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Blindness and partial-sight: aetiological factors and data collection systems.

271 BD 8 Forms completed by consultant ophthalmologists to enable blind and partially sighted patients to be registered by the social services department were analysed for aetiological factors. This set of data for 1982 to 1986 is tabulated and compared with historical and contemporary data for the country as a whole and some data from Strathclyde, Avon and Leicestershire. It is suggested that in order to monitor epidemiological trends in the causation of visual disability, a composite picture for England could be obtained by requiring one district in each of the fourteen English regional health authorities to collect and analyse the local data. Form BD 8 should be revised, simplified and made computer compatible.

Adolescent↗

An engineer's approach to data collection and planning.

The hospital planner should imitate the engineer in his approach to gathering data on which planning programs must be based. The information must come from all groups involved in the hospital's operation, and each piece of data must then be evaluated for its individual contribution to the overall project.

Administrative Personnel↗

Electropalatographic data collected with and without a face mask.

This study investigates the extent to which the use of a face mask conveys linguopalatal contact changes during speech production. Electropalatographic data from five Catalan speakers were collected for different consonants, i.e., the alveolar stop [n], the alveolopalatal stop [n] and the palatal approximant [j], in the sequences [iCi] and [aCe]. Results for [n] indicate more closure retraction in the mask versus nonmask condition occurring presumably when the mask is pressed forcefully against the face in front of the mouth. The use of the mask for [n] and [j] causes an increase in dorsopalatal contact, which reflects most likely a strategy of articulatory and/or perceptual compensation.

Electric Stimulation↗

Overexpression, purification, crystallization and data collection of Sulfolobus solfataricus Sso6206, a novel highly conserved protein.

Sso6206, a 10.5 kDa protein from Sulfolobus solfataricus, has been overexpressed, purified and crystallized. The protein crystallizes in space group P6(1/5)22, with unit-cell parameters a = b = 157.8, c = 307.3 A. The crystals are hexagonal bipyramids and a data set has been collected to 2.4 A resolution. Molecular replacement cannot be attempted as no convincing model can be identified. Crystals of selenomethionine-variant protein have not yet been obtained. Interestingly, crystal packing, gel filtration and mass spectrometry all suggest the native protein forms a multi-subunit oligomer consisting of >9 subunits.

Amino Acid Sequence↗

Quality of life after first- and second-eye cataract surgery: five-year data collected by the Swedish National Cataract Register.

PURPOSE: To study the benefits of surgery and satisfaction with vision after first- and second-eye cataract surgery in comparable groups of patients. SETTING: Clinics participating in the Swedish National Cataract Outcome Study from 1995 to 1999. METHODS: A prospective observational study evaluated patients' self-assessed visual function and satisfaction with vision before and 6 months after cataract extraction. Patients who had another cataract surgery in the fellow eye during the 6-month study were excluded. Data of 8595 patients having cataract extraction at participating clinics during the month of March for 1995 to 1999 were collected. Preoperative and intraoperative data were reported at the time of surgery. Final postoperative visual acuity was recorded. The Catquest questionnaire was completed before surgery and 6 months after surgery. RESULTS: First-eye surgery was performed in 5570 patients and second-eye surgery, in 3025. Patient-assessed benefits of surgery and satisfaction with vision as defined by the Catquest was higher after second-eye surgery (P <.001). The outcomes were studied in detail in patients with equal visual acuity in the fellow eye before and after surgery and without ocular comorbidity. The better outcome after second-eye surgery was most pronounced in patients with good or intermediate visual acuity in the fellow eye. CONCLUSION: The self-assessed visual outcomes and satisfaction with vision were better after second-eye surgery than after first-eye surgery in comparable groups of patients.

Aged↗

Quantitative infrared spectroscopy of lipids in solution: III. Automated data collection and computer aided calculations of lipid analysis.

In the third part of this series, a data acquisition system is described which digitizes the analog signal from a commercial infrared spectrometer and records the data on paper tape. The paper tape is then processed off-line with a microcomputer and an infrared spectrum is printed. The correction routine for absorbance due to the O-H stretching band is discussed as well as the integration limits for calculation of integrated intensities of the C-H stretching band. The computer program is briefly described and statistical data on correlation between integrated intensity and lipid concentration given.

Autoanalysis↗

Overexpression, purification, crystallization and data collection on the Bordetella pertussis wlbD gene product, a putative UDP-GlcNAc 2'-epimerase.

The Boredetella pertussis wlbD gene product is a putative uridine-5-diphosphate N-acetylglucosamine (UDP-GlcNAc) 2'-epimerase involved in Band A lipopolysaccharide biosynthesis. The wlbD gene is homologous to Escherichia coli rffE (32% identical), an established UDP-GlcNAc 2'-epimerase that is involved in enterobacterial common antigen (ECA) formation. The structure of the rffE protein reveals an unexpected role for a bound sodium ion in orientating a substrate-binding alpha-helix in the enzyme active site. Whilst key active-site residues in rffE are present in the wlbD sequence, the sodium-binding residues outside the active site are absent. This raises questions about the modulation of enzyme activity in these two enzymes. The wlbD gene from B. pertussis has been cloned and overexpressed in E. coli and the resulting protein has been purified to homogeneity. In the current study, crystals of the mutant Gln339Arg wlbD enzyme have been obtained by sitting-drop vapour diffusion. Uncomplexed Gln339Arg and UDP-GlcNAc complex data sets have been collected in-house on a rotating-anode generator to 2.1 A. Combined, the data sets identify the space group as P2(1)2(1)2(1), with unit-cell parameters a = 78, b = 91, c = 125 A, alpha = beta = gamma = 90 degrees. The asymmetric unit contains two monomers and 53% solvent.

Antigens, Bacterial↗

Effect of changing data collection parameters on statistical motor unit number estimates.

The effect of number of samples and selection of data for analysis on the calculation of surface motor unit potential (SMUP) size in the statistical method of motor unit number estimates (MUNE) was determined in 10 normal subjects and 10 with amyotrophic lateral sclerosis (ALS). We recorded 500 sequential compound muscle action potentials (CMAPs) at three different stable stimulus intensities (10-50% of maximal CMAP). Estimated mean SMUP sizes were calculated using Poisson statistical assumptions from the variance of 500 sequential CMAP obtained at each stimulus intensity. The results with the 500 data points were compared with smaller subsets from the same data set. The results using a range of 50-80% of the 500 data points were compared with the full 500. The effect of restricting analysis to data between 5-20% of the CMAP and to standard deviation limits was also assessed. No differences in mean SMUP size were found with stimulus intensity or use of different ranges of data. Consistency was improved with a greater sample number. Data within 5% of CMAP size gave both increased consistency and reduced mean SMUP size in many subjects, but excluded valid responses present at that stimulus intensity. These changes were more prominent in ALS patients in whom the presence of isolated SMUP responses was a striking difference from normal subjects. Noise, spurious data, and large SMUP limited the Poisson assumptions. When these factors are considered, consistent statistical MUNE can be calculated from a continuous sequence of data points. A 2 to 2.5 SD or 10% window are reasonable methods of limiting data for analysis.

Action Potentials↗

Degree of concurrency among experts in data collection and diagnostic hypothesis generation during clinical encounters.

BACKGROUND: Given that there are variations in clinicians' reasoning, methods to elaborate scoring checklists for standardised patient-based assessment need to be valid. The use of data elicited by experts solving problems independently has been advocated as a method of setting performance standards. AIMS: To determine the degree of concurrence and common characteristics among items independently elicited by doctors during patient encounters and to assess the number of experts needed to derive reliable performance standards. METHODS: Six experienced internists worked-up the same 7 chief complaints with standardised patients (SPs). A stimulated recall of the recorded encounter was then performed. The degree of concurrence of the collected history and physical examination information and the generated diagnostic hypotheses was computed. Reliability was derived from generalisability analyses. RESULTS: By case, experts elicited a mean of 114 information items (SD = 15) and generated 30 diagnostic hypotheses (SD = 6). A high concurrence (80-100%) was observed for a mean of 22 information items (20%; SD = 6) and 7 diagnostic hypotheses (24%; SD = 2). More than a third of the 153 highly concurrent information items were clarification questions. At least 3 doctors were needed to obtain a reliability of 0.80 or higher when deriving the scoring checklists. CONCLUSION: The limited concurrency in data elicited by clinicians during a patient encounter supports the use of high-fidelity methods to develop performance checklists used in SP-based assessment. It also suggests that relying only on information collected to assess clinical competence may not be sufficient. Additional criteria, such as structure and style of work-up, should be further explored.

Clinical Competence↗

Statistical issues in analyzing 24-hour dietary recall and 24-hour urine collection data for sodium and potassium intakes.

Dietary recalls and urine assays provide different metrics for assessing sodium and potassium intakes. Means, variances, and correlations of data obtained from these two modes of measurement differ. Pooling of these data is not straightforward, and results from studies employing the different modes may not be comparable. To explore differences between these metrics, the authors used data from the Trial of Nonpharmacologic Intervention in the Elderly (TONE), which included repeated standardized 24-hour dietary recalls and 24-hour urine collections administered over 3 years of follow-up, to estimate sodium and potassium intakes. The authors examined data from 341 control participants assigned to usual care that were collected between August 1992 and December 1995. Dietary recalls yielded estimates of sodium intake that averaged 22% less than those from urine assays and estimates of potassium intake that averaged 16% greater than those from urine assays. Sodium intake estimates were less repeatable (r = 0.22 for diet; r = 0.30 for urine) than potassium intake estimates (r = 0.49 for diet; r = 0.50 for urine), making relations with outcomes more difficult to characterize. Overall, the performance of the two measurement modes was fairly similar across demographic subgroups. Errors in separate estimations of long term sodium and potassium intakes using short term data were strongly correlated, more strongly than the underlying long term intakes of these electrolytes. Because of the correlated measurement error, estimated regression coefficients for linear models including both electrolytes as predictors may be confounded such that the separate relations between these nutrients and outcomes such as blood pressure cannot be reliably estimated by common analytical strategies.

Aged↗

Foodborne infectious risks: do we need a wide system of data collection and survey? The lessons learned from the study of infectious intestinal disease in England.

In 2000, the United Kingdom Government's Food Standards Agency published "A report of the study of infectious intestinal disease in England". This report was the result of over a decade's endeavour and cost well in excess of 2 million pound sterling (approximately 3.3 million euros). The study originated in 1989. In response to national epidemics of foodborne infection with Salmonella enteritidis phage type 4 and Listeria monocytogenes, the Government set up the Committee on the Microbiological Safety of Food (the Richmond Committee). This committee wished to know the actual level of clinical disease in the population giving rise to the laboratory reports of gastro-intestinal pathogens in national surveillance data and recommended studies to achieve this objective. In addition, successors to the to Richmond Committee decided that it would be of value to collect information from both cases and controls, including documenting exposure to biologically plausible risk factors so that differences between the ill and the well could be identified. Enquiries were also made about the clinical course of disease, so that long term sequelae and socio-economic costs could be estimated. A full description of this enormous study is beyond the scope of this paper, which defends the personal view that resources might have been better spent on a less ambitious enquiry.

Case-Control Studies↗

Collecting data along the continuum of prevention and care: a Continuous Quality Improvement approach.

For the past 14 years, a team of applied social scientists and system analysts has worked with a wide variety of Community- Based Organizations (CBO's), other grassroots agencies and networks, and Medical Center departments to support resource, program, staff and data development and evaluation for hospital- and community-based programs and agencies serving HIV at-risk and affected populations. A by-product of this work has been the development, elaboration and refinement of an approach to Continuous Quality Improvement (CQI) which is appropriate for diverse community-based providers and agencies. A key component of our CQI system involves the installation of a sophisticated relational database management and reporting system (DBMS) which is used to collect, analyze, and report data in an iterative process to provide feedback among the evaluators, agency administration and staff. The database system is designed for two purposes: (1) to support the agency's administrative internal and external reporting requirements; (2) to support the development of practice driven health services and early intervention research. The body of work has fostered a unique opportunity for the development of exploratory service-driven research which serves both administrative and research needs.

Acquired Immunodeficiency Syndrome↗

Estimation of growth curves from longitudinal data collected at irregular time intervals.

A general procedure for fitting growth curves is proposed that can be applied to longitudinal data even if observations are missing or irregularly spaced. Maximum likelihood estimates for mean growths are obtained from an EM algorithm. Estimates for standard errors, percentiles, and growth velocities are also produced. The techniques are demonstrated through the use of growth data from a longitudinal study of sickle cell disease.

Algorithms↗

[Sanitary surveillance of workers occupationally exposed to volatile anesthetics: proposal for a computerized program for data collection and processing].

The aim of this work is to present a computerized program for an epidemiological evaluation of the data relevant to workers occupationally exposed to anesthetic gases and of the related occupational risks. The great handiness, the speed of execution, the possibility of carrying out researches by mean of multiple variables correlation and the prompt availability of the data, are the distinguishing features of this program.

Air Pollutants, Occupational↗

Outcome of inborn compared with outborn very low birth weight infants admitted to level 3 Malaysian nurseries. Neonatal Data Collection Group.

To investigate whether a neonatal retrieval system would have any impact on the survival of infants < 1550g birthweight, data from the Malaysian Paediatric Association Very Low Birth Weight (VLBW) study were analyzed. Inborns had a significantly better survival than outborns. Outborn babies had more hypothermia, were more likely to die from hypothermia, received more blood and plasma transfusions, more exchange transfusions, and had more infections. Length of stay was significantly longer for outborns. A neonatal retrieval system could reduce the mortality of VLBW babies as well as reduce length of stay, antibiotic usage, and blood product usage.

Delivery, Obstetric↗

Forecasting cephalosporin and monobactam antibiotic half-lives in humans from data collected in laboratory animals.

The postdistribution half-lives of 10 cephalosporin and 2 monobactam antibiotics in humans were predicted from data obtained in other mammals. This forecasting was accomplished with the allometric equation t1/2 = aWb, where a is the y intercept and b is the slope obtained from the log-log plot of antibiotic half-life (t1/2) versus body weight (W). Dimensionless similarity criteria were used to produce a biological clock for ceftizoxime elimination. The creation of the biological clock, which measured physiologic time (heartbeats) rather than chronologic time (minutes), demonstrated that ceftizoxime half-life was identical in five mammals. This methodology will contribute to infectious disease research through a greater understanding of pharmacokinetic scaling in mammals.

Animals↗

Nutrition data collection in the Multiple Risk Factor Intervention Trial (MRFIT). Baseline nutrient intake of a randomized population.

Selected preliminary baseline nutrient intake data for volunteers participating in the MRFIT study have been presented. Because of the unique selection criteria applied, nutrient intake trends described may not be generalized to the overall population. Nonetheless, present-day trends in this particular group seem to be toward dietary intakes lower in total fat, saturated fat, and dietary cholesterol and higher in polyunsaturated fat than those documented in earlier studies. Thus, utilizing the dietary methodology which has evolved through NHLBI's ongoing program of research investigations, it appears that the health-conscious men randomized into the MRFIT were already consuming diets somewhat more prudent than those in an earlier generation of studies.

Adult↗