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[Consequences of abnormalities of chromosome structure in domestic animals].

Abnormalities in chromosome structure generally have no phenotypic expression but are very often associated with reproductive disorders. In cattle, sheep and goats, the robertsonian translocation seems to be the most frequent abnormality of chromosome structure. In the pig, reciprocal translocations are very common. The accumulation of data on the frequency of such abnormalities and their effects on reproductive performance prompted an evaluation of their economic consequences in cattle and pigs. In cattle, because of the negative effect of 1/29 translocation, an eradication program, based on the removal of carrier bulls from artificial insemination centers was established. In pig, the main effect of the reciprocal translocations was a reduction in the number of offspring, up to 50%, thus representing a considerable economic loss.

Animals↗

[The ultrastructure of sterile larvocysts of Echinococcus granulosus from different hosts].

The sterile larvocysts of Echinococcus granulosus are characterized by host-dependent morphological variability. The distinctions reside in the intensity of germinative layer development, glycogen and lipid content, ultrastructure of the tegument distal cytoplasm. The larvocysts obtained from one host species in different geographic zones of the country are morphologically similar. The sterile Echinococcus granulosus larvocysts obtained upon experimental infection of mice demonstrate typical morphological signs of the strain used for infection.

Animals↗

[Diseases of the extremities of swine in relationship to the floor condition with special reference to bursitis].

In 2 pig farms, 102 sows kept in single ranging on plain concrete floor, 168 sows kept in single ranging on cast-iron grates and 27 fattening pigs kept in group penning on concrete slatted floors were repeatedly examined for the incidence of auxiliary synovial bursae (ASB). The clinical examination was completed by radiological, patho-anatomical and histological examinations of the rear legs. In sows, ASB were found on two prominences of the bones: on the latero-plantar area of the calcaneum and the fourth tarsal bone as well as on the plantar tarsal sesamoid bone. ASB on the rear legs were found in 41.2% of the sows kept on plain concrete floor and in 59.5% of the sows kept on cast-iron grates. The ASB of the sows kept on cast-iron grates were larger than those of the sows kept on plain concrete floor. In 49% of the sows kept on plain concrete floor the skin covering the ASB was reddened, in 45% of the sows kept on cast-iron grates it was covered with scab. Injury and necrosis of the skin as well as injury of the ASB themselves were found only in animals kept on cast-iron grates and made up 5% of the cases. In 30% of the cases, palpation of the ASB induced defensive movement. The palpation of ball-shaped, soft bursae more often caused defensive movement than did the palpation of flat-shaped, hard bursae. The occurrence of ASB was combined with kyphosis of the spine, false posture of the rear legs, dystasia, hyperextension of the fetlock, arthrosis of the ankle joint, and anomalies of the claws. In fattening pigs, the ASB were localized at the lateroplantar area of the calcaneum and the fourth tarsal bone as well as at the posterior surface of the calcaneum. At the end of the fattening period, ASB were detected in 26 out of 27 fattening pigs. All of the 48 ASB that were examined histologically showed signs of inflammation. In 30 cases the inflammation was chronic, in 18 cases it turned out to be florid. The results indicate that ASB cause pain during the developing phase and also in the chronic stage.

Animals↗

Characterization of an attenuated strain of Actinobacillus pleuropneumoniae, serotype 1.

Pleuropneumonia is an important disease of swine caused by Actinobacillus pleuropneumoniae. Putative virulence determinants include capsule, lipopolysaccharide, and cytotoxin. We studied the virulence and virulence determinants of 2 strains: CM5 and CM5A of serotype 1. Strain CM5 was isolated from a pig with pleuropneumonia and passaged once in vitro; strain CM5A was a substrain of CM5 passaged 70 times in vitro. Pigs challenge exposed to an aerosol of 1.3 x 10(7) colony-forming units of CM5/ml died within 30 hours; pigs challenge exposed to an aerosol of 1.6 x 10(8) colony-forming units of CM5A/ml survived. The average thickness of the capsular layer was 137 nm in strain CM5 and 53 nm in strain CM5A in bacteria treated with homologous antibody and examined by transmission electron microscopy. Similarly, capsular material binding polycationic ferritin was found in colonies of strain CM5, but not in strain CM5A. The ratio of hexosamine to protein in extracted capsule of CM5 was more than twice that of CM5A. The polyacrylamide gel electrophoretic profile of the lipopolysaccharide, outer membrane proteins, and whole cell proteins did not differ between the 2 strains. Also, the amount of cytotoxin or endotoxin produced by the 2 strains during the logarithmic growth phase was not different. The electrophoretic profile of restriction endonuclease digested DNA was similar, with the exception of bands in the 750- and 620-basepair regions. It was concluded that attenuation of strain CM5A during in vitro passage was a result of reduced capsule production and that encapsulation is an important virulence determinant of A pleuropneumoniae, serotype 1.

Actinobacillus↗

A study on the life-cycle and epidemiology of Pseudanoplocephala crawfordi Baylis, 1927.

A study of the life-cycle and epidemiology of Pseudanoplocephala crawfordi Baylis, 1927 is reported. Intermediate hosts are Tenebrio obscurus, T. molitor, Carpophilus dimidiatus and Tribolium castaneum. The first three species are new host records. Development in the intermediate host takes 1-1.5 months. The completion of the life-cycle takes 2-2.5 months. Surveys of intermediate hosts, pigs, humans and rats were carried out. In Rattus norvegicus, the prevalence was as high as 22%. Epidemiological factors and suggestions for control measures are discussed.

Animals↗

A sequential light microscopic study of the pulmonary lesions in porcine dermatosis vegetans.

The sequential pulmonary changes in dermatosis vegetans were studied in pigs aged one, seven, 14, 17, 21 and 28 days, and two and a half, four and 12 months. At one day old the lung tissues appeared normal. At seven days old there was a moderate infiltration of mononuclear inflammatory cells in the alveolar structures, and proliferation of type II pneumocytes was also observed. At 14 and 17 days old the alveolar structures were intensely infiltrated with mononuclear inflammatory cells. At 21 days old, loosely arranged epithelioid and multinucleate giant cell granulomas appeared, and there was a proliferation of alveolar epithelial cells. By four and 12 months old the inflammatory changes diminished, and small granulomas and moderate alveolar fibrosis were present. The initial lesions were characterised by an alveolitis, developing into loosely arranged granulomas by four months old, and persisting to the age of 12 months. These observations suggest that the interpretation of the lung changes and the disease in general should be revised.

Animals↗

[Lectin agglutination for the characterization of intestinal treponema isolates from different animal species].

The study presents the results of lectin-associated agglutinations of intestinal strains of treponemes isolated from pigs, dogs, mice and rats. At all 95 isolates are investigated, within the type strains for Treponema hyodysenteriae serotype 1-4 and Treponema innocens. Reactions with Concanavalin A and the Limulus-polyphemus-Lectin are often seen (twenty lectins used). Three out of four type strains for Treponema hyodysenteriae show an identical pattern of reaction, which is often seen in Treponema strains from dysentery suspected cases, too. Basing on these data a ranging in groups of lectin agglutination is proposed.

Agglutination Tests↗

Evaluation of a monoclonal antibody to the K99 fimbrial adhesin produced by Escherichia coli enterotoxigenic for calves, lambs and piglets.

All the K99+ Escherichia coli grown at 37 degrees C stained strongly with a peroxidase labelled K99 monoclonal antibody using a direct immunoperoxidase staining procedure. There was no reaction when these bacteria were cultured at 18 degrees C or when K99- E coli were grown at either temperature. The binding of the monoclonal antibody to K99 antigen was inhibited by OK antisera to heterologous K99+ E coli but OK antisera to E coli producing adhesins other than K99 were without effect. Using the slide agglutination test the reactions of the monoclonal antibody were identical to those of a polyclonal antiserum to K99 when both were used in parallel to examine 100 K99+ E coli from at least 10 somatic O groups and 1308 K99+ E coli from at least 82 different somatic O groups submitted for routine serological typing in England or the, USA. The monoclonal antibody reacted with K99+ E coli in cryostat sections of the ileum from a piglet infected with E coli strain B44 (O9: K30, K99, F41) but there was no reaction with similar material from piglets infected by E coli strains 1751 (O101: F41), X177/81 (O9: K103, 987P) or Abbotstown (O149: K91, K88ac).

Adhesins, Escherichia coli↗

Oesophagogastric parakeratosis in the growing pig: effects of the physical form of barley-based diets and added fibre.

In four experiments a total of 288 individually fed pigs were given barley-based diets for about 100 days from about 20 kg liveweight. Fine grinding of barley increased the number and severity of oesophagogastric lesions. Pelleting a diet based on coarsely ground barley had a similar effect. Coarser grinding of the barley and substituting small proportions of oat husk, but not of bran, gave lower incidences and severities of lesions. The performance responses of the pigs differed and give a framework for deciding on the balance to be struck between optimal performance and the risk of lesion development.

Animal Feed↗

An unusual localization of developmental stages of Cryptosporidium parvum Tyzzer, 1912 in the cells of small intestine of a gnotobiotic piglet.

In a gnotobiotic piglet infected with 50.10(6) oocysts of Cryptosporidium parvum, developmental stages of the parasite were found in the duodenum, anterior jejunum and middle jejunum on the first day post infection (1 DPI). During the studies of the ultrastructure, trophozoites and meronts of C. parvum were encountered in the microvillous zone of enterocytes, and the outer membrane of parasites was a continuation of the membrane of microvilli. Unusual was the attachment of C. parvum trophozoite on the opening of a goblet cell, as well as a meront localized in the cytoplasm in the enterocyte of anterior jejunum. These findings show that C. parvum is very adaptive and capable of developing in a still undescribed location.

Animals↗

Porcine focal symmetrical poliomyelomalacia: test for an interaction between dietary selenium and niacin.

Experiments were conducted to test the hypothesis that dietary supplementation with nicotinamide would retard or eliminate the signs of selenium induced porcine focal symmetrical poliomyelomalacia (PFSP). Mixed-sex feeder pigs, approximately five weeks old, were divided into four groups and daily received, by oral capsule, the following treatments: no supplementation (control); 2.86 mg sodium selenite per kg body wt (selenium only); 44 mg nicotinamide per kg body wt (niacin only); or both the niacin and selenium (niacin + selenium). Over the ten day treatment body weights and behavior scores were recorded, as well as collection of fluid (blood, serum, urine) samples. Upon death, tissue samples (kidney, liver, brain, spinal cord and muscle) were obtained. All of these samples were analyzed for total selenium and bioactive niacin compounds. After gross pathological analysis, 11 samples from specific brain and spinal cord regions were taken for fixation and processing for histological analysis by light microscopy. The selenium only group showed behavior signs related to PFSP after two days of treatment with the average time of death at 6.5 days. Tissue levels of selenium were elevated and histological analyses established the expected lesions of PFSP. No disorders were noted in the control and niacin only groups. The niacin + selenium groups had slightly retarded changes in behavior scores (first differences from controls on day 4) but their mean day of death (7.5 days of treatment) did not differ from that of the selenium only groups. Histological analyses of these tissues revealed similar lesions to the selenium only group, but they may have been of lesser magnitude. The data were consistent with, but only partially supportive of, the above hypothesis.

Animals↗

[Animal reservoir hosts of Trypanosoma brucei gambiense in Zaire: trypanosome infections in two foci in Bas-Zaire].

The prevalence of Trypanosoma spp. infections in domestic animals was estimated in a forest (Boma) and a savanna (Kimpese) sleeping focus in Bas-Zaire. The miniature anion-exchange centrifugation technique was used to determine the infection rates with T. congolense, T. vivax and T. brucei spp. in 505 animals. T. congolense predominated in both foci with the highest prevalence in pigs (76.2%), followed by sheep (31.3%), dogs (30.6%) and goats (7.4%). T. vivax was seen only on two occasions. In the forest zone, T. brucei spp. infections were frequent (pigs 16.5%, sheep 6.2%, dogs 3.4%, goats 1.1%) in contrast to the savanna area where only one T. brucei spp. infection was diagnosed. Twenty five primary isolations of T. brucei were done using different isolation and stabilization approaches. Isolates and stocks await behavioural, biochemical and molecular biological identification to discriminate T. b. brucei and T. b. gambiense of domestic animal origin.

Animals↗

[The differentiation of Bordetella (B.) bronchiseptica strains. 8. The protective behavior of B. bronchiseptica strains in mice in relation to the loss of K factors].

K factors are phase-dependent, instable antigen components of B. bronchiseptica. Loss of K factors was induced by passaging on 10% horse blood agar. All K factors were not simultaneously eliminated. Cultures obtained from various passages were checked on white mice by Lienotox testing and in protectivity experiments. Culturing of B. bronchiseptica on 10% horse blood agar in Phase I probably had an adverse impact on expression of virulence-dependent factors. K factor expression in B. bronchiseptica was found to depend on both phase condition and culturing medium.

Animals↗