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At least 1,189 records · Page 66Linked to original sources
PubMed2005

Construction of a human Fab phage display library from antibody repertoires of osteosarcoma patients.

Osteosarcoma is the commonest type of primary malignant bone tumor, frequently found in adolescents at sites of rapid bone growth. Despite current management protocols, up to half of the patients succumb to this disease. Moreover, there is no well-characterized molecular marker for diagnosis and prognosis. Since phage display methodology allows the selection of human antibody fragments with potential use in clinical applications, we applied this procedure to construct a recombinant Fab (antigen binding fragment) library from patients with osteosarcoma. We used peripheral blood lymphocyte total RNA from 11 osteosarcoma patients and cloned recombinant Fab representing the micro, gamma and kappa chain antibody repertoires of these individuals. The resulting library was cloned in the pComb3X vector and attained 1.45 x 10(8) different functional forms. BstO I fingerprinting and DNA sequencing analysis of randomly selected clones revealed the diversity of the library, demonstrating that Fab harbors Vkappa chains from subgroups I to V, biased towards the A27 fragment, as normally reported for the human repertoire. Analysis of the VH repertoire revealed that our library has a slight bias towards the VH4 family, instead of the usually reported VH3. This is the first description of a phage display library from osteosarcoma patients. We believe these human Fab fragments will provide a valuable tool for the study of this neoplasia and could also contribute to improvements in the diagnosis of this disease.

Adult↗
PubMed2005

[Screening of prothrombotic state-related genes from human hepatic cDNA library].

OBJECTIVE: To screen prothrombotic state-related cDNA sequences from human hepatic cDNA library. METHODS: The subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state (PTS) was constructed by suppression subtractive hybridization. Positive clones were identified by differential screening. The target DNA sequences of positive cDNA clones of differentially expressed genes from rat liver of PTS were amplified by PCR. The PCR products were used as probes to screen a human hepatic cDNA library. After the first, second and third screening, the transduction of a positive lambdaTripIEx lysate into E. coli strain BM25. 8 promoted the circularization of pTripIEx. The positive circularized plasmids were identified by double enzyme digestion. The cDNA fragments of the positive plasmids were sequenced and analyzed by bioinformatics (blastn). RESULTS: Four PTS-related cDNA sequences were identified from human hepatic cDNA library. For 3 of them, their products of expression were respectively fibrinogen, LFIRE1, and chromosome 10 open reading frame 104 mRNA. The fourth sequence had homology with carbamoyl-phosphate synthetase 1 gene. CONCLUSION: Four PTS-related cDNA sequences have been identified from human hepatic cDNA library.

Base Sequence↗
PubMed2006

[Construction of subtracted cDNA library by suppression subtractive hybridization for differentially expressed genes in eosinophils from asthma patients].

OBJECTIVE: To construct a subtracted cDNA library of differentially expressed genes in eosinophils from asthma patients. METHODS: Suppression subtractive hybridization (SSH) was used to isolate the cDNA fragments of differentially expressed genes in the eosinophils of asthma patients before and after treatment. The cDNA fragments were directly inserted into T/A cloning vector to establish the subtractive library, followed by amplification of the library through E. coli transformation with calcium chloride and screening of blue and white clones of the transformants. One hundred positive bacterial clones were randomly picked and identified by colony PCR. RESULTS: The amplified library contained more than 3,000 positive bacterial clones. Analysis of the randomly selected 100 white clones by PCR showed that 90% of the clones contained 100-500 bp inserts, which might be the cDNA fragments of differentially expressed genes in eosinophils of asthma patients before treatment. CONCLUSION: A subtracted cDNA library of differentially expressed genes in the eosinophils of asthma patients before and after treatment is constructed successfully by SSH and T/A cloning techniques, which lays a solid foundation for screening and cloning new specific differentially.expressed genes in the eosinophils of asthma patients.

Asthma↗
PubMed2006

[Construction and primary identification of a naive Fab fragment phage library].

OBJECTIVE: To construct a naive human Fab fragment phage display library, provide a platform for human antibody preparation and a new therapy for the malignant tumors. METHODS: Peripheral blood lymphocytes were isolated from 200 ml blood, which was obtained from a healthy blood donor. The heavy chain Fd fragments and light chain cDNA synthesized from the total RNA of lymphocytes were amplified by PCR and the amplification products were ligated into the phagemid vector pComb3, then the ligated sample was transformed into competent E. coli XL1-Blue. The transformed cells were infected with VCSM13 helper phage to yield recombinant phage antibody of Fabs. The phagemids abstracted from amplified E. coli were cut with endonucleases such as Sac I, Xba I, Spe I and Xho I and amplified by PCR to monitor the insertion of the light chain or heavy chain Fd genes. Human albumin and interleukin-2 were utilized as antigens to conduct respectively three rounds of panning to the original Fab antibody library. RESULTS: By combination of light chain and heavy chain genes, an antibody library containing 1.2 x 10(6) clones was obtained, and both the cutting of enzymes and PCR showed that there were the light chain or heavy chain Fd genes of the phagemids. After the original antibody library having been panned respectively by two kinds of antigen proteins, it gained enrichment in different degree. CONCLUSION: Utilizing the technology of phage surface display, special antibody can be gained from the human naive Fab phage display library, which can be used as a new therapy for tumors.

Antibodies↗
PubMed1991

The establishment of an academic health sciences library in a developing country: a case study.

The development of a Faculty of Medical Sciences (FMS) and an academic health sciences library for the University of the West Indies (UWI) has proven to be a polemical and political issue due to the depressed economy of the country. Although FMS is still shrouded in politics and controversy after its inaugural year, the Medical Sciences Library (MSL) has expanded its dimensions and is actively developing a biomedical information network within the country. This will result in better dissemination and control of biomedical information. The library now participates in joint projects with other health sciences libraries in the country with the goal of joint automated listings of holdings and shared cataloging projects. This paper examines the development of the library and explains the difficulties experienced in its developmental stages due to politics, the delay in appointment of a medical sciences librarian, and the financial decline in the local economy.

Developing Countries↗
PubMed1988

Requests for proposals for library automation.

Many health sciences libraries are now considering integrated automated systems for an investment of several hundred thousand dollars. The request for proposals (RFPs) is the usual method of selecting candidate vendors for close inspection of promising systems. This paper draws upon the experience of the Library of Rush University and that of twelve other health sciences libraries. The libraries were interviewed by telephone using a short questionnaire that asked them to compare the RFPs with the systems actually obtained and their experiences in implementing them. The libraries were also asked, with the benefit of hindsight, what would they have done differently. Four vendors were also interviewed by telephone, in order to get their point of view.

Chicago↗
PubMed1986

The AHEC library program and consortia development in California.

A brief history of the first Area Health Education Center (AHEC) Library Program in California is presented, with a description of methodology and results. The goals of this program were to develop and improve hospital library resources and services, to train hospital library personnel, and to promote resource sharing in a medically underserved area. The health sciences library consortium that evolved became a model for the ten other library consortia in the state. Based on AHEC's twelve years' experience with consortia, from 1973 to 1985, recommendations are made as to size, composition, leadership, outside funding, group participation, publicity, and linkages.

Area Health Education Centers↗
PubMed1979

Library school education for medical librarianship.

This paper reviews the current situation in library school education for medical librarianship in the United States and Canada based on information from a questionnaire sent to teachers of courses in medical librarianship in accredited library schools. Since 1939, when the first course devoted entirely to medical librarianship was offered at Columbia University, courses have been introduced into the curricula of at least forty-seven of the ALA-accredited library schools. In 1978 there were seventy courses available through forty-seven library schools. Possibilities for specialization in medical librarianship are examined. Course content is reviewed. Implications of the MLA certification examination for library school courses are explored.

Certification↗
PubMed1973

Computer information project for monographs at the Medical Research Library of Brooklyn.

The article describes a resource library's computer-based project that provides cataloging and other bibliographic services and promotes greater use of the book collection. A few studies are cited to show the significance of monographic literature in medical libraries. The educational role of the Medical Research Library of Brooklyn is discussed, both with regard to the parent institution and to smaller medical libraries in the same geographic area. Types of aid given to smaller libraries are enumerated. Information is given on methods for providing machine-produced catalog cards, current awareness notes, and bibliographic lists. Actualities and potentialities of the computer project are discussed.

Bibliographies as Topic↗
PubMed1968

The Veterans Administration library program.

The Veterans Administration Library Service is continuously responsive to the information requirements of the agency's policies which provide for the improved care and treatment of veterans through research, education, and clinical programs. At the same time, it participates in the planning of the federal government as a whole in providing library support for health care for the American people. There are both internal and external forces influencing VA hospitals and their libraries. Retirements and consequent recruitment of new people will necessitate a rethinking of the VA library program at the same time as external forces will be affecting the program. These external forces include the application of machines to library services through the development of in-house capabilities coupled with joint-use participation and P.L. 89-785 which provides for the exchange of medical information, sharing of facilities, and cooperative training programs. A conceptual rearrangement of information resources will facilitate attainment of our goals.

Libraries, Medical↗
PubMed1968

The changing role of the library.

This article reviews the extraordinary growth in the scientific literature that has resulted from increased federal expenditures in the past decade or two. The article further notes that the impact of the knowledge explosion has impinged on the Health Sciences Library as well as on the individual scientist who needs access to the information. A strong plea is made for the librarian to assume a more active role in: doing internal research with respect to how best to use the library as a tool in the dissemination of new information; educating newcomers to the field of library science with respect to the management of scientific information; and converting the library from a passive to an active instrument in disseminating the scholarly record to and among those who require access to it. Medical center administrators are reminded that if the librarian succeeds in these ventures then he will fulfill all of the research, teaching, and service requirements ordinarily made of other academic departments and, in turn, should be rewarded with departmental status for the library.

Information Services↗
PubMed1970

An overview of RMP library activities: present and future.

The creation of the Regional Medical Programs has resulted in the origin of many library-related projects. Different regions have had a variety of projects. Surveys of resources in hospital libraries, continuing medical education surveys, and workshops for training hospital library personnel have been frequent. Instructional materials for library education have been developed or improved. Reference services have been stressed in many regions, and union lists of serials have been started. Grants for library projects total $1.4 million.

Education, Continuing↗
PubMed1970

The Osler Library: today and tomorrow.

The Osler Library's purpose is to build a collection capable of supporting research in the history of medicine. The major acquisition area is material published from 1960 to 1968. Out-of-print material is acquired on microfilm. The Library contains all pre-1850 monographs, transferred from the Medical Library, and all new monographs from 1850 to 1899 are housed there. The catalogs are described, together with special files, including chronological, journal article index, and publishers' lists index.The place of the original Osler collection is discussed, as are methods by which the collections are preserved. The Library hopes to become a deposit center in Eastern Canada for historical medical material.The administration and organization of the Library are described.

History of Medicine↗
PubMed1968

The total library.

Changing functions and techniques of today's libraries have led to questioning the very substance of the library of the future. Ralph Shaw points out that the total library must be "a living force for the enrichment of mankind." The Medical Research Library of Brooklyn at its very inauguration is uniquely prepared and equipped to work toward that goal. It imaginatively serves the needs of the immediate area by generous sharing of resources, use of its computerized program, and participation in a state-wide system. The large collection of the Academy of Medicine of Brooklyn offers thousands of volumes for the historian, both great works as highlights of medical achievements and more modest contributions of both early and recent date. The total library must serve as an intellectual resource, as well as a mechanism for the rapid transfer of current information.

Academies and Institutes↗
PubMed1969

Role of the hospital library within the hospital system.

The results of a survey of hospital administrators, attending staff and house staff physicians, librarians, library committee chairmen, and nursing staff in five Northeastern Ohio hospitals concerning the status of the hospital library within the total hospital system are related. Results indicate that hospital libraries operate in the "fringe" area of the hospital system. A concentric-circle figure indicates the present position of the majority of hospital libraries surveyed. The future relationship of the library within the hospital system has also been represented by a concentric-circle figure.

Hospital Administration↗
PubMed1969

Postage--boon or bane: an evaluation of one library's MLA exchange transactions.

An analysis of MLA Exchange material shipped as well as received is presented. It was found that, of 10,382 items shipped during a twelve-month period to 588 libraries, 332 of these mailings, or 56 percent, were sent for ten cents or less, at a total of $24.80 for postage, an average of $6.20 per exchange list. Similarly, a six-month log of items received by the library shows that 55 percent of these mailings was sent to the library for ten cents or less. The results of this study suggest that the waiving of small fees could eliminate more than half of the donor library's record keeping on postal reimbursements and that it could cut the receiving library's actual reimbursement cost, which is shown to reach 250 percent.

Costs and Cost Analysis↗
PubMed1969

The library committee in the medical school.

The value of the library committee, commission, or council has been debated by librarians in various types of libraries. A questionnaire of forty-one questions was sent to 109 medical and osteopathic school librarians. Ninety-nine respondents answered pertinent questions. These questions attempted to ascertain the actual functions and accomplishments of the faculty library committee. Librarians were encouraged to express their views on how well the committee fulfilled its functions and whether they thought the committee to be an asset. Although this study is based on the experience of medical school librarians, the literature on the library committee in university, college, public, school, special, and medical school libraries is reviewed.

Libraries, Medical↗
PubMed1976

Physical facilities of medical school libraries in the United States, 1966-1975: a statistical review.

This paper, based on information received from a questionnaire to which 107 of the present 114 medical school libraries in the U.S. responded, shows the vast growth and expansion of such libraries. The 86 libraries which have been built, expanded, under construction of planned during 1966-1975 represent the greatest expansion in the history of medical school libraries. This "decade of revolution" can be attributed to the evergrowing resources, primary users, and services discussed in this paper. The survey results can also be useful to those institutions planning or remodelling such library facilities in the near future.

Architecture↗