as other taeniids, such as T ovis and T hydatigena in the dog, but it was far less prolific as an egg producer.

Animals↗

Stimulated autoantibody response and increased longevity in NZB/NZW mice treated with cyclophosphamide and tilorone.

The experiment described in this report was designed to study the effects of immunostimulatory therapy in cyclophosphamide-treated hybrid New Zealand mice. Autoantibodies, renal histology and neoplasms were studied in seventeen female NZB/NZW mice treated with daily injections of the potent immunosuppressive drug, cyclophosphamide. Results were compared with fifteen female NZB/NZW mice who received both cyclophosphamide and tilorone, an interferon inducer which stimulates the immune system. Fifteen control mice received saline. The controls died with spontaneous arteritis and immune complex glomerulonephritis; their mean age at death was 46 weeks. In the cyclophosphamide group anti-DNA antibodies and renal disease were suppressed. Mean longevity was prolonged significantly to 80 weeks. Two mice died of iatrogenic causes, and the remaining fifteen mice died with neoplasms. Eleven mice had multiple neoplasms; a total of twenty-seven neoplasms appeared. In mice receiving combination therapy, autoantibody responses were not suppressed. Nevertheless, glomerulonephritis was controlled partially and the mean lifespan was prolonged to 82 weeks. Eighteen neoplasms appeared in ten mice in the combination treatment group, and five mice had more than one neoplasm. The appearance of lymphomas was delayed in mice receiving two drugs. It was concluded that concurrent therapy with tilorone stimulated autoantibody production and altered the expected pattern of neoplasia in cyclophosphamide-treated NZB/NZW mice.

Animals↗

[Prognosticated longevity quotient (PLQ) and the assessment scale for older patients].

In a previous article was demonstrated that the physician is over-optimistic while making an individual prognosis as to survival in patients of a psychogeriatric nursing home. In this investigation we have tried to relate the individual prognosis to the BOP (a Dutch derivative of the Stockton Geriatric Rating Scale). We introduced the term Prognosticated Longevity Quotient: the prognosticated survival in years divided by the expectation of life in Netherlanders of the same age and sex. In the 217 patients, prospectively studied, an inverse relation was found between PLQ- and BOP-values. At the end of the 45-months lasting investigation period, the initial PLQ turned out to have been lower and the BOP higher in those who had died compared to those who survived. Chances of being alive were greater in those with an initial PLQ > 0,5 while initial placement in an increasingly higher BOP-class indicated a decreasingly smaller chance of survival.

Age Factors↗

Poly(ADP-ribose) polymerase activity in intact or permeabilized leukocytes from mammalian species of different longevity.

Poly(ADP-ribosyl)ation is a eukaryotic posttranslational protein modification catalyzed by poly(ADP-ribose) polymerase (PARP), a highly conserved nuclear enzyme which uses NAD as substrate. We have previously tested PARP activity in permeabilized mononuclear blood cells (MNC) from 13 mammalian species as a function of the species-specific life span. A direct and maximal stimulus of PARP activation was provided by including saturating amounts of a double-stranded oligonucleotide in the PARP-reaction buffer. The data yielded a strong positive correlation between PARP activities and the species' maximal life spans (r = 0.84; p << 0.001). Here, we investigated the formation of poly(ADP-ribose) in living MNC from two mammalian species with widely differing longevity (rat and man) by immunofluorescence detection of poly(ADP-ribose). The fraction of positive cells was recorded, following gamma-irradiation of intact MNC, as a semiquantitative estimation of poly(ADP-ribose) formation. Human samples displayed a significantly higher percentage of positivity than did those from rats, consistent with our previous results on permeabilized cells. While rat MNC had a higher NAD content than human MNC, the number of radiation-induced DNA strand breaks was not significantly different in the two species. Since poly(ADP-ribosyl)ation is apparently involved in DNA repair and the cellular recovery from DNA damage, we speculate that the higher poly(ADP-ribosyl)ation capacity of long-lived species might more efficiently help to slow down the accumulation of unrepaired DNA damage and of genetic alterations, as compared with short-lived species.

Animals↗

Changes in physical activity and other lifeway patterns influencing longevity.

We studied the adoption or maintenance of physical activity and other optional lifeway patterns for their influence on mortality rates of Harvard College alumni. Men aged 45-84 in 1977, surveyed by questionnaire in 1962 or 1966 and again in 1977, were followed from 1977 through 1988 or to age 90. Of 14,786 alumni, 2,343 died in 165,402 man-years of follow-up. Relative risks of death, standardized for potential confounding influences, for men who between questionnaires increased their physical activity through walking, stair climbing, and sports or recreational activities to 1,500 kcal or more per wk were 0.72 (95% confidence interval 0.64-0.82), compared with 1.00 for men who remained less active. Corresponding relative risks for men who adopted moderately vigorous sports play (> or = 4.5 METs) were 0.73 (0.65-0.81) vs 1.00 for men not adopting such sports; and for cigarette smokers who quit, 0.74 (0.65-0.84) vs 1.00 for persistent smokers. Men with recently diagnosed hypertension had a lower death risk than long-term hypertensives (0.80; 0.70-0.92), as did men with consistent normotension (0.52; 0.47-0.58). Changes in body-mass index had little influence on mortality during follow-up. These findings fit the hypothesis that adopting a physically active lifeway, quitting cigarette smoking, and remaining normotensive independently delay all-cause mortality and extend longevity.

Adult↗

Worklives and longevity: insights from a life course perspective.

Recent research on work stress has focused heavily on how the demands and rewards of single jobs influence health or mortality, but has left the implications of broader patterns of worklives largely uninvestigated. Life history data from the Stanford-Terman study is used in this research to explore patterns of career mobility among middle-class men and the implications of these patterns for their longevity. While relatively few men in this study experienced a period in which they moved through a series of unrelated jobs, those who did had a higher mortality risk. Similarly, men who progressed early in their careers but then remained stable in later periods tended to be at a greater risk than those who progressed in both time periods. Health and lifestyle measures were also significant predictors of mortality risk, but did not account for the observed impact of worklife patterns on mortality. We discuss the implications of these findings for understanding the work-health relationship.

Adult↗

[Interrelation of genetic dimorphism of ear wax and the level of apolipoproteins with atherogenesis and longevity in the Lithuanian population].

Genetic dimorphism of the cerumen was studied in a random sample from the Lithuanian population (N = 253), among the patients with the most atherosclerotic risk, from different age groups (N = 276) and in a cohort of long-living (N = 117). Simultaneously, the levels of apolipoprotein (apo- A-1, B, E) were determined in blood sera of the males-donors, long-living and elderly individuals depending on the phenotypes for ear wax consistency. The prevalence of frequency of the w gene for humid cerumen in children suffering from the insulin-dependent diabetes mellitus was found as compared with populational sample--0.8293 and 0.6024, respectively (P < 0.002). On the contrary, essential increase in frequency of the d gene responsible for dry cerumen in long living was found as compared with the control--0.5311 and 0.3976, respectively (P < 0.01). An absence of differences in the concentrations of w and d alleles among the patients with coronary artery atherosclerosis and the populational control indicated that the genetic characters under study exerted no marked change in incidence of atherogenesis. However, the ratio apoB/apoA-1 proved higher in the donors with humid ear wax than in those with the dry variant under P < 0.06, which can stimulate this disease. The results of this study support the statement that low level of apoB and especially apoB/apoA-1 may be one of the longevity markers.

Adolescent↗

Effect of temperature and humidity on longevity of unfed adults and on oviposition of engorged females of Dermacentor reticulatus (Ixodidae).

The effect of ambient temperature and relative humidity on longevity of unfed adult ticks and on oviposition of engorged female ticks of Dermacentor reticulatus was examined. The 50% mortality time of unfed adult ticks, incubated at 5 degrees C, 10 degrees C, 20 degrees C and 27 degrees C and humidities of 15%, 50% and 100%, increased irrespective of their sex with rising relative humidity and decreasing temperature. It was longest at 5 degrees C and 100% r.h. being 617.8 days and shortest at 27 degrees C and 15% r.h. amounting only to 33.6 days. All unfed ticks survived an incubation of 150 days at -10 degrees C and 15%, 50% and 100% r.h. as well as an exposure of 180 days at 0 degrees C and 100% r.h. At 0 degrees C the percent survival was 75% for the males and 40% for the females at 50% r.h. but 100% for males and 95% for the females at 15% r.h. after exposure of 180 days. Oviposition of engorged females occurred at a temperature range of 10-27 degrees C, at 10 degrees C merely at 50% and 100% r.h. The percentage of ovipositioning females amounted to at least 80% apart from 10 degrees C and 50% r.h. being only 10%. The egg index and the egg conversion index as well as the larvae index and the larvae conversion index generally increased with rising temperature and relative humidity. The indices were lowest at 10 degrees C and 50% r.h. and were highest at 20 degrees C and 27 degrees C at 100% r.h. The reproduction capability of engorged female ticks, held at -10 degrees C, 0 degrees C and 5 degrees C and 15%, 50% and 100% r.h. and transferred to 20 degrees C and 95% r.h., persisted for 10 weeks at -10 degrees C and for 2 weeks at 0 degrees C at the most, but for at least 20 weeks at 5 degrees C. There were no significant differences of the preoviposition periods, egg indices and larvae indices as well as of the minimum embryonic developmental times and hatching rates with regard to the preceding incubation conditions and the length of the ticks' exposure.

Animals↗

[Longevity of Trypanosoma cruzi in the Triatoma infestans].

Maintenance of Trypanosoma cruzi infection in Triatoma infestans was evaluated. Seventy nine fourth instar nymphs were followed by 12 months, after being fed in infected mice. The bugs were examined every month, fecal smears were done to document T. cruzi infection then the bugs were fed in "clean" (uninfected) mice. Thirty nine per cent of the bugs remained infected until death twenty three per cent cleared infection and remained uninfected until death and nineteen per cent alternated positive and negative results. Our experience agrees with the published papers in medical literature. Longevity of T. cruzi in the host depends mostly in the feeding of the bug; well fed bugs maintain infection, and long fasting correlates with infection clearance.

Animals↗

[Demographic factors of longevity in Siberia and Far East].

Longevity indexes LI2 and LI3 (subjects aged 90 and more and 100 and more, respectively, among subjects of 60 and older) were compared in all administrative units on the basis of results of 1989 Census of the population and data of the Central Statistical Office. These values reduce from West to East: LI2 in Western Siberia is equal to 8.48/1000, LI3 to 0.45/1000, in Eastern Siberia these values are 7.48 and 0.35/1000, in the Far East 5.20 and 0.31/1000, in Russia on the whole 9.16 and 0.47/1000, respectively. The territories with relatively high LI2 values were the Aghinsk Buryat Autonomous Territory (11.42/1000), Yakutia (10.79/1000), Altai (9.41/1000).

Aged↗

[Growth and longevity of Wistar rats receiving a diet whose protein content is rapidly lowered in relation to aging].

Growth and longevity of the Rats were the same when: (i) the diet was rich in casein (21%), from weaning to death: (ii) the casein content of this diet (without B12) was progressively lowered from weaning to 200 days of age, and thereafter was 7%. When the casein level had been too rapidly lowered, inducing retardation of growth after 75 days, maximal growth was lowered and life span was increased.

Aging↗

[Why regular physical activity favors longevity].

Regular physical exercise is useful at all ages. In the elderly, even a gentle exercise programme consisting of walking, bicycling, playing golf if performed constantly increases longevity by preventing the onset of the main diseases or alleviating the handicaps they may have caused. Cardiovascular diseases, which represent the main cause of death in the elderly, and osteoporosis, a disabling disease potentially capable of shortening life expectancy, benefit from physical exercise which if performed regularly well before the start of old age may help to prevent them. Over the past few years there has been growing evidence of the concrete protection offered against neoplasia and even the ageing process itself.

Age Factors↗

The longevity of a bilayered skin substitute after application to venous ulcers.

BACKGROUND: A bilayered skin substitute composed of allogeneic keratinocytes and fibroblasts in a collagen gel has been approved by the US Food and Drug Administration for the treatment of venous and diabetic ulcers. Its mechanism of action has not been fully determined. OBJECTIVE: To determine the longevity of allogeneic fibroblasts and keratinocytes in a bilayered skin substitute in patients with venous leg ulcers. METHODS: Ten patients with venous leg ulcers were treated with a bilayered skin substitute on day 0, days 3 to 5, and weeks 1 through 3. Biopsy specimens of the grafted wound were taken. We used polymerase chain reaction analysis to determine whether allogeneic DNA was present in the biopsy specimens. RESULTS: We detected allogeneic DNA in 2 of 8 specimens at 1 month after initial grafting. Neither of the 2 patients showed persistence of allogeneic DNA at 2 months after initial grafting. CONCLUSIONS: Allogeneic cells from a bilayered skin substitute do not appear to survive permanently after grafting for treatment of venous leg ulcers. Other mechanisms of action might include cytokine release, structural support, or provision of a moist wound environment.

Collagen↗

Combined effect of cyclosporine and sirolimus on improving the longevity of recombinant adenovirus-mediated transgene expression in the retina.

OBJECTIVES: To reevaluate the longevity and intraocular safety of recombinant adenovirus (rAd)-mediated gene delivery after subretinal injection, and to prolong transgene expression through the combination of 2 synergistic immunosuppressants. METHODS: An rAd vector carrying green fluorescent protein (GFP) gene was delivered subretinally in the rat eye. The GFP expression was monitored in real time by fundus fluorescent photography. Intraocular safety was examined by observation of changes of retinal pigmentation, cell infiltration in virus-contacted area, immunophenotyping for CD4(+) and CD8(+) cytotoxic T lymphocytes, and CD68(+) macrophages, histologic findings, and dark-adapted electroretinography. Two synergistic immunosuppressants, cyclosporine and sirolimus, were used alone or in combination to prolong transgene expression by temporary immunosuppression. RESULTS: The GFP expression peaked on day 4, dramatically decreased on day 10, and was not detectable on day 14. The decreased GFP expression was coincident with cell infiltration in virus-contacted area. Immunostaining showed that the infiltrating cells were CD4(+) and CD8(+) cytotoxic T lymphocytes and CD68(+) macrophages. Clumped retinal pigmentation and decreased b wave of dark-adapted electroretinogram were observed at 3 to 4 weeks after injection. Histologic examination confirmed rAd-induced retinal degeneration. Transient immunosuppression by cyclosporine and sirolimus, either alone or in combination, improved transgene expression, with the combination being the most efficient. The combined immunosuppression attenuated but did not retard the rAd-induced retinal damage. CONCLUSIONS: Transgene expression mediated by rAd after subretinal delivery is short-term and toxic to the retina. Combination of cyclosporine and sirolimus may act as an immunosuppressive adjunct to prolong rAd-mediated gene transfer. CLINICAL RELEVANCE: The intraocular safety of rAd should be carefully considered before clinical trials are performed.

Adenoviridae↗

Intravitreal longevity of three perfluorocarbon gases.

Three gases, perfluoromethane, perfluoroethane, and perfluoropropane (CF4, C2F6, and C3 F8, respectively) were studied for their possible value as intravitreal tamponades in retinal surgery. The gases expand and have intraocular longevities that increase with the length of the carbon chain. A 0.4-cc injection of CF4, remained in the eye six days, twice as long as an equivalent amount of air; C2F6 remained 16 days, more than four times as long as air; and C3F8 remained 28 days, nine times as long as air and four times as long as sulfur hexafluoride.

Air↗

High incidence of supernumerary nipples and twins in formosan macaques (Macaca cyclopis) at Mt. Longevity, Taiwan.

A population of Formosan macaques at Mt. Longevity exhibits an unusually high incidence of supernumerary nipples (polythelia: between 1-6 accessory nipples and/or areolae on 33% of adults), as well as a high rate of twinning (about 1% of births). The coexistence of these unusual traits suggests a connection, which is further supported by a tendency for mothers of twins to have accessory nipples and for twins to be born in troops with high incidence of polythelia.

Animals↗