[Preservation of bacteria of the genus Acinetobacter in distilled water].
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Nine laboratories participated in a collaborative study of a method for determining 6 nitrosamines, dimethylnitrosamine, diethylnitrosamine, dibutylnitrosamine, nitrosopiperidine, nitrosopyrrolidine, and nitrosomorpholine, in the 5-17 ppb range. The coefficients of variation for repeatability were 10.8, 8.5, 10.4, 8.5, 8.7, and 7.8% with corresponding coefficients of variation for reproducibility of 16.4, 12.0, 13.6, 10.8, 11.2, and 10.3% and recoveries of 89.6, 91.6, 84.7, 90.0, 89.6, and 88.1%, respectively. The method was adopted official first action.
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The effect of occupational exposure on frequency of chromosomal aberrations in workers of coke by-product department was estimated. Fourty seven persons employed in that plant, exposed to tar substances, BaP and dust and 77 non-exposed controls were examined. Concentrations of tar substances, BaP and dust exceeded MAC 13.9, 10 and 1.5 times, respectively. The structural chromosomal aberrations were evaluated in peripheral blood lymphocytes after 72h cultivation, according to a modified Moorhead method. The findings did not revealed any significant differences in the frequency of chromosomal aberrations between the compared groups. These results seem to be produced by a relatively low level of exposure.
The behaviour of 12 strains of gram-negative bacteria was analysed in media having a low content of nutrients. Sterile tap water, de-ionized water, aqua bidestillata and mineral salt solution were used. It was found that Serratia marcescens, Pseudomonas aeruginosa, Enterobacter, Klebsiella and Citrobacter are those with the most frequently observed growth. In the mineral salt solution survived also Providencia, E. coli, Erwinia, Hafnia, and Alkaligenes and reached max. colony numbers of some 100000/ml. When the salts used were added stepwise potassium phosphate and especially magnesium sulfate showed a growth-promoting effect.
The studies were carried out in three coke plants mostly with the use of individual dosimetric methods but also with the stationary method. They revealed the values 2-5 times exceeding the MAC of the tar substances in the air of all workposts, and benzo(a)pyrene at several posts only. The other toxic substances like benzene homologous and benzologous and dust exceeded the MAC value only incidentally so that the workers of this department were less exposed than the people employed directly at the coke production. The ergonomic examinations did not reveal any excess of energetic expenditure or physiological cost. The results of medical studies will be presented in further papers.
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Whiskey produced in illegal stills (ie, "moonshine") remains an important and underappreciated source of lead toxicity in some rural counties of the Southeast. From March 5 through October 26, 1991, eight adult patients with elevated blood lead levels were identified at a rural county hospital in Alabama and were reported to the Alabama Department of Public Health notifiable disease surveillance system. A case-patient was defined as any person 17 years of age or more who presented to the hospital from January 1, 1990, through December 31, 1991, and had a blood lead level of 0.72 mumol/L or more (15 micrograms/dL or more). To identify cases and potential sources of lead exposure, we reviewed medical and laboratory records from the hospital, interviewed patients with elevated blood lead levels, and determined the lead content of moonshine samples. Nine patients met the case definition, including one patient who was not reported to the state. Patients ranged in age from 28 to 62 years; blood lead values ranged from 0.77 to 12.50 mumol/L (16 to 259 micrograms/dL). The most frequent signs of possible lead toxicity included seizures (six), microcytic anemia (five), and encephalopathy (two); one patient died. The only identified source of lead exposure for the nine patients was moonshine ingestion. Moonshine samples available from local stills contained sufficient amounts of lead (340 to 4600 mumol/L) to result in the observed blood lead levels. This investigation emphasizes the adverse health effects and ongoing public health impact of moonshine ingestion.
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