[Plasma melatonin concentrations as additional biochemical markers for ovulation].
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The Authors report the data relative to the monitoring of menstrual cycle carried out by the observation of salivary ferning. They compared the usual physical and hormonal parameters of four groups of women with the aspect of the crystallisation of the saliva observed both by normal optical microscope and by a special type of pocket microscope which can be used by women themselves. In conclusion the Authors report a careful examination of the biochemical correlation relating the women's hormonal climate and its salivary constituents.
OBJECTIVE: To evaluate whether a urinary LH immunoassay improves timing of the postcoital test compared to traditional timing methods in normally ovulating women. METHODS: Subjects included 37 infertile couples evaluated in a tertiary care setting. A randomized, prospective trial was performed of two methods of postcoital test timing: urinary LH assay or the traditional timing by cycle-averaging and/or review of basal body temperature graphs. RESULTS: Similar serum estradiol, progesterone, LH, number of motile sperm per high-power field, cervical mucus scores, and postcoital test scores were identified in both groups. CONCLUSION: Urinary LH immunoassays do not appear to improve timing of the postcoital test as compared with traditional timing methods.
Overt (length of menstrual cycle, duration of menses, and amount of menses) and covert (luteal surge indicative of ovulation and length of luteal phase) menstrual cycle characteristics were studied in a sample of 146 women who participated in four levels of running intensity. Menstrual and running data were collected for 4 months or 3 cycles. There was a significant difference among the four groups in duration of menses and in the amount of menses, with nonrunners having significantly more days of menses and larger amounts of menses than the two highest intensity running groups. However, when a history of skipping menstrual periods between menarche and age 18 was controlled, the differences between the groups were not significant. There were no significant differences among the four groups in incidence of luteal surge or length of luteal phase. When runners were compared to nonrunners, runners reported, on average, a shorter luteal phase than nonrunners. This difference remained when controlling for age and history of skipping menstrual periods. There was no significant difference between the four groups on incidence of ovulatory disturbances (anovulation or more than one cycle with a shortened luteal phase). But, when compared to nonrunners, runners were found to have significantly more ovulatory disturbances. The best predictors of the variance in menstrual cycle characteristics were history of skipping periods between menarche and age 18, and running versus nonrunning.
Infertility is a medico social problem in Nigeria and it accounts for 40 percent of our outpatient gynaecological consultations; therefore the need to evaluate the two common presumptive methods of ovulation (serum progesterone and endometrial history) is indicated. An endometrial biopsy and three blood samples taken between M-4 and M-11 (4 and 11 days to the next menstruation) for progesterone determination in the luteal phase of the cycles of 50 infertile Nigerian women were analyzed. The methods were compared for confirmation of ovulation and evaluation of luteal functions. Total luteal phase progesterone value of 11.3 nmol/1 or greater was consistent with ovulation (secretory endometrium) in 90% of the cycles. 41 cycles yielded sufficient information to compare the two methods for evaluation of luteal function. The total progesterone value of 15 nmol/1 or greater was consistent with in-phase biopsies. Twenty eight (68.3%) of the cycles showed evidence of In-Phase (IP,) biopsies while thirteen (32.7%) showed out of phase (00P) biopsies suggestive of abnormal luteal function. The mean progesterone levels of patients with IP biopsies were significantly higher than those with 00P biopsies (P < 0.0001). Compared to studies by other workers using single midluteal progesterone assay, it seems multiple progesterone assay does not confer added advantage in confirming ovulation or in evaluation of luteal function. It is suggested that simultaneous measurements of serum progesterone levels with endometrial biopsies should be used to document ovulation and luteal function in infertile Nigerian women in view of the scarcity of ultrasonographic machines and emerging new technologies in our practice.
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The pregnancy rate is lower in mares inseminated at the first post-partum (p.p.) oestrus (40-50%) compared with pregnancy rates in subsequent oestrous cycles (55-65%). The causes of the lowered pregnancy rate are not fully understood. The aim of the present study was to examine if embryonic defects could be one of the reasons for lowered pregnancy rate. A total of 23 p.p. and 14 non-lactating control mares were flushed 7 days after detection of ovulation. Embryo recovery rate was 48% and 71% in p.p. and control mares, respectively (p = 0.16). Embryos were photographed, measured, graded and stained with fluorescein diacetate to assess their viability. Thereafter embryos were bisected and stained with Hoechst 33342 to count the cell nuclei. Embryos in both groups were equally viable and the cell numbers were not significantly different. According to morphological evaluation all embryos were classified as excellent or good. Embryos aged 7.3 to 7.6 days (+/-0.25 days) were smaller in the p.p. group than in the control group (p < 0.05). Forty-seven (9/19) and 8% (1/13) of the uterine swabs, taken before the first insemination, yielded bacteria and neutrophils in p.p. and control mares, respectively. The amount of neutrophils and/or bacteria had no statistically significant effect on embryo recovery rate (p > 0.10). Recovery of embryos was not related to histological findings in uterine biopsies taken after embryo recovery. Embryo recovery rate in p.p. mares (48%) was similar to previously reported foal heat pregnancy rates (40-50%). Hence, early embryonic death in utero would not be the most likely reason for lowered pregnancy rate in mares inseminated at the first p.p. oestrus. Sperm transport and oviductal conditions by the time of the first p.p. oestrus would need to be studied to clarify the role of fertilisation failure as the cause of lower pregnancy rate in mares inseminated at foal heat.
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A 2-year study of 135 women using the Billings Ovulation Method as their method of family planning is reported. There were 1381 exposure cycles during the 1st year and 580 during the 2nd year. The total conception rates were 1.303 for the 1st year and 1.896 for the 2nd year. If one subtract the user failures from these rates, the biologic failure rates are 0.072 for the 1st year and 0.517 for the 2nd year. The continuation rate is 51.8%. An analysis of satisfaction levels is presented with a discussion of possible underlying emotional factors.
The variation of the basophil cells number during the menstrual cycle was studied by the method of Shelley and Parnes, slightly modified. The study comprised 29 women diagnosed as primary or secondary amenorrhoea (first group) and 38 women with normal menstrual cycle, twenty of them being fertile and 18 diagnosed as secodary sterility (second group), including also two women in the second month of pregnancy. The radioimmunological measurement of luteinizing (LH) and follicle-stimulating (FSH) hormone was applied for the second group. The data obtained on 34 women of the second group showed the precipitous decrease of the number of basophils from the level of 20--50/mm3 in the follicular phase, or even their disappearance. This decrease corresponded to the LH increase. Moreover, the morphologic examination of the basophils during the menstrual cycle allowed to classify these cells into three categories: intact, partially degranulated, and firing, the number of the latter type increasing on the day of ovulation at the expense of the first two types.
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BACKGROUND: Despite some findings to the contrary, it would appear that pharmacological doses of progesterone and estrogen (alone or in combination) can influence intraocular pressure (IOP). The relationship between hormonal changes associated with the menstrual cycle and intraocular pressure is not clearly understood. After elimination of those factors that can affect IOP, the present study investigated whether physiological hormonal changes associated with the menstrual cycle have a correlation with intraocular pressure. METHODS: Intraocular pressure and the concentrations of circulating hormones, namely, the luteinizing hormone (LH), follicle-stimulating hormone (FSH), estrone (E1), estradiol (E2), progesterone, and testosterone, of twenty married women of the same age groups were recorded daily throughout a menstrual cycle. None was taking any contraceptives in any form. The intraocular pressure was measured using a Goldmann applanation tonometer. RESULTS: The intraocular pressure values fluctuated in each of the twenty subjects at various times of the cycle; however, they were not definitely correlated with the different phases of the cycle. This study failed to find any correlation between IOP and progesterone or estradiol levels.