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Fungicidal activity of essential oils of Cinnamomum zeylanicum (L.) and Syzygium aromaticum (L.) Merr et L.M. Perry against crown rot and anthracnose pathogens isolated from banana.

AIMS: To develop a post-harvest treatment system against post-harvest fungal pathogens of banana using natural products. METHODS AND RESULTS: Colletotrichum musae was isolated and identified as the causative agent responsible for anthracnose peel blemishes while three fungi, namely Lasiodiplodia theobromae, C. musae and Fusarium proliferatum, were identified as causative agents responsible for crown rot. During the liquid bioassay, cinnamon [Cinnamomum zeylanicum (L.)] leaf, bark and clove [Syzygium aromaticum (L.)] oils were tested against the anthracnose and crown rot pathogens. The test oils were fungistatic and fungicidal against the test pathogens within a range of 0.03-0.11% (v/v). CONCLUSIONS: Cinnamon and clove essential oils could be used as antifungal agents to manage post harvest fungal diseases of banana. SIGNIFICANCE AND IMPACT OF THE STUDY: Cinnamon and clove essential oil could be used as alternative post-harvest treatments on banana. Banana treated with essential oil is chemically safe and acceptable to consumers. Benomyl (Benlate), which is currently used to manage fungal pathogens, can cause adverse health effects and could be replaced with volatile essential oils.

Antifungal Agents↗

Effects of asarone and beta-asarone on conditioned responses, fighting behaviour and convulsions.

In the Ayurvedic system of medicine, the roots and rhizomes of an indigenous Indian plant Acorus calamus are used together with the roots of Rauwolfia serpentina for treating many mental ailments. The influence of asarone and beta-asarone (the trans and cis forms of 2,4,5-trimethoxy-1-propenyl benzene), two active principles of Acorus calamus, when given alone and together with either reserpine or chlorpromazine, has been studied on the conditioned avoidance response of trained rats, on the fighting behaviour of paired mice subjected to mild foot shock and on electro-convulsions. Except for electro-convulsions, asarone in small doses potentiates the effects of reserpine and of chlorpromazine; beta-asarone has no such effect. Estimation of the 5-hydroxytryptamine content of rat brain showed that neither acorus oil nor its active principles increase the concentration of 5-hydroxytryptamine; nor do these compounds cause an additional decrease in the 5-hydroxytryptamine content of the brains of animals treated with reserpine. It is concluded that the potentiating effect of these principles is unrelated to 5-hydroxytryptamine concentration. In experiments using electro-convulsions, asarone increased the percentage mortality of animals treated with chlorpromazine but not of those treated with reserpine.

Acorus↗

Modification of volatile compound profile of virgin olive oil due to hot-water treatment of olive fruit.

The effect of hot-water treatments of olive fruits before processing on the biosynthesis of virgin olive oil aroma was investigated by quantifying the variation within the major classes of volatile compounds. Data showed that hot-water treatments gave rise to changes in the volatile aroma profile of virgin olive oil from the three olive cultivars under study, Manzanilla, Picual, and Verdial. Different effects by thermal treatments were observed according to cultivar. In general, these changes are mainly due to a decrease in the contents of C(6) aldehydes and C(5) compounds. Contents of C(6) alcohols and esters remained constant or decreased slightly when the temperature of the treatment was increased. Thus, heat treatments seemed to promote a partial deactivation of the lipoxygenase/hydroperoxide lyase enzyme system, whereas other enzymatic activities, within the lipoxygenase pathway, such as alcohol dehydrogenase and alcohol acyltransferase, remained apparently unaffected as a consequence of heat treatments.

Alcohols↗

Volatile constituents in the liverwort Tritomaria polita.

The essential oil of the liverwort Tritomaria polita, collected in Otztal/Tyrol (Austria), was investigated by chromatographic and spectroscopic methods. Several new compounds were isolated by preparative gas chromatography (GC) and their structures investigated by mass spectrometry (MS) and NMR techniques. In addition to known constituents, the sesquiterpenoids (+)-eudesma-3,11-dien-8-one, (+)-eudesma-3,7(11)-dien-8-one, (+)-6,11-epoxy-eudesmane, (-)-6,7-seco-eudesm-7(11)-en-6-al, (+)-6beta-hydroxy-eudesm-11-ene, (-)-6alpha-hydroxy-eudesm-11-ene, (+)-6,11-epoxy-isodaucane could be identified as natural compounds for the first time.

Gas Chromatography-Mass Spectrometry↗

Determination of volatile organic contaminants in bulk oils (edible, injectable, and other internal medicinal) by purge-and-trap gas chromatography/mass spectrometry.

Purge-and-trap gas chromatography/mass spectrometry is evaluated for the quantitation of part-per-billion levels of volatile organic contaminants in bulk vegetable oils. Results using 2 purge techniques (direct purging of the heated oil and purging after dispersing the oil on an aluminum oxide powder) and 2 quantitative methods (standard curve and deuterium-labeled internal standard addition) are reported. Twenty volatile compounds and 8 vegetable oils were investigated. Recovery data and estimated detection limits for each compound are reported for each purge technique. Generally acceptable recoveries (70-130% for more than 90% of the analyte spikes) and acceptable detection levels (approximately 4-10 ppb) were obtained for all compounds using either the external standard curve or the deuterium-isotope-labeled internal standard. The use of a dispersant (such as alumina) for sample purging resulted in poor recoveries of the highly volatile contaminants.

Aluminum Oxide↗