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Results for “Tooth Calcification”

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At least 1,153 records · Page 64Linked to original sources
PubMed1978

[Ultrastructural study of odontogenesis in the frog (Rana temporaria L.)].

Differentiation of the cells of the dental bud which produce the hard tissues of teeth was studied at the ultrastructural level; the activity of these cells (odontoblasts and ameloblasts), the laying down of organic matrices elaborated by them, the mineralization of these matrices, and the maturation of dentine and enamel are described. Injections of tritiated proline enabled us by the autoradiographic method, to confirm and to indicate more precisely the activity of these cells. Dental calcified tissues of Rana exhibit the general properties of these tissues among Vertebrates: high content of apatite, low content of organic materials (collagenous in dentine, non-collagenous in enamel), precise orientation of crystals, and true but non-prismatic enamel. The enamel of Anura as well as enamel of Urodela is a true enamel of epithelial origin.

Ameloblasts↗
PubMed1986

Development of mouse embryonic molars in vitro: an attempt to design defined culture conditions allowing mineralization.

Mandibular first molars from 17 day old mouse embryos were cultivated in vitro for 14 days in BGJb-medium supplemented with 20% horse serum and 10% chick embryo extract or in BGJb containing various compounds suggested to have a role in biological mineralization. The incubation atmosphere consisted either of 5% CO2 in air or 50% O2, 45% N2 and 5% CO2. Histologically, explants grown in serum-supplemented medium frequently showed dentin mineralization, the frequency being highest, 90%, in high oxygen partial pressure. In medium without serum, only two out of 43 explants which were cultured in BGJb containing 5 mM Na-beta-glycerophosphate, formed mineralized dentin. The amounts of DNA and calcium, and the activity of alkaline phosphatase in explants after culture showed no direct correlation to mineralization potential. Calcified dentin was always confined to one or two clearly demarcated areas, which were intensely stained by the von Kossa method. It appears that mineralization continues readily when it has started and that it is the initiation of mineralization that is the critical threshold and depends on serum factors.

Alkaline Phosphatase↗
PubMed1986

Mantle dentine in man--a quantitative microradiographic study.

50 microradiographs taken in a standardized manner of midsagittal ground sections of teeth of individuals aged 18 to 56 years were densitometrically evaluated along a track passing through enamel, dentine and an aluminium stepwedge. Semi-quantitative analysis of mineral density uniformly showed an irregular platform representing circumpulpal dentine and a peripheral down slope in the region of the amelodentinal junction, representing mantle dentine. The width of this less mineralized peripheral zone measured on densitometric recordings averaged 150 microns (+/- 50). Quantitative analysis of the two dentinal regions permitted the calculation of the mineral content in terms of volume percentage using both a graphic method and an electronic computer method. The sections were also examined by polarized light microscopy which clearly visualized the presence of peripheral mantle dentine. The mean mineral density of circumpulpal dentine was 46% according to both the graphic and the computer methods; mantle dentine yielded means close to 42% according by both methods. The 4% difference in density between circumpulpal dentine and mantle dentine proved to be statistically significant; there was no significant difference between the means obtained graphically and those obtained electronically. The need for further investigation of this region of the amelodentinal junction was stressed.

Adolescent↗
PubMed1978

The mechanical bond.

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Acid Etching, Dental↗
PubMed1986

Biochemical characterization of alkaline phosphatase from partly mineralized bovine enamel.

Alkaline phosphatase (AP) from partly mineralized bovine enamel was studied. The enzyme resembles alkaline phosphatases in other calcifying tissues. The Km was 0.46 mM; the phosphatase was strongly inhibited by 1 mM Levamisol or EDTA and it showed the same sensitivity towards heating as bone alkaline phosphatase. The inactivation caused by phosphate ions was approximately about 30%. In a zymogram, five isozymes of enamel alkaline phosphatase can be recognized. In a SDS-polyacrylamide gel a single band showing enzyme activity was visualized. This enzyme has an apparent molecular weight of 140,000.

Alkaline Phosphatase↗
PubMed1987

Effects of acid-base status and fluoride on the composition of the mineral in developing enamel and dentine in the dog.

Developing molar teeth of the dog were sectioned, embedded in copper containing polymethyl methacrylate, polished and their Ca/P and Ca/Na molar ratios investigated with the electron microprobe. The teeth were obtained at 30 days from 9 pups fed regimes of different acid-base status with or without fluoride supplementation from birth to sacrifice at 30 days. No clear trends in their Ca/P or Ca/Na ratios with variation in the diet were observed. However, evaluation of the Ca/P ratio of the enamel as a function of depth revealed that this ratio was 0.80 +/- 0.15 at the mineralization front. This suggests that in enamel brushite rather than octacalcium phosphate is the precursor phase of the mineral.

Acidosis↗