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PubMedDate not supplied

[Correction of schoolchildren's diets with biologically active additives].

The actual nutrition of schoolchildren in the Nizni-Novgorod district is imbalanced due to the deficiency of protein and vitamins and to the high contents of fats and carbohydrates. The authors provide evidence for a combined preparation to correct the children's diets, which incorporates animal protein, biologically active plant additives, and egg-shell calcium. The use of the preparation in combination with liquid bifidumbacterin contributes to increases in morphofunctional parameters in adolescents.

Adolescent↗
PubMed2002

Hypothesis for the influence of fixatives on the chromatin patterns of interphase nuclei, based on shrinkage and retraction of nuclear and perinuclear structures.

Nuclear chromatin patterns are used to distinguish normal and abnormal cells in histopathology and cytopathology. However, many chromatin pattern features are affected by aspects of tissue processing, especially fixation. Major effects of aldehyde and/or ethanol fixation on nuclei in the living state include shrinkage, chromatin aggregation and production of a 'chromatinic rim'. The mechanisms of these effects are poorly understood. In the past, possible mechanisms of fixation-induced morphological change have been considered only in terms of the theoretical model of the nucleus, which involves only a random tangle of partly unfolded chromosomes contained within the nuclear membrane. Such a model provides no basis for chromatin to be associated with the nuclear envelope, and hence no obvious clue to a mechanism for the formation of the 'chromatinic rim' in fixed nuclei. In recent years, two new models of nuclear structure have been described. The nuclear membrane-bound, chromosomal-domain model is based on the discoveries of chromatin-nuclear membrane attachments and of the localisation of the chromatin of each chromosome within discrete, exclusive parts of the nucleus (the 'domain' of each partly unfolded chromosome). The nuclear matrix/scaffold model is based on the discovery of relatively insoluble proteins in nuclei, which it suggests forms a 'matrix' and modulates gene expression by affecting transcription of DNA. Here, a hypothesis for fixation-associated chromatin pattern formation based mainly on the first model but partially relying on the second, is presented. The hypothesis offers explanations of the variations of appearance of nuclei according to fixation (especially air-drying versus wet-fixation with formaldehyde, glutaraldehyde or ethanol); the appearances of the nuclei of more metabolically active versus less metabolically active cells of the same type; the appearances of nuclei after fixation with osmium tetroxide; and of the marked central clearing ('egg-shell' or 'orphan Annie' appearance) of tumour nuclei of papillary carcinoma of the thyroid gland. A similar process may underlie the phenomenon of 'chromatin margination' seen in apoptosis. Various tests of the hypothesis, such as time-lapse confocal microscopy of living nuclei during fixation, are suggested. The significance of the theory is that it suggests that chromatin patterns could be investigated in terms of qualitative and quantitative aspects of nuclear components, and hence be related to the results of studies of the structure and function of nuclei in health and disease.

Cell Nucleus↗
PubMedDate not supplied

[Primary hyperparathyroidism. Round calcification as a sign of cervico-mediastinal localization of a parathyroid adenoma].

The authors report the case of a man aged 59 years, with primary hyperparathyroidism; removal of a first adenoma, did not lead to recovery. Later, an egg-shell calcification was found together with a second large cervico-mediastinal parathyroid adenoma, the removal of which caused all the symptoms to disappear. After a review of other cases in the world literature with calcification of the parathyroid glands, the authors consider, before using more complicated techniques for preoperative localisation of parathyroid adenomas, one should carry out careful radiological examination of the cervical and mediastinal region. Under these conditions, one may detect significant calcifications which have escaped routine examination and may contribute to the topographic diagnosis of the parathyroid lesion sought.

Adenoma↗
PubMedDate not supplied

[The conflict between optimisation and orderliness in building behaviour of the caddis fly, Chaetopteryx villosa Fabr.(Limnephilidae: Trichoptera), larvae].

The Optimal Foraging Theory predict that an animal should restrict its searching activity to those patches of environment, where the ratio of gain to time and energy spent is maximal. Experimenters usually verify the prediction against the null hypothesis of random activity distribution between patches. As animals always prefer profitable patches to some degree, experimental results can always be interpreted as confirmation of the theory. In opposite to this approach, we put forward the "regularity hypothesis". According to this hypothesis, the finding of a profitable item in some patch makes an animal to stay and test more items within the patch. The readiness to test decreases if the profitability of these items is low, and the animal eventually leaves the patch. It also searches in other patches less carefully. As a result, positive and negative responses to items are repeated by ordered series. In general, this regularity of responses helps animals to choose profitable patches. However, animals may also ignore some profitable patches because of the regularity, so that the behavioural optimazation fails. The regularity hypothesis have been tested in experiment with the search of building material and patch choice by caddisfly larvae. The first of two experimental patches contained egg shell fragments (profitable building material) mixed with a sand (unprofitable). The second patch contained sand only. Larva stayed within the first patch after a shell had been found, so that the probability to find more shells increased. However, larvae started walking after they had found several sand particles. Once starting to walk, they found new shell fragments and tested them, but tended to reject them and eventually leave the patch. Moreover, upon returning to the first patch, larvae also might reject fragments and leave the patch again. Rejections are accounted for by the fact that the duration of testing was too short to identify fragments correctly. As a result, negative responses were repeated to a certain degree independently of the profitability of building material, and the patch choice was not optimal. These results are agreed with the regularity hypothesis. It is argued that the hypothesis can be used as an alternative to the Optimal Foraging Theory.

Animals↗
PubMedDate not supplied

[A chewing gum with mineral additives].

Finely dispersed hen egg shell was added to a new chewing gum as a mineral component. The studies carried out in 28 schoolchildren have shown that 15 min chewing of this gum results in a marked elevation of salivary Ca levels and in improvement of the hygienic status of the oral cavity. This fact recommends this chewing gum as an additional means for care of the oral cavity for both hygienic purposes and for improving the mineralizing potential of the saliva.

Adolescent↗
PubMed1992

In vitro diffusion cell design and validation. II. Temperature, agitation and membrane effects on betamethasone 17-valerate permeation.

An in vitro permeation cell has been designed and validated for use in monitoring the transmembrane permeation of betamethasone 17-valerate. The design utilizes common laboratory equipment and incorporates as many beneficial features as possible from other designs. The importance of fully validating the hydrodynamic performance of the cell prior to experimentation is stressed. The cell was validated by monitoring the diffusion of betamethasone 17-valerate in isopropyl myristate solution into purified isopropyl myristate receptor phase at different temperatures, different agitation rates and through different synthetic and biological membranes. The results of the hydrodynamic validation agree with data from other researchers and show that the permeation cell is adequately sensitive to these experimental parameters. The results of the membrane evaluation allow appropriate selection of the barrier material for representative transdermal experiments to be conducted. While human and porcine stratum corneum/epidermis are similar in diffusive properties, hairless mouse skin appears to be the most convenient animal membrane for these studies. Although silicone and cellulose membranes appear to be useful in this application, porous filter membranes and egg-shell membranes are insufficiently discriminatory to betamethasone 17-valerate diffusion to provide useful in vitro permeation data.

Animals↗
PubMed2003

[An evaluation of the types and the results of surgical treatment for congenital scoliosis].

OBJECTIVES: We evaluated the types and the results of surgical treatment performed for congenital scoliosis. METHODS: Forty-one patients (26 females, 15 males; mean age 12.8 years) with congenital scoliosis were included. The patients were classified according to the Winter's system. Transpedicular hemiepiphysiodesis, "egg shell" procedure, and anterior hemiarthrodesis and posterior convex fusion were performed in infantile (n=1) and juvenile (n=8) patients. In the adolescent (n=32) group, posterior in situ fusion was performed for rigid curves (n=15), posterior fusion after correction with posterior instrumentation for moderate curves (n=8), and posterior fusion and correction with posterior instrumentation after an anterior osteotomy for segmentation failures (n=6). Five patients with unincarcerated fully segmented hemivertebrae had anterior or posterior instrumentation following anterior-posterior hemivertebrectomy. The mean follow-up was 51.8 months (range 26 to 132 months). RESULTS: Fourteen patients (34.2%) had formation failures, 19 patients (46.2%) had segmentation failures, and eight patients (19.6%) had mixed types of deformities. Klippel-Feil syndrome was detected in two patients, and heart valve abnormality was found in two patients. Although the infantile patient who underwent transpedicular hemiepiphysiodesis showed no improvement after surgery, a spontaneous correction rate of 60% was found during her final controls. Patients who underwent anterior hemiarthrodesis obtained a final correction rate of 54.3% following a 42% of correction at surgery. The correction rates for posterior instrumentation were 26.2% with translation and 49.3% after an anterior osteotomy. Patients who had anterior or posterior instrumentation after anterior-posterior hemivertebrectomy had final correction rates of 73.3% and 59.2%, respectively. Final evaluations showed that nine patients (21.9%) had no change in their curves, while 10 patients (24.4%) had spontaneous correction. Four patients developed superficial (n=2) and deep (n=2) infections following posterior instrumentation. Of these, three patients were successfully treated with debridement and antibiotic therapy, whereas one patient required implant removal in the eighth month. No neurologic deficits or systemic complications occurred during or after surgery. CONCLUSION: Surgical treatment may yield successful results in progressive congenital scoliosis when an appropriate surgical technique is selected based on the patient's age and the type of deformity.

Adolescent↗
PubMed2006

[A case of silicotuberculosis with difficulty in its diagnosis].

A 68-year-old man who had worked as a stone mason for more than 50 years with a heavy smoking history consulted our clinic with symptoms of cough, low grade fever, weightloss, malaise and a single expectoration of hemo-sputum. He had been diagnosed as silicosis by the mass survey 5 years ago based on nodular shadows with egg-shell calcification in hilar lymphnodes on his chest radiography, and has received chest radiographic examination once a year. As the author was not so familiar with the radiographic features of silicotuberculosis, it was difficult to interprete ill-defined contour of silicotic nodules accompanied by patchy opacities formation in right midlung field and silicotic conglomeration accompanied by an ischemic cavity in the left basal segments. A definitive diagnosis could not be established until 10 months later when a second attack of exacerbation of silicotuberculosis occurred showing multiple thin walled fresh tuberculous cavities on the chest radiography with positive smear and culture. Among multiple tuberculous cavities, there was a cirrhotic-walled cavity caused by endogenous reactivation of a quiescent tuberculous lesion on the right apex. This lesion was considered to be the source of dissemination of this case. Finally, it took about two and a half years before establishing the diagnosis in this case because of a series of doctors delays. He was treated successfully with antituberculous drugs for one and a half years including one year rifampicin medication. The clinico-pathological findings of silicotic conglomeration in the left basal segments were discussed based on the findings of transbronchial biopsy from occluded B10 and chest radiographic findings, and it was revealed that silicotic conglomeration might consist of inflammatory granulation combined with granulomatous tubercle, but not a fibrous lesion.

Aged↗
PubMed2006

Analysis of odontogenic cysts of the jaws.

In this study 850 cases of odontogenic cysts of the jaws, treated in Kaunas University of Medicine Hospital during the period of 1986-2004, were analyzed in order to evaluate the incidence of these cysts and other aspects of clinical and therapeutic interest such as their clinical features, changes they caused in the facial and mandible structures, and the teeth that had caused the pathology. Case histories of 455 men and 395 women had been analyzed. The age of the patients varied from 4 to 87 years, with the average of 35.8. More than half of the diagnosed cysts (63%) were present in the maxilla, 37% of them--in the mandible. The common complaints of the patients were bump, swelling, pain, discharge leaking from the fistula or alveolus after tooth extraction, increased teeth mobility, paresthesia; changes in the maxillofacial system - intraoral or facial asymmetry, inflated bone with sensation of an egg shell cracking revealed under palpation, swelling at the mucofacial fold, mobile teeth and fistula. While comparing the causative groups of antagonistic teeth in the maxilla and mandible, we found the proportion to be (in order from the right to the left): molars--1:1, premolars 1.3:1, fore teeth--3.3:1, fore teeth 3.8:1, premolars 1.2:1, molars 0.7:1. Odontogenic cysts may be present in both sexes; maxillary cysts are 1.5 times as frequent as mandibular cysts. The pathology may equally affect both sides of jaws, most cysts being diagnosed in the maxillary fore teeth area from tooth 13 to tooth 23. The following pairs of changes in maxillofacial system are frequently present: inflated body of bone or alveolus--increased mobility of teeth; the formed fistula--swelling at the mucofacial fold, swelling at the mucofacial fold--pain. The applied treatment of cysts was surgical--cystotomy or cystectomy.

Adolescent↗