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At least 1,153 records · Page 64Linked to original sources
PubMed1978

Mechanisms of infertility in genital tract infections due to Chlamydia psittaci transmitted through contaminated semen.

Ten heifers inseminated with semen artificially contaminated with Chlamydia psittaci were not pregnant 40 days later, whereas five of 10 control heifers inseminated with the same semen mixed with control diluent became pregnant. Normal embryos were recovered two and one-half or three days after insemination of another group of animals with semen containing C. psittaci, a finding indicating that fertilization failure was not responsible for the infertility. Uterine biopsy samples taken from two heifers in this group contained scattered Chlamydia-infected cells that were detected by immunofluorescence in the subepithelial tissues of the uterine horns. This finding suggests that the infertility may result from an alteration in the uterine environment caused by multiplication of C. psittaci.

Animals↗
PubMed1979

Chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells.

The growth characteristics of Chlamydia psittaci serotypes 1 and 2 (ovine and bovine origin) were studed in mouse L cells. Formation of inclusions and yield of infectious progeny for serotype 1 were maximal when host cells were treated with cycloheximide and the pH in the cell culture medium was 7.2-7.4. The number of cells that contained inclusions and the infectivity yield for serotype 2 were maximal when the pH was 6.6-7.0. Treatments with diethylaminoethyl dextran and cycloheximide increased inclusion formation in serotype 2 but decreased the yield per infected cell. For both serotypes, centrifugation of the chlamydiae onto monolayers infected 50-1,200 times the number of cells infected with use of stationary adsorption. An enumeration method for L-cell infectious units was developed that gave higher titers than the chicken embryo 50% lethal dose after yolk sac inoculation.

Animals↗
PubMed1985

An epidemic of mild pneumonia due to an unusual strain of Chlamydia psittaci.

An epidemic of mild pneumonia was discovered during a chest radiographic survey of adolescents and young adults in two communities 110 kilometers apart in northern Finland. Antibodies to chlamydial antigens were found in 32 of 34 persons with pneumonitis. Microimmunofluorescence antibody tests suggest that the etiologic agent is closely related or identical to TW-183, an unusual strain of Chlamydia psittaci isolated from the eye of a child in Taiwan. The point prevalence of pneumonitis with antibody to TW-183 in school children at the time of the survey was high-15 and 19 patients per 1,000 students in the two communities. There was no evidence of avian transmission in the epidemic.

Adolescent↗
PubMed1989

Countrywide epidemics of Chlamydia pneumoniae, strain TWAR, in Scandinavia, 1981-1983.

There was a large increase in incidence of reported cases of ornithosis in Norway, Sweden, and Denmark in 1981-1983. Serologic evidence indicated that the epidemic was caused by the newly described Chlamydia organism, C. pneumoniae, strain TWAR. When serum samples that had been shown to contain Chlamydia complement fixing antibodies were tested by the microimmunofluorescence method with TWAR antigen, 49%-71% showed evidence of current TWAR infection during the epidemic years compared with 10%-20% in nonepidemic years.

Adolescent↗
PubMed1991

Diagnosis of pneumonia by cultures, bacterial and viral antigen detection tests, and serology with special reference to antibodies against pneumococcal antigens.

In a prospective study of the etiology of pneumonia 196 adult patients were included. One of the following criteria was required for diagnosis of pneumococcal pneumonia: isolation of pneumococci from blood; isolation from transtracheal aspirate; isolation from sputum or nasopharynx or detection of capsular antigen in sputum in combination with a significant increase in antibodies against at least one pneumococcal antigen (type-specific capsular polysaccharide, C-polysaccharide, pneumolysin); or increase in antibodies against two pneumococcal antigens. Pneumococcal pneumonia was diagnosed in 63 patients (32%). Other diagnoses were nonencapsulated Haemophilus influenzae isolated from transtracheal aspirates, 9; Mycoplasma pneumoniae diagnosed by serology, 17; Chlamydia psittaci, 6; and viral infections, 42. Twenty-two patients (11%) had evidence of infection with more than one agent. The pathogen could not be determined in 70 (36%). Many patients were given antibiotics before admittance to the study, and in some cases a convalescent serum sample was not available.

Adolescent↗
PubMed1996

Localization of chronic Chlamydia psittaci infection in the reproductive tract of sheep.

Twelve sheep experimentally infected with Chlamydia psittaci during pregnancy either aborted or gave birth to weak, low-birth-weight lambs as a result of uteroplacental infection. Subsequently, these ewes excreted chlamydial antigen from their reproductive tracts during estrus. About 1 year after pregnancy failure, immunohistochemistry and polymerase chain reaction were used to examine sections of vagina, uterus, and oviduct for evidence of C. psittaci. Four noninfected control ewes were similarly examined. C. psittaci antigen or DNA was detected in vagina, uterus, and oviduct samples from chronically infected ewes. Endometrial cells in the basal stroma were the predominant site of infection. There was no obvious evidence of pathology associated with persistent infection, but increased numbers of plasma cells and intraepithelial lymphocytes were detected in the uterus. C. psittaci is a naturally occurring reproductive infection in sheep that persists following primary infection. Therefore, sheep provide an excellent model to study the host-parasite interactions that occur during natural infection and subsequent persistence.

Abortion, Veterinary↗
PubMed1983

Susceptibility of Chlamydia psittaci (ovis) to antimicrobial agents.

Minimum inhibitory (MIC) and minimal lethal (MLC) concentrations of 30 antimicrobial agents were determined for field strains and for the A22 EAE vaccine strain of Chlamydia psittaci (ovis). No differences in susceptibility could be detected between strains. The antimicrobials could be divided into four groups. Group A had no effect on growth and development, Group B were bacteriostatic at concentrations in excess of 100 mg/l, Group C were bacteriostatic at concentrations of 10 mg/l or less but were bactericidal at concentrations above 10 mg/l and Group D were bactericidal at concentrations above 0.5 mg/l. The value of antimicrobials in aiding diagnosis and in the therapy of chlamydial abortion of ewes is discussed.

Anti-Bacterial Agents↗