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At least 1,153 records · Page 64Linked to original sources
PubMed1981

Nasal brushing and measurement of ciliary beat frequency. An in vitro method for evaluating pharmacologic effects on human cilia.

Human respiratory tract epithelium suitable for measuring ciliary beat frequency (CBF) may be obtained by nasal brushing. We have used such ciliated epithelium in an in vitro preparation to study the effects on CBF of lidocaine hydrochloride administered topically in vivo before brushing or in vitro after brushing. Beat frequency measured after in vivo administration was unchanged compared to control values while incubating ciliated epithelium in vitro in increasing concentrations of lidocaine resulted in dose-related cilioinhibition. Drug concentrations required to depress ciliary activity in vitro were much higher than those occurring in clinical practice.

Biopsy↗
PubMed1983

Ultrastructural and functional studies of cilia from patients with asthma, aspirin intolerance, and nasal polyps.

"Triad" asthmatics often have sinus disease, nasal polyps, and obstructive airways disease. This presentation is reminiscent of the symptoms of persons afflicted with various forms of ciliary dyskinesia. Therefore, we examined tissues from seven "triad" asthmatic patients for ciliary structural or functional abnormalities. Transmission and scanning electron microscopy revealed no specific abnormalities. Cultures of nasal epithelia were maintained for up to 20 weeks. They showed normal ciliary activity which was not influenced by perfusing the tissue with medium containing aspirin. In contrast, control tissue from a patient with situs inversus and sinusitis showed the expected structural and functional ciliary abnormalities. In culture, the ciliary function of tissue from this patient could be partly restored by perfusion with ATP or ATPase.

Adenosine Triphosphatases↗
PubMed2004

The involvement of cyclic adenosine monophosphate in the control of schistosome miracidium cilia.

This study examined the possible involvement of cyclic adenosine monophosphate (cAMP) in the control of ciliary action of Schistosoma mansoni miracidia. Miracidia immobilized in hypertonic NaCl solution were treated with 3 compounds that are known to increase intracellular cAMP concentrations. Forskolin, at a concentration of 50 microM, induced 50.1% of the miracidia to swim in hypertonic solution. The corresponding values obtained for 3-isobutyl-1-methylxanthine (IBMX) at 1 mM and 8-bromo-cAMP at 10 mM were 42.2 and 50.4%, respectively. The motility-enhancing effect of these compounds was dose dependent. Nevertheless, the swimming speed of miracidia activated in this way was only 10% of that observed in artificial pond water (APW). Cholera toxin had no apparent effect on miracidia swimming in hypertonic NaCl solution. Likewise, swimming in APW treated with forskolin at 50 microM, IBMX at 1 mM, or 8-bromo-cAMP at 10 mM did not induce any apparent change in motility. Miracidia swimming in APW were then treated with 3 compounds that decrease the intracellular concentration of cAMP. MDL-12,330A, at a concentration of 250 microM, caused a dramatic decrease in swimming over a period of 1 hr. Likewise, SQ22536 and imidazole, at concentrations of 20 and 50 mM, respectively, caused 36.5 and 73.4% decreases in swimming under the same conditions. Finally, inhibitors of cAMP-dependent protein kinase, i.e., PKI(14-22)amide, H89, and H88, completely inhibited miracidia swimming in APW at concentrations of 25, 50, and 100 microM, respectively. These results suggest that cAMP and cAMP-dependent protein kinase are involved in osmosis-controlled ciliary motion of schistosome miracidia.

1-Methyl-3-isobutylxanthine↗
PubMedDate not supplied

The effect of carbon dioxide on the activity of cilia. A study on rabbit sinus mucosa in vitro.

The relation of ciliary activity to the partial pressure of carbon dioxide was studied with a photoelectric method on rabbit sinus mucosa in vitro. A dose-response relationship was found, where by ciliary activity was impaired at pCO2 above 5 kPa. The effect of elevated partial pressure of carbon dioxide on ciliary activity in secretory otitis media and sinusitis is discussed.

Animals↗