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At least 1,171 records · Page 65Linked to original sources

Nutrient fluxes in splanchnic tissue of dairy cows: influence of grass quality.

A crossover design was used to investigate the effects of high (450 kg of N/ha) or low (150 kg of N/ha) N fertilization of ryegrass on fermentation and nutrient fluxes in splanchnic tissue of dairy cows fed those grasses. Grass that was fertilized with the high amount of N contained more N and less sugar than did grass that was fertilized with less N. In rumen fluid, the concentration of NH3 N was lower for ryegrass that was fertilized with the low amount of N. The NH3 release by portal-drained viscera and urea synthesis in the liver were higher for cows fed ryegrass that was fertilized with the high amount of N. The concentration of NH3 N in rumen fluid, NH3 N release in portal-drained viscera, urea synthesis in the liver, urea release from the liver, and urea concentrations in milk were highly correlated. The release of acetate and propionate in portal-drained viscera was similar for both grasses and was well correlated with the proportion of volatile fatty acids in rumen fluid. The proportion of butyrate in rumen fluid was closely correlated with the release of butyrate and beta-hydroxybutyrate in portal-drained viscera. Glucose synthesis in the liver indicated gluconeogenesis from amino acids, which corresponded well with urea synthesis in the liver. For the grass fertilized with more N, availability of energy sources for rumen microbes was low, and, therefore, cows did not use the N in that grass efficiently.

3-Hydroxybutyric Acid↗

Crude protein and rumen undergradable protein effects on reproduction and lactation performance of Holstein cows.

We conducted a study to determine the effects of excess dietary crude protein (CP) and rumen undegradable protein (RUP) on reproduction and lactation performance of Holstein cows. During each of three yearly replicates, cows were blocked by previous mature equivalent milk production and randomly assigned at calving (n = 47; partum group) or at 42 +/- 21 d postpartum (n = 134; postpartum group) to the following dietary treatments: 1) ryegrass pasture supplemented with a corn and soybean meal grain mix (high CP, moderate RUP); 2) ryegrass pasture mornings and corn silage evenings, supplemented with grain as in diet 1 (moderate CP, moderate RUP control diet), and 3) ryegrass pasture mornings and corn silage evenings, supplemented with a grain mix containing corn, soybean meal, corn gluten meal, and blood meal (moderate CP, high RUP). Dietary CP and RUP concentrations were approximately 23.1, 5.8; 17.7, 5.0; and 17.2, 6.8% of dry matter for diets 1 to 3, respectively. Plasma urea N concentrations were highest in cows fed diet 1 (25.0 mg/dl), intermediate in cows on diet 2 (20.1 mg/dl), and lowest in cows on diet 3 (18.5 mg/dl). Cows fed excess dietary protein (diet 1) exhibited lower first breeding pregnancy rates (24.1 vs. 41.0%) and lower overall pregnancy rates (53.4 vs. 75.4%) than did cows fed diet 2, increasing time nonpregnant by an average of 15.1 d per cow. Reproductive performance was similar between cows fed diets 2 and 3. Mean fat-corrected milk (FCM) yield was not affected by protein concentration (diet 1 vs. 2); however, partum group cows that received supplemental RUP (diet 3) produced more 3.5% FCM than controls in early lactation. Feeding grain diets that contained excess dietary protein impaired the reproductive performance of dairy cows grazing ryegrass.

Ammonia↗

Absorption, excretion and tissue distribution of natural organic and inorganic zinc-65 in the rat.

The absorption and tissue distribution of organic and inorganic forms of 65-Zn were studied in the rat following single tracer oral doses. Rats dosed with 65-Zn grown into young corn and rye plants (organic form) absorbed and retained more 65-Zn in tissues than those given 65-Zn mixed with tissue paper (inorganic forms). A part of the beneficial effect was evident when 65-Zn was mixed with forage. The relatively high absorption values indicate that both forms were readily available, and thus incorporating zinc into young forage protein is not of major practical importance.

Animal Feed↗

The effect of grain component of the diet on the response of turkey poults to vitamin D3 and penicillin.

Three experiments were conducted with turkey poults to study the effects of the grain component of the diet (corn or rye), added fat, penicillin or extra vitamin D on growth and bone ash. In the first trial, where rye replaced corn in the diet, growth was depressed and the pults were rachitic before two weeks of age. Adding fat or penicillin to the rye containing diets significantly improved growth and increased bone ash. In the second trial, adding extra vitamin D3 or penicillin to a diet with rye increased both growth and bone ash. Diets containing either corn or rye were used in the third trial and the results again showed that extra vitamin D or penicillin markedly improved growth and bone ash when added to the diets with rye but did not significantly increase growth of poults on the diets with corn. Bone ash of turkeys fed the corn diets was significantly increased by a combination of extra vitamin D3 and penicillin.

Animal Feed↗

Variable rachitogenic effects of grain and alleviation by extraction or supplementation with vitamin D, fat and antibiotics.

Three experiments were conducted using day-old broiler type chicks to determine the effect of different cereal grains on vitamin D3 utilization and to investigate the effects of rye, corn, wheat and triticale as the cereal grain component of the diets on the development of a rachitic condition in chicks. Rye was submitted to acid treatment and water extraction in an attempt to destroy or isolate the rachitogenic factor. Results showed that with chicks fed a diet containing 200 I.U. of vitamin D3 with corn as the grain, mineralization of their bones was normal. Growth and bone ash were depressed when rye replaced corn in the diet. These effects were partially reversed when either fat or procaine penicillin was added to the diet, and comppletely prevented with a high level of vitamin D3 (2,000 I.U./kg.). In another experiment, triticale depressed bone ash even though it did not affect body growth to the same extent as rye. Our results indicate that the factor responsible for the rachitogenic condition of rye-fed chicks can be removed by water extraction of this grain or partially destroyed by acid autoclaved treatment. A combination of acid autoclaved treatment and penicillin supplementation prevented the depression in bone ash.

Animal Feed↗

Effect of ultraviolet light and oral vitamin D3 on rachitic chicks fed diets containing either corn or rye.

Three experiments using day-old chicks were conducted in battery brooders to further study the rye-vitamin D antagonism. Birds were fed a vitamin D3-free diet containing corn or rye and submitted to diverse treatments for the first 10 days. At this time the chicks were either continued on the same diet or changed to other grain-type diets. The effect of ultraviolet light exposure on the chicks and of a single oral dose of vitamin D3 was studied, and body weight gain and bone ash were determined after a one-week experimental period. Rachitic chicks on a corn diet responded significantly better than rye-fed chicks to a single oral dose of vitamin D3, based on bone ash of fat-free, dry tibia. This rachitogenic effect of rye was completely overcome by exposing the chicks to ultraviolet light or by water extraction or acid-autoclaving this grain. The results also demonstrate that the interference by rye does not persist after this grain is removed from the diet.

Administration, Oral↗

Effect of gamma irradiation, fractionation, and penicillin supplementation on the rachitogenic activity of rye for chicks.

Experiments were conducted to study the effect of gamma irradiation on the rye-vitamin D antagonism in broiler chicks. In an initial study, the irradiated grain was exposed to gamma rays for 6 hr (2 Mrad) and was fed to rachitic chicks for only 12 hr before a single oral dose of vitamin D3. In another trial, birds were fed during the entire experiment (12 days) on the different experimental diets, and the irradiated grain was exposed to gamma rays for 20 hr. Results indicate that the factor in rye that interferes with vitamin D utilization was largely inactivated by exposing this grain to gamma rays for 20 hr. Additional information is reported in this paper related to the alleviation by extraction or antibiotic supplementation on the rachitogenic properties of rye. These studies were conducted by feeding to rachitic chicks the test materials for only a short period of time (12 hr) before a vitamin D3 oral dose and measuring the bone mineralization 48 hr later. Results indicate that the rachitogenic factor in rye is not present in the ash portion of the grain, that it can be largely overcome by water extraction and penicillin supplementation, and that an organic solvent extraction has no effect. Cooked beans which depress growth and increase the growth response to antibiotics are not rachitogenic.

Animal Feed↗

The utilization of rye by growing chicks as influenced by calcium, vitamin D3, and fat type and level.

Four experiments were conducted with growing chicks to study the effects of feeding rye and wheat diets supplemented with different calcium levels, two types and two levels of fat, and increasing levels of vitamin D3 and to study the interrelationships of these dietary ingredients on chick performance. In Experiment 1, feed conversion efficiency was reduced to a much greater extend (10% vs. 1%) when tallow replaced soybean oil in a rye-based diet containing only .6% calcium compared to a wheat-based diet. When the dietary calcium was increased to 1.1% the replacement of tallow by soybean oil resulted in only a small reduction in feed efficiency for chicks fed either the rye-(3%) or wheat- (1%) based diets. The retention of fat, particularly tallow, was depressed in rye as compared to wheat-fed birds. Fat retention was also influenced by calcium levels. Increasing the dietary calcium from .6 to 1.1% improved tallow (16%) and depressed (7%) soybean oil retention in rye-based diets, whereas in wheat-based diets the retention for both fats was slightly decreased. Experiment 2 showed that the efficiency of utilization of rye diets containing 7.5% tallow was improved by 10% when the level of dietary calcium was increased from .6 to 1.1% while the corresponding improvement in similarly formulated wheat diets was only 1%. The improvement of 2.5% fat, however, was only about 3% for both wheat and rye diets. The results from Experiment 3 showed that in the presence of a large excess of vitamin D3, the depression in the efficiency of utilization of the wheat diet was 13% but was less than 1% for the rye diet. This indicates that rye contains a factor that depresses vitamin D3 absorption. In Experiment 4, when dietary calcium was increased from .80 to 2.00% or when penicillin (200 mg/kg) was added, the improvement in efficiency of feed utilization was greater in chicks fed a rye diet (7%) compared to those fed a wheat diet (1%). The rye diets, however, were always less efficiently utilized than the corresponding wheat diets. Vitamin D supplementation, in contrast, did not affect chick performance. These results show that the performance of chicks fed rye diets is markedly influenced by type and level of dietary fat and the level of dietary calcium.

Animal Nutritional Physiological Phenomena↗

Effect of gamma irradiation, penicillin, and/or pectic enzyme on chick growth depression and fecal stickiness caused by rye, citrus pectin, and guar gum.

The effects of gamma irradiation of rye, corn, pectin, and guar gum and supplementation of procaine penicillin and/or pectic enzyme (Irgazyme-100) were studied. Addition of 62.5% rye, 4% pectin, or 2% guar gum in place of corn significantly reduced chick growth (P < .05). Exposure to gamma irradiation, procaine penicillin, or pectic enzyme supplementation improved the growth of chicks fed rye. Growth response on the rye diet to one or more combinations of gamma irradiation, procaine penicillin, and pectic enzyme was greater than that of gamma irradiation, procaine penicillin, or pectic enzyme alone. Gamma irradiation or pectic enzyme supplementatin of pectin or guar gum improved chick growth and almost eliminated their growth depressing properties. A combination of gamma irradiation and pectic enzyme failed to give a further significant increase in growth over that of either alone. Addition of procaine penicillin to diets containing rye, irradiated pectin, or guar gum elicited a significant growth response, but the response was greatly reduced when diets contained irradiated guar gum or irradiated pectin. Feces of chicks fed diets containing rye, pectin, or guar gum adhered to screen floors in much greater amounts than for corn-fed chicks. Gamma irradiation, procaine penicillin, or pectic enzyme had no significant effect on fecal condition of birds fed diets containing rye or guar gum. In contrast, fecal condition of birds fed guar gum was significantly improved by a combination of gamma irradiation and pectic enzyme supplement. Gamma irradiation almost eliminated and pectic enzyme supplementation completely eliminated properties of pectin causing sticky feces. Results indicate that the component of rye that causes sticky feces was not changed by gamma irradiation and/or pectic enzyme, and, therefore, is different from the growth depressing factor and from citrus pectin.

Animals↗

Growth depression of chicks fed a crude rye extract containing pectic substances.

A crude extract with a high proportion of pectic substances was prepared from rye. This was fed to chicks in two studies to determine if rye pectic substances are responsible for the growth depression of chicks fed rye. The growth of chicks was significantly (P greater than .01) depressed when fed a diet containing this extract. Addition of penicillin to the diet improved the growth of chicks. Increasing the concentration of the extract in the diet produced an incremental depression in weight gain. The intestines of chicks fed this extract contained large quantities of gas that were reduced by penicillin supplementation. These results suggest that rye pectic substances may be responsible for the growth depression of chicks fed rye.

Animal Feed↗

The effects of a pectic enzyme on the growth-depressing and rachitogenic properties of rye for chicks.

Rye has growth-depressing and rachitogenic properties when fed to chicks. Growth depression also is observed when chicks are fed certain polysaccharides, particularly pectin. Rye has about 8% pectin-like material. A commercially available pectic enzyme preparation was fed to chicks at .1% of the rye diet. This enzyme permitted better utilization of rye for growth and also alleviated the rachitogenic effects of rye. The supplemental enzyme permitted maximal bone mineralization with 200 to 300 ICU of vitamin D3/kg diet, whereas 500 ICU/kg otherwise were required with rye diets.

Animals↗

The rachitogenic effects of fractions of rye and certain polysaccharides.

When fed to chicks rye is rachitogenic as well as growth depressing. The component or components of rye that cause these effects have not been identified. In an attempt to separate the factors, a water extract of rye was fractionated by precipitation with ethanol or ammonium sulfate. The precipitated fractions were fed to chicks. Although there were different responses to growth and bone ash from the ethanol fractions, they were not statistically significant. In another experiment, guar gum, pectin, or gum arabic was fed to chicks as 2% of the diet. Guar gum was both growth depressing and rachitogenic, pectin was only growth depressing, and gum arabic was without effect.

Animals↗

Influence of rye and dietary salt level on water and sodium metabolism in intact and colostomized roosters.

A study of water and Na metabolism was conducted with 24 roosters fed diets based on either corn or rye with two levels (.4 and 1.0%) of added salt. A diet containing water-extracted rye and a diet based on corn with a freeze-dried water extract of rye added at a level of 12% were also used in the study. Measurements were made on intact and colostomized roosters. In intact roosters dry matter metabolizability was depressed when rye diets were compared with corn diets. Measurements in feces from colostromized roosters fed rye as compared to those fed corn indicated that moisture, viscosity, and water holding capacity were increased and that bulk density was decreased. Diet salt level did not influence the responses regarding these parameters, but water extraction of rye completely alleviated the effects of rye on dry matter metabolizability and viscosity and partially reduced the influence of rye on fecal moisture while not altering the responses regarding water holding capacity or bulk density of feces. With the exception of feces moisture, the water extract of rye had no influence regarding these parameters. Roosters fed rye in comparison to those fed corn showed increases in water intake and excreta moisture content and decreases in urine sodium concentrations and plasma clearance of sodium. The response to rye regarding these latter parameters was dependent on diet salt level. The data indicated a reduced availability of sodium at the cellular level in rye-fed birds, which was manifest in a difference in route of excretion of sodium such that urine was the major route in corn-fed birds and feces was the major route for birds fed rye. The dietary fiber content of rye appeared to be a major factor in rye influencing sodium availability.

Animals↗

Effect of the addition of varied salt levels on the performance of growing chickens fed rye diets.

A series of feeding trials were conducted with Single Comb White Leghorn cockerels and broiler chickens to determine if salt per se was a contributing factor in the growth-depressing properties of rye. When added salt level increased from .3 to .6% in rye-based diets growth rate and feed conversion efficiency in young chickens improved significantly (P less than or equal to .05) but not to that of corn-fed birds. The relative growth rate of chickens fed rye diets containing .3% added salt was 78 to 86% of that of birds fed rye diets with .6% added salt.

Animals↗

The effect of saturation, chain length of pure triglycerides, and age of bird on the utilization of rye diets.

Four experiments were conducted with growing chicks to study the effects on performance and fat absorption of feeding rye and wheat diets supplemented with fats of different chain length and degree of saturation. In Experiment 1 it was shown that there was a much greater decrease in feed intake and fat absorption when tallow replaced safflower oil in the diets of chicks fed rye as compared to those fed wheat-based diets. Experiment 2 established the effects on chick performance of supplementing diets based on either rye or wheat with variably saturated fats. Substituting the most saturated triglyceride (C18:0) with the most unsaturated triglyceride (C18:2) in the rye diets improved the respective feed intake, weight gain, and feed conversion efficiency by 11, 75, and 40%, whereas the corresponding changes for the wheat-fed birds were -9, 9, and 16%, respectively. Experiment 3 investigated the effects on chick performance of supplementing rye-based or wheat-based diets with fats of varying chain lengths. Substituting fats containing long chain fatty acids (C18) for those containing shorter chain fatty acids (C12) resulted in respective improvements in weight gain and feed conversion efficiency of 27 and 30% in rye-fed birds and corresponding improvements of only 1 and 9% in the wheat-fed birds. A similar trend also occurred for fat absorption. The results of Experiment 4 demonstrated that older birds (35 versus 6 days of age) fed wheat-based diets absorbed only 1% more fat than younger birds, whereas when rye diets were fed, older birds absorbed 55% more fat than the younger birds. Overall it may be concluded that fat absorption in chicks is affected by chain length and degree of saturation of the fat and the age of the birds and that these factors affect rye-fed birds to a much greater degree than those birds fed wheat-based diets.

Age Factors↗