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Natural contamination of oat with group A trichothecene mycotoxins in Poland.

Ninety-nine naturally contaminated oat grain samples were collected in 12 plant breeding stations in different parts of Poland. T-2 toxin, HT-2 toxin and diacetoxyscirpenol (DAS) levels were determined by gas chromatography with mass selective detection (GC-MS). HT-2 was the major toxin with an incidence of 24% and its average level in positive samples was 21 microg kg(-1). The incidence of T-2 and DAS was 15 and 12%, and their average levels were 60 and 23 microg kg(-1), respectively. The highest concentrations of HT-2, T-2 and DAS were 47, 703 and 111 microg kg(-1), respectively. Sixty-five samples were free of detectable amounts of the toxic metabolites analysed.

Avena↗

ICP/MS and ICP/AES elemental analysis (38 elements) of edible wild mushrooms growing in Poland.

Thirty-eight elements, including toxic cadmium, lead, mercury, silver and thallium, were determined in 18 species of wild edible mushrooms collected from several sites in Pomorskie Voivodeship in northern Poland in 1994. Elements were determined by double focused high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) and inductively coupled plasma atomic emission spectroscopy (ICP-AES), after wet digestion of the dried samples with concentrated nitric acid in closed PTFE vessels using a microwave oven. K, P and Mg were present at levels of mg/g dry matter; Na, Zn, Ca, Fe, Cu, Mn, Rb, Ag, Cd, Hg, Pb, Cs, Sr, Al and Si were present at microg/g levels, while Tl, In, Bi, Th, U, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, La, Lu and Ba were present at ng/g levels.

Agaricales↗

Predicted intake of trace elements and minerals via household drinking water by 6-year-old children from Kraków (Poland). Part 1: lead (year 2000).

A population of 232 children living in urban, peripheral and rural areas of southern Poland was included in the study. Household drinking water was collected twice a day, early in the morning and in the evening. The lead (Pb) concentration was estimated by a graphite furnace-atomic absorption spectroscopy (GF-AAS) method. The geometric mean of Pb concentration in drinking water in 2000 for evening samples was 0.68, 1.24 and 2.28 microg x l(-1) for urban, peripheral and rural areas, respectively, while for morning samples it was 1.42, 2.16 and 2.97 microg x l(-1). These areas differed significantly (p < 0.05) and odds ratio for peripheral versus urban areas was 1.6. The difference of Pb concentrations in the morning and evening water was significant (p < 0.001). The mean ratio was 1.8 and the median difference 0.8 microg x l(-1). The predicted contribution of drinking water to the Pb intake by 6-year-old children living in urban, peripheral and rural areas was 1.2, 2.0 and 5.6% of the provisional tolerable weekly intake (PTWI) respectively. For the 4.3-11.1% of children, the Pb concentration in water > 10 microg x l(-1) and in these cases the predicted Pb intake with drinking water contributed to PTWI by 22.3-30.0%. The simple test introduced in this study, the analysis of morning and evening water samples, proved that contamination of water in the plumbing is significant and common. The above parameters can be applied to routine quality control of drinking water performed in the households. The in-house water contamination should be considered as a factor in public health protection programmes with special attention to children.

Analysis of Variance↗

Determination of total mercury in foods of plant origin in Poland by cold vapour atomic absorption spectrometry.

Total mercury concentrations were determined in 573 samples of agricultural crops and foods of plant origin which included cereals, fruit and vegetables and their products commercially available on the Polish market. The method of cold vapour atomic absorption spectrometry (CVAAS) after a wet-acid digestion in a closed-vessels and microwave oven was used and the reliability of the procedure demonstrated. Mercury concentrations in the agricultural crops and plant foods were generally below the maximum permissible limits in Poland and rarely exceeded 5 microg x kg(-1). Values ranged from <0.1 to 14 microg x kg(-1), mean 2.4 +/- 2.3 microg x kg(-1) in wheat and rye grains; from <0.1 to 2.4 microg x kg(-1), mean 0.5 +/- 0.4 microg x kg(-1) in nine varieties of vegetables; from <0.1 to 5.1 microg x kg(-1), mean 1.1 +/- 0.9 microg x kg(-1) in seven varieties of fruit; from <0.1 to 5.6 microg x kg(-1) in cereal products and jams; and from <0.1 to 3.0 microg x l(-1) in fruit and vegetable juices, nectars and beverages. The contribution of the mercury in the analysed agricultural crops and foods of plant origin to the weekly dietary intake of total mercury was 8 microg/person, which represents only 3% of the provisional tolerable weekly intake (PTWI) for this metal.

Crops, Agricultural↗

Exposure to cadmium, lead and mercury in the adult population from Eastern Poland, 1990--2002.

The aim was to estimate the adult exposure to cadmium, lead and mercury from daily household diets in Eastern Poland (Lublin city and province). A duplicate diet approach was used to top collect diet samples in 1990, 1993, 1998 and 2002. Cadmium and lead contents were measured by flame atomic absorption and the mercury content was measured by cold vapour atomic absorption spectrometry. The intake of the three elements was calculated using FOOD computer software. The exposure to cadmium taken with daily diets was from 16.4-34.5 microg/person/day (27-58% PTWI). The lead exposure was 66.5-106 microg/person/day (31-49% PTWI), which posed a smaller risk, and the exposure risk to mercury of 4.08-6.65 microg/person/day (10-16% PTWI) was even lower.

Adult↗

Predicted intake of trace elements and minerals via household drinking water by 6-year-old children from Kraków, Poland. Part 2: Cadmium, 1997-2001.

Between 1997 and 2001, samples of drinking water (DW) were collected in about 600 households and pre-schools by a double sampling method, early in the morning (stagnant water, W1) and in the evening (flushed water, W2). The study group included about 300 5-7-year-old children living in urban (metropolitan, U) and peripheral (P) areas of Kraków and rural (R) areas in southern Poland. Cadmium concentrations were estimated by the GF-AAS method with Zeeman background correction. Mean cadmium concentrations in water did not vary substantially by year. Moreover, there was no difference between P and R group (p > 0.05). The geometric mean (GM) and 95% confidence interval (CI) of cadmium concentrations in W2 was 0.13 (0.01-1.72) microg l(-1) for U group, while for combined (P&R) non-urban group (NU) it was 0.31 (0.02-6.24) microg l(-1) (p < 0.001). Significant differences between cadmium in W1 and W2 samples were evident from the ratio of cadmium concentration in W1 to W2, i.e. R1,2; its difference (W1 - W2), i.e. D1,2; or relative difference 100 x (W1 - W2)/W1, i.e. D1,2%. For NU sites D1,2, D1,2% and R1,2 values (GM) were 0.25 microg l(-1), 34% and 2.19, respectively, and for U sites 0.19 microg l(-1), 50% and 3.49, respectively. Parameter D1,2 was not site dependent (p > 0.05) and exhibited a plateau at 0.44 microg l(-1). The WHO and Polish limit of cadmium in DW (3 microg l(-1)) was exceeded in W2 samples from 0.5, 5.8 and 10.0% of households from the U, P and R areas, respectively. The predicted cadmium intake with W2 by children was (GM) 0.68 and 2.06% of PTWI for U and NU sites, respectively. However, these contributions were higher for W1 samples by coefficient R1,2. For the cases exceeding the cadmium limit, the predicted cadmium intake with DW contributed to PTWI by 27.4-29.1%. The study proved that contamination of water with cadmium in plumbing is significant, independent of the site type and period. This contamination should be considered as a factor in public health protection programmes with special attention to children. An easy approach to limit the intake of cadmium from DW by about half would be to discard some of the first drawn portion of water from pipes after overnight or longer break of usage.

Anthropometry↗

A survey of aflatoxin levels in peanut meal imported into Poland for animal feedingstuffs.

Peanut meals (366 samples) imported into Poland from Brazil and India during 1984-86 for use in animal feedingstuffs were analysed for aflatoxins B1 and B2. Average levels of contamination were 71 micrograms per kg for aflatoxin B1 and 16 micrograms per kg for aflatoxin B2 with total concentrations ranging from 2 to 750 micrograms per kg.

Aflatoxin B1↗

Natural contamination of spring barley with group A trichothecene mycotoxins in south-eastern Poland.

Strains (10705) of microscopic fungi were isolated from spring barley heads in the region of Lublin (south-eastern Poland). Fusarium sporotrichioides Sherb was found in 418 (3.9%) of isolated strains. Group A trichothecene mycotoxins were detected in the collected barley kernels colonized by F. sporotrichioides, with Fusarium head blight symptoms. Among 24 samples analysed, 12 were T-2 toxin positive in a range of contamination from 0.02 to 2.40 micrograms/g (average 0.45), while in five samples HT-2 toxin ranged from 0.01 to 0.37 micrograms/g (average 0.23) and T-2 tetraol was detected in two samples in a range of 0.01-0.21 micrograms/g (average 0.11). Twelve samples were free of detectable amounts of the toxic metabolites analysed.

Chromatography, Gas↗

Poland's syndrome with rare deformities: reconstruction with latissimus dorsi muscle through a single short incision.

We describe a 22-year-old man with Poland's syndrome and two other rare deformities which, to our knowledge have not been reported previously. The first deformity was a fibrotic band between the nipple-areola complex and the medial epicondyle of the humerus, and the second was a fifth digit with two phalanges. A single midaxillary vertical incision was used to harvest and then transfer the latissimus muscle for soft tissue reconstruction of the chest wall deformity. Almost complete symmetry was achieved and the scar was well hidden by the arm. The aesthetic and functional results were satisfactory.

Adult↗

Variation in bone mineral density in adults in Poland: age and sex differences.

The decline of mineral bone density with age can lead in more extreme cases to osteopenia and osteoporosis, and is therefore one of the aspects of ageing with great medical and social significance. With this idea in mind a study of age changes in the trabecular and, separately, the cortical bone density of the radius was carried out in 1218 females and 405 males, aged 22 to 60 years, all occupationally active inhabitants of the city of Wroclaw, Poland. The technique used was the peripheral Quantitative Computed Tomography (pQCT). It was found that in females bone densities remain relatively stable throughout the period between 22 and 40 years. They then begin to decline slowly, with a rapid decline after the age of 55. A distinctly different pattern was found among males, with bone densities reaching peak values, markedly higher than those in females, in the third decade of life. After this age the bone density values begin to decline at a rapid rate, so that by the age of 60 years mean trabecular and cortical densities in males have decreased to levels almost equivalent to females of equal age. In view of the small size of the male samples, especially in the older age classes, the above results should be treated with caution and confirmed using larger samples.

Adult↗

An assessment of biological age of males in Poland.

BACKGROUND: Studies carried out in Poland have shown that some important indices of growth and maturation in children, of the biological well-being during adulthood and the rates of premature mortality depend strongly upon the individual's position on the social scale. AIM: The study considers whether adult males of higher educational status differ from their chronological age-matches of lower educational status in biological age. SUBJECTS AND METHODS: The data of 2800 occupationally active men, aged 25-65 from the 1994/1995 Polish Health Surveys were used. Twenty-two different measures were used. Biological age was assessed by the method of Borkan and Norris (Journal of Gerontology, 35, 177-184, 1980). RESULTS: A comparison of biological age profiles of two groups of males based on their educational status showed that in 13 of the 22 characteristics. better-educated men were biologically younger than their poorer educated peers (p < 0.05). CONCLUSION: Better education is associated with the slowing down of the process of ageing, probably because it produces a healthier life-style.

Adult↗

Menarcheal age and socio-economic factors in Poland.

BACKGROUND: Menarcheal age is the most important measure of sexual maturation in girls and a sensitive indicator of environmental conditions during childhood. OBJECTIVE: The study analysed the association between age at menarche and socio-economic characteristics (urbanization, population size, education of parents and number of children in the family). SUBJECTS AND METHODS: Questionnaire data were collected from 3271 female schoolchildren born between 1981 and 1984, living in three provinces of southern Poland. Menarcheal age was estimated by the recall method and based on the date of menarche given by the study participants. ANCOVA and multiple regression analyses were applied to test statistical significance of differences between groups. RESULTS: Girls from families with high socio-economic status experience menarche at an earlier age than girls from families with lower socio-economic status. However, depending on the geographical region and the population size, other factors influence menarcheal age. In the Krakow province, factors that significantly differentiate age at menarche are urbanization, father's education and number of children in the family; in the Opole province, these factors include urbanization and number of children in the family, while in the Nowy Sacz province, number of children in the family is significant. CONCLUSIONS: Socio-economic differences are greater in a large urban city (Krakow), and affect variation in age at menarche. However, within smaller populations (Opole, Nowy Sacz) living in towns and villages, the difference in age at menarche is less visible. In addition, variation between areas reveals a lower age at menarche in urban as compared with non-urban areas.

Adolescent↗

Lead and growth status of school children living in the copper basin of south-western Poland: differential effects on bone growth.

OBJECTIVE: The study analyzed the relationship between blood lead (BPb) levels and growth status of upper and lower body segments among schoolchildren living in the copper basin of south-western Poland. MATERIALS AND METHODS: Schoolchildren 7-15 years of age (463 males, 436 females) living in the vicinity of copper smelters and refineries were tested for blood lead. Weight, height, symphyseal height (leg, lower segment length), and arm length were measured. Trunk (upper segment including head and neck) length was estimated as height minus symphyseal height. The BMI was calculated using weight/height2 (kg m-2). RESULTS: Mean blood lead in the total sample was 7.7 +/- 3.5 micro g dL-1 (2.0-33.9 micro g dL-1). Blood lead level was significantly and linearly related to reduced weight, height, trunk, leg, and arm lengths. The estimated reduction in height was 5 mm per 1 micro g dL-1 increase in blood lead (5.0 cm per 10 micro g dL-1). The reduction occurred in leg length (males, 64%; females 72%) compared to trunk length (males, 36%; females, 28%). Estimated reductions in arm length were 2.0 cm (males) and 2.5 cm (females) per 10 micro g dL-1 increase in blood lead. Hence, limb (leg and arm) lengths were markedly reduced. CONCLUSIONS: Linear skeletal growth was reduced with increased blood lead, even at levels below that considered for action by US CDC standards. The reduction in height occurred primarily in leg length. Arm length was also reduced, suggesting limb lengths in general were affected by blood lead. Results are consistent with lead-associated interruption of (1) major epiphyseal growth plate chondrocyte hyperplasia, hypertrophy, and interrupted matrix calcification as a primary cause of stunted long bone growth, and reduced stature; and (2) attenuated osteoblast activity (proliferation and migration) as a secondary cause of stunted growth in the study population.

Adolescent↗

Social-class gradients in menarcheal age in Poland.

In a sample of approx. 19 000 Polish schoolgirls from the three largest cities of the Upper Silesia conurbation, menarcheal age was studied in relation to parental education (four levels) and father's occupation (12 groups). Menarcheal age tends to increase with decreasing parental education, although the gradient is not steep. When families below a certain level of economic standing are discarded from the best-educated and the least-well-educated groups, mean menarcheal age, surprisingly, decreases much more in the former than in the latter. Mean menarcheal ages for girls from different occupational groups range from 12.82 to 13.30 years and form the following sequence, in increasing order: managers--police--non-technical professionals--engineers, technicians and foremen--skilled industrial workers and small businessmen--unskilled workers--coal-miners. Mean menarcheal age for an occupational group is strongly dependent upon the group's socio-economic status, the latter being defined in terms of parental education, family income, family size, and dwelling conditions. However, daughters of men in the police force mature significantly earlier, and daughters of coal-miners significantly later, than would be expected from each group's rank in socio-economic status. The findings are compared with the results of other recent studies of social gradients in menarcheal age in Poland.

Adolescent↗

Deceleration of age at menarche in Poland.

The purpose of this paper is to describe secular growth changes in age at menarche of girls in Poland during the past 40 years. For this purpose data obtained from four consecutive surveys conducted in 1955, 1966, 1978 and 1988 were analysed. Each cohort was selected from settlements: big cities, small towns (approximately 10,000 inhabitants) and villages. Data regarding schoolgirls aged 7-18 years were collected by the status quo method. It was found that after the continuous trend towards an earlier maturation of Polish girls from 1955 to 1978 a substantial slowing down, and even a reverse trend, was observed. The deceleration of the age at menarche is most marked among girls from small towns. The results seem to have been caused by the retardation of menarcheal age in social groups which in the previous examinations were the earliest maturers.

Adolescent↗

Borrelia burgdorferi sensu lato infection in passerine birds from the Mazurian Lake region (Northeastern Poland).

The aim of the present study was to evaluate a potential role of different passerine birds species in Mazurian Lake region (northeast Poland) in the spread of Borrelia burgdorferi sensu lato, the spirochaete that causes Lyme disease. A total number of 1254 birds (representing 42 species) were captured during the 3-year study period. Blood samples were collected from birds and analyzed with a nested polymerase chain reaction technique in order to detect fragments of the pathogen DNA. Positive results were obtained in 4.2% of all blood samples. Specifically, B. burgdorferi s.l. were detected in tree pipit (Anthus Erivialis; 21.1% of 19 birds), dunnock (Prunella modularis; 15.8% of 19 birds), chaffinch (Fringilla coelebs; 12.7% of 166 birds), song thrush (Turdus philometos; 9.3% of 54 birds), nuthatch (Sitta euopea; 7.7% of 26 birds), hawfinch (Coccothraustes coccothroustes; 6.7% of 15 birds), robin (Erithacus rebecula; 5.1% of 256 birds), blackbird (Turdus merula; 4.2% of 71 birds) and wren (Troglodytes troglodytes; 3.7% of 27 birds). Additionally, the incidence of the infection was analyzed in relation to the habitat in which the birds resided (mixed coniferous forest or alder swamp forest), months of the study (from April to October), age and sex, but the differences were not statistically significant.

Age Factors↗

Environmental research on potential pesticides: indispensability and scope under conditions of Poland.

In consideration of the ubiquitous presence of pesticides in the environment, research should be done for each new compound aimed at evaluation of the hazard resulting from its application. Environmental investigations conducted in Poland encompass research on: (a) abiotic transformations (hydrolysis, photodegradation), (b) metabolism in plants and animals, as well as the fate in aquatic and soil environments; (c) influence on biota (including microflora, honey-bee and other beneficial arthropoda, aquatic organisms, birds); (d) side effects (bioaccumulation, resistance, subsequent action of sublethal amounts on populations); (e) mechanisms of action; (f) biological survey of sewage. The data obtained make possible an evaluation of risk connected with application of the investigated compound.

Animals↗