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PubMed1980

Studies on cyclic nucleotides in the adrenal gland. X. Effects of adrenocorticotropin and prostaglandin on adenylate cyclase activity in the adrenal cortex.

Effects of ACTH and prostaglandin E1 (PGE1) on adenylate cyclase activities in the zona glomerulosa (the capsular fraction) and the zona fasciculata-reticularis (the decapsulated fraction) from rat adrenocortical glands have been studied. Stimulation by ACTH of adenylate cyclase activity was observed in both the capsular and decapsulated fractions in a similar dose-dependent manner. PGE1 only stimulated adenylate cyclase activity in the capsular fraction. The maximal stimulations induced by ACTH and PGE1 were additive on the capsular enzyme. A prostaglandin antagonist, polyphloretin phosphate failed to inhibit ACTH stimulation of adenylate cyclase at a concentration which completely blocked the effect of PGE1. [3H]PGE1 bound to subcellular fractions of both the capsular and decapsulated fractions of the gland. These results suggest that the capsular fraction possesses receptors for PGE1 coupled to the adenylate cyclase system which are distinct from those for ACTH. On the other hand, PGE1 receptors in the decapsulated fraction seem not to be coupled to the adenylate cyclase system.

Adenylyl Cyclases↗
PubMed1984

Studies on cyclic nucleotides in the adrenal gland. XI. Adrenergic regulation of adenylate cyclase activity in the adrenal cortex.

Adrenergic regulation of adenylate cyclase activities in the zona glomerulosa (the capsular fraction) and the zona fasciculata-reticularis (the decapsulated fraction) from rat adrenocortical glands has been investigated. Specific binding of [3H]dihydroalprenolol to the membrane from the capsular and the decapsulated fractions was saturable with dissociation constant (Kd) of 4.67 and 5.1 microM, respectively. The receptor density in the capsular and the decapsulated fractions was 230 and 235 fmol/mg protein, respectively. The potencies which isoproterenol, epinephrine, salbutamol, and norepinephrine competed with [3H]dihydroalprenolol binding sites indicted that adrenergic receptors of the capsular and the decapsulated membranes were of the beta 2-type. beta-Adrenergic stimulation of the adenylate cyclase system was observed only in the capsular fraction. This suggests that beta-adrenergic receptors of the capsular membrane are associated with their adenylate cyclase system, but those of the decapsulated membrane are not. Maximum stimulatory concentrations of ACTH and isoproterenol had no additive effect on the capsular adenylate cyclase, indicating that receptors for ACTH and beta-adrenergic agonists are coupled to a common pool of the cyclase.

Adenylyl Cyclases↗