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[Experimental study of periosteal osteoblasts coculture with freeze-dried demineralized bone matrix].

OBJECTIVE: To investigate the feasibility of freeze-dried demineralized bone matrix (FDBM) as scaffold material in bone tissue engineering. METHODS: Osteoblasts which were isolated from cranial periosteum of New Zealand rabbits were cultured as the seeding cells, then the cells were cocultured with heterogenous FDBM in vitro. The cell-material complex was observed under phase microscope, light microscope and electronic scanning microscope in order to evaluate the interaction between cells and FDBM. RESULTS: Eight hours after coculture, the osteoblasts adhered to FDBM scaffolds. Seven days later, the osteoblasts differentiated and proliferated in FDBM network. Extracellular matrix was secreted and calcium nodes were formed among osteoblasts. CONCLUSION: FDBM is a good scaffold material for the bone tissue engineering.

Animals↗

[Periosteal desmoid].

The authors describe a rare benign disease occurring in children and adolescents with an increased physical activity at the age of 8-20 years. It is a tumour-like lesion developed by a repeated micro-trauma of the origin of distal fibres of adductor magnus and aponeurotic origin of the medial head of the gastrocnemius. This disease must be paid increased attention as in the region of the knee joint in children it is a location of a frequent development of sarcoma and osteomyelitis. With the knowledge of this lesion such cases may be diagnosed merely by a classic radiograph, preferably in external rotation and CAT and MRI examinations. Of the highest value for the examination is the CAT examination. Radiograph changes are located in the region of the distal part of the medial crest of linea aspera, tuberculum adductorium and posteromedial part of the medial epicondyle of femur.

Adolescent↗

Iliac periosteal graft with vascular pedicle in the treatment of avascular necrosis of the femoral head. Experimental study and clinical application.

From March 1983 to June 1987, 6 patients with avascular necrosis of the femoral head were treated with vascular pedicled iliac periosteum graft. Follow-up for 3 to 7.5 years showed satisfactory results. The patients experienced no hip pain, could walk freely, and resumed work after the treatment. X-ray films showed that the original defect of the femoral head was filled up and its outline was clear and smooth. The bone density of the femoral head and neck normalized. The experimental study indicated that the vascularized periosteum improves the blood supply and initiates the revascularization and osteogenesis of the femoral head. The authors conclude that the technique is suitable for the treatment of avascular necrosis of the femoral head of Stage II and III.

Adolescent↗