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[Spontaneous corrections, growth disorders and post-traumatic deformities after fractures in the area of the forearm of the growing skeleton].

Growth phenomena after paediatric forearm fractures are described. The capacity for spontaneous remodelling of malunions should be primarily considered in the treatment of fractures of the growing skeleton. Thus, unnecessary reductions, anaesthesia and posttraumatic deformities can be prevented. Generally speaking, a high remodelling capacity can be expected in cases of enough remaining growth, proximity to a physis with high activity, and if the main deformity lies in the plane of motion of the nearest joint. It is widely accepted that distal radius and/or ulna fractures are fully remodelled up to the age of 11 to 12 years. However, the remodelling capacity of fractures of the proximal and middle third of the shaft is smaller and less well known. Stimulating growth disturbances at the upper extremities are clinically of minor importance. Growth arrests are rare. Their fateful occurrence is not predictable and not closely related to fracture pattern or amount of dislocation.

Bone Remodeling↗

Sipandinolide: a butenolide including a novel type of carbon skeleton from Siparuna andina.

From a lipophilic extract of leaves of Siparuna andina (Monimiaceae), which exhibited antiplasmodial activity in vitro, two new compounds have been isolated: sipandinolide (1), a compound with a novel type of carbon skeleton and (-)-cis-3-acetoxy-4',5,7-trihydroxyflavanone (2). Their structures were established by spectroscopic methods; 2 showed moderate antiplasmodial activity whereas 1 was inactive.

Animals↗

Antifungal metabolite with a new carbon skeleton from Keissleriella sp. YS4108, a marine filamentous fungus.

In addition to four known metabolites (4-acetyl-6,8-dihydroxy-5-methylisocoumarin, 6,8-dihydroxy-3-methylisocoumarin, 6,8-dihydroxy-3,5,7-trimethylisocoumarin and 3,3'-oxy-(5-methyl)-phenol), bioassay-guided fractionation of the culture of Keissleriella sp., a marine filamentous fungus (strain number: YS 4108), afforded an antifungal metabolite with a new carbon skeleton whose structure was elucidated spectrometrically as 3,6,8-trihydroxy-3-[3,5-dimethyl-2-oxo-3(E)-heptenyl]-2,3-dihydronaphthalen-1(4H)-one. In vitro antifungal assays of all isolates revealed that the new metabolite and 3,3'-oxybis[5-methylphenol] were inhibitory to the growth of the human pathogenic fungi Candida albicans, Tricophyton rubrum and Aspergillus niger with MICs of the former being 40, 20 and 80 microg/ml, and those of the latter 10, 30 and 50 microg/ml, respectively.

Antifungal Agents↗

Non uniform effect of estradiol on the rat skeleton.

Pharmacological doses of estradiol administered to rats increased the Ca mass of their femurs through a significant reduction in the rate of Ca resorption. On a body basis, the same animals showed increased rates of skeletal Ca deposition and resorption and no effect on the skeletal mass. The non uniform effect of estradiol on the skeleton, if confirmed in the human being, may limit the applicability of estrogenic treatment.

Animals↗

Ultrasound analyses of the calcaneus predict relative risk of the presence of at least one vertebral fracture and reflect different physical qualities of bone in different regions of the skeleton.

Broadband ultrasound attenuation (BUA) and speed of sound (SOS) were determined together with bone mineral density (BMD) of the lumbar spine and of the proximal right hip (QDR 2000, Hologic) in 1252 females (mean age 56 +/- 11). We then calculated the predictive power of BUA and SOS for at least one vertebral fracture and correlation-coefficients of BMD of the spine and hip as well as BUA and SOS in different age groups (SPSS PC + Vers. 2.2. for IBM PC software). The overall prevalence of at least one vertebral fracture was 10.14% (127 out of 1252 patients). The relative risk for the presence of at least one vertebral fracture was 2.7 for BMD spine below 0.7 g Calcium/cm2 (69 out of 249 patients), 1.8 for BMD hip below 0.7 g Calcium/cm2 (106 out of 589 patients), 1.9 for SOS calcaneus below 1500 m/s (99 out of 499 patients) and 1.7 for BUA calcaneus below 110 dB/MHz (103 out of 605 patients). The relative risk for the presence of at least one vertebral fracture increases significantly (p < 0.01). In the age group < 50 the coefficients of correlation were 0.4655 between BMD spine and BUA calcaneus, 0.5259 between BMD spine and SOS calcaneus, 0.4327 between BMD hip and BUA, 0.2760 between BMD hip and SOS. In the age group > 70 the coefficients of correlation were 0.3699 between BMD spine and BUA, 0.3481 between BMD spine and SOS, 0.5946 between BMD hip and BUA, 0.5138 between BMD hip and SOS, respectively. All coefficients of correlation were highly statistically significant (p < 0.001). We conclude that BUA and SOS predict relative risk of the presence of at least one vertebral fracture as well as BMD spine and BMD hip. With increasing age BUA and SOS appear to be an independent predictor, not being inevitably correlated to BMD but possibly reflecting different qualities of bone at different sites of the skeleton.

Absorptiometry, Photon↗

[Stress reactions of the bony skeleton of athletes].

A stress fracture atypical lesion caused by overstraining the bony skeleton could result in a long-term rest in training. Depending on the type of stress, the localisation changes. The lower extremities are without doubt afflicted most frequently. Complaints, findings and x-ray findings may be unspecific. This complicates the diagnosis in the same way as stress fractures with rare localisation. The concept "stress fracture" is often misleading and should preferably be replaced by "stress reaction". This applies particularly to non-typical radiological patterns. Diagnosis, differential diagnosis and therapeutical aspects are discussed.

Adolescent↗

[Calcitonin gene products and the skeleton].

Calcitonin is the most important hypocalcaemic peptide. Its action is brought about through inhibition of bone resorption. CGRP exerts the same effect on the skeleton albeit in much higher concentrations probably through interaction with calcitonin receptors on osteoclasts. PAS-57 stimulates the incorporation of thymidine into osteoblasts. A possible stimulation of bone formation needs to be verified in vivo.

Bone Resorption↗

[Nuclear spin tomographic study of the facial skeleton. I. Nuclear spin and computed tomographic anatomy].

The resolution of NMR and CT was compared using axial sections of the normal anatomic structures of the facial skeleton. It was shown that NMR was superior in differentiating muscles, tonsils, mucosa, lymph nodes and blood vessels. The demonstration of bone and its differentiation from air-containing spaces is often difficult. Metallic, non-ferromagnetic dental fillings, which cause serious artefacts on CT, do not affect NMR.

Adult↗

The use of bone grafts in the upper craniomaxillofacial skeleton.

While a plethora of new biologic and alloplastic materials are available for reconstruction of defects in the upper craniomaxillofacial skeleton, the role of free non-vascularized bone grafts remains an important one. In this review, this role will be defined in detail using case presentations to demonstrate a variety of ways in which such bone grafts may be helpful in reconstructing various facial skeletal defects.

Adult↗

Psammomatoid and trabecular juvenile ossifying fibroma of the craniofacial skeleton: two distinct clinicopathologic entities.

The term juvenile ossifying fibroma is used in the literature in naming 2 microscopically distinct fibro-osseous lesions of the craniofacial skeleton. One is characterized by small uniform spherical ossicles resembling psammoma bodies (psammomatoid juvenile ossifying fibroma). The other is distinguished by trabeculae of fibrillary osteoid and woven bone (trabecular juvenile ossifying fibroma). Three new cases of each type are reported, and the literature is extensively reviewed for published cases of these 2 entities.Psammomatoid juvenile ossifying fibroma is reported more commonly than trabecular juvenile ossifying fibroma. It affects patients from a wider age range (3 months to 72 years) and an older mean age range (16-33 years) as compared with 2 to 12 years and 8(1/2) to 12 years, respectively, for trabecular juvenile ossifying fibroma. In both types there is a slight male predominance and the lesions are unencapsulated and tend to infiltrate adjacent bone. A significant difference between the 2 tumors is their site of occurrence. Although psammomatoid juvenile ossifying fibroma occurs predominantly in the sinonasal and orbital bones, trabecular juvenile ossifying fibroma predominantly affects the jaws. Aggressive growth occurs in some-but not all-cases of both types. Such behavior may be related to younger patient age and the concurrent development of aneurysmal bone cysts, which is seen more frequently in psammomatoid juvenile ossifying fibroma. This study demonstrates that not only histologic but also demographic and clinical differences between psammomatoid juvenile ossifying fibroma and trabecular juvenile ossifying fibroma warrant their classification as 2 distinct clinicopathologic entities.

Adolescent↗

The collagen skeleton of the human umbilical cord at term. A scanning electron microscopy study after 2N-NaOH maceration.

The organization of the collagen fibrils in the human umbilical cord at term is directly visualized by means of a scanning electron microscopy cell maceration method. This technique clearly reveals that there is a much more extensive collagen fibrillar architecture within the umbilical cord than that reported in the classical histological descriptions. The Wharton's jelly, in fact, appears as a spongy network of interlacing collagen fibres and small woven bundles apparently arranged at random and forming a continuous soft skeleton that encases the umbilical vessels. The collagen fibrillar network shows the presence of a wide system of interconnected cavities consisting of canalicular-like structures as well as cavernous and perivascular spaces. This system of cavities might play a mechanical role allowing the storing of the ground substance of the jelly and its diffusion during twisting or compression. Furthermore, it may have an important role facilitating the diffusion throughout the jelly of diffused materials (i.e. water and trophic metabolites) either from or to the umbilical vessels and the amniotic cavity, thus overcoming the lace of a proper vasculature with the jelly.

Collagen↗

New Paleocene skeletons and the relationship of plesiadapiforms to crown-clade primates.

Plesiadapiforms are central to studies of the origin and evolution of primates and other euarchontan mammals (tree shrews and flying lemurs). We report results from a comprehensive cladistic analysis using cranial, postcranial, and dental evidence including data from recently discovered Paleocene plesiadapiform skeletons (Ignacius clarkforkensis sp. nov.; Dryomomys szalayi, gen. et sp. nov.), and the most plesiomorphic extant tree shrew, Ptilocercus lowii. Our results, based on the fossil record, unambiguously place plesiadapiforms with Euprimates and indicate that the divergence of Primates (sensu lato) from other euarchontans likely occurred before or just after the Cretaceous/Tertiary boundary (65 Mya), notably later than logistical model and molecular estimates. Anatomical features associated with specialized pedal grasping (including a nail on the hallux) and a petrosal bulla likely evolved in the common ancestor of Plesiadapoidea and Euprimates (Euprimateformes) by 62 Mya in either Asia or North America. Our results are consistent with those from recent molecular analyses that group Dermoptera with Scandentia. We find no evidence to support the hypothesis that any plesiadapiforms were mitten-gliders or closely related to Dermoptera.

Animals↗

Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals.

Msx1 is a key factor for the development of tooth and craniofacial skeleton and has been proposed to play a pivotal role in terminal cell differentiation. In this paper, we demonstrated the presence of an endogenous Msx1 antisense RNA (Msx1-AS RNA) in mice, rats, and humans. In situ analysis revealed that this RNA is expressed only in differentiated dental and bone cells with an inverse correlation with Msx1 protein. These in vivo data and overexpression of Msx1 sense and AS RNA in an odontoblastic cell line (MO6-G3) showed that the balance between the levels of the two Msx1 RNAs is related to the expression of Msx1 protein. To analyze the impact of this balance in the Msx-Dlx homeoprotein pathway, we analyzed the effect of Msx1, Msx2, and Dlx5 overexpression on proteins involved in skeletal differentiation. We showed that the Msx1-AS RNA is involved in crosstalk between the Msx-Dlx pathways because its expression was abolished by Dlx5. Msx1 was shown to down-regulate a master gene of skeletal cells differentiation, Cbfa1. All these data strongly suggest that the ratio between Msx1 sense and antisense RNAs is a very important factor in the control of skeletal terminal differentiation. Finally, the initiation site for Msx1-AS RNA transcription was located by primer extension in both mouse and human in an identical region, including a consensus TATA box, suggesting an evolutionary conservation of the AS RNA-mediated regulation of Msx1 gene expression.

Amino Acid Sequence↗

Visualization of the protein associations in the erythrocyte membrane skeleton.

We have obtained clear images of the erythrocyte membrane skeleton from negatively stained preparations that originate directly from the intact cell but in which the spectrin meshwork is artificially spread to allow close inspection. Our procedure requires less than 2 min at 5 degrees C in phosphate buffers. We find 200-nm-long spectrin tetramers crosslinked by junctional complexes. Each junction contains a regular 37-nm rod, probably an actin oligomer of approximately 13 monomers. Densities appear at variable places in the meshwork but distinct globules occur with great frequency 78 nm from the spectrin tetramer's junctional insertion end, very close to the known binding site for ankyrin. Most frequently, five or six spectrin tetramers insert into each junction, producing a meshwork that displays remarkably regular long range order.

Actins↗

Osteoclasts can be induced in fish having an acellular bony skeleton.

Kelp bass (Paralabrax clathratus) and leopard sharks (Triakis semifasciata) are characterized by an acellular (anosteocytic) bony skeleton and a focally calcified cartilaginous endoskeleton, respectively. These skeletal forms are not considered to function as mineral reservoirs. Previous studies showed that implanted bone particles are resorbed in rats by large multinucleated cells with ultrastructural features (ruffled borders) characteristic of osteoclasts. We tested the ability of fish to resorb bone matrix and to adapt to reduced salinity conditions. Bone particles were implanted in sharks and bass maintained in seawater (34 ppt, 40.5 mg of calcium per dl) or in diluted seawater (26 ppt, 28.5 mg of calcium per dl). Sera and elicited tissues were harvested 4 weeks later. In sharks, bone particles were not resorbed, and multinucleated cells were not evident under either normal or hyposalinity conditions. Shark sera were isoosmolar with the seawater or diluted seawater, with serum chemistries of the hyposalinity group reflecting the 23% reduction in environmental minerals and electrolytes, compared to sharks in normal seawater. In marked contrast, bass adapted to diluted seawater resorbed bone particles and maintained normal serum chemistries. Electron microcopy showed that the bone particles were surrounded by large, foamy multinucleated cells, many with membrane specializations typical of osteoclasts from higher vertebrates, i.e., extensive clear zones apposed to intact bone matrix and active ruffled borders overlying areas of matrix undergoing dissolution. Although osteoclasts had not been described in these fish, this study shows that bass have stem cells that can be stimulated to differentiate into bone-resorbing osteoclasts.

Journal Article↗

On the structure of erythrocyte spectrin in partially expanded membrane skeletons.

Spectrin is generally believed to play an important role in the erythrocyte membrane's ability to deform elastically. We have studied the structure of negatively stained spectrin in partially expanded membrane skeletons to determine how its molecular structure confers elastic properties on the cell membrane. Fourier analysis of electron micrographs of spectrin reveals that the alpha and beta subunits are twisted about a common axis, forming a two-start helix with twofold rotational symmetry. We propose that elastic deformation of the cell is mediated by transient extension of the helix by mechanical forces.

Computer Simulation↗