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Effects of ozone and simulated acid rain on birch seedling growth and formation of ectomycorrhizae.

Four-week-old paper birch (Betula papyrifera Marsh.) seedlings, inoculated or non-inoculated with the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker & Couch and grown in steamed or non-steamed soil, were exposed to ozone (O(3)) and/or simulated acid rain (SAR). Plants were exposed to O(3) for 7 h per day on 5 days per week for 12 weeks. O(3) concentrations were maintained between 0.06 and 0.08 ppm. SAR was applied 10 min per day on 2 days per week. O(3), SAR, soil regime and mycorrhizal treatment did not significantly affect any of the measured variables. Interactions between O(3) and SAR, SAR and mycorrhizal treatment, soil regime and mycorrhizal treatment and ozone and soil regime had significant effects. Treatment of seedlings with pH 3.5 SAR caused increases in growth which were more apparent in birch exposed to O(3). Mucorrhizal treatment caused increased growth in non-steamed soil, while growth appeared to decrease in steamed soil. Birch seedlings grew much better in steamed soil. The implications of increased growth in steamed soil may demonstrate the importance of looking at the secondary effects of pollutants on soil-borne organisms.

Journal Article↗

Improvement of thermal reactivation of activated carbon for the removal of 2-methylisoborneol.

Rapid small-scale column tests (RSSCTs) were employed to evaluate the impact of the dissolved oxygen (DO) concentration in the water used to create steam on the reactivation of spent granular activated carbon (GAC) using three novel thermal reactivation procedures: steam-curing, steam-curing with ramped temperature, and steam pyrolysis reactivation. Evaluation of the physical properties of the reactivated carbon showed a change in mass and volume loss with a change in DO. Performance testing of the carbons for removal of the taste- and odor-causing compound 2-methylisoborneol (MIB) showed that MIB uptake generally increased as the DO concentration decreased. Decrease in MIB removal with an increase in surface acidity, a phenomenon found in the literature, may be responsible for the changes in adsorption performance, as the higher DO concentrations yielded carbons with higher total surface acidity. In addition, the steam-curing process, which was implemented at 375 degrees C (i.e. about 400 degrees C lower than typical reactivation temperatures) with a low DO concentration (i.e. 3-4mg/L) had comparable performance to the virgin carbon counterpart, which could manifest cost-savings due to the low temperature associated with this protocol compared to conventional reactivation. Furthermore, since the mass loss associated with this steam-curing protocol was low, less virgin carbon make-up would be required also improving the economic viability of this reactivation protocol.

Adsorption↗

Influence of holding temperature on the growth and survival of Salmonella spp. and Staphylococcus aureus and the production of staphylococcal enterotoxin in egg products.

In this study, growth and survival of Salmonella spp. and Staphylococcus aureus in steamed egg and scrambled egg held at 5, 18, 22, 37, 55 and 60 degrees C are investigated. The production of staphylococcal enterotoxin in steamed egg is also examined. Results reveal that Salmonella spp. and Staph. aureus in the egg products multiply best at 37 degrees C, followed closely by 22 and 18 degrees C. Neither pathogen showed growth in the egg products held at 5 degrees C. Initial inoculation dose, holding temperature and holding time affected the population of both organisms found in the egg products. Staphylococcal enterotoxin A (SEA) and B (SEB) are detected only in the egg products held at 37 or 22 degrees C. After holding at 37 degrees C for 36 h, scrambled egg inoculated with ca. 5.0 log cfu/g Staph. aureus contains the highest levels of SEA (> 64 ng/g) and SEB (> 64 ng/g). Although Salmonella spp. and Staph. aureus grow better in steamed eggs than in scrambled eggs, production of staphylococcal enterotoxin, in general, was higher in scrambled eggs than in steamed eggs. On the other hand, a repaid destruction of the test organisms in steamed eggs held at 60 degrees C was observed. Holding the steamed eggs at 60 degrees C, Salmonella spp. and Staph. aureus with an initial population of ca. 5.9 and 5.6 log cfu/g, respectively, reduced to a non-detectable level in 1 h.

Colony Count, Microbial↗

Evaluation of beta-lactose, PVP K12 and PVP K90 as excipients to prepare piroxicam granules using two wet granulation techniques.

The present investigation aimed at evaluating the use of different excipients, beta-lactose and polyvinylpyrrolidone of two molecular weights (PVP K12 and PVP K90), in the production of improved release piroxicam granules, by wet granulation using both water and steam as granulation liquid. The formulations examined were: piroxicam (Px)/beta-lactose; Px/PVP K12 and Px/PVP K90, each one at a 1:9 weight ratio. The most significant difference between beta-lactose and PVP is that, using the first excipient, both steam and water granules were produced while, when PVP were employed, only steam granules were obtained. Image analysis revealed that beta-lactose steam granules had a larger surface area with respect to water granules, whereas lower values of this parameter were observed in PVP-s granules, confirming the Scanning Electron Microscopy micrographs and the fractal analysis results. As regards the enhancement of the dissolution profiles, the best result was obtained using beta-lactose steam granules followed by PVP K12 ones, even if the reactive dimension values indicated that during the dissolution process PVP K12 granules modified the surface more than beta-lactose granules. As regards PVP K90, this excipient was the one less influencing the granule morphology and the dissolution behaviour. Differential Scanning Calorimetry analysis suggested the partial amorphisation of the drug in the granules containing the three excipients. This result was then confirmed by X-ray powder diffraction analysis. Therefore, beta-lactose and PVP K12 could be proposed as useful excipients to enhance the dissolution rate of Px from granules prepared using the steam granulation technique.

Drug Evaluation, Preclinical↗

Modeling transport processes in sterilization-in-place.

SIP (sterilization-in-place) of equipment using saturated steam is limited by transport processes that restrict the distribution of sterilizing steam. The following are two crucial operations: the removal of air prior to sterilization, and the removal of condensate during the sterilization. Using simple model systems of pipes and tanks, characteristic operating parameters were examined and steady-state models were analyzed. The results were used to evaluate design aspects of SIP, including heat insulation, spacing of steam traps, sloping of lines, steam velocities and consumption, placement of temperature sensors, and scale factors in piping. A more reliable SIP design is achievable by insulating equipment, spacing steam traps to limit condensate buildup, providing an effective air removal operation, and providing reliable, high-quality steam.

Hot Temperature↗

The incidence of swine dysentery in pigs can be reduced by feeding diets that limit the amount of fermentable substrate entering the large intestine.

Two experiments were conducted to test the hypothesis that feeding diets which limit the amount of fermentable substrate entering the large intestine would protect pigs against experimental infection with Serpulina hyodysenteriae, the causative agent of swine dysentery. Experiment 1 examined the effect of grain processing (hammer milling vs. steam flaking) and grain type (barley, groats, corn, sorghum and wheat) on indices of fermentation in the large intestine and the incidence of swine dysentery. Experiment 2 examined the role of five diets, steam-flaked corn, steam-flaked sorghum, hammer-milled wheat, extruded wheat and cooked white rice, on these same measures. All diets contained an animal protein supplement and no antibiotics. Pigs fed diets based on steam-flaked corn and steam-flaked sorghum had a lower incidence of disease (11-33%) than pigs fed diets based on other grains (75-100%). Pigs fed the diet based on cooked white rice were fully protected against swine dysentery. Both the soluble non-starch polysaccharide (NSP) concentration and the total NSP concentration of the diets explained a significant proportion of the variation in swine dysentery (R2 = 0.56, P = 0.016, and R2 = 0.71, P = 0.002, respectively), such that pigs eating diets containing <1.0 g/100 g soluble NSP showed reduced disease. However, pigs fed corn, sorghum and steam-flaked sorghum (Experiment 2), which contained only 0.4-0.5 g/100 g soluble NSP, still had a high incidence of disease (>50%). This was attributable to a higher level of resistant starch present in these grains. These data provide evidence that the expression of swine dysentery is associated with an increased concentration of fermentable substrate entering the large intestine.

Adenosine Triphosphate↗

The effect of grain source and grain processing on performance of feedlot cattle: a review.

Effects of grain species and grain processing method on DMI, rate and efficiency of gain, and feeding value for cattle fed high concentrate diets were appraised by statistically compiling results from 605 comparisons from feeding trials published in North American journals and experiment station bulletins since 1974. Metabolizable energy (ME) values for each grain and processing method were calculated by quadratic procedures from DMI and animal performance. Averaged across processing methods, ME values for corn, milo, and wheat grain (3.40, 3.22, and 3.46 Mcal/kg DM) fell within 9% of ME estimates from NRC (1996) for beef cattle. In contrast, ME values for barley and oats grain (3.55 and 3.46 Mcal/kg DM) were 24% and 17% greater than NRC (1996) estimates. Compared with the dry rolled forms, high moisture corn and milo resulted in lower ADG and DMI. Compared with dry rolling, either steam rolling or flaking of corn, milo, and wheat decreased DMI without decreasing ADG and improved feed efficiency by 10, 15, and 10%, respectively. Compared with dry rolled grain, steam flaking increased (P < .05) body weight-adjusted ME of corn and milo grain by 15 and 21%, respectively; body weight-adjusted ME for whole corn was 9% greater (P < .05) than for rolled corn grain. Steam flaking was surprisingly effective (13%) at increasing (P < .05) the body weight-adjusted ME of wheat, but steam flaking failed to increase the ME of barley and oats. Higher moisture content of high-moisture corn decreased dry matter intake without depressing ADG and improved efficiency and increased ME of the grain. Compared with steam flakes of moderate thinness, processing milo or barley to a very thin flake tended to reduce ADG and failed to improve feed efficiency. The ideal roughage source and roughage moisture content for maximum ME and ADG varied with grain processing method. Feeding corn silage rather than alfalfa and wet rather than dry roughage depressed (P < .01) ADG of cattle and reduced (P < .01) body weight-adjusted ME of cattle fed high-moisture corn grain but tended to increase both with steam-flaked corn or wheat.

Animals↗

Comparison of barley and sorghum grain processed at different densities for lactating dairy cows.

To vary ruminally degradable starch, sorghum grain was dry-rolled or steam-flaked to different densities and compared with dry-rolled barley in total mixed diets fed to 40 lactating cows (111 d of lactation) assigned to five dietary treatments. Diets contained (percentage of dry matter) 35% alfalfa hay, 4.1% cottonseed hulls, 10% whole cottonseed, 2% fish meal, 4% soybean meal, and 4.9% of a molasses, mineral, and vitamin supplement. Treatments were 40% sorghum grain either dry-rolled or steam-processed at flake densities of 437, 360, and 283 g/L. A fifth diet containing 42% dry-rolled barley was fed. Cows were blocked according to pretreatment (14 d) milk yield and received experimental diets for 56 d. Increasing ruminal starch degradability by including steam-flaked sorghum grain or barley in the diet did not increase milk yield or milk protein percentage and yield, as was shown in eight previous studies. Steam-flaked sorghum or dry-rolled barley in the diet decreased dry matter intake, resulting in a 10 to 19% higher efficiency of conversion of feed dry matter to milk than that for dry-rolled sorghum. Milk urea N was decreased, and milk casein yield tended to be increased, by steam-flaking sorghum at the moderate density compared with dry-rolling or fine flaking. Dietary protein was more efficiently converted to milk protein and casein from flaked sorghum and dry-rolled barley than from dry-rolled sorghum. In this study, increasing ruminal starch degradability resulted in higher feed efficiency and lower feed intakes, bu optimal flake densities for steam-processed sorghum gain to maximize milk and milk protein yield were not clarified.

Animal Feed↗

Improving energy supply to late gestation and early postpartum dairy cows.

Sixty-five multiparous Holstein cows were used to test the effects of feeding diets of varied ruminal carbohydrate availability during the transition period on dry matter intake, blood metabolites, and lactational performance. Cows received total mixed rations containing either cracked corn or steam-flaked corn beginning 28 d prior to expected calving date. At parturition, cows were assigned to a postpartum total mixed ration that contained either cracked corn or steam-flacked corn. Diets were fed until 63 d in milk. No treatment effects on prepartum or postpartum dry matter intake, body weight, and body condition score were observed. Cows fed steam-flaked corn had lower blood urea N concentrations during the prepartum period and lower plasma nonesterified fatty acid concentrations during the prepartum and postpartum periods. Cows fed steam-flaked corn postpartum produced 2.3 kg/d more milk than cows fed cracked corn during the first 63 d in milk. Fat corrected milk showed no treatment effect. Seven cows were used to evaluate treatment effects on ruminal fermentation and digesta kinetics. Prepartum and postpartum treatments had minimal effects on ruminal fermentation. Feeding steam-flaked corn prepartum decreased apparent fiber digestibility and ruminal NH3 N. Feeding steam-flaked corn postpartum decreased the acetate to propionate ratio. Prepartum and postpartum treatments did not affect digesta kinetics. An increase in ruminal carbohydrate availability during the postpartum period enhanced milk production, but had variable results on ruminal fermentation.

Animal Feed↗

Effect of heat treatment on hepatitis A virus and norovirus in New Zealand greenshell mussels (Perna canaliculus) by quantitative real-time reverse transcription PCR and cell culture.

Quantitative real-time reverse transcription PCR (qRT-PCR) and cell culture (50% tissue culture infectious dose [TCID50]) were used to determine the effect of heat treatments on norovirus and hepatitis A virus (HAV) in the New Zealand Greenshell mussel (Perna canaliculus). Since it is common practice to cook mussels until the shells open, internal temperatures and opening times of mussels on boiling and steaming were determined at regular time intervals. Fifty mussels in batches of six were exposed to boiling and steaming. A mean internal temperature of 90 degrees C (recommended for virus inactivation when maintained for 90 s) was reached after boiling for 170 s, with all 50 mussels open at 210 s. For steaming, the mean internal temperature achieved was only 83 degrees C after 300 s, and all 50 mussels were open. When mussels were steamed for 180 s (mean internal temperature of 63 degrees C), a significant 1.5-log decrease in the HAV titer (log TCID50) was observed. Following the immersion of mussels in boiling water for 180 s (mean internal temperature of 92 degrees C), no viable HAV was detected. For both boiling and steaming experiments, there was no significant change in the norovirus or HAV qRT-PCR titers compared with the controls. Our results show that when New Zealand Greenshell mussels open on heating, their internal temperature may not reach the parameters required for virus inactivation. Immersion for a minimum of 3 min in boiling water rather than steaming is recommended to reduce the risk of viral foodborne illness from contaminated shellfish.

Bivalvia↗

Predicting color kinetics during red Asian ginseng (Panax ginseng) preparation.

A red Asian ginseng preparation was prepared as follows: fresh Asian ginseng (Panax ginseng) roots were first steamed for half an hour to several hours, and then the steamed roots were dried into died roots (red Asian ginseng, 10% moisture content, on a dry basis). During steaming, the color of ginseng (white) turned yellow and brown during subsequent steaming. Color is an important index for grading red Asian ginseng. In this study, the effects of drying time and temperature on the surface color formation (L, a/b) of the Asian ginseng, and the color formation (L, a/b) of the red Asian ginseng powder were investigated. The value of L decreased, while the value of a/b increased as drying proceeded; the value of L was slightly influenced by the drying temperature, but the value of a/b was markedly influenced. With respect to the color of the final product (red ginseng), the value of L was slightly influenced by steaming time and temperature, while the value of a/b was increased as steaming time and drying temperature increased. Based on the nth-order rate equation and Arrhenius equation, a kinetic model for describing these effects was established, and the results were satisfactorily fitting.

Algorithms↗

[Application of a new method for the calculation and description of the resistance of microbiological indicators. I. Testing of several common microbiological sterilization indicators (author's transl)].

The method described by SPICHER and PETERS (1975) for the calculation and description of the resistance of microbiological indicators was tested. As test objects served spore-containing earth according to DIN 58946, Attest indicators (3 M Company, Minnesota) and Oxoid Spore Strips (Oxoid Ltd., London). The tests were performed not only for different batches of indicators but also for preparations of different age. After application of steam (120 degrees C), the indicators were examined for the presence of surviving germs capable of multiplication. When plotting the frequency of indicators with surviving germs (q) against the duration of steam action, S-shaped curves were obtained as expected. By altering the scale of the ordinate (y = lg (-ln(1 - q))), the S-shaped curves could be transformed into straight lines. Thus, the experimentally established paired values could be used for a calculation of regression. This method of calculation proved to be suitable in all cases studied. By indicating the position and the slope of these straight regression lines, the resistance of microbiological indicators can be exactly described (cf. Table 2). This method is applicable not only to indicators containing culture spores but also for native spore-containing earth. The indicators examined differed in their resistance and stability. Seven out of eight batches of Attest indicators (cf Figs. 1 and 2 and Table 1) fulfilled the requirements of DIN 58946, Part 4, for the resistance of bio-indicators for steam sterilization. One of the batches had a slightly higher resistance. The Attest indicators tested were of good stability (see Fig. 1 and Table 1). Where surviving germs were present on the indicators after treatment by steam, their growth was recognizable, in 99% of cases, already after incubation of the cultures for 24 hours. Only two batches of Oxoid Spore Strips were available for testing. One batch was of a higher resistance than required by DIN 58946. The second batch was slightly above the lower limit of the permissible range (see Fig. 3). During storage for 12 months, the resistance of both batches was reduced by 3--4 min. Where the indicators exhibited surviving germs after treatment by steam, growth was recognizable in 87% of the cases after incubation for 24 hours, while for the other indicators, incubation for 48 hours was necessary. The experiments confirmed the good stability of native spore-containing earth (see Fig. 5). Within 4--5 years, the steam resistance of the preparations decreased only by 3--4 min.

Bacteriological Techniques↗

Heated, humidified air for the common cold.

OBJECTIVES: To assess the effects of inhaling heated water vapour with the help of a rhinotherm (an equipment designed to deliver heated water vapour to a person's nasal cavity), in the treatment of the common cold by comparing a. symptoms b. viral shedding c. nasal resistance after a natural or experimentally induced common cold. SEARCH STRATEGY: We searched MEDLINE with MeSH headings: common cold, rhinopharyngitis, inhalation, steam, heated vapour, rhinothermy, till July 1999. EMBASE, Current Contents, review articles, cross references were also searched. Attempts were also made to contact the manufacturers for any unpublished data. SELECTION CRITERIA: Randomized trials using heated water vapour in a standardized way in patients with the common cold or volunteers with experimental induction of rhinovirus infection were included in the review. DATA COLLECTION AND ANALYSIS: All the articles retrieved were initially subjected to a review for inclusion / exclusion criteria. Review articles, editorials, abstracts with inadequate outcome description were excluded. Studies selected for inclusion were subjected to a methodological assessment. MAIN RESULTS: The results of a systematic review of six trials with 319 participants, support the use of warm vapour inhalations in the common cold in terms of relief of symptoms (Odds Ratio with 95 % CI 0.31, 0.16-0.60, Relative risk 0.56, 0.4-0. 79). Results on symptom score indices were equivocal. None of the studies demonstrated a worsening of clinical symptom scores. One study demonstrated increased nasal resistance one week after steam inhalation in contrast to an earlier study which showed improvement in the nasal resistance. There was no evidence of decreased viral shedding measured by virus isolation in the nasal secretions (Tyrrell 1989) or measurement of viral titres in nasal washings among treatment group. The rhinovirus titres in the nasal washings from the treatment group were the same as those of the placebo group on day one prior to the treatment and on all four days after the treatment. The area under curve (Hendley 1994) was also similar in the placebo and treatment groups for titres of virus in the nasal washings as were the average viral titres across five days of follow up, the maximum values after treatment, and viral shedding velocity i.e. amount of virus shed per day (Hendley 1994). Minor side effects due to thermal stress were reported in all the studies. REVIEWER'S CONCLUSIONS: Three trials demonstrated beneficial effects on the symptoms of the common cold. One study from Israel showed a decrease in nasal resistance measured by peak nasal expiratory and inspiratory flow rate. Studies done in North America failed to show any objective improvement in outcome measures with the study intervention. A multi-centre double blind randomised controlled trial testing this therapy with uniform outcome measures is recommended.

Air↗

Adsorption of volatile organic compounds by pecan shell- and almond shell-based granular activated carbons.

The objective of this research was to determine the effectiveness of using pecan and almond shell-based granular activated carbons (GACs) in the adsorption of volatile organic compounds (VOCs) of health concern and known toxic compounds (such as bromo-dichloromethane, benzene, carbon tetrachloride, 1,1,1-trichloromethane, chloroform, and 1,1-dichloromethane) compared to the adsorption efficiency of commercially used carbons (such as Filtrasorb 200, Calgon GRC-20, and Waterlinks 206C AW) in simulated test medium. The pecan shell-based GACs were activated using steam, carbon dioxide or phosphoric acid. An almond shell-based GAC was activated with phosphoric acid. Our results indicated that steam- or carbon dioxide-activated pecan shell carbons were superior in total VOC adsorption to phosphoric acid-activated pecan shell or almond shell carbons, inferring that the method of activation selected for the preparation of activated carbons affected the adsorption of VOCs and hence are factors to be considered in any adsorption process. The steam-activated, pecan shell carbon adsorbed more total VOCs than the other experimental carbons and had an adsorption profile similar to the two coconut shell-based commercial carbons, but had greater adsorption than the coal-based commercial carbon. All the carbons studied adsorbed benzene more effectively than the other organics. Pecan shell, steam-activated and acid-activated GACs showed higher adsorption of 1,1,1-trichloroethane than the other carbons studied. Multivariate analysis was conducted to group experimental carbons and commercial carbons based on their physical, chemical, and adsorptive properties. The results of the analysis conclude that steam-activated and acid-activated pecan shell carbons clustered together with coal-based and coconut shell-based commercial carbons, thus inferring that these experimental carbons could potentially be used as alternative sources for VOC adsorption in an aqueous environment.

Adsorption↗

Singlet oxygen mediated degradation of lignin--a kinetic study.

Singlet oxygen mediated degradation was carried out on lignin from steam-exploded straw, organosolv lignin and alkali lignin. The lignins were characterized by elemental analysis, UV, FTIR, 1H and 13C NMR, gel permeation chromatography, and thioacidolysis. The kinetics of singlet oxygen degradation were obtained by following the gel permeation chromatograms in the presence of an external standard. This procedure cannot be used for alkali lignin. In the case of steam-exploded and organosolv lignins, the reactions follow zero-order kinetics for the first 4-8 hours; thereafter, the reactions slow down and follow slower zero-order kinetics This behaviour can be explained assuming that easily degradable structures in the outer sphere of lignins are degraded in the first period, while the core lignin is degraded in the second one. The degradation in organosolv lignin is faster than in steam-exploded lignin. Molecular weight distribution and the absence ofguaiacyl units in steam-exploded lignin can give rise to this behaviour. Similar behaviour was obtained in the singlet oxygen degradation of the pulp of steam-exploded straw.

Kinetics↗

Evaluation of 5 types of fishing material, 2 sterilization methods, and a crimp-clamp system for extra-articular stabilization of the canine stifle joint.

OBJECTIVE: To evaluate the mechanical properties of 5 types of fishing material, 2 sterilization methods, and a commercially designed crimp-clamp system for the extra-articular repair of the canine stifle joint. STUDY DESIGN: Experimental study. SAMPLE POPULATION: Animals were not used in this study. METHODS: Two brands of monofilament nylon fishing line and 3 brands of monofilament nylon leader line were used to determine the effect of steam and ethylene oxide sterilization on strength and elongation of the material. A strand of 36-kg test monofilament nylon fishing material was wrapped around 2 rods or knotted to form a loop around 2 rods on a materials-testing machine. Ten trials of each brand of unsterilized, steam-sterilized, and ethylene oxide-sterilized fishing material were tested. A strand of each material was elongated to failure at a constant displacement of 1,000 mm/min to determine strength. A strand of each material was cycled 10 times to a load of 50 N to determine percent elongation. The brand of fishing material with the greatest strength and least elongation was crimped to form a loop around 2 rods on a materials-testing machine and tested as described above. ANOVA was used to determine the effect of sterilization method, brand of material, knot, wrap, and crimp on strength and elongation of the material, and a post-hoc t test was used when significant differences were found. A Student t test was used to compare fixation techniques (wrap, knot, and crimp). RESULTS: Sterilization by steam or ethylene oxide had no significant effect on the strength of the nylon fishing material. Steam sterilization resulted in significant increases (2- to 4-fold) in elongation of most nylon fishing material when compared with unsterilized material. Ethylene oxide sterilization had minimal effect on elongation of the fishing material. Mason leader line showed no significant change in strength or elongation regardless of sterilization method. Significantly less strength and significantly less elongation were demonstrated in Mason leader line that was crimped as compared with Mason leader line that was knotted. CONCLUSION: Ethlylene oxide was the preferred method of sterilization to preserve strength and minimize elongation of the fishing material. Of the materials tested, Mason leader line had the least elongation and the greatest preservation of strength when ethylene oxide was used as the sterilization method. Mason leader line and Sufix fishing line were comparable choices when steam was used as the sterilization method. Significantly less elongation was demonstrated in crimped Mason leader line as compared with knotted Mason leader line. CLINICAL RELEVANCE: Of the materials tested, Mason leader line and Sufix fishing line had the best mechanical properties for extracapsular stabilization of the canine stifle joint. Crimping is an attractive alternative to knotting and results in a reduction in elongation of the nylon fishing material.

Animals↗

Ventilatory responses of normal subjects to flax dust inhalation: the protective effect of autoclaving the flax.

A homogeneous batch of dew retted hackled flax was divided into two portions. One was untreated and the other was steamed for 45 minutes at 125 degrees C in three pressure/vacuum cycles in an autoclave. Dust was collected when the two flaxes were separately processed by industrial doubler and stapler machines. From untreated flax 7.2 g of dust was collected per kilogram of flax after two processing operations. From the steamed flax 4.4 g of flax was obtained per kilogram after four operations. A method was devised to disperse the dust in a room to produce dust levels similar to those encountered in a dusty mill (4.5-5.7 mg/m3). Twelve normal volunteers from the managerial staff of the linen industry of Northern Ireland inhaled the dust over six hour periods. With the untreated flax decreases were obtained in mean forced expiratory measurements of 7.6% in FEV1 and 4.5% in FVC (p less than 0.01). A double blind crossover comparison of similar levels of untreated and steamed flax dusts showed 30% less impairment of the forced expirations with steamed than with untreated flax (p less than 0.05). If these responses reflect the long term airway effects of flax dust then the steaming of flax may help in reducing byssinosis.

Adult↗

Effectiveness of three types of sterilization on the contents of sharps containers.

The purpose of this study was to test the effect of treatment in a gravity steam autoclave, high-vacuum steam autoclave, or an unsaturated chemical vapor sterilizer on endospores present on strips or placed inside of dental anesthetic cartridges held within sharps containers. Strips with 1.7 X 10(5) Bacillus stearothermophilus endospores were used; the cartridges were soiled with an equal number of spores or spores mixed with blood. If sterilization was not accomplished after the initial period, additional exposure time was added. Neither the presence of blood or anesthetic solution nor the position of the container affected the efficiency of sterilization. Soiled cartridges were much more difficult than strips to sterilize. Intact cartridges could not be sterilized by two runs in a gravity steam autoclave or an unsaturated chemical vapor sterilizer or one run in a high-vacuum steam autoclave. Sterilization occurred after two runs in the gravity steam autoclave and unsaturated chemical vapor sterilizer only when one end of the cartridge was removed prior to processing. Results indicated that unopened spore-soiled cartridges are not readily sterilized by commonplace office sterilizers, even after extended exposure.

Anesthesia, Dental↗