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At least 1,171 records · Page 65Linked to original sources
PubMed2004

Using STR analysis to detect human DNA from exploded pipe bomb devices.

This study investigated the possibility of recovering a bomb assembler's DNA from an exploded pipe bomb device. Metal and polyvinyl chloride (PVC) pipes were examined to determine if one surface type would allow better DNA recovery than the other. Ten subjects each handled components of one metal and one PVC pipe bomb. The bombs were exploded, the fragments were collected and swabbed using the double swab technique, and the samples were extracted, quantified, amplified, and genotyped using polymerase chain reaction/short tandem repeat (PCR/STR). Of the 20 bombs handled by the subjects, four bombs gave reportable results that matched the subject's known DNA profiles. An additional eight profiles, also matching the subject's known DNA profiles, were generated but were below the reportable threshold. There was no difference in the success rate of obtaining DNA profiles related to the use of either PVC or metal in the manufacture of the pipe bomb. The variables that appeared to have the greatest influence on the success of generating a DNA profile were the amount of fragmentation and subsequent recovery of the bomb fragments. It is suspected that successful DNA profiling could also be dependent upon the bomb assembler's propensity to slough skin cells on objects they handled.

Alleles↗
PubMed2004

Allele frequency distribution of D8S592 (STR) and PDGFA (VNTR) among five endogamous population groups of India.

Allele frequency distribution have been analyzed at D8S592 (short tandem repeat) and PDGFA (variable number of tandem repeat) among five distinct endogamous groups of India namely Ezhavas, Nayers, Arayas, Vishwakarma and Muslims. Muslims are religio-ethnic group while other populations mentioned above belong to distinct section of Hindu religion. All these populations are from Kollam district of Kerala in Southern India and speak Malayalam, an Indo-Dravidian language. A total of 228 for D8S592 and 212 for PDGFA loci, random, healthy individuals were analyzed.

DNA Fingerprinting↗
PubMedDate not supplied

[Rare, out-ladder alleles at the STR loci in the population of Upper Silesia].

Among individuals (participants of paternity testing) from the Upper Silesia region rare, out-ladder alleles in four STRs (routinely used in paternity testing) were found. There are alleles: VWA*12 and 21, CSF1PO*16, D13S317*6 and TPOX*5 with the following frequency: 0.0006 and 0.0006, 0.0005, 0.0006 and 0.0005.

DNA Fingerprinting↗