Joint FAO/WHO expert committee on brucellosis.
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In twenty fattened pigs with papular dermatitis (PD) and in seventeen pigs with no skin lesions (K),--the pigs had the live weight from 95 to 105 kg and came from a D. large fattening house situated in a region with extensive mining (lignite extraction)--we analyzed the skin, muscle, kidneys and liver for the contents of chemical elements (zinc, copper, manganese, lead, cadmium, mercury, chromium) and aflatoxin B1. In pancreas we analyzed only the content of aflatoxin B1. In comparison with the K group, the pigs with PD had in the skin the higher contents of zinc (PD 15.7 +/- 7.1 mg per kg, K 10.1 +/- 1.3 mg per kg, P less than 0.05) and aflatoxin B1 (PD 3.18 +/- 1.53 micrograms per kg, K 1.56 +/- 0.49 microgram per kg, P less than 0.05); in the muscle the higher contents of zinc (PD 30.2 +/- 7.6 mg per kg, K 23.8 +/- 5.4 mg per kg, P less than 0.05), of lead (PD 0.48 +/- 0.31 mg per kg, K 0.25 +/- 0.28 mg per kg, P less than 0.05) and copper (PD 3.19 +/- 3.11 mg per kg, K 0.98 +/- 0.41 mg per kg, P less than 0.01); in the kidneys the higher contents of lead (PD 0.96 +/- 0.75 mg per kg, K 0.26 +/- 0.34 mg per kg, P less than 0.01), cadmium (PD 0.38 +/- 0.14 mg per kg, K 0.26 +/- 0.20 mg per kg, P less than 0.05), manganese (PD 1.14 +/- 0.04 mg per kg, K 0.94 +/- 0.42 mg per kg, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Fecal isolates of enterotoxigenic (44 isolates) and nonenterotoxigenic (25 isolates) Bacteroides fragilis were obtained from diarrheic calves (62 isolates), lambs (2 isolates), and pigs (5 isolates). Using a Microtiter whole-cell agglutination test and gel double-diffusion analysis, the isolates were reacted with nonabsorbed rabbit antisera prepared against 13 isolates of enterotoxigenic B fragilis (ETBF). Isolates of B fragilis were antigenically diverse. Thirty-seven (84%) of the 44 isolates of ETBF comprised 13 serogroups on the basis of reaction in the agglutination test. Fourteen (56%) of the 25 isolates of non-ETBF comprised 4 of the 13 groups. Compared with results of the gel-diffusion test, most isolates had a different agglutination test reaction pattern against the 13 antisera. Isolates of ETBF could not be distinguished from non-ETBF. Antigenic heterogeneity of B fragilis facilitated differentiation of individual isolates, a capability that may be useful in future epidemiologic and virulence studies.
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This study indicates that viable Campylobacter sputorum subsp mucosalis are not present or are present in small numbers in the mucosa of pigs dying of proliferative haemorrhagic enteropathy. The changes present in the mucosa are similar to those seen in pigs recovering from adenomatosis and the evidence obtained indicates that the intracellular organisms observed in this condition are indeed mucosalis. The presence of large amounts of IgA in the altered tissue of both proliferative haemorrhagic enteropathy and porcine intestinal adenomatosis indicates that the failure to recover bacteria may be immunologically mediated but is not simply related to the presence or absence of antibody in the respective conditions.
Reference is made to the third edition of a handbook published 1800 in which experience in preventing and controlling animal diseases is given in the form of standards together with conclusions, the latter being related primarily to the incidence of cattle plague in the second half of the 18th century. The points made in the handbook are related to the history of veterinary medicine, with particular emphasis being laid on today's national veterinary services and the legal provisions which are now in effect for the prevention and control of high risk. Since production relations and productive forces were on a different level then, both the consequences of high-risk animal diseases and action for protection often had deterimental impact upon agricultural production and led to the ruin of many poorer farmers and dependent peasants. The substance of the prevention and control principles was not only adequate, but to some extent is valid even today, notwithstanding the much higher scientific basis on which many problems are tackled in our days. A set of strict rules had been established, and offenders had to expert severe punishment. Yet, in the class society of that time penalty rules differed for 'masters' and 'inferiors'. The handbook is of enlightening importance as a basisfor deliberate application of codes and standards.
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The gastro-intestinal flora of clinically intact piglets on an industrialised sow unit was investigated prior to weaning (n = 12) and after weaning (n = 12). The age of the former group was 23 to 36 days and that of the latter between six and eleven days. While enormous proliferation of haemolysing E. coli was recorded from the anterior portion of the small intestine in the post-weaning group, weaning, quite, generally, was found to have only little impact upon the gastrointestinal flora of piglet. More particularly were there no indicators to the effect that post-weaning proliferation of enteropathogenic strains was favoured by any correlation whatsover between E. coli and other bacterial species or between different types of E. coli. Other factors of possible importance to the pathogenesis of coli-enterotoxaemia are discussed with reference to literature.
Pyelocystitis results from ascending infection of the urinary tract with Corynebacterium suis in sows. Infection occurs regularly during coitus by healthy boars, which are often contaminated with Corynebacterium suis. Characteristic clinical signs of pyelocystitis are haematuria, anemia and emaciation. The whole urinary tract shows severe purulent-necrotic inflammation with progressive vascular lesions. Diagnosis is based on clinical, pathological and bacteriological findings. Distinct changes in urine samples are present. Treatment is only successful in sows with intact function of kidneys. In early detected cases treatment can be effective with repeated application of appropriate antibiotics, e.g. penicillin or ampicillin. There are no proven methods of prevention.
Sodium selenite (encapsulated as doses of 1.4 mg, 2.6 mg and 4.2 mg per kilogram of body weight) was given orally on a daily basis to male weaner pigs, and features of these animals were compared to a control group. Porcine focal symmetrical poliomyelomalacia was produced in all experimental groups between 3 and 20 days after initiation of the treatment. Analysis of blood and several tissues revealed an elevated selenium content for all pigs. Histological lesions in the brain and the cervical lumbar/sacral spinal cord enlargements included endothelial proliferation, neuronal degeneration, microcavitation and glial cell reaction.
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A relatively new technique termed colony blot hybridization appears to be a reliable replacement for the ligated porcine gut loop assay previously used to detect stable toxin-B producing Escherichia coli. The highly reproducible blot assay enables screening of large numbers of isolants for the presence of E coli stable-B toxin genes, thus avoiding variations in phenotypic expression and poor repeatability inherent in the in vivo assays. Availability of this diagnostic test can aid practitioners in identifying enterotoxigenic E coli related disease in swine and in determining the benefits of vaccination programs.
Malignant lymphoma was diagnosed in a 3-year-old, male Sinclair(S-1) miniature pig with acute anorexia, depression, fever, and markedly enlarged inguinal lymph nodes. Results of an initial hemogram indicated a leukocyte count of 121,489 cells/mm3. Most of the leukocytes were mononuclear cells of various sizes, nuclear chromatin pattern, number of nucleoli, amount of cytoplasm, and amount of staining. Cytochemical staining and flow cytometric evaluation of the leukocytes indicated a large number of hypodiploid lymphoblasts in the peripheral blood. Gross necropsy findings included enlargement of all lymph nodes, a pale liver, and multifocal pale areas scattered throughout the kidneys. Microscopic examination indicated massive infiltration of abnormal lymphoid cells into most major organs and complete loss of normal morphologic features of all lymph nodes.
Studied were the therapeutic and prophylactic effects of the preparation Gentavet (containing gentamycin complex II) with pigs, birds and calves affected with colibacteriosis. It was found that in conc. 80,000 IU (0.08 g or 0.8 g) with the drinking water (1 lit.) given 14 days to birds lowered the mortality rate to a higher extent than Avimycin (containing oxitetracycline and neomycin). At the rate of 10,000 IU (0.01 g or 0.1 g) per kg body mass, orally, split in two for 24 h with newborn pigs Gentavet produced a very good prophylactic effect, and when used at a higher rate--16,000 IU (0.016 g or 0.16 g) per kg under the same conditions up to 90 per cent of the pigs with polyenteritis recovered. At 10,000 IU (0.01 g or 0.1 g) per kg body mass, orally, split in two for 24 h with newborn calves with colibacteriosis produced therapeutic and prophylactic effects. Gentamycin complex II residual amounts remained longest in the parenchymal tissue of the kidneys and liver. The withdrawal times in slaughtering birds, pigs, and calves treated with Gentavet should last from 30 to 46 days (31 days for birds, 46 days for pigs and calves of up to 100 kg, and 30 days for heifers of up to 400-500 kg). This period could be shortened 4 to 5 days if both kidneys and liver are to be eliminated after slaughter.
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