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An evaluation of the role of suction rectal biopsy in the diagnosis of intestinal neuronal dysplasia.

BACKGROUND: German pathologists have developed a consensus for histological features of intestinal neuronal dysplasia. METHODS: A blind reevaluation of ganglionic suction rectal biopsies from infants and children who initially presented with symptoms of intestinal dysmotility was made. RESULTS: 84 of 411 specimens had sufficient depth of submucosa for adequate assessment. Questionnaires or clinical interviews were employed 3-5 years after biopsy in these 84 patients to assess the relationship between histological changes and persistent symptomology. Eighteen children were lost to follow-up, 4 others had Hirschsprung's disease the study biopsy specimen having been taken from the pulled-through bowel after surgical resection of the aganglionic segment. The remaining 62 patients were divided into three groups. There were six patients in group A (both obligatory criteria) and 28 in group B (nonessential, or just one of the obligatory criteria), and 28 in group C (normal appearances). On follow-up, two of the 28 (7%) in group B, and six of the 28 (21%) in group C had persistent dysmotility symptoms. CONCLUSIONS: Histological criteria of the consensus of German Pathologists for intestinal neuronal dysplasia was unhelpful in predicting the clinical outcome and therefore, should not influence clinical management. As one of the obligatory criteria, hyperplasia of the submucosal plexus was significantly more common in neonates (< 4 weeks), it is concluded that this is an age-related variation.

Acetylcholinesterase↗

Effect of short-duration hyperventilation during endotracheal suctioning on intracranial pressure in severe head-injured adults.

A repeated measures randomized within-group design was used to determine the effectiveness of controlled short-duration hyperventilation (HV) in blunting the increase of intracranial pressure (ICP) during endotracheal suctioning (ETS). A multimodal continuous real-time computerized data acquisition procedure was used to compare the effects of two HV ETS protocols on ICP, arterial pressure, cerebral perfusion pressure (CPP), heart rate, and arterial oxygen saturation in severe head-injured adult patients. The results indicated that short-duration HV for 1 minute, which decreases the PaCO2, reduced ETS-induced elevations in ICP while maintaining CPP. However, it is not clear whether short-duration HV is neuroprotective, particularly in ischemic regions of the brain. Therefore, before a change in practice is implemented on the use of short-duration HV as a prophylactic treatment against ETS-induced elevations in ICP, additional questions on cerebral oxygen delivery and uptake need to be answered.

Adolescent↗

Create a low-cost tracheotomy model for suctioning simulation.

Use of simulation for nursing skill development can provide effective learning while minimizing risk to patients. However, simulators are expensive and portability can be limited. The author describes how to create a low-cost model for simulating tracheotomy suctioning and care. Construction is simple, with estimated materials cost for making the model less than 100 dollars.

Audiovisual Aids↗

Hazards of delivery room resuscitation using oral methods of endotracheal suctioning.

Between 1978 and 1982 at least eight physicians at our institution were exposed to infectious secretions while performing mouth-to-tube resuscitation. A questionnaire revealed that 74% of responding pediatric physicians accidentally ingested secretions in 1982. The risk of cross-contamination between newborn patients and resuscitating physicians with the oral methods of oropharyngeal and endotracheal suctioning is considerable.

Adult↗

Suction curettage to remove excess fat for body contouring.

We have significantly expanded the use of a lipectomy procedure which heretofore had been restricted to the removal of strictly localized excess fat deposits by curettage through a small incision. By modifying the suction curette and utilizing it for extensive body contouring, we have been successful in correcting relatively major fat deformities without causing any postoperative surgical deformities. All our results to date have been satisfactory. This procedure may be helpful to those surgeons who have been seeking a simplified method for removing excessive fat without extensive scarring. The improvement in body contour is often beyond the preoperative expectations of both the surgeon and the patient.

Adipose Tissue↗

Face and neck suction-assisted lipectomy associated with rhytidectomy.

A technique is described in which suction-assisted lipectomy is used for excessive fat removal in association with the standard rhytidectomy. It is safe, reliable, and can be performed through a small horizontal submental incision. A more natural appearing neck is produced by adjusting the amount of fat removed. Areas previously considered dangerous for excision because of possible damage to branches of the facial nerves, such as the cheek areas, jowls, parotid, and nasolabial folds, can now be approached.

Adipose Tissue↗

Skin sensation after suction lipectomy: a prospective study of 50 consecutive patients.

Using a reproducible pain stimulus, skin sensation was evaluated in a prospective study of 50 consecutive patients who underwent suction lipectomy. A total of 294 anatomic areas were evaluated and treated. Before operation, many patients were found to have patchy areas of decreased sensation, a condition that was unknown to them. Initially after surgery, all patients had decreased sensation in the treated areas. However, sensation usually returned to normal in 6 to 8 months. In some patients, sensory return to preoperative levels took as long as 1 year, and in a few, patchy areas of decreased sensation were present even longer. In general, the larger the treated area, the larger are the area and degree of sensory loss and the slower is the return of sensation.

Adipose Tissue↗

Lens surgery with the vitreous infusion suction cutter.

The vitreous infusion suction cutter was used in the removal of congenital and adult cataracts or lens remnants. The instrument may be used through a standard corneoscleral incision or through the pars plana. When a hard nucleus cannot be dispersed, ultrasonic phacofragmentation may be of help. Several brief case illustrations demonstrate the effectiveness of this instrument in lens surgery.

Adolescent↗

Cerebrovascular response of closed head-injured patients to a standardized endotracheal tube suctioning and manual hyperventilation procedure.

The purposes of this research were to assess the cerebrovascular response of closed head-injured patients to a standardized endotracheal tube suctioning/manual hyperventilation (ETTS/MH) procedure and determine if a 5-minute rest period, following the ETTS/MH procedure, was sufficient in length to allow cerebrovascular parameters to return to pre-intervention levels. The sample consisted of 49 head-injured patients, 18 with a baseline mean intracranial pressure (MICP) of 7.1 mm Hg, standard error (SE) .6, and 31 with a baseline MICP of 9.3 mm Hg, SE .6. A total of 145 ETTS/MH procedures were completed, 51 within the lower baseline MICP group and 94 within the higher baseline MICP group. All data were collected within 72 hours of injury using a bedside component of the Hewlett-Packard Patient Monitoring System (H-P PMS). Computer programs written specifically for this research recorded physiologic data at specified points during baseline, throughout the ETTS/MH procedure and during the recovery period. A one-way repeated measures analysis of variance (ANOVA) was used to examine whether significant differences occurred over time during the baseline, intervention and recovery periods for each dependent variable. Significant F statistics were followed by nonorthogonal, multivariate planned comparisons using the Bonferroni posthoc test to identify significant differences between baseline and each 15 second measurement of all physiologic variables during the ETTS/MH procedure and at the end of each recovery minute over a 5-minute period. Independent t tests were used to assess between group differences at each data point throughout the procedure. Findings indicated those patients whose baseline MICP was most elevated demonstrated significantly (p less than .05) lower mean arterial blood pressure, cerebral perfusion pressure, and heart rate in response to the ETTS/MH procedure when compared to those patients with a lower baseline MICP. Additionally, 60 seconds of MH was required in both patient groups following the second and third suctioning catheter pass in order to reverse a step-wise increase in MICP. Finally, at least 2 full minutes of recovery time following the completion of the ETTS/MH procedure were required to allow all measured physiologic variables to return to baseline.

Adolescent↗

Measurement of skin elastic properties with a new suction device (I): Relationship to age, sex and the degree of obesity in normal individuals.

We measured skin elastic properties (distension ability, retraction ability, and the skin elasticity) of 4 different body sites, finger, hand, forearm, and chest, in 191 normal individuals by means of a new suction device and investigated the correlation of skin elastic properties with sex, site, age, and the degree of obesity. Neither sex nor the degree of obesity were correlated with skin elastic properties. The skin elastic property of the chest was significantly higher than those of the other three sites. Decrease in the skin elasticity of the hand, forearm and chest were associated with aging. This simple and non-invasive device can provide an objective method for evaluating skin elastic property.

Adolescent↗

A simple suction device.

A simple suction device is described, suitable for portable resuscitation kits. It can be made cheaply, from parts already available in most hospitals and is reliable and effective.

Equipment Design↗

High levels of hyaluronate in suction blister fluid from active psoriatic lesions.

Hyaluronate concentrations were measured in suction blister fluid from blisters raised on the abdominal skin of 12 healthy control subjects, on unaffected skin of 14 patients with psoriasis, and on lesional skin from 12 of these patients. The concentrations of hyaluronate in blister fluid from the controls and from the uninvolved skin of patients with psoriasis were within the same range (2.9-10.5 and 2.4-7.8 mg/l, respectively). Six of the patients had active, widespread, untreated psoriasis and in these cases the hyaluronate concentration in blister fluid from lesional skin was greatly increased (24-30 mg/l). Patients with stable or regressing psoriasis had no significant increase in hyaluronate levels in the blister fluid from lesional skin compared with the controls (range 4.6-12.4 mg/l). Patients with active psoriasis had significantly higher serum hyaluronate concentrations than the controls and those with inactive psoriasis.

Adult↗

Increased tryptase levels in suction-blister fluid from patients with urticaria.

The levels of tryptase in the suction-blister fluid from patients with chronic urticaria, urticaria pigmentosa, cholinergic urticaria, urticarial dermographism, prurigo of unknown origin, eczema, psoriasis, atopic dermatitis, and from healthy controls were studied. The blister fluid from controls contained up to 15 micrograms/l of tryptase, whereas that from patients with active urticaria contained greater than 50 micrograms/l. This study demonstrates that patients with urticaria have mast cells that readily release tryptase in both the lesional and non-lesional areas of skin.

Adult↗

Proteomic analysis of suction blister fluid isolated from human skin.

The regulation of cutaneous immune responses in health and disease is mediated locally by proteins such as cytokines and chemokines. We used a novel approach involving proteomic profiling of fluid drawn from suction blisters to compare and contrast protein expression in normal skin with that in nonlesional skin from a patient with plaque psoriasis. We also examined the impact of exogenous interleukin-1beta, a proinflammatory cytokine, on protein expression in these tissues. Described here are the results of proteomic profiling of 670 proteins from blister fluid, and the identification by differential expression of nine proteins between one volunteer with psoriasis and one normal volunteer. Although the apparent disease association of these nine proteins will require validation using additional volunteers, the identification of candidate protein biomarkers through proteomic analyses of blister fluid represents a promising approach for monitoring the disease activity and efficacy of therapeutic intervention in human skin diseases.

Biomarkers↗