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PubMed2004

Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces.

Aeromonas spp. (gram-negative, aquatic bacteria which include enteropathogenic strains) have two distinct flagellar systems, namely a polar flagellum for swimming in liquid and multiple lateral flagella for swarming over surfaces. Only approximately 60% of mesophilic strains can produce lateral flagella. To evaluate flagellar contributions to Aeromonas intestinal colonization, we compared polar and lateral flagellar mutant strains of a diarrheal isolate of Aeromonas caviae for the ability to adhere to the intestinal cell lines Henle 407 and Caco-2, which have the characteristic features of human intestinal enterocytes. Strains lacking polar flagella were virtually nonadherent to these cell lines, while loss of the lateral flagellum decreased adherence by approximately 60% in comparison to the wild-type level. Motility mutants (unable to swim or swarm in agar assays) had adhesion levels of approximately 50% of wild-type values, irrespective of their flagellar expression. Flagellar mutant strains were also evaluated for the ability to form biofilms in a borosilicate glass tube model which was optimized for Aeromonas spp. (broth inoculum, with a 16- to 20-h incubation at 37 degrees C). All flagellar mutants showed a decreased ability to form biofilms (at least 30% lower than the wild type). For the chemotactic motility mutant cheA, biofilm formation decreased >80% from the wild-type level. The complementation of flagellar phenotypes (polar flagellar mutants) restored biofilms to wild-type levels. We concluded that both flagellar types are enterocyte adhesins and need to be fully functional for optimal biofilm formation.

Adhesins, Bacterial↗
PubMedDate not supplied

Therapy for thrombotic occlusion of long-term intravenous alimentation catheters.

Thrombosis and infection are closely related complications of implanted silastic catheters. A procedure, by which the thrombi are dispersed with urokinase while the catheter remains in situ, was uniformly successful in clearing the line in 20 patients, although in 5 the infusion had to be repeated 24 hr later. Catheter-associated infections are rendered susceptible to antibiotics as the infected thrombotic nidus is lysed and the organisms exposed. In 7 patients, 4 of whom were taking antibiotics, fever and blood cultures became negative. The procedure results in no detectable systemic fibrinolysis or other complications.

Catheters, Indwelling↗
PubMed1976

[Apex carotis diagram and benign systolic murmurs].

The apex-carotis diagram is a new mechanocardiographic, non invasive method consisting of integration of the carotidogram and apexcardiogram--synchronously recorded. The value of the amplitude height of these curves at every 0.025 sec are transferred to an orthogonal coordinate system and later the points connected in a chronological order thus obtaining the apex-carotidogram (ACD). ACD have been constructed in 62 healthy subjects and in 26 people with innocent systolic murmur, aged between 14--65 years. Our investigation showed no difference between the control group and the people with innocent murmur. The points of connection, lines, segments, fields and axis of ACD were the same in both groups. The angles alpha, beta, gamma, delta, epsilon measured quantity and calculated showed no statistical difference (p greater than 0.05).

Adolescent↗
PubMed2000

[Epistaxis: emergency treatment approach].

After a brief summary of the causes of severe epistaxis and the surgical choices available for treatment, the authors present their own cases study. Starting from a brief description of the anatomy involved in the system of arterial irrigation of the nasal cavities, they focus on two fundamental concepts: the basis for a proper topographic picture of the site of bleeding and the success of surgery. First and foremost is the emergence of two arterial branches from the sphenopalatine formen; this differs from what is asserted in the classical treatise on anatomy and in line with what has been stated in the Nomina Anatomica, approved during the X World Congress on Anatomy (Tokyo 1975). Secondly, focus is placed on the existence of two important anastomotic systems in the arterial circulation of the nasal cavities: the one between nasal septum and anterior-posterior ethmoid arteries and the one between the lateral-posterior nasal and nasoseptal arteries. In the literature the failure of surgery in treating severe epistaxis is explained by the onset of supplementary anastomotic circulation. The indications for surgical treatment of epixstaxes are: copious bleeding in hospital or after the packing has been removed; underlying pathology or general status which has been aggravated by the packing; patient which has already been staunched several times. The results of 31 epistaxis procedures are presented: 8 (25.81%) upper and 23 (74.19%) posterior. The upper epistaxes were treated as follows: 3 cases with anterior ethmoid ligature, two of which failed; 2 cases with combined ligature of the anterior and posterior ethmoid; 3 cases of successful septoplasty. The posterior epistaxes were treated as follows: 11 cases of ligature of the lateral-posterior nasal artery, 2 of which failed; 12 cases of successful combined ligature of lateral-posterior nasal and nasoseptal arteries. These results confirm that it is possible to effectively resolve surgically severe epistaxis using microsurgery or transnasal endoscopy based on in depth knowledge of the complex architecture of the arterial circulation within the district of the paranasal sinuses, possible anatomic variations and collateral circulation.

Adult↗
PubMed1993

[High dose chemoradiotherapy and autologous bone marrow transplantation (ABMT) as a first line therapy to treat advanced adult high-grade malignant T-cell non-Hodgkin's lymphoma--a pilot clinical study].

Four patients with advanced adult high-grade malignant T-cell non-Hodgkin's lymphoma (NHL) were treated with high dose chemoradiotherapy and ABMT as a first line therapy. Case 1 was of stage IIIb; case 2, 3 and 4 were of stage IV. Bone marrow was involved (19% blast cells) in case 2, and nervous system was involved in case 4. All of them were given at first an inductive chemotherapy with 3-4 courses of BACOP regimen before ABMT. 40-50 days later, Vp-16, CTX and TBI were given as a conditioning regimen (case 1: TLI). Case 1 and 2 only reached partial remission after inductive treatment (case 2: relapse at primary sites one month after inductive therapy, but complete remission (CR) of bone marrow). All of the four patients reached CR after ABMT. Case 2 had systemic relapse (bone marrow blast cells: 8%) at 110 days after CR and died from relapse 210 days after ABMT. So far, case 1, 3 and 4 have survived free of disease for 24, 19 and 9 months, respectively. There was no fatal marrow transplant regimen-related toxicity during inductive and ABMT treatment. The results of this pilot study suggest that treatment of high-grade malignant NHL may be improved by combined use of high dose chemoradiotherapy and ABMT, and the toxicities can be tolerated.

Adolescent↗
PubMed1998

The early expression of Rev-erbbeta occurs in the developing nervous system of mouse embryo.

The orphan ligand nuclear receptor Rev-erbbeta acts in vitro as a negative regulator of transcription. However, its precise physiological role is still unknown. As a first attempt to better understand its biological function, we have studied the distribution and the localization of the Rev-erbbeta mRNA transcripts in different mouse embryonal carcinoma cell lines, in mouse embryos and adult tissues. Our results indicated that Rev-erbbeta transcripts are present in both the non-differentiated and differentiated F9 cells into either parietal or visceral endoderm. At 12.5 days of gestation (E12.5) of mouse embryos, Rev-erbbeta transcripts were localized only in the developing nervous system. In contrast, at later stages of development as well as in the adult, its messengers were widely distributed. These results suggest that Rev-erbbeta may have different roles at the different stages of mouse development, with a more specific role in the nervous system at earlier stages.

Animals↗
PubMed2000

Oncogenic Raf-1 disrupts epithelial tight junctions via downregulation of occludin.

Occludin is an integral membrane protein of the epithelial cell tight junction (TJ). Its potential role in coordinating structural and functional events of TJ formation has been suggested recently. Using a rat salivary gland epithelial cell line (Pa-4) as a model system, we have demonstrated that occludin not only is a critical component of functional TJs but also controls the phenotypic changes associated with epithelium oncogenesis. Transfection of an oncogenic Raf-1 into Pa-4 cells resulted in a complete loss of TJ function and the acquisition of a stratified phenotype that lacked cell-cell contact growth control. The expression of occludin and claudin-1 was downregulated, and the distribution patterns of ZO-1 and E-cadherin were altered. Introduction of the human occludin gene into Raf-1-activated Pa-4 cells resulted in reacquisition of a monolayer phenotype and the formation of functionally intact TJs. In addition, the presence of exogenous occludin protein led to a recovery in claudin-1 protein level, relocation of the zonula occludens 1 protein (ZO-1) to the TJ, and redistribution of E-cadherin to the lateral membrane. Furthermore, the expression of occludin inhibited anchorage-independent growth of Raf-1-activated Pa-4 cells in soft agarose. Thus, occludin may act as a pivotal signaling molecule in oncogenic Raf- 1-induced disruption of TJs, and regulates phenotypic changes associated with epithelial cell transformation.

Animals↗
PubMed2000

The validity of the ECLAM index for the retrospective evaluation of disease activity in systemic lupus erythematosus.

AIM: To determine whether the European Consensus Lupus Activity Measurement Index (ECLAM) can be used to evaluate disease activity in patients retrospectively from the data provided in their clinical charts. METHODS: The ECLAM score was calculated twice in a series of 64 consecutive SLE patients: first for each patient during the course of a standard clinical evaluation (direct-ECLAM), and then one to two weeks later solely on the basis of the data provided in the patient's clinical chart (chartECLAM). The scorings for each patient were performed by two different assessors. RESULTS: The direct-ECLAM and chart-ECLAM scores were highly correlated (Spearman's rank correlation coefficient = 0.86). The regression line was not significantly different from the identity line (t-test). The Pearson's coefficient was 0.88. The interobserver variability of the chart-ECLAM showed a low inter-rater variability. CONCLUSION: ECLAM could represent a valid and reliable instrument for the retrospective analysis of disease activity in SLE patients.

Disease Progression↗
PubMed1997

Glial cell line-derived neurotrophic factor: distribution and pharmacology in the rat following a bolus intraventricular injection.

Glial cell line-derived neurotrophic factor (GDNF) has been shown to increase dopaminergic parameters in vitro and in vivo and can reduce parkinsonian behaviors in animal models of the disease. This study determined the potential of the lateral ventricle as an administration route for GDNF by examining the distribution and neurochemical consequences of a single intraventricular injection. Autoradiographic analysis showed that intraventricularly administered [125I]GDNF was distributed throughout the ventricular system at 1 and 24 h following injection. The cerebral cortex, septum, diagonal band, fimbria, striatum, hippocampus, hypothalamus, substantia nigra/ventral tegmental area, and cerebellum were also labeled. At 7 days, there was still labeling throughout the ventricular system, hypothalamus, substantia nigra, and cerebellum. Twenty-four hours following an intrastriatal injection of [125I]GDNF, label was observed in the substantia nigra/ventral tegmental area, demonstrating retrograde transport. The neurochemical effects of intraventricularly administered GDNF (0.1-100 micrograms) at 7 days post injection were also examined. GDNF significantly increased striatal (approximately 28%) and nigral (up to 40%) dopamine, as well as regulated the dopamine metabolites homovanillic acid and dihydroxyphenylacetic acid. Dopamine levels were unchanged in the frontal cortex. Dopamine content was significantly increased in the hypothalamus (up to 35%), an increase which may contribute to the inhibition of weight gain seen after administration of GDNF. Additionally, dopamine turnover was decreased or unchanged across the brain regions analyzed, which may indicate that in unlesioned rats, intraventricularly administered GDNF stimulates the synthesis and storage of dopamine. This study shows that intraventricularly injected GDNF can access basal ganglia structures, most notably the midbrain dopamine cell body region, and remains present in this area for at least 7 days following a single administration. GDNF differentially increases dopaminergic tone within a variety of brain structures, including the nigrostriatal pathway. These data support the potential effectiveness of intraventricular administered GDNF as a treatment for Parkinson's disease.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1985

B lymphocyte regulation of the immune system. I. In vivo biologic activity of the novel lymphokine, B cell-derived enhancing factor (BEF).

B cell-derived enhancing factor (BEF) is a lymphokine of B cell origin which was originally identified and characterized by its ability to enhance in vitro antibody responses, an effect shown to be due to the ability of BEF to reduce the activation of suppressor T cells. The present study was undertaken to determine whether BEF could also be active in modulating antibody responses in vivo. The data presented here demonstrate that BEF is biologically active in vivo, as manifested by significantly enhanced primary IgM and IgG antibody responses in mice that were either injected with BEF prepared exogenously or implanted with growing BEF-secreting cells of a B cell line. Moreover, BEF was shown to enhance subsequent development of immunologic memory in mice pretreated with BEF at the time of primary immunization; these mice then displayed enhanced secondary responses when challenged with the same antigen some weeks later. The mechanism by which BEF exerts biologic activities to positively modulate in vivo antibody responses and immunologic memory reflects the ability of BEF to modulate one or more T cell functions, as evidenced by the following findings. 1) Transient in vitro exposure to BEF of T cells, but not of B cells, endowed such cells with the capacity to adoptively transfer enhanced primary antibody responses to irradiated recipients. 2) Utilizing adoptive in vivo antibody responses, in which fractionated B cell or T cell populations were obtained from BEF-pretreated mice, revealed that one effect of BEF which results in enhanced immunologic memory is related to its activity on T cells during the priming phase of the immune response. Finally, the existence of this B cell-derived lymphokine and the demonstration of its in vivo regulatory effects on the immune system provide yet another example of the emerging biologic importance of B lymphocytes in the overall regulation of the immune system.

Animals↗
PubMed2004

In vivo 3D MRI staining of the mouse hippocampal system using intracerebral injection of MnCl2.

The morphology and function of the hippocampal system of C57BL/6J mice (n = 8) was studied in vivo using T1-weighted 3D magnetic resonance imaging (MRI) (117 microm isotropic resolution) after bilateral injection of MnCl(2) (0.25 microl, 5 or 200 mM) into the posterior hippocampal formation. The neuronal uptake of the T1-shortening Mn(2+) ions resulted in a pronounced MRI signal enhancement within the CA3 subfield and dentate gyrus with milder increases in CA1 and subiculum. This finding is in line with differences in the excitability of hippocampal neurons previously reported using electrophysiologic recordings. The subsequent axonal transport of Mn(2+) highlighted the principal extrinsic projections from the posterior hippocampal formation via the fimbria and the precommissural fornix to the dorsal part of the lateral septal nucleus. A strong MRI signal enhancement was also observed in the ventral hippocampal commissure. A time-course analysis revealed unsaturated conditions of Mn(2+) accumulation at about 2 h after injection and optimal contrast-to-noise ratios at about 6 h after injection. The present results using Mn(2+)-enhanced 3D MRI open new ways for studying the role of the hippocampal system in specific aspects of learning and memory in normal and mutant mice.

Animals↗
PubMed1987

Quantitative microscopy: I. A computer-assisted approach to the study of polymorphonuclear leukocyte (PMN) chemotaxis.

A computer-assisted approach has been designed to analyze and quantitate polymorphonuclear leukocyte (PMN) chemotaxis. This approach involves a rapid, objective, and semiautomated (user-directed) image-analysis system that is video- and microscope-based. The entire system consists of a microvideo set-up that is put on line with a Digital DEC-LSI-11/73 microcomputer, interfaced with a Datacube analog-digital/digital-analog converter. Video signals of PMN movement are digitized by the system at a resolution of 240 pixels vertically by 320 pixels horizontally (at 256 gray levels) and stored in a 76,800-byte frame buffer. The digitized data are stored for later use or utilized immediately for image segmentation, image display, movement, and morphometric computations for each PMN in a maximum phase field (at 645 X high dry) of 50 PMNs at 10-second intervals. The digitized data are used for computation of cell perimeter, surface area, optical density, contour-ratio, position, speed, and direction of locomotion with the utilization of micro-image-analysis programs written in FORTRAN and MACRO assembly language, with the computer operating under RT-11/TSX+. The reliability, objectivity, and reproducibility of measurements made with this quantitative approach have been tested by comparing with manual-tracing measurements of PMN movement. A correlation factor of 0.99 has been obtained. However, the quantitative-microscopic approach is much faster, more objective, less tedious, and much easier to operate than the conventional manual-tracing method.

Chemotaxis, Leukocyte↗
PubMed1978

Representation of complex visual stimuli in the brain.

A method was developed to investigate transfer properties of neurons in the visual system using pictures of complex visual stimuli. The picture is moved over the receptive field of a neuron so that it can scan it along programmed lines. The activity of the neuron during the scanning procedure is presented in a two-dimensional dot display on scale with the original picture. By superposition of the stimulus and the transfer pattern, one can find out to which detail of a stimulus the neuron responds. Neurons in the first intracerebral relay of the visual system, the lateral geniculate body, reduce a complex stimulus, such as a photograph of a natural environment, to its contours. Cortical cells only respond to contours either of a limited or of a wider range of orientations (simple and complex cells, respectively). But the course of contours is only described by a continuous representation of these contours in the cortical map of the visual field. This is done by the simple cells, which have small receptive fields and thus a higher resolving power, whereas complex cells with their large receptive fields monitor the approximate location of a moving stimulus. The function of these two classes of neurons is discussed in terms of visual behavior, i.e., for fixation, hold, and binocular vergence movements (simple cells), and for detection of moving objects and motor command signals towards these objects (complex cells). These functions are an important condition for foveal vision which is the basis of perception in primates. An important function of orientation sensitivity of simple cells may be the binocular alignment of contours in binocular fusion and stereoscopic vision.

Animals↗
PubMed1975

A computerized neurosurgical intensive care system.

A specialized neurosurgical data acquisition and processing system was developed and applied in practice to monitor some 60 patients after head injury or neurosurgical operations. At first the system allowed off-line operation, and the experiences thus gained made it possible to implement the system in its present form for real-time on-line patient monitoring. The algorithms prepared in our laboratory allow rapid and concise presentation of results and have proved their usefulness in clinical practice. The main parameters of interest in neurosurgery are intracranial pressure (ICP) and the volume of cerebrospinal fluid (CSF) drained from one of the lateral ventricles. Examples of statistical analysis of both these variables, and their presentation in the form of tables, diagrams and histograms are given.

Adolescent↗
PubMed2000

Ion exchange-based preconcentration for the determination of anions by capillary electrophoresis.

In the present paper a capillary zone electrophoresis (CZE)-compatible preconcentration technique for anions, based on ion exchange, is described. The described preconcentration approach has found limited use until recently because of the inherent elution step that leads to contamination of the sample with eluent components. In this paper, we describe an improved anion exchange-based preconcentration technique in which contamination of the sample with the eluent constituents, which occurs during anion elution from the preconcentration column, is eliminated by on-line chemical suppression on a packed-bed suppressor column. In the present communication, the basic principles of the proposed anion enrichment system are presented. The system was optimized, resulting in a minimal additional dilution of the eluted sample plug. This was achieved by the use of a computer-controlled, sensing/switching system. The effectiveness of the developed method was later tested on the determination of some anions in a synthetic sample using CE apparatus.

Anion Exchange Resins↗
PubMed1997

[Changes in body equilibrium response caused by breathing. A posturographic study with visual feedback].

BACKGROUND: Therapeutical methods involving holistic medicine are of increasing interest. The present study deals with the psychophysical breath work by Middendorf and examines whether it has an effect on reactions of the body's equilibrium system. METHODS: Different optical patterns were projected on a video screen to the test subject standing on a modified posturographic platform. Subjects were instructed to shift their center of gravity according to the patterns projected on the video screen. The patterns consisted of a line that had to be followed in the anterior-posterior and in the lateral plane, and of a circle which had to be followed clockwise and counterclockwise. PATIENTS: Three groups each consisting of 17 healthy persons were tested; group 1: advanced in breath training, group 2: beginners in breath training, group 3: no experience in breath work at all. RESULTS: Group 1 und 2 show significantly better results in the posturographic test with visual feedback than subjects without experience in breast work (group 3). Furthermore, posturographic results immediately after one hour of breath work reveal clear improvements in the body equilibrium. CONCLUSIONS: Psychophysical breath work by Middendorf leads to a general improvement of the body equilibrium which is stable over time. The positive results of this study lead to the assumption that breath work by Middendorf is a valuable method for treatment and rehabilitation of balance disorders.

Adult↗
PubMed2002

Root system architecture determines fitness in an Arabidopsis mutant in competition for immobile phosphate ions but not for nitrate ions.

Plant root systems often have complex branching patterns. Models indicate that a complex architecture is only required for the acquisition of immobile resources, such as phosphate; mobile ions, notably nitrate, can be effectively taken up by very restricted root systems. We have tested this prediction using the axr4 mutation of Arabidopsis thaliana, the principal phenotypic effect of which is to reduce the number of lateral roots. Arabidopsis thaliana is not a host for mycorrhizal fungi and so acquires all its nutrients through the root system. In both a pot experiment and a field experiment conducted under natural conditions for A. thaliana, we found that only phosphate, and not nitrate, affected the fitness of the mutant relative to the isogenic wild-type line, Columbia. These results confirm model predictions and have implications both for the evolution of complex root systems and for the design of efficient root systems for crops.

Arabidopsis↗
PubMed2002

[A morphologic evaluation of the sacroiliac joint and plate fixation on a pelvic model using a S1 pedicular screw, transiliosacral screws, and a compression rod for sacroiliac joint injuries].

OBJECTIVES: Morphological measurements were performed, of the articular surfaces and adjacent bone structures of the sacroiliac joint on dry bone specimens to determine the projection of the sacroiliac joint on the outer table of the posterior ilium. In addition, the effect of plate fixation using transiliosacral screws and a pedicular screw on S1 attached via a compression rod was evaluated on pelvic models to be applied in sacroiliac joint injuries. METHODS: Quantitative caliper measurements of dry bone specimens including 20 os coxae and 10 sacrum were made on the articular surfaces of the sacrum and the posterior ilium, thickness of the posterior iliac bone at different levels, and the distance from the outer walls of S1 and S2 foramina to the sacral facies articularis. After the construction of a plate matching the projection of the lateral sacral mass on the outer table of the posterior ilium, four transiliosacral screws were applied lateral to the sacral foramina on pelvic models. A pedicular screw sent to S1 was attached to the plate with a threaded compression rod. RESULTS: The mean values for the articular surface of (i) the posterior ilium were 53.3 mm (base length), 38.5 mm (height), and 56.2 mm (the distance from the anterior margin of the articular surface to the spina iliaca posterior superior); and (ii) the sacrum, 57.2 mm (base length), and 34.6 mm (height). The mean thickness of the posterior ilium was 19.2 mm, and the mean distance from the lateral walls of the sacral foramina at S1 and S2 levels to the articular surface was 21.7 mm. For the deduced projection, the perpendicular line from the middle of the base was found to be the safe zone for screw applications. CONCLUSION: Through a plate applied matching the projection area, multiple screws may be sent lateral to S1 and S2 foraminal levels without damage to the sacral neural and surrounding vital structures. A stable fixation can be achieved by combining the plate/screw system with a S1 pedicular screw.

Bone Plates↗