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At least 1,081 records · Page 60Linked to original sources
PubMed1980

Experimental lead paint poisoning in nonhuman primates. II. Clinical pathologic findings and behavioral effects.

Oral administration of lead-containing paint to rhesus monkeys induced anemia, more profound in older primates. Erythrocytes were microcytic and hypochromic, but tended to become macrocytic terminally. Stippled erythrocytes were increased in all poisoned monkeys, especially in those with high blood lead levels and anemia. Proteinuria, glycosuria, casts and sloughed tubular cells containing acid-fast inclusion bodies were found on urinalysis. Terminal elevations of blood urea nitrogen were associated with profound anemia and renal tubular damage. Repeated blood lead values over 200 microgram/dl were associated with a moribund termination while monkeys which had levels under 100 microgram/dl remained apparently healthy. Behavioral studies in a small number of subclinically poisoned juveniles and neonates failed to reveal deficiencies of visual acuity or cognitive ability, nor was there evidence of alterations in levels of activity.

Anemia↗
PubMed1980

Finger painting: a projective technique.

Finger painting was used in a diversified program with two different groups of patients, primarily as a medium for expression of feeling that facilitated the collection of diagnostic data and the detection of change in mental status. It was found to be an economical method of eliciting data and monitoring change during therapy. In this technique the patient produces his own material and verbally associates to it thus giving a more objective profile of personality and psychopathology. Perusal of the literature revealed no recent studies of the technique and prompted a documentation of this project at the Reddy Memorial Hospital. The technique proved itself a worthwhile method of eliciting data but needs to be scientifically evaluated. Attempts should also be made to assess its ability to discriminate between different diagnostic categories in psychiatry.

Art Therapy↗
PubMed1977

The use of betadine antiseptic paint in the treatment of Herpes simplex and Herpes Zoster.

Twenty-two patients with Herpes Simplex were treated with Betadine (povidone iodine) Antiseptic paint and seven with 5% idoxuridine in dimethyl sulphoxide, also one patient with bilateral lesions was treated with the two solutions, one on each side. The povidone iodine alcohol solution appeared to be at least as effective as the idoxuridine with minimal side-effects (some stinging in two patients). Good results were also obtained with this solution in twelve patients with Herpes Zoster.

Adolescent↗
PubMed1984

The effect of povidone-iodine paint on fungal infection.

Povidone-Iodine (Betadine) Paint was used in thirteen patients with a proven fungal infection. Ten patients had pityriasis versicolor, two had trichophyton rubrum and one M. canis. In the pityriasis versicolor group, seven out of the ten patients were either improved or cleared up completely within 7 days. There were no adverse reactions in this group of patients and the material proved quite acceptable to them. Scanning electron micrographs were carried out on three of the patients and demonstrated the decrease in fungal elements after treatment and clinical improvement.

Humans↗
PubMed2003

Endogenous and exogenous ice-nucleating agents constrain supercooling in the hatchling painted turtle.

Hatchlings of the painted turtle (Chrysemys picta) commonly hibernate in their shallow, natal nests. Survival at temperatures below the limit of freeze tolerance (approximately -4 degrees C) apparently depends on their ability to remain supercooled, and, whereas previous studies have reported that supercooling capacity improves markedly with cold acclimation, the mechanistic basis for this change is incompletely understood. We report that the crystallization temperature (T(c)) of recently hatched (summer) turtles acclimated to 22 degrees C and reared on a substratum of vermiculite or nesting soil was approximately 5 degrees C higher than the T(c) determined for turtles acclimated to 4 degrees C and tested in winter. This increase in supercooling capacity coincided with elimination of substratum (and, in fewer cases, eggshell) that the hatchlings had ingested; however, this association was not necessarily causal because turtles reared on a paper-covered substratum did not ingest exogenous matter but nevertheless showed a similar increase in supercooling capacity. Our results for turtles reared on paper revealed that seasonal development of supercooling capacity fundamentally requires elimination of ice-nucleating agents (INA) of endogenous origin: summer turtles, but not winter turtles, produced feces (perhaps derived from residual yolk) that expressed ice-nucleating activity. Ingestion of vermiculite or eggshell, which had modest ice-nucleating activity, had no effect on the T(c), whereas ingestion of nesting soil, which contained two classes of potent INA, markedly reduced the supercooling capacity of summer turtles. This effect persisted long after the turtles had purged their guts of soil particles, because the T(c) of winter turtles reared on nesting soil (mean +/- S.E.M.=-11.6+/-1.4 degrees C) was approximately 6 degrees C higher than the T(c) of winter turtles reared on vermiculite or paper. Experiments in which winter turtles were fed INA commonly found in nesting soil showed that water-soluble, organic agents can remain fully active for at least one month. Such INA may account for the limited supercooling capacity (T(c) approximately -7.5 degrees C) we found in turtles overwintering in natural nests and may therefore pose a formidable challenge to the winter survival of hatchling C. picta.

Acclimatization↗
PubMed2004

Time-dependent expression of heat shock proteins 70 and 90 in tissues of the anoxic western painted turtle.

Expression of the constitutive Hsp73, inducible Hsp72 and Hsp90 was investigated in brain, heart, liver and skeletal muscle of the anoxia-tolerant western painted turtle Chrysemys picta bellii in response to 2, 6, 12, 18, 24 and 30 h forced dives and following 1 h recovery from 12, 24 and 30 h forced dives at 17 degrees C. During a dive, expression of all three Hsps examined remained at control levels for at least 12 h in all tissues examined except the liver, where Hsp72 showed a decrease at 12 h, reaching a significant threefold decrease by 24 h. Brain and liver Hsp73, 72 and 90 expression increased two- to threefold at 18, 24 and 30 h. Heart and muscle Hsp73 and heart Hsp90 expression remained at normoxic levels throughout the entire dive, while heart and muscle Hsp72 and muscle Hsp90 increased two- to fourfold at 24 and 30 h. Following reoxygenation, Hsp expression increased in all tissues examined. These data indicate that increased Hsp expression is not critical in the early adaptation to anoxic survival and that short-term anoxia is probably not a stress for species adapted to survive long periods without oxygen. However, the late upregulation of heat shock proteins during anoxia suggests that stress proteins play a role in promoting long-term anoxia tolerance.

Analysis of Variance↗
PubMed2005

Patterns of variation in glycogen, free glucose and lactate in organs of supercooled hatchling painted turtles (Chrysemys picta).

Hatchling painted turtles (Chrysemys picta) typically spend their first winter of life in a shallow, subterranean hibernaculum (the natal nest), where they may be exposed for extended periods to ice and cold. The key to their survival seems to be to avoid freezing and to sustain a state of supercooling. As temperature declines below 0 degrees C, however, the heart of an unfrozen turtle beats progressively slower, the diminished perfusion of peripheral tissues with blood induces a functional hypoxia, and anaerobic glycolysis assumes ever greater importance as a source of ATP. We hypothesized that diminished circulatory function in supercooled turtles also reduces the delivery of metabolic substrates to peripheral tissues from central stores in the liver, so that the tissues depend increasingly on endogenous stores to fuel their metabolism. We discovered in the current investigation that part of the glycogen reserve in hearts and brains of hatchlings is mobilized during the first 10 days of exposure to -6 degrees C but that glucose from hepatic glycogen supports metabolism of the organs thereafter. Hatchlings that were held at -6 degrees C for 10 days and then at +3 degrees C for another 10 days were able to reconstitute some of the reserve of glycogen in heart and liver but not the glycogen reserve in brain. Patterns of accumulation of lactate in individual organs were very similar to those reported for whole animals in a companion study, and point to a high degree of reliance on anaerobic metabolism at -6 degrees C and to a lesser degree of reliance on anaerobiosis at higher subzero temperatures. Lactate had returned to baseline levels in organs of animals that were held for 10 days at -6 degrees C and for another 10 days at +3 degrees C, but free glucose remained elevated. Indeed, carbohydrate metabolism probably does not return to the pre-exposure state in any of the major organs until well after the exposure to subzero temperatures has ended, circulatory sufficiency has been restored, and aerobic respiration has fully supplanted anaerobic respiration as a source of ATP.

Analysis of Variance↗
PubMed2006

Aquatic turning performance of painted turtles (Chrysemys picta) and functional consequences of a rigid body design.

The ability to capture prey and avoid predation in aquatic habitats depends strongly on the ability to perform unsteady maneuvers (e.g. turns), which itself depends strongly on body flexibility. Two previous studies of turning performance in rigid-bodied taxa have found either high maneuverability or high agility, but not both. However, examinations of aquatic turning performance in rigid-bodied animals have had limited taxonomic scope and, as such, the effects of many body shapes and designs on aquatic maneuverability and agility have yet to be examined. Turtles represent the oldest extant lineage of rigid-bodied vertebrates and the only aquatic rigid-bodied tetrapods. We evaluated the aquatic turning performance of painted turtles, Chrysemys picta (Schneider, 1783) using the minimum length-specific radius of the turning path (R/L) and the average turning rate (omega(avg)) as measures of maneuverability and agility, respectively. We filmed turtles conducting forward and backward turns in an aquatic arena. Each type of turn was executed using a different pattern of limb movements. During forward turns, turtles consistently protracted the inboard forelimb and held it stationary into the flow, while continuing to move the outboard forelimb and both hindlimbs as in rectilinear swimming. The limb movements of backward turns were more complex than those of forward turns, but involved near simultaneous retraction and protraction of contralateral fore- and hindlimbs, respectively. Forward turns had a minimum R/L of 0.0018 (the second single lowest value reported from any animal) and a maximum omega(avg) of 247.1 degrees. Values of R/L for backward turns (0.0091-0.0950 L) were much less variable than that of forward turns (0.0018-1.0442 L). The maneuverability of turtles is similar to that recorded previously for rigid-bodied boxfish. However, several morphological features of turtles (e.g. shell morphology and limb position) appear to increase agility relative to the body design of boxfish.

Animals↗
PubMed1998

Soil hydric characteristics and environmental ice nuclei influence supercooling capacity of hatchling painted turtles Chrysemys picta.

Hatchling painted turtles (Chrysemys picta) hibernate in their shallow natal nests where temperatures occasionally fall below -10 C during cold winters. Because the thermal limit of freeze tolerance in this species is approximately -4 C, hatchlings rely on supercooling to survive exposure to extreme cold. We investigated the influence of environmental ice nuclei on susceptibility to inoculative freezing in hatchling C. picta indigenous to the Sandhills of west-central Nebraska. In the absence of external ice nuclei, hatchlings cooled to -14.6 1.9 C (mean s.e.m.; N=5) before spontaneously freezing. Supercooling capacity varied markedly among turtles cooled in physical contact with sandy soil collected from nesting locales or samples of the native soil to which water-binding agents (clay or peat) had been added, despite the fact that all substrata contained the same amount of moisture (7.5 % moisture, w/w). The temperature of crystallization (Tc) of turtles exposed to frozen native soil was -1.6 0.4 C (N=5), whereas turtles exposed to frozen soil/clay and soil/peat mixtures supercooled extensively (mean Tc values approximately -13 C). Hatchlings cooled in contact with drier (less than or equal to 4 % moisture) native soil also supercooled extensively. Thus, inoculative freezing is promoted by exposure to sandy soils containing abundant moisture and little clay or organic matter. Soil collected at turtle nesting locales in mid and late winter contained variable amounts of moisture (4-15 % w/w) and organic matter (1-3 % w/w). In addition to ice, the soil at turtle nesting locales may harbor inorganic and organic ice nuclei that may also seed the freezing of hatchlings. Bulk samples of native soil, which were autoclaved to destroy any organic nuclei, nucleated aqueous solutions at approximately -7 C (Tc range -6.1 to -8.2 C). Non-autoclaved samples contained water-extractable, presumably organic, ice nuclei (Tc range -4.4 to -5.3 C). Ice nuclei of both classes varied in potency among turtle nesting locales. Interaction with ice nuclei in the winter microenvironment determines whether hatchling C. picta remain supercooled or freeze and may ultimately account for differential mortality in nests at a given locale and for variation in winter survival rates among populations.

Animals↗
PubMed2000

Seasonal changes in physiology and development of cold hardiness in the hatchling painted turtle Chrysemys picta.

Hatchling painted turtles (Chrysemys picta) commonly hibernate in shallow, natal nests where winter temperatures may fall below -10 degrees C. Although hatchlings are moderately freeze-tolerant, they apparently rely on supercooling to survive exposure to severe cold. We investigated seasonal changes in physiology and in the development of supercooling capacity and resistance to inoculative freezing in hatchling Chrysemys picta exposed in the laboratory to temperatures that decreased from 22 to 4 degrees C over a 5.5 month period. For comparison, we also studied hatchling snapping turtles (Chelydra serpentina), a less cold-hardy species that usually overwinters under water. Although Chrysemys picta and Chelydra serpentina differed in some physiological responses, both species lost dry mass, catabolized lipid and tended to gain body water during the acclimation regimen. Recently hatched, 22 degrees C-acclimated Chrysemys picta supercooled only modestly (mean temperature of crystallization -6.3+/-0.2 degrees C; N=6) and were susceptible to inoculation by ice nuclei in a frozen substratum (mean temperature of crystallization -1.1+/-0.1 degrees C; N=6) (means +/- s.e.m.). In contrast, cold-acclimated turtles exhibited pronounced capacities for supercooling and resistance to inoculative freezing. The development of cold hardiness reflected the elimination or deactivation of potent endogenous ice nuclei and an elevation of blood osmolality that was due primarily to the retention of urea, but was not associated with accumulation of the polyols, sugars or amino acids commonly found in the cryoprotection systems of other animals. Also, Chrysemys picta (and Chelydra serpentina) lacked both antifreeze proteins and ice-nucleating proteins, which are used by some animals to promote supercooling and to initiate freezing at the high temperatures conducive to freezing survival, respectively.

Acclimatization↗
PubMed2003

Synthesis of the four components of the female sex pheromone of the painted apple moth, Teia anartoides.

Four pheromone components of the female painted apple moth (Teia anartoides), an Australian insect pest, were synthesized. These were (Z)-6-henicosen-11-one (1), (6Z, 8E)-6,8-henicosadien-11-one (2), (Z)-cis-9,10-epoxy-6-henicosene (3), and (Z)-cis-9,10-epoxy-6-icosene (4). 2-Dodecanone was converted to 1 and 2, and both the enantiomers of 3 and 4 were synthesized from the enantiomers of 4-tert-butyldimethylsilyloxy-cis-2,3-epoxy-1-butanol.

Animals↗
PubMed1999

Pathogenicity of Mycobacterium avium complex serovar 9 isolated from painted quail (Excalfactoria chinensis).

Avian tuberculosis accompanied with many tubercular lesions in the liver and spleen was found in a painted quail at a zoological garden in Japan. Mycobacterium avium complex (MAC) serovar 9 without insertion sequence of IS901 was isolated from the liver (1.3 x 10(8) CFU/g), oviduct (9.4 x 10(7) CFU/g), and intestine (1.5 x 10(5) CFU/g). The isolates were inoculated intravenously to chickens. The inoculated chickens showed clinical symptoms of avian tuberculosis. Birds are susceptible to MAC serovar 9 without IS901.

Agglutination Tests↗
PubMed2002

Painting, poetry and optics: Johannes Vermeer.

The painter Johannes Vermeer (1632-75) worked in the town of Delft in the Dutch Republic. This article focuses on associations between his painting method and the expertise in optics available in Delft at the time. His art represents an extraordinary combination of technical prowess and poetic expression.

Art↗
PubMed2000

Helminth parasites of the western painted turtle, Chrysemys picta belli (Gray), including Neopolystoma elizabethae n. sp. (Monogenea: Polystomatidae), a parasite of the conjunctival sac.

Neopolystoma elizabethae n. sp. is described from the conjunctival sac of the western painted turtle Chrysemys picta belli (Gray), from the Upper Peninsula of Michigan. This is the first species found in this location from chelonians in North America. The new species differs from all other species of Neopolystoma in possessing a circle of 8 genital spines that are recurved and possess a crescent-shaped base. Eight additional species of helminths were found in the 5 turtles examined in this study. All are common parasites of North American freshwater turtles. An additional species of Monogenea (Polystomoidespauli) was found in the oral cavity. Four species of Digenea (Eustomos chelydrae, Allassostomoides chelydrae, Spirorchis kirki, and Spirorchis parvus) and 3 species of Nematoda (Spiroxys contorta, Serpinema trispinosus, and Amphibiocapillaria serpentina) were also found. The following are reported from Michigan for the first time: P. pauli, S. kirki, and A. serpentina.

Animals↗
PubMed2001

Transmission of Haemogregarina balli from painted turtles to snapping turtles through the leech Placobdella ornata.

Six leeches (Placobdella ornata) were allowed to feed on a painted turtle (Chrysemys picta marginata) infected with Haemogregarina balli and subjected to a period of diapause before being allowed to feed on 2 laboratory-reared snapping turtles (Chelydra serpentina). Weekly examination of thin blood films revealed infections of the turtles at 130 days postfeeding. These observations provide support for broad host specificity of hemogregarine parasites of chelonians.

Animals↗
PubMed2005

In situ hybridization by scanning electron microscopy for painting, centromeric, and YAC localization.

The hybridization site of a DNA probe was detected using a scanning electron microscope (SEM), modifying the standard in situ hybridization (ISH) method. The experiments were performed on human metaphases obtained from lymphocyte cultures of human peripheral blood. The libraries and probes used were: 1-chromosome library for the painting of chromosome 1 (wcp 1), an alphoid centromere-specific probe of chromosome 8 (pZ8.4), and the yeast artificial chromosome (YAC) 964-C10 mapped at band p13 on chromosome 12. These probes were labeled by nick translation with biotin and displayed with a gold-conjugated anti biotin goat antibody. The gold signal was amplified by silver enhancement. The chromatides appeared as packages of thin filaments 120 nm high; some of them collapsed, probably due to ISH procedures. All the probes were clearly detected as small gold particles grouped on the surface of the target chromosomes and chromosome sites. Thus, this procedure is useful to clarify the positional relationship between the chromatin filaments and the probe.

Centromere↗
PubMed1981

Demonstration of dnp groups on the draining lymph node cells of guinea pigs following skin painting with DNCB by peroxidase labelled antibody method.

The distribution of 2,4-dinitrophenyl (DNP) groups in the draining lymph nodes of guinea pigs 12 h after painting the skin with 2,4-dinitrochlorobenzene (DNCB) was examined by a peroxidase labelled antibody method using antibody against DNP groups. DNP groups were detected on cells that were found mainly in the subcapsular sinus of the lymph nodes. Electron microscopic examination showed DNP groups distributed on the surface of lymphocytes. The significance of these findings is discussed.

Animals↗
PubMed1990

A case report of lead paint poisoning during renovation of a Victorian farmhouse.

We describe a series of four cases of childhood lead poisoning and two cases of adult lead toxicity in a professional family exposed to lead dust and fume during renovation of a rural farmhouse. Initial blood lead levels in the children ranged from 2.70 to 4.20 microM/L (56 to 87 microns/dl) and all four required chelation therapy. Lead-based paint poisoning, a well recognized entity among young children in poor, urban neighborhoods, is not confined exclusively to such areas.

Adult↗