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At least 1,099 records · Page 61Linked to original sources
PubMed2003

FHL3 is an actin-binding protein that regulates alpha-actinin-mediated actin bundling: FHL3 localizes to actin stress fibers and enhances cell spreading and stress fiber disassembly.

Four and a half LIM domain (FHL) proteins are members of the LIM protein superfamily. Several FHL proteins function as co-activators of CREM/CREB transcription factors and the androgen receptor. FHL3 is highly expressed in skeletal muscle, but its function is unknown. FHL3 localized to the nucleus in C2C12 myoblasts and, following integrin engagement, exited the nucleus and localized to actin stress fibers and focal adhesions. In mature skeletal muscle FHL3 was found at the Z-line. Actin was identified as a potential FHL3 binding partner in yeast two-hybrid screening of a skeletal muscle library. FHL3 complexed with actin both in vitro and in vivo as shown by glutathione S-transferase pull-down assays and co-immunoprecipitation of recombinant and endogenous proteins. FHL3 promoted cell spreading and when overexpressed in spread C2C12 cells disrupted actin stress fibers. Increased FHL3 expression was detected in highly motile cells migrating into an artificial wound, compared with non-motile cells. The molecular mechanism by which FHL3 induced actin stress fiber disassembly was demonstrated by low speed actin co-sedimentation assays and electron microscopy. FHL3 inhibited alpha-actinin-mediated actin bundling. These studies reveal FHL3 as a significant regulator of actin cytoskeletal dynamics in skeletal myoblasts.

Actinin↗
PubMed1981

Measurement of zinc flux through plasma in normal and endotoxin-stressed pigs and the effects of Zn supplementation during stress.

1. The rates of transport of zinc through plasma have been investigated in normal and endotoxin-stressed pigs. 2.65Zn added to porcine plasma in vitro rapidly equilibrated with the Zn originally present. 3. 65 Zn bound to albumin and injected intravascularly into pigs rapidly equilibrated with two kinetically distinguishable pools. The first of these pools was mainly associated with the plasma but was significantly larger than the plasma volume and substantially so in Zn-deficient pigs. The second pool appeared to represent a summation of the rapidly-exchangeable Zn within the extravascular tissues. 4. In non-stressed animals, the flux of Zn from the plasma of Zn-deficient pigs was only half that in Zn-supplemented animals. 5. Administration of endotoxin reduced the plasma Zn concentration of Zn-supplemented pigs but not of Zn-deficient animals. The fractional turnover rates of Zn were not altered in eight of the two pools following endotoxin. 6. At 10 h after giving endotoxin neither the Zn content of the two pools nor the flux of Zn through them differed significantly between Zn-deficient and control pigs. 7. Intravascular infusion of Zn at the rate which essentially prevented the usual depression in plasma Zn concentration following endotoxin failed to alleviate the effects of endotoxin on Zn-supplemented pits. 8. The reduction in plasma Zn concentration following endotoxin stress appears to be a normal physiological response which is not indicative of an increased metabolic requirement for Zn.

Animals↗
PubMed2003

Influence of stress protein CSP 310 and antiserum against this protein on oxygen uptake, lipid peroxidation, and temperature of winter wheat seedling shoots during cold stress.

It is determined that infiltration of winter wheat seedling shoots by anti-CSP 310 antiserum caused a significant decrease of oxygen uptake in winter wheat shoots during short-term cold stress. On the other hand, infiltration of winter wheat seedling shoots by stress protein CSP 310 caused an increase of oxygen consumption. The comparison of the influence of infiltration of winter wheat shoots by CSP 310 and anti-CSP 310 antiserum on the rate of lipid peroxidation showed that, if infiltration by CSP 310 caused a decrease of conjugated diene formation, infiltration by anti-CSP 310 antiserum did not cause any significant changes in the rate of lipid peroxidation. The study of the influence of infiltration of winter wheat shoots by CSP 310 and anti-CSP 310 antiserum on the temperature of winter wheat shoots during cold stress showed that CSP 310 caused the increase of their temperature while anti-CSP 310 caused a decrease of their temperature.

Cold Temperature↗
PubMed2002

ROS, stress-activated kinases and stress signaling in cancer.

Anticancer therapy is frequently efficient in early stages of the disease, whereas advanced tumors are usually resistant to the same treatments. The molecular basis for this change is not entirely understood. Many anticancer agents are DNA- or cytoskeleton-damaging drugs that show some specificity towards dividing cells. However, recent studies show that these agents also activate stress-signaling cascades that may play a role in eliciting the observed therapeutic effects. We discuss recent findings that suggest that induction of stress signaling in oncogenically transformed cells is integrated into apoptotic pathways. Reactive oxygen species (ROS) and stress-activated protein kinases (SAPKs), which are potentiated in recently transformed cells, emerge as key effectors of cell death. In advanced tumors, however, these agents are downregulated and, consequently, death signaling is suppressed. Such changes in ROS and SAPK activity levels during the course of tumor development may underlie the changes in responsiveness to anticancer therapy.

Animals↗
PubMedDate not supplied

Posttraumatic stress disorder (PTSD) and posttraumatic stress symptoms (PTSS) in families of adolescent childhood cancer survivors.

OBJECTIVE: To describe rates and concordance of posttraumatic stress disorder (PTSD) and posttraumatic stress symptoms (PTSS) in adolescent childhood cancer survivors and their mothers and fathers. METHOD: Participants were 150 adolescent survivors of childhood cancer, 146 mothers, and 103 fathers who completed the Impact of Events Scale-Revised, the Posttraumatic Stress Disorder Reaction Index, and the PTSD module of the Structured Clinical Interview for the Diagnostic and Statistical Manual of Mental Disorders, fourth edition. RESULTS: PTSS are common in families of childhood cancer survivors. Parents reported more symptomatology than former patients. Mothers and fathers had relatively equal rates of current PTSD and levels of PTSS. Nearly 30% of mothers met diagnostic criteria since their child's diagnosis, with 13.7% currently experiencing PTSD. Nearly 20% of families had at least one parent with current PTSD. Ninety-nine percent of the sample had at least one family member reexperiencing symptoms. CONCLUSIONS: Both PTSD and PTSS help in understanding the experience of adolescent cancer survivors and their families. Within families of childhood cancer survivors, it is likely that some member may be experiencing treatable bothersome memories, arousal, or avoidance specific to the cancer experience.

Child↗
PubMed1996

Adaptation to repeated restraint stress in rats: failure of ethanol-treated rats to adapt in the stress schedule.

Adaptation to a repeated restraint stress schedule was monitored in ethanol-treated and control rats. A single episode of 2 h restraint decreased food intake in both control and ethanol-treated rats. The decreases in control rats were not observed following the 5th daily restraint of 2 h/day, suggesting that adaptation has occurred. Ethanol-treated rats, however, exhibited decreased food intake even after 5th daily restraint of 2 h/day. Ethanol administration decreased weekly but not daily cumulative food intake in unrestrained rats. Food intakes of ethanol-treated and control restrained rats were comparable following 1st-3rd daily restraints, but were smaller in ethanol-treated rats following the 4th and 5th daily restraints. Open-field ambulatory activities monitored 24 h after the 5th daily restraint on the 6th day were comparable in control restrained and unrestrained rats. Ethanol-treated and control unrestrained rats also exhibited comparable ambulation, but ethanol-treated rats exhibited smaller activity than control restrained or ethanol-treated unrestrained rats. Fluid intakes of ethanol and control rats were comparable during the 2 weeks of ethanol administration, but daily restraint schedule decreased ethanol intake. The findings show adaptation to repeated restraint in control rats and inability of ethanol-treated rats to adapt in the stress schedule. These findings imply that excessive alcohol consumption may impair adaptation to stress and thus conceivably precipitate depression.

Alcoholism↗
PubMed2003

Similar effects of cocaine and immobilization stress on the levels of heat-shock proteins and stress-activated protein kinases in the rat hippocampus, and on swimming behaviors: the contribution of dopamine and benzodiazepine receptors.

Cocaine (COC) has been reported to cause effects similar to physiological stressors in the brain neuroendocrinal system, including heat-shock protein (HSP) expression, although these effects have not been elucidated in detail. In the present study, we examined the effects of repeated (4 days) treatments with cocaine hydrochloride (35 mg/kg, i.p.) and 10 min immobilization stress (IM) on the distribution of HSP (HSP27, HSP60, HSP70, HSC70) and stress-activated protein kinase (SAPK) (SAPKalpha, SAPKbeta, SAPKgamma) immunoreactive nerve cells (positive cells) in the rat hippocampus. The swimming behaviors of the rats in the forced swimming test were also examined. In both COC and IM groups, an early enhancement (5 h time point) of hippocampal HSP (HSP27, HSP60, HSP70, HSC70) and SAPK (SAPKbeta, SAPKgamma) positive cells was observed, whereas a recovery (SAPKs) or attenuation (HSP60 and HSC70) was observed at the 24 h time point. In both groups, a depression of the swimming behaviors (attenuation in the activity counts and time until immobility) below the control level was observed at the 5 h point, but a recovery was observed at the 24 h time point. At the 48 h time point, all parameters returned to the control level. These alterations in the levels of HSPs and SAPKs, and the swimming behaviors were similar to those observed in the stress (IM) group, and were characteristic in that all of these alterations were attenuated by the benzodiazepine inverse agonist, Ro 15-4513 (5 mg/kg, i.p.), and the dopamine D1 receptor antagonist, SCH23390 (0.5 mg/kg, i.p.), which was not observed in the groups treated with another stressor-like drug (bicuculline).

Animals↗
PubMed1992

The role of "stress" in workers' compensation stress claims.

Twenty-six cases of workers' compensation "stress" claims were examined to delineate the nature and causes of psychiatric illness in relation to psychological stress in the workplace. Correlations were made among demographic data, psychiatric diagnoses, categories of complaints, psychological themes, and role of litigation. Findings showed that most subjects sorted into two major groups. For the largest group (56%), symptoms were precipitated by interpersonal issues. Within this group, 86% of subjects believed that unfair treatment caused their symptoms, 79% fulfilled criteria for a personality disorder diagnosis, and 71% were more focused on issues related to their claim than on symptom relief. A smaller group (24%) encountered significant stress at work. This group had more severe psychiatric illness and fewer personality disorders.

Adult↗
PubMed2005

Conservation and divergence of circadian clock operation in a stress-inducible Crassulacean acid metabolism species reveals clock compensation against stress.

One of the best-characterized physiological rhythms in plants is the circadian rhythm of CO(2) metabolism in Crassulacean acid metabolism (CAM) plants, which is the focus here. The central components of the plant circadian clock have been studied in detail only in Arabidopsis (Arabidopsis thaliana). Full-length cDNAs have been obtained encoding orthologs of CIRCADIAN CLOCK-ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION1 (TOC1), EARLY FLOWERING4 (ELF4), ZEITLUPE (ZTL), FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1), EARLY FLOWERING3 (ELF3), and a partial cDNA encoding GIGANTEA in the model stress-inducible CAM plant, Mesembryanthemum crystallinum (Common Ice Plant). TOC1 and LHY/CCA1 are under reciprocal circadian control in a manner similar to their regulation in Arabidopsis. ELF4, FKF1, ZTL, GIGANTEA, and ELF3 are under circadian control in C(3) and CAM leaves. ELF4 transcripts peak in the evening and are unaffected by CAM induction. FKF1 shows an abrupt transcript peak 3 h before subjective dusk. ELF3 transcripts appear in the evening, consistent with their role in gating light input to the circadian clock. Intriguingly, ZTL transcripts do not oscillate in Arabidopsis, but do in M. crystallinum. The transcript abundance of the clock-associated genes in M. crystallinum is largely unaffected by development and salt stress, revealing compensation of the central circadian clock against development and abiotic stress in addition to the well-known temperature compensation. Importantly, the clock in M. crystallinum is very similar to that in Arabidopsis, indicating that such a clock could control CAM without requiring additional components of the central oscillator or a novel CAM oscillator.

Circadian Rhythm↗
PubMed2004

Vasodilator stress induces infrequent wall thickening abnormalities compared to perfusion defects in mild-to-moderate coronary artery disease: implications for the choice of imaging modality with vasodilator stress.

BACKGROUND: Experimental evidence suggests that although vasodilator stress agents consistently induce regional flow disparity between stenosed and normal coronary vascular beds, the occurrence of functional myocardial ischemia is infrequent, especially in mild-to-moderate coronary artery stenosis. Thus, it is hypothesized that dipyridamole infusion, even at high doses, will result in a disproportionately higher frequency of perfusion defects compared to regional wall thickening abnormalities. METHODS: We performed simultaneous high-dose (0.84 mg/kg) dipyridamole stress echocardiography (Echo) and Tc-99m sestamibi SPECT (MIBI, methoxyisobutyl isonitrile) in 46 patients with coronary artery diameter stenosis >50% and < o =90% in one or two epicardial coronary arteries, and no previous myocardial infarction. RESULTS: Of a total of 828 segments, MIBI showed 97 reversible defects while Echo showed only 23 reversible wall thickening abnormalities. Of the 97 segments with reversible MIBI defects, only 13 (13%) showed simultaneous reversible wall thickening abnormalities during dipyridamole infusion. There were 24 patients with MIBI defects, of whom 10 (41%) showed a corresponding wall thickening abnormality. The sensitivity of MIBI and Echo for the detection of coronary artery disease was 52% and 21%, respectively (P = 0.001). CONCLUSION: This suggests that vasodilator stress is not optimally suited for use with techniques that use regional wall thickening abnormality as a marker of ischemia for the diagnosis of coronary artery disease.

Aged↗
PubMed2005

Stress and the periodontal diseases: growth responses of periodontal bacteria to Escherichia coli stress-associated autoinducer and exogenous Fe.

Psychological stress is known to increase the circulating levels of the catecholamine hormones noradrenaline and adrenaline, which have been shown to influence the growth of a large number of bacterial species by acting in a siderophore-like manner or by inducing the production of novel autoinducers of growth. As we have previously demonstrated that periodontal organisms display differing growth responses to noradrenaline and adrenaline, the aim of this study was to determine whether these growth effects were based upon either siderophore-like or autoinducer mechanisms. Initial inocula of 43 microbial organisms normally found within the subgingival biofilm were established under anaerobic conditions (35 degrees C). Each strain was re-inoculated into a serum-based minimal medium and growth was assessed by optical density (OD(600 nm)) with test and control cultures performed in triplicate. Test cultures were supplemented with either 50 mum ferric nitrate or a previously described Escherichia coli autoinducer of growth. Significant growth effects for supplementation with ferric nitrate (13 species responding positively) and E. coli autoinducer (24 species responding positively) were observed, with differences in growth response within bacterial species and within microbial complexes. When data for all organisms were compared with published responses to catecholamines there were only weak correlations with Fe (r = 0.28) and E. coli autoinducer (r = 0.34) responses. However, large positive responses (> 25% increase) to free Fe and/or E. coli autoinducer were significantly more prevalent in the group of organisms (n = 12) known to exhibit similar responses to catecholamine hormones (P < 0.01; chi2 = 4.56). The results support the view that catecholamines may exert their effects on subgingival organisms by initiating autoinducer production, or simply by acting in a siderophore-like manner, scavenging bound iron from the local environment. It is possible that autoinducer mechanisms may play an important role in the response of oral microorganisms to stress hormones, thereby contributing to the clinical course of stress-associated periodontal diseases.

Bacteria↗
PubMed1992

Urethral stress relaxation phenomenon in healthy and stress incontinent women.

Urethral stress relaxation was induced by sudden forced dilatation. The stress relaxation parameter, Pt0.5, was defined as the relative pressure decrease 0.5 s after dilatation, where the pressures at 0.1 s and at the new equilibrium were set to 100% and 0% respectively. Pt0.5 was calculated in 28 healthy and 30 women with genuine stress incontinence. Measurements were performed at the bladder neck, in the high pressure zone and in the distal urethra. The measurements showed that all along the urethra, Pt0.5 was statistically significantly greater in the healthy women than in the incontinent women, the greatest difference being found at the bladder neck. Although there was a slight overlap in values between the 2 groups, this parameter seems useful in differentiating between competent and incompetent urethral sphincters.

Adult↗
PubMed1998

Poverty and health: exploring the links between financial stress and emotional stress in Australia.

The links between poverty and health have been investigated in a number of independent studies, as well as by the Poverty Commission in the 1970s and, more recently, the National Health Strategy. However, much of the poverty research suffers from the lack of detailed information on health status, while the work conducted in the public health sphere has used rather rudimentary poverty measures. The research reported here attempts to overcome these limitations by using unit record data from two national household surveys conducted by the Australian Bureau of Statistics in 1990. Together, these two data sets contain an enormous amount of detailed information on household incomes and the health status of individuals. Data from the two surveys are combined in a way which allows the links between poverty and health to be explored in greater detail than has hitherto been possible in Australia. Analysis of the integrated data set focuses on the links between poverty and several measures of stress-related poor health. The results from a variety of different methods point to the existence of significant differences between the reported incidence of stress of those whose incomes place them either side of a poverty threshold. The size of the statistical association between poverty and stress is of both numerical and statistical significance, although further work, preferably using longitudinal data, is needed on the important issues of causation.

Adolescent↗
PubMed1984

Modifications of the urethral rest and stress profiles after different types of surgery for urinary stress incontinence.

Full urodynamic assessment, including urethral profiles at rest and under stress, using microtransducers, was made before and at least 6 months after surgery for urinary stress incontinence in 86 patients. Cure was assessed objectively. Procedures compared were Burch colposuspension, Pereyra urethrovesical suspension and anterior colporrhaphy. The Burch colposuspension increased the pressure transmission ratio more efficiently than the vaginal operations and the cure rate was 91%. Only 50% of Pereyra operations were successful and success was related to an increase in the functional urethral length and in the pressure transmission ratio. The success rate for anterior colporrhaphy was 57% and was associated with a significant decrease in the maximal urethral closure pressure and the continence area. The prognostic value of the urethral profiles at rest and under stress and the therapeutic implications are discussed.

Adult↗
PubMed2006

A universal stress protein of Porphyromonas gingivalis is involved in stress responses and biofilm formation.

Porphyromonas gingivalis is recognized as one of the major periodontal pathogens in subgingival plaque, which is implicated in the progression of chronic periodontal disease. We analyzed the role of upsA in P. gingivalis 381 and its uspA-deficient mutant CW301 under various stress conditions. In general, the uspA mutant was less tolerant to a variety of environmental stresses relative to the parental strain. In addition, gene expression of uspA is upregulated during biofilm formation. Biofilm formation of the uspA mutant was also less than that of strain 381. In conclusion, the uspA gene affecting the stress responses of P. gingivalis is required for optimal biofilm formation.

Anti-Bacterial Agents↗
PubMed1984

Organizational and health manifestations of teacher stress: a preliminary report on the Buffalo Teacher Stress Intervention Project.

In an attempt to develop long-term stress management contingencies, four Buffalo City Elementary Schools were assessed on perceptions of organizational stress, personal manifestations, and health status. Relationships that were observed supported theoretical concepts of organizational stress, and supported the need for intervention within teacher populations. Organizational and promotional strategies to enhance participant involvement are discussed.

Adult↗
PubMed2005

Candida albicans-conditioned medium protects yeast cells from oxidative stress: a possible link between quorum sensing and oxidative stress resistance.

Candida albicans, the most frequent fungal pathogen of humans, encounters high levels of oxidants following ingestion by professional phagocytes and through contact with hydrogen peroxide-producing bacteria. In this study, we provide evidence that C. albicans is able to coordinately regulate the oxidative stress response at the global cell population level by releasing protective molecules into the surrounding medium. We demonstrate that conditioned medium, which is defined as a filter-sterilized supernatant from a C. albicans stationary-phase culture, is able to protect yeast cells from both hydrogen peroxide and superoxide anion-generating agents. Exponential-phase yeast cells preexposed to conditioned medium were able to survive levels of oxidative stress that would normally kill actively growing yeast cells. Heat treatment, digestion with proteinase K, pH adjustment, or the addition of the oxidant scavenger alpha-tocopherol did not alter the ability of conditioned medium to induce a protective response. Farnesol, a heat-stable quorum-sensing molecule (QSM) that is insensitive to proteolytic enzymes and is unaffected by pH extremes, is partly responsible for this protective response. In contrast, the QSM tyrosol did not alter the sensitivity of C. albicans cells to oxidants. Relative reverse transcription-PCR analysis indicates that Candida-conditioned growth medium induces the expression of CAT1, SOD1, SOD2, and SOD4, suggesting that protection may be mediated through the transcriptional regulation of antioxidant-encoding genes. Together, these data suggest a link between the quorum-sensing molecule farnesol and the oxidative stress response in C. albicans.

Anticoagulants↗
PubMed1990

Stress and stress counselling.

This is a report by the 1989 National Association of Clinical Tutors Wyeth Travelling Fellow to the United States of America. The stresses of postgraduate training and attempts to modify these are described, including stress counselling. The significance of stress and the relevance of the findings for postgraduate training in the United Kingdom are considered.

Counseling↗