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An overview of exposure to, and effects of, petroleum oil and organochlorine pollution in grey seals (Halichoerus grypus).

Most incidences involving oil pollution of grey seals (Halichoerus grypus) seem to have occurred at the breeding sites. Because of the high concentration of animals at this time, even small oil spills will pollute many animals. As a result of chronic low-level pollution from coastal ship traffic and discharges from offshore petroleum activity in the North Sea, approximately 50% of the grey seal pups at the largest breeding colony in Norway are polluted each year by oil. In this case, as well as in other similar cases of spills at breeding colonies, oil has produced little visible disturbance to the seals behaviour and there has been little mortality. The effects and mortality may, however, be more serious following a spill of crude oil, where animals may be affected by inhalation of toxic volatile compounds. High body burdens of PCBs and DDTs seem to have caused skull-bone lesions and occlusions of the uteri in grey seals in the Baltic Sea. Exposure to these persistent compounds has also been suspected to be the cause of reduction in the population of Baltic grey seals. There are indications that thyroid hormone and vitamin A status of grey seal pups are affected by the low exposure concentrations experienced at the Norwegian coast (approximately 1/20 of the concentration detected in grey seal pups from the Baltic Sea). This gives serious cause for concern about the effects that chronic low-level exposure to persistent organochlorine pollutants may have on individuals and on populations of grey seals.

Animals↗

Ultrastructure in leaves of Fagus silvatica and Carpinus betulus individuals tolerant and susceptible to SO2 and heavy metal pollutants.

The ultrastructure characteristics of leaves during the development of cell organelles, in the "greening process" of spring, have been studied in pollutant-tolerant and susceptible individuals of beech (Fagus silvatica) and hornbeam (Carpinus betulus) in an area polluted with SO2 (the main pollutant may reach 0.06-2.72 mg/m3), heavy metals, and other gases discharged by non-iron metal processing factories. Leaves from tolerant beech and hornbeam individuals did not undergo ultrastructural changes compared to control leaves in an unpolluted area. The susceptible and very susceptible individuals of both species gradually undergo modification in organelle ultrastructure not only during the first steps of greening and momentary exposure to air pollution, but also as a result of long-term exposure to chronic pollution in the forest.

Air Pollutants↗

Air pollutants and respiratory hypersensitivity.

Epidemiological evidence suggests that an increase in liquid petroleum derived pollutants is associated with exacerbation of allergic airway disease, and that the effects of pollution may occur 1-2 days later. Laboratory based studies have demonstrated that the pollutants responsible for the adverse effects on respiratory health include nitrogen dioxide (NO2), sulphur dioxide (SO2), ozone (O3) and respirable particulates (PM10). More recently, studies of asthmatic individuals exposed to O3, NO2 and a combination of NO2 and SO2 have indicated that these agents increase the airway responsiveness of these individuals to inhaled allergen, and that this effect may be maximal 24 h after exposure to the pollutants. Studies investigating the putative mechanisms underlying the effects of these pollutants suggest that exposure to these agents may lead to perturbation of the airway epithelium and release of pro-inflammatory mediators from the epithelial cells, which then influence the activity of inflammatory cells, such as eosinophils.

Adult↗

Chromatographic separation of marine organic pollutants.

A review and discussion of the chromatographic separation of marine organic pollutants is given, including sampling and clean-up procedures, fractionation and enrichment of marine pollutants, capillary gas chromatography (cGC) and high-performance liquid chromatography applying both classical and chiral stationary phases. The potential of multi-dimensional cGC for the analysis of marine organic trace pollutants is discussed for polychlorinated biphenyls (PCBs). The chromatographic separation of coplanar PCBs and of the enantiomers of chiral pollutants provides a further insight into the toxic potential of these marine organic pollutants.

Chromatography↗

Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): differential expression in fish from polluted and unpolluted environments.

Populations of killifish (Fundulus heteroclitus) persist in many different highly polluted environment indicative of adaptation or tolerance. In this study, we sought to determine whether long term, multigenerational exposures to environmental contaminants has affected reproductively relevant genes and biological processes. A homology cloning strategy was used to isolate the killifish cytochrome P450 aromatase (P450arom, estrogen synthetase) cDNAs. Consistent with previous fish studies, killifish were found to have two P450arom forms, which segregated into A- and B-gene clades and were differentially expressed in brain (B >> A) and ovary (A >> B). Comparison of killifish from highly polluted (New Bedford Harbor, NBH) and unpolluted (Scorton Creek, SC) environments revealed no site-related differences in P450arom coding sequences or in overall tissue distribution patterns. As measured by real-time quantitative PCR (QPCR) analysis, however, P450arormB (a known marker of estrogen effect) was approximately two-fold higher in the brain of NBH than of SC fish, a difference seen in reproductively active and inactive males and females. Providing further evidence of exposure to estrogen-like pollutants or metabolites in NBH, vitellogenin (vtg) mRNA and protein were elevated in seasonally active and inactive males, and in reproductively inactive females, when compared to SC fish. By contrast, during the period of reproductive activity, NBH females had a lower gonadosomatic index, lower plasma estrogen, a decreased hepatosomatic index, and reduced vtg expression as compared to SC females, indicating that the female hypothalamic-pituitary-gonadal (HPG)-liver axis is impaired in the polluted environment. As measured by a decrease in plasma androgen (but not GSI), the male HPG axis was impaired in reproductively active NBH versus SC fish. In agreement with reports that NBH killifish are resistant to dioxin-like chemicals (DLC) that activate arylhydrocarbon receptor (AhR) signaling, ovarian P450aromA (a marker of dioxin-like effect in zebrafish embryos) did not differ in SC and NBH fish. In conclusion, the killifish population at the NBH Superfund site maintains a level of reproductive competence in the face of evidence of exposure to estrogen-like pollutants and endocrine disruption.

Amino Acid Sequence↗

Speciation as a screening tool for the determination of heavy metal surface water pollution in the Guadiamar river basin.

The Guadiamar river basin has traditionally received pollutants from two main sources: in its northern section of mining origin, and in its southern section (next to Doñana National Park) from urban-industrial and agricultural sources. In April 1998, the spill of 6 million m3 of mining wastes (acidic waters and sludge) severely polluted the Guadiamar river basin with heavy metals, which caused serious damage to the local ecosystem. There is a direct association between the physicochemical speciation of an element and its toxicity, biological activity, bioavailability, solubility, etc. This work describes a distribution study of the metals Zn, Cd, Pb and Cu by speciation analysis of surface waters in eleven sampling points of the Guadiamar river basin. Four metal fractions were determined using anodic stripping voltammetry: labile metal forms, H+ exchangeable metal forms, strongly inert forms (associated with organic and inorganic matter in solution), and forms associated with suspended matter. Total concentrations in surface waters followed the trend Zn > Cu > Pb > Cd. The speciation study showed that Zn and Cd were present to a large extent in available forms (labile and H+ exchangeable), while Pb and Cu were found mostly in the less available forms (strongly inert). Moreover, the available forms were found in the northern section (mining pollution) and the strongly inert forms in the southern section (urban, industrial and agricultural pollution). These results can illustrate the potential value of speciation to discern between different sources of pollution.

Ammonia↗

Assessment of heavy metal pollution in surface soils of urban parks in Beijing, China.

Assessing the concentration of potentially harmful heavy metals in the soil of urban parks is imperative in order to evaluate the potential risks to residents and tourists. To date, little research on soil pollution in China's urban parks has been conducted. To identify the concentrations and sources of heavy metals, and to assess the soil environmental quality, samples were collected from 30 urban parks located in the city of Beijing. Subsequently, the concentrations of Cu, Ni, Pb and Zn in the samples were analyzed. The investigation revealed that the accumulations of Cu and Pb were readily apparent in the soils. The integrated pollution index (IPI) of these four metals ranged from 0.97 to 9.21, with the highest IPI in the densely populated historic center district (HCD). Using multivariate statistic approaches (principal components analysis and hierarchical cluster analysis), two factors controlling the heavy metal variability were obtained, which accounted for nearly 80% of the total variance. Nickel and Zn levels were controlled by parent material in the soils, whereas Cu, Pb and, in part, Zn were accounted for mainly by anthropogenic activities. The findings presented here indicate that the location and the age of the park are important factors in determining the extent of heavy metal, particularly Cu and Pb, pollution. In addition, the accumulation of Zn did not appear to reach pollution levels, and no obvious pollution by Ni was observed in the soils of the parks in Beijing.

China↗

Persistent organic pollutants (POP) in a benthic omnivore--a comparison between lake and stream crayfish populations.

We investigated the accumulation of PCB and DDT in crayfish populations in 10 streams in southern Sweden. The results were compared with an earlier study on crayfish in lakes from the same area. We found that the concentration of pollutants in crayfish did not differ between the two types of systems. Variation in body burden was higher in stream living crayfish probably because of the higher influence from pollutants deposited in the catchment area and the more dynamic transport in streams. In streams, p,p'-DDE concentrations were positively correlated to trophic status (total phosphorous) while PCB did not show any correlation with the nutrient regime. Further, mean SigmaPCB and p,p'-DDE concentrations in crayfish did not correlate in streams. We suggest that the sources of the two pollutants differ for stream living crayfish. The results indicate that crayfish in streams are affected to a higher degree to pollutants in the catchment area and the precipitation regime. In lakes, internal processes govern uptake of pollutants in crayfish.

Animals↗

Toxicity and DNA damage in tobacco and potato plants growing on soil polluted with heavy metals.

Heterezygous tobacco (Nicotiana tabacum var. xanthi) and potato (Solanum tuberosum var. Korela) plants were cultivated on soil from the site Strimice which is highly polluted with heavy metals and on nonpolluted soil from the recreational site Jezerí, both in North Bohemia, Czech Republic. The total content, the content of bioavailable, easily mobile, and potentially mobile components of heavy metals (Cd, Cu, Pb, and Zn) in the tested soils, and the accumulation of these metals in the above-ground biomass and roots of tested plants were analyzed by flame atomic absorption spectrometry or flameless atomic absorption spectrometry. The average tobacco leaf area and potato plant height were significantly reduced in plants growing on the polluted soil. We have measured the DNA damage in nuclei of leaves of both plant species using the Comet assay. A small but significant increase in DNA damage was noted in plants growing on the polluted soil versus controls. As the tobacco and potato plants with increased DNA damage were severely injured (inhibited growth, distorted leaves), this increase may be associated with necrotic or apoptotic DNA fragmentation. No increase in the frequency of somatic mutation was detected in tobacco plants growing on the polluted soil. Thus, the polluted soil probably induced toxic but not genotoxic effects on tobacco and potato plants.

Comet Assay↗

A review of traffic-related air pollution exposure assessment studies in the developing world.

Exposure assessment studies in the developing world are important. Although recent years have seen an increasing number of traffic-related pollution exposure studies, exposure assessment data on this topic are still limited. Differences among measuring methods and a lack of strict quality control in carrying out exposure assessment make it difficult to generalize and compare findings between studies. In this article, exposure assessment studies carried out in the developing world on several traffic-related air pollutants are reviewed. These pollutants include particulate matter (PM), carbon monoxide (CO), nitrogen dioxide (NO(2)), volatile organic compounds (VOCs), and polycyclic aromatic hydrocarbons (PAHs). In addition, it discusses advantages and disadvantages of various monitoring methods (ambient fixed-site monitoring, microenvironment monitoring, and personal exposure assessment using portable samplers) for these pollutants in exposure assessment studies. Also included in this paper is a brief introduction of standards for these pollutants in ambient air or in occupational settings established by the United States Environmental Protection Agency (USEPA), the United States Occupational Safety and Health Administration (OSHA) and the World Health Organization (WHO). The review ends with a summary of the limitations and gaps in recent studies and suggestions for future research in the developing world.

Air Pollutants↗

Evaluation of the usefulness of bird feathers as a non-destructive biomonitoring tool for organic pollutants: a comparative and meta-analytical approach.

In this study, we investigated whether bird feathers can be used as a non-destructive biomonitor for organic pollutants. We analysed the outermost tail feathers of 8 terrestrial and aquatic bird species from Belgium (8 species, n=108) for polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and organochlorine pesticides (OCPs). Every compound class could be quantified in one single tail feather of the birds under study (sum PCBs ranging from 5.5 to 510 ng/g feather, sum PBDEs from 0.33 to 53 ng/g feather, sum DDTs from 1.5 to 730 ng/g feather), except for PBDEs in feathers of the common moorhen (Gallinula chloropus). Further, we calculated Pearson correlations between concentrations of organic pollutants in feathers and concentrations in corresponding muscle or liver tissue from the birds. Correlations were found significant in half of the cases of the terrestrial species, but were found not significant for the aquatic species, with the exception of a significant correlation of sum PCBs in the common moorhen. Only for the common buzzard (Buteo buteo) (n=43) all correlations were found significant (0.32<r<0.77). In order to cope for low statistical power, we performed a meta-analysis on all bird species together. This led to significant correlations between levels in feathers and corresponding levels in muscle or liver for all terrestrial birds (p<0.05 in all cases, effect size 0.59 (p,p'-DDE) to 0.71 (Sigma PCB) for levels in feather and muscle). When correlations were recalculated excluding the birds that had died due to starvation, correlation coefficients for the terrestrial birds were found even higher (effect size up to 0.83 (Sigma PCB)). These results have important implications for non-destructive and retrospective biomonitoring. Although our results suggest that exact concentrations in the body cannot be predicted using feathers, bird feathers can give a good estimate of contamination levels in a population and as such are a potential non-destructive biomonitoring tool for organic pollutants. Outermost tail feathers and muscle tissue were also examined for nitrogen (delta15N) and carbon (delta13C) stable isotope content in the different bird species. However, delta15N/delta13C signatures in feather or muscle were not significantly correlated with the corresponding levels of organic pollutants in these tissues. Various confounding factors (such as habitat, condition, age, sex) may have masked a relationship between delta15N values and organic pollutant levels in the birds under study.

Animals↗

The quantification and distribution of pollution Pb at a woodland in rural south central Ontario, Canada.

Lead concentrations and Pb isotope ratios were measured in the forest floor, mineral soil and vegetation at a white pine and a sugar maple stand in a woodland in south central Ontario. Lead concentrations decreased and 206Pb/207Pb ratios increased with mineral soil depth reflecting the mixing of pollution and natural Pb sources. Lead concentrations and 206Pb/207Pb ratios at 20-30 cm depth were approximately 6-7 mg/kg and 1.31-1.32, respectively. Assuming an integrated 206Pb/207Pb ratio in deposition over time of 1.18, estimated from lichen measurements and published data for the region, approximately 65% of Pb in the surface (0-1 cm) mineral soil is from anthropogenic sources. Approximately 90% of pollution Pb is found in the 0-10 cm soil layer (Ah) and less than 3% of the pollution Pb is present in the forest biomass and mull-type forest floor combined. Despite low Pb concentrations in vegetation (<2.5 mg/kg), we estimate that between 65 and 100% of the Pb in vegetation and approximately 75% of the Pb in the forest floor is from pollution sources. In total, the pollution Pb burdens at the pine and maple stands are estimated to be 860 and 750 mg/m2, respectively.

Acer↗

Effects of heavy metal pollution on red wood ant (Formica s. str.) populations.

We studied the species composition, mound population densities, relative abundance and colony sizes of red wood ants along a well known air pollution gradient of a copper smelter in Southwest Finland. The dominant species, Formica aquilonia, was further studied for heavy metal (Al, Cu, Cd, Ni, Zn, As, Pb, Hg) levels and morphological characters (body mass, head width, labial gland disease) of workers. We found five species belonging to Formica s. str., and two of them showed changes in their relative abundance, which could not be explained by natural habitat differences. Nest mound volumes were 34% smaller in the polluted area, suggesting that smaller colonies can be maintained there. The heavy metal levels in F. aquilonia workers were higher in the polluted area for all metals, except Hg. The largest relative differences between the study areas (polluted/unpolluted) were found for As (4.1), Ni (2.4), Cu (2.1) and Pb (1.8). Morphological characters of workers were not related to the heavy metal levels. Our data showed that red wood ants can tolerate relatively high amounts of heavy metals and maintain reproducing colonies even in a heavily polluted area, but on the basis of smaller colony sizes, pollution stress may also cause trade-offs in reproduction.

Air Pollutants↗

Amelioration of Indian urban air pollution phytotoxicity in Beta vulgaris L. by modifying NPK nutrients.

Air pollution levels are increasing at an alarming rate in many developing countries, including India and causing a potential threat to crop production. Field experiments were conducted to examine the impact of urban air pollutants on biomass (yield) and some physiological and biochemical parameters of palak (Beta vulgaris L. var. All Green) that grew from germination to maturity at seven periurban sites of Allahabad city having different concentrations of air pollutants under different levels of nutrients. The 6h daily mean NO2, SO2 and O3 concentrations varied from 2.5 to 42.5, 10.6 to 65 and 3.5 to 30.8 microg m(-3), respectively at different locations. Levels of air pollution showed significant negative correlations with photosynthetic pigments, protein, ascorbic acid and starch contents and catalase activity of palak leaves. A significant negative correlation was found for total biomass with SO2 (r=-0.92), NO2 (r=-0.85) and O3 (r=-0.91) concentrations. The increased fertilizer application (N, P and K) over the recommended dose resulted in a positive response by reducing losses in photosynthetic pigments and total biomass. This study proved that ambient air pollution of Allahabad city is influencing negatively to the growth and yield of palak plants.

Air Pollution↗

The reproductive success and quality of blue tits (Parus caeruleus) in a heavy metal pollution gradient.

Studying the effects of pollution on the reproductive performance of birds in the natural environment is of increasing importance due to the need to monitor environmental quality biologically. In this study we investigated the reproductive success and the quality of blue tits in four study sites (at 0.1, 1, 2.5 and 4 km from a metallurgic smelter) located within a heavy metal pollution gradient during three consecutive years. At the two sites closest to the pollution source, a significantly higher proportion of females had egg laying interruptions, and the interruptions also lasted longer (although not significantly). Although the hatching success differed significantly among sites, these differences could not be related to differences in pollution levels. Other important breeding parameters such as the start of egg laying, the clutch size and the breeding success did not differ significantly among sites. Our results suggest that exposure to heavy metal pollution did not influence blue tit reproduction in an important way.

Animals↗

A microcosm test of adaptation and species specific responses to polluted sediments applicable to indigenous chironomids (Diptera).

Chironomids may adapt to pollution stress but data are confined to species that can be reared in the laboratory. A microcosm approach was used to test for adaptation and species differences in heavy metal tolerance. In one experiment, microcosms containing different levels of contaminants were placed in polluted and reference locations. The response of Chironomus februarius to metal contaminants suggested local adaptation: relatively more flies emerged from clean sediment at the reference site and the reverse pattern occurred at the polluted site. However, maternal effects were not specifically ruled out. In another species, Kiefferulus intertinctus, there was no evidence for adaptation. In a second experiment, microcosms with different contaminant levels were placed at two polluted and two unpolluted sites. Species responded differently to contaminants, but there was no evidence for adaptation in the species where this could be tested. Adaptation to heavy metals may be uncommon and species specific, but more sensitive species need to be tested across a range of pollution levels. Factors influencing the likelihood of adaptation are briefly discussed.

Acclimatization↗

Budburst phenology of white birch in industrially polluted areas.

Effects of environmental contamination on plant seasonal development have only rarely been properly documented. Monitoring of leaf growth in mountain birch, Betula pubescens subsp. czerepanovii, around a nickel-copper smelter at Monchegorsk hinted advanced budburst phenology in most polluted sites. However, under laboratory conditions budburst of birch twigs cut in late winter from trees naturally growing around three point polluters (nickel-copper smelter at Monchegorsk, aluminium factory at Kandalaksha, and iron pellet plant at Kostomuksha) showed no relationship with distance from the emission source. In a greenhouse experiment, budburst phenology of mountain birch seedlings grown in unpolluted soil did not depend on seedling origin (from heavily polluted vs. clean sites), whereas seedlings in metal-contaminated soil demonstrated delayed budburst. These results allow to attribute advanced budburst phenology of white birch in severely polluted sites to modified microclimate, rather than to pollution impact on plant physiology or genetics.

Aluminum↗

Altered exoskeleton composition and vitellogenesis in the crustacean Gammarus sp. collected at polluted sites in the Saguenay Fjord, Quebec, Canada.

Gammarus sp. individuals were collected at four intertidal sites subjected to direct sources of pollution (marinas, ferry traffic, and harbors) and at one site with no direct source of pollution. Levels of vitellogenin-like proteins (Vtg), metallothioneins (MT), alkali-labile phosphates (ALPs) in proteins, and lipogenic enzyme activities (i.e., glucose-6-dehydrogenase, isocitrate dehydrogenase, and malate enzyme) were measured in whole soft tissues. In exoskeletons, levels of pH-dependent extractable protein and chitin were determined to assess the possible impacts of pollution on exoskeleton integrity and the molting process. Results show that males were consistently heavier than females regardless of site quality but that the whole-body weight of both sexes was significantly lower at polluted sites. Females displayed either induced or decreased Vtg-like proteins at polluted sites, indicating significant changes in gametogenesis activity. MT levels were not sex dependent and tended to be induced at all impacted sites. ALP levels in acetone-fractionated proteins indicate altered phosphate mobilization at some impacted sites, where females tended to display higher ALP levels. Lipogenic enzyme activities did not vary by sex but were readily increased at impacted sites, suggesting a delay in gonad maturation rates. Exoskeleton protein characteristics revealed that the proportion of chitin in exoskeletons was a lower at most impacted site, suggesting disruption of chitin and pH-dependent protein mobilization. Principal component analysis revealed that gammarids collected at affected sites displayed substantial changes in the proportion of chitin, arthropodin, sclerotin, MTs, and intermediary glucose metabolism (glucose-6-phosphate dehydrogenase, and isocitrate dehydrogenase in soft tissues) and thus suffered from disturbed gametogenesis and exoskeleton integrity.

Animals↗