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Using the spectral analysis of the EEG for prognosis of severe brain injuries in the first post-traumatic week.

Fifty adult patients with severe brain injuries were examined with the EEG on the 1st, 2nd, 3rd, 5th, and 7th postoperative days. In 40 of these 50 cases a prognosis was given after 7 days as a result of the Fourier analysis of the EEG: 23 patients survived, 17 died. There are significant differences in the EEG's for these two groups: an increase of the absolute and relative amplitudes in the alpha and theta bands for the survivors, and a decrease or no change in the alpha and theta bands for the others, all during the first week. In 10 cases the correct prognosis could not be given: 5 patients showed spindle or alpha activity (alpha pattern coma) in the EEG; 4 cases had to be treated with barbiturates for focal seizures, and one suffered from secondary bleeding. In these cases computer tomography did not yield additional prognostic information.

Adult↗

Spectral analysis of electromyogram in lumbar muscles: fatigue induced endurance contraction.

In the last few years, epidemiological studies have shown correlations between the low endurance capacity of the erector spinae muscle and low back pain (LBP). In this study, the function of the erector spinae muscle and localized muscle fatigue in LBP were investigated in both LBP patients and control subjects without LBP, using surface multi-channel electromyograms (EMG). Four positions (L1, L2, L4, L5) were chosen as representative locations for the different functions of the erector spinae muscle. Median frequency (fm) parameters of an EMG power density spectrum were monitored to quantify localized muscle fatigue. This research indicated a statistically significant difference (P < 0.05) in fm parameters between the LBP patients and the control subjects. The integrated electromyogram (iEMG) signals of LBP patients were considerably higher than for the control subjects during endurance isometric contraction. Our research suggested that human fatiguing erector spinae muscle, in submaximal voluntary contractions tests, induced a different firing order of motor unit spikes between two groups.

Adult↗

Dose-response relationship of vindeburnol based on spectral analysis of posturographic recordings.

The dose-response relationship of vindeburnol has been investigated by assessing postural activity in a population of elderly patients, using posturography, an objective method for measuring balance. A controlled double blind trial was done in two periods: during the first week each patient received placebo, and during the second week either placebo or vindeburnol 30, 60 or 90 mg/d was given. Subjects underwent three posturographic recordings at weekly intervals (prior to treatment, after one week on placebo and after one week of treatment). There was no placebo effect. A significant decrease in the sagittal and lateral energies of body sway was found after vindeburnol, which indicates an improvement in balance. The improvement was proportional to the daily dose of vindeburnol.

Aged↗

VO2 kinetics in subjects differing in aerobic capacity: investigation by spectral analysis.

The power-VO2 transfer functions of 38 subjects differing in aerobic capacity have been determined on the basis of breath-by-breath total oxygen uptake (VO2,t) measurements during light cycle ergometer exercise (lactic acid concentrations below 2 mmol.l-1). At constant pedalling frequency (1 Hz) pseudorandom binary sequences (PRBS) of workload were used as the testing signal. The VO2,t response was analysed by autocorrelating the ergometer power input and crosscorrelating the power input and VO2,t output. From the spectra of these functions the amplitude ratios and phase relationships were computed for the first six harmonics of the PRBS fundamental (14 mrad X s-1). We found that differences in aerobic capacity are associated with significant differences in the amplitude plots of the VO2 transfer function.

Adult↗

Relationship between age and heart rate variability in supine and standing postures: a study of spectral analysis of heart rate.

We compared heart rate variability measures of 11 normal children (4-12 years) to 23 normal adults (21-43 years) to study the effect of age on heart rate variability measures. Children had a significantly higher supine and standing heart rate and lower supine and standing systolic and diastolic blood pressure. Children also had a significantly higher supine standard deviation of HR, supine low frequency (0.01-0.05 Hz) (p < 0.005), supine high frequency (0.2-0.5 Hz) (p < 0.001), and standing high frequency powers (p < 0.005) compared to adults. The ratio of mid frequency (0.07-0.15 Hz) to high frequency power (0.2-0.5 Hz) upon standing was significantly lower in children (p < 0.005). While there was a significant increase of the mid frequency power from supine to standing posture in the adult group (p < 0.02), there was no such significant increase in children. There were also significant negative correlations between age and supine low frequency, mid frequency, and high frequency powers, and standing high frequency power. These findings illustrate a decrease of cholinergic and an increase of adrenergic modulation of heart rate variability with age (4-43 years).

Adrenergic Fibers↗

Spectral analysis of a series of partially protected and deprotected tetrapeptides, analogues of AS-I phytotoxin.

A series of six tetrapeptides, analogues of AS-I phytotoxin, pathogenic to sunflower, have been synthesized either in solution and/or by solid phase methods and have been tested for phytotoxic activity in various plants and cytotoxic activity in three cancer cell lines. These peptides were identified as model compounds by fast atom bombardment (FAB), plasma desorption (PD), electrospray ionization (ESI) mass spectrometry and by 1H, 1H-1H, 13C and 1H-13C NMR. The data presented show that in protected tetrapeptides the molecular ion was easily identified whereas some difficulties appeared with the fully deprotected peptides. NMR spectra are given.

Magnetic Resonance Spectroscopy↗

Effects of baroceptor and cardiopulmonary reflexes on neural control of heart rate in normal man, as assessed by power spectral analysis.

This study was designed to establish the neurally-mediated effects of baroceptor and cardiopulmonary reflexes on heart rate in normal subjects. We therefore studied the effects of various interventions, able to modify the activity of arterial baroreceptors and cardiopulmonary receptors, on the power spectrum of the R-R interval in nine healthy men. To confirm the efficacy of these interventions left ventricular volume was monitored using a portable radionuclide probe. Isosorbide dinitrate (5 mgm sublingually) while sitting unloads both baroreceptors and the cardiopulmonary receptors; it decreased left ventricular end-diastolic volume (p < 0.01) and the mean R-R interval (p < 0.005), increased the power of the low-frequency (LF) component of the R-R interval (p < 0.01), decreased the power of the high-frequency (HF) component (p < 0.005) and increased the LF/HF ratio (p < 0.05). The application of cuffs around the thighs in the supine position, which unloads only cardiopulmonary receptors, decreased left ventricular end-diastolic volume (p < 0.05) but did not affect mean R-R interval, LF component, HF component and LF/HF ratio (p > or = 0.10). Leg raising, which loads only cardiopulmonary receptors, increased left ventricular end-diastolic volume (p < 0.05) and did not affect the mean R-R interval, LF component, HF component and LF/HF ratio (p > or = 0.10). In conclusion, our data suggest that, in normal man, baroreceptor unloading increased sympathetic and decreased parasympathetic neural control of heart rate; cardiopulmonary reflexes do not appear to have a direct neurally mediated effect on heart rate.

Adult↗

Super-high sensitivity systems for detection and spectral analysis of ultraweak photon emission from biological cells and tissues.

In this paper we summarize and discuss the modern technology and systems, studied and established by our research group, for performing the detection and special analysis incorporated with the super-high sensitivity photon counting method for the study of ultraweak photon emission; for example, extra-weak bioluminescence and chemiluminescence from living cells and tissues, closely related to biochemical and biophysical processes and activities. An excellent sensitivity of the basic photon counting system, making it possible to achieve count rates in the very low range of one photoelectron per second to one per minute, allowed us to carry out in vivo as well as in vitro measurements, and analyses of ultraweak bioluminescence and chemiluminescence. Recent results concerning ultraweak photon emission from blood samples and organ homogenates of rats are presented and reviewed as one of the interesting and valuable applications of our modern technology for studying ultraweak cell and tissue radiation.

Animals↗