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PubMed1988

Subcellular fractionation of the porcine corpus luteum: sequestration of progesterone in a unique particulate fraction.

Homogenates of porcine corpus luteum were subjected to fractionation by differential-rate centrifugation or sucrose density gradient fractionation, with or without pretreatment with digitonin. Fractions of each gradient were assayed for a number of markers characteristic of the major intracellular organelles and cell-surface membranes, and for progesterone content. The majority of the progesterone content of homogenates of porcine corpus luteum was associated with a low-density particulate fraction which equilibrated at a buoyant density of 1.07-1.09 g/cm3. Pretreatment with digitonin increased the buoyant density of the progesterone-enriched fraction markedly (to 1.13-1.15 g/cm3) without causing release of steroid. The density distributions of progesterone content in control and digitonin-treated luteal gradient fractions were quite distinct from those of the major intracellular organelles and luteal cell-surface membranes. However, NADH-cytochrome C reductase activity (but not other endoplasmic reticulum markers) was also enriched in this fraction. The results suggest that most of the progesterone of the porcine corpus luteum is associated with a unique particulate fraction which is enriched in digitonin-reactive lipids and NADH-cytochrome C reductase activity.

Animals↗
PubMedDate not supplied

[Protein content and activity of peptide hydrolases in subcellular fractions of the hypothalamus at various levels of feeding motivation].

After 3-days fasting the specific activity of neutral peptide-hydrolases decreased in the homogenate and nuclear fractions and increased in the mitochondrial and soluble ones. After 5-7-days fasting the above peculiarity persisted. Under the diet recovery specific enzyme activity was recovered up to the control level on the 30th day only. The obtained data prove an intensive renewal of the neutral and acid proteins in the subcellular hypothalamic structures depending on different levels of feeding motivation. This increased functional activity of proteinases is aimed at providing for informational and intracellular processes comprising energetic and structural plasticity of the neuronal organizations of the hypothalamic functional feeding system.

Animals↗