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PubMed2003

Effects of three PBDEs on development, reproduction and population growth rate of the harpacticoid copepod Nitocra spinipes.

The current knowledge concerning effects of polybrominated diphenyl ethers (PBDEs) on aquatic organisms is very limited. A full life-cycle (< or =26 days exposure) ecotoxicity test with the particle-feeding copepod Nitocra spinipes was therefore used to study effects of BDE-47, -99 and -100 on larval development rate (LDR) and population growth rate (r(m)). LDR significantly decreased in copepods exposed for 6 days to nominal concentrations > or =0.013 mg/l BDE-47 and > or =0.03 mg/l BDE-99. Large concentration ratios (< or =338) between adult acute and juvenile subchronic endpoints were observed. Exposure over the full life cycle (< or =26 days) showed that r(m) in general was a less sensitive endpoint than LDR. Still, the r(m) in copepods exposed to 0.04 mg/l BDE-47 was significantly reduced compared to the controls (***P<0.001). Partitioning experiments with 14C-BDE-47 and 14C-BDE-99 in the test system showed that the major fractions (approximately 50-80%) were associated to particulate material. Our findings indicate that development and reproduction in N. spinipes are sensitive to the tested PBDEs and that ingestion of particle-adsorbed PBDEs most likely is the predominant route of exposure in N. spinipes. However, to further improve the usefulness of laboratory effect levels of PBDEs and other lipophilic substances for environmental risk assessment, it is important to develop ecotoxicological tools, which can evaluate and rate the toxic contribution from different matrices, such as suspended particles, sediment, food, water etc.

Animals↗
PubMed2005

The Caenorhabditis elegans aristaless orthologue, alr-1, is required for maintaining the functional and structural integrity of the amphid sensory organs.

The homeobox-containing aristaless-related protein ARX has been directly linked to the development of a number of human disorders involving mental retardation and epilepsy and clearly plays a critical role in development of the vertebrate central nervous system. In this work, we investigate the role of ALR-1, the Caenorhabditis elegans aristaless orthologue, in amphid sensory function. Our studies indicate that ALR-1 is required for maintenance of the amphid organ structure throughout larval development. Mutant analysis indicates a progressive loss in the amphid neurons' ability to fill with lipophilic dyes as well as a declining chemotactic response. The degeneration in amphid function corresponds with a failure of the glial-like amphid socket cell to maintain its specific cell shape and cell-cell contacts. Consistent with ALR-1 expression within the amphid socket cell, our results indicate a cell autonomous role for ALR-1 in maintaining cell shape. Furthermore, we demonstrate a role for ALR-1 in the proper morphogenesis of the anterior hypodermis. Genetic interaction tests also suggest that ALR-1 may function cooperatively with the cell adhesion processes in maintaining the amphid sensory organs.

Animals↗
PubMed1999

Posthatching development of spinal motoneurons in the angelfish Pterophyllum scalare.

We investigated the posthatching developmental sequence of spinal motoneurons innervating the axial muscles in the teleost angelfish, Pterophyllum scalare, by means of retrograde labeling with horseradish peroxidase. Two discrete types of spinal motoneurons, primary-type motoneurons and secondary motoneurons were labeled in a temporally different sequence during the course of larval development. These two types of motoneurons were morphologically distinguishable from one other. Primary-type motoneurons are generated by day 1 posthatching and do not increase in number over the observed period (to day 12 posthatching). In contrast, the secondary motoneurons increase in number through posthatching day 3. Differentiation of the spinal motoneurons appears to be nearly complete a few days before the onset of free swimming. In addition, the data suggest that the differentiation of secondary motoneurons precedes the development of the red muscle that is to be innervated by the motoneurons.

Animals↗
PubMed1984

In vitro development of third- and fourth-stage larvae of Dirofilaria immitis: comparison of basal culture media, serum levels and possible serum substitutes.

In vitro development and survival of third-stage larvae of Dirofilaria immitis were compared in four different culture media and in the presence of varying concentrations of four different medium supplements. Motility and the incidence of third- to fourth-stage molting were used as criteria for evaluating different culture conditions. No significant differences in either motility or molting response were detected between larvae cultured in NCTC-135, F12(K), CMRL 1066 or Dulbecco's Modified Eagle's Medium. Fetal calf serum enhanced development and survival of the cultured larvae in dose-dependent fashion. Its effects were maximal at a concentration of 20 percent of the total medium volume. Addition of a commercial medium supplement, NuSerum, also gave a dose-related increase in larval development and viability. The activity of NuSerum in this respect was comparable to that of fetal calf serum. The tripeptide glycylhistidyllysine and bovine serum albumin, fraction V both failed to stimulate development of third-stage D. immitis larvae in vitro.

Animals↗
PubMed1980

The initiation of pupariation in Drosophila: dependence on growth of the imaginal discs.

Regeneration was induced in the imaginal discs in situ following lesions caused by heat-sensitive cell-lethal mutations. A clonal analysis of this event demonstrated that the subsequent delay in pupariation was correlated with the amount of extra growth that occurred during the regeneration. Pupariation of heat-treated gynandromorphs bearing the mutations was also retarded, and the duration of larval development increased with greater amounts of mutant tissue, it was therefore correlated with the extent of the lesions in the imaginal discs. Elimination of entire imaginal discs, or the presence of very small amounts of lethal tissue, did not result in prolonged larval life.

Animals↗
PubMed2005

Hybridization between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae): development and morphological characterization of F1 hybrids.

Reciprocal hybridizations between Helicoverpa armigera (Hübner) and Helicoverpa assulta (Guenée) were studied. The cross between females of H. armigera and males of H. assulta yielded only fertile males and sterile individuals lacking an aedeagus, valva or ostium bursae. A total of 492 larvae of the F1 generation were obtained and 374 of these completed larval development and pupated. Only 203 pupae were morphologically normal males, the remaining 171 pupae were malformed. Larvae and pupae that gave rise to morphologically abnormal adults exhibited longer development times. Sterility was not only associated with malformed external sex organs, but also a range of abnormalities of the internal reproductive system: (i) loss of internal reproductive organs, (ii) with one to three copies of an undeveloped bursa copulatrix; or (iii) with one or two undeveloped testes. Normal male hybrid adults showed higher flight activity in comparison with males of both species. In contrast, the cross between females of H. assulta and males of H. armigera yielded morphologically normal offspring (80 males and 83 females). The interaction of the Z-chromosome from H. assulta with autosomes from H. armigera might result in morphological abnormalities found in hybrids and backcrosses, and maternal-zygotic incompatibilities might contribute to sex bias attributed to hybrid inviability.

Animals↗
PubMed2002

Tail morphogenesis in the ascidian, Ciona intestinalis, requires cooperation between notochord and muscle.

We present evidence that notochord and muscle differentiation are crucial for morphogenesis of the ascidian tail. We developed a novel approach for embryological manipulation of the developing larval tissues using a simple method to introduce DNA into Ciona intestinalis and the several available tissue-specific promoters. With such promoters, we misexpressed the Xenopus homeobox gene bix in notochord or muscle of Ciona embryos as a means of interfering with development of these tissues. Ciona embryos expressing bix in the notochord from the 64-cell stage develop into larvae with very short tails, in which the notochord precursors fail to intercalate and differentiate. Larvae with mosaic expression of bix have intermediate phenotypes, in which a partial notochord is formed by the precursor cells that did not receive the transgene while the precursors that express the transgene cluster together and fail to undergo any of the cell-shape changes associated with notochord differentiation. Muscle cells adjacent to differentiated notochord cells are properly patterned, while those next to the notochord precursor cells transformed by bix exhibit various patterning defects. In these embryos, the neural tube extends in the tail to form a nerve cord, while the endodermal strand fails to enter the tail region. Similarly, expression of bix in muscle progenitors impairs differentiation of muscle cells, and as a result, notochord cells fail to undergo normal extension movements. Hence, these larvae have a shorter tail, due to a block in the elongation of the notochord. Taken together, these observations suggest that tail formation in ascidian larvae requires not only signaling from notochord to muscle cells, but also a "retrograde" signal from muscle cells to notochord.

Animals↗
PubMed1983

Differential mRNA accumulation and translation during Spisula development.

The patterns of proteins synthesized in developing Spisula embryos and larvae were compared with in vitro translation products by one-dimensional gel electrophoresis. Major changes in the in vivo pattern occur at fertilization; these are regulated at the translational level (Rosenthal, Hunt, and Ruderman, 1980, Cell 20, 487-494). The pattern is further altered by midcleavage, and subsequent development is accompanied by frequent changes in the kinds of proteins made. By midcleavage many of the in vivo changes are paralleled by alterations in mRNA levels. Three cDNA clones containing developmentally regulated, nonmitochondrial sequences were isolated from a library constructed from veliger larval RNA. Clone 3v4 encodes alpha-tubulin. Clone 12v4 encodes a 35,000-D protein of unknown function. The protein product of clone 10v8 has not been identified. The concentration of alpha-tubulin RNA is relatively low through midcleavage, increases by the swimming gastrula stage, and is maintained at a moderately high level throughout larval development. 10v8 and 12v4 RNAs first appear in trochophore larvae; their concentrations peak 10-12 hr later, and then decline. The proportions of alpha-tubulin and 10v8 RNA that are translated vary with developmental stage. During early cleavage very little alpha-tubulin RNA is on polysomes; in swimming gastrulae 64% of this mRNA is polysomal. Seventy percent of 10v8 RNA is translated in the trochophore larva, while only approximately 40% is polysomal in the 21-hr veliger. These results show that translational regulation may be superimposed on changes in cytoplasmic mRNA concentrations to determine the level of gene expression during embryogenesis.

Animals↗
PubMed1995

Differences in the decrease in regenerative capacity of various brain regions of Xenopus laevis are related to differences in the undifferentiated cell populations.

The extent of the undifferentiated cell population in normal and regenerating brains of larvae and metamorphosed individuals of Xenopus laevis has been analyzed by means of an immunocytochemical method and mitotic index determinations. Results show that the decrease in regenerative capacity of the brain during larval development and after metamorphosis is in relation with the gradual reduction of the population of undifferentiated cells and that the different regenerative capacities of the various brain districts are related to quantitative and qualitative differences in this cell population. While in the early larval stages the cell population formed of actively cycling cells is very large and widespread, in late larval stages and after metamorphosis these cells localize in some encephalic areas (matrix zones). This localization occurs later in the telencephalon than in the rhombencephalon and in mesencephalon. The less conspicuous decrement in the regenerative capacity of the telencephalon than of other encephalic districts of froglets, particularly the mesencephalon, is related to the presence of a larger number of actively cycling cells together with a rather large number of undifferentiated cells which are in a temporary quiescent state from which they may re-enter the actively cycling state in response to proliferation promoting factors.

Animals↗
PubMed1983

Differential expression of two abundant messenger RNAs during development of Sarcophaga peregrina.

Expression of two abundant mRNAs in the fat body of third instar larvae of Sarcophaga peregrina was studied using genomic clones of storage protein and 25K protein as probes. It was found that these two genes were expressed sequentially in the third instar with a 20-hr interval between their expressions. No amplification of genes was detected during larval development, suggesting that the high level of mRNA was due to efficient transcription. The contents of the two mRNAs in third instar larvae were about the same, although the synthesis of storage protein was much more than that of 25K protein.

Adipose Tissue↗
PubMed2004

Yantar, a conserved arginine-rich protein is involved in Drosophila hemocyte development.

To identify novel factors involved in Drosophila hematopoiesis, we screened a collection of lethal recessive mutations that also affected normal hemocyte composition in larvae. We present the characterization of the gene yantar (ytr) for which we isolated null and hypomorphic mutations that were associated with severe defects in hemocyte differentiation and proliferation; ytr is predominantly expressed in the hematopoietic tissue during larval development and encodes an evolutionary conserved protein which is predominantly localized in the nucleus. The hematopoietic phenotype in ytr mutants is consistent with a defect or block in differentiation of precursor hemocytes: mutant larvae have enlarged lymph glands (LGs) and have an excess of circulating hemocytes. In addition, many cells exhibit both lamellocyte and crystal cell markers. Ytr function has been preserved in evolution as hematopoietic specific expression of the Drosophila or mouse Ytr proteins rescue the differentiation defects in mutant hemocytes.

3T3 Cells↗
PubMed1999

Muscle growth and development in Atlantic cod larvae (Gadus morhua L.), related to different somatic growth rates.

The present study describes the development of the axial musculature in first-feeding larvae of Atlantic cod (Gadus morhua L.) with different somatic growth rates achieved by using different nutritional conditions. Muscle growth was assessed by determining the number of muscle fibres (hyperplasia) and the growth of existing fibres (hypertrophy). Larvae were fed rotifers containing a high (1. 4; treatment 1) or low (0.2; treatment 2) ratio of docosahexaenoic acid to eicosapentaenoic acid from day 5 after hatching. From day 17, the larvae were fed Artemia nauplii with the same enrichment in both treatments. Treatment 1 gave the highest somatic growth rate and hence the highest dry mass at the end of the experiment, but no difference in larval standard length was found between treatments. In slow-growing larvae, higher priority was thus put into reaching a certain length than into increasing muscle mass. The largest fibres, which were present from hatching, increased in cross-sectional area during larval development, but no differences were found between treatments in the cross-sectional area of individual fibres or the total cross-sectional area of these fibres at the end of the experiment. The first white recruitment fibres were observed at the dorsal and ventral apices of the myotome at approximately the onset of first feeding (larval length 4.5 mm). In larvae 8.5 mm long, the total cross-sectional area of white muscle fibres in the treatment 2 group was 75 % of that in the treatment 1 group. The highest somatic growth rate was associated with an increased contribution of hyperplasia to axial white muscle growth. In the faster-growing larval group, the relative contribution of hyperplasia to the total white muscle cross-sectional area was 50 %, whereas it was 41 % in the slower-growing larval group. The subsequent growth potential may thus be negatively affected by inadequate larval feeding.

Animals↗
PubMed2006

Duplicate sfrp1 genes in zebrafish: sfrp1a is dynamically expressed in the developing central nervous system, gut and lateral line.

The secreted frizzled-related proteins (Sfrp) are a family of soluble proteins with diverse biological functions having the capacity to bind Wnt ligands, to modulate Wnt signalling, and to signal directly via the Wnt receptor, Frizzled. In an enhancer trap screen for embryonic expression in zebrafish we identified an sfrp1 gene. Previous studies suggest an important role for sfrp1 in eye development, however, no data have been reported using the zebrafish model. In this paper, we describe duplicate sfrp1 genes in zebrafish and present a detailed analysis of the expression profile of both genes. Whole mount in situ hybridisation analyses of sfrp1a during embryonic and larval development revealed a dynamic expression profile, including: the central nervous system, where sfrp1a was regionally expressed throughout the brain and developing eye; the posterior gut, from the time of endodermal cell condensation; the lateral line, where sfrp1a was expressed in the migrating primordia and interneuromast cells that give rise to the sensory organs. Other sites included the blastoderm, segmenting mesoderm, olfactory placode, developing ear, pronephros and fin-bud. We have also analysed sfrp1b expression during embryonic development. Surprisingly this gene exhibited a divergent expression profile being limited to the yolk syncytium under the elongating tail-bud, which later covered the distal yolk extension, and transiently in the tail-bud mesenchyme. Overall, our studies provide a basis for future analyses of these developmentally important factors using the zebrafish model.

Amino Acid Sequence↗
PubMed2005

No evidence of androgenic hormone from the testes of the glowworm, Lampyris noctiluca.

The widely accepted concept, stating that insects have no true sex hormones, and that primary as well as secondary sex characteristics are controlled by the genetic inventory of each single cell, is challenged by the report of Naisse, J. [1966a. Contrôle endocrinien de la différenciation sexuelle chez l'Insecte Lampyris noctiluca (Coléoptère Malacoderme Lampyride). I. Rôle androgène des testicules, Arch. Biol. Liège, 77, 139-201] on the discovery of an androgenic hormone in the glowworm, Lampyris noctiluca. This case is of special interest, since it may point to an ancestral mode of sex differentiation in arthropods, considering that androgenic hormones have been discovered and characterized in crustaceans. With the intention to further characterize the androgenic hormone in the glowworm, and to establish a bioassay, we tried to repeat Naisses's transplantation experiments, according to which, androgen producing testes implanted into female larvae should masculinize the female's gonads and all other female features of the sexually strongly dimorphic pupae and beetles. We found, however, that larval development of the glowworm proceeded differently than reported by Naisse, and that sexing of larvae was not possible. Therefore, "blind" transplantations had to be performed. The results of our experiments showed, however, unequivocally, that an androgenic hormone, allegedly synthesized by the apical tissue of larval testes, was not involved in sex differentiation. We found, that in transplants, where testes and ovaries were even located closely to each other, both matured and formed spermatozoa in the testes and vitellogenic oocytes in the ovaries. Masculinization of ovaries was never observed, and the sex of the recipient was always in accordance with the sex of its own gonads. We therefore conclude that Lampyris noctiluca does not synthesize an androgenic hormone in the larval testes, and that sex differentiation is probably regulated as in other insect species. (The apical testis tissue of the glowworm was previously shown to represent progenitors of spermatogonial cyst cells [Balles, S., Maas, U., Sehn, E., Dorn, A., 2002. Testis differentiation in the glowworm, Lampyris noctiluca, with special reference to the apical tissue. J. Morphol. 251, 22-37].).

Androgens↗
PubMed2002

Epizootiology of Hyalinocysta chapmani (Microsporidia: Thelohaniidae) infections in field populations of Culiseta melanura (Diptera: Culicidae) and Orthocyclops modestus (Copepoda: Cyclopidae): a three-year investigation.

The epizootiology, transmission dynamics and survival strategies employed by the microsporidium Hyalinocysta chapmani were examined in field populations of its primary mosquito host, Culiseta melanura and its intermediate copepod host, Orthocyclops modestus over a three-year period in an aquatic subterranean habitat. H. chapmani was enzootic and was maintained in a continuous cycle of horizontal transmission between each host. There were three distinct periods during the summer and fall when developing mosquito larvae acquired infections; each was preceded by or coincident with the detection of infected copepods. Results were corroborated in laboratory bioassays, wherein transmission was achieved in mosquito larvae that were reared in water and sediment samples taken from the site during the same time periods. The highest infection rates, ranging from 60% to 48%, were repeatedly observed during the first six weeks of larval development. These were coincident with the most sustained collections of infected copepods obtained during the year and highest levels of infection achieved in the laboratory transmission studies. The high prevalence rates of lethal infection observed in larval populations of C. melanura at this site are among the highest recorded for any mosquito-parasitic microsporidium and clearly suggest that H. chapmani is an important natural enemy of C. melanura. H. chapmani appears to overwinter in diapausing mosquito larvae but may also persist in copepods. The absence of vertical transmission in the life cycle of H. chapmani and the sole reliance on horizontal transmission via an intermediate host are unique survival strategies not seen among other mosquito-parasitic microsporidia. The epizootiological data suggest that this transmission strategy is a function of the biological attributes of the hosts and the comparatively stable environment in which they inhabit. The subterranean habitat is inundated with water throughout the year; copepods are omnipresent and C. melanura has overlapping broods. The spatial and temporal overlap of both hosts affords abundant opportunity for continuous horizontal transmission and increases the likelihood that H. chapmani will find a target host. It is hypothesized that natural selection has favored the production of meiospores in female host mosquitoes rather than congenital transfer of infection to progeny via ovarian infection as a strategy for achieving greater transmission success.

Animals↗
PubMed2001

Immunohistochemical distribution of cytochrome P4501A in larvae and fingerlings of the Siberian sturgeon, Acipenser baeri.

In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPIA), from hatching until 30 days posthatching in developing Siberian sturgeon, Acipenser baeri. For this purpose, a polyclonal (BN-1) antiserum developed against a conservative sequence of piscine CYP1A and a monoclonal (C10-7) antiserum directed against cod CYP1A were used on paraffin-embedded samples. From hatching onwards, distinct CYP1A immunoreactivity was distinctly observed in the following tissues and cells: envelope of oil droplets, matrix and syncytium of the yolk-sac, sinusoids, biliary epithelial cells and hepatocytes. In the digestive tract, buccopharyngeal, oesophageal, gastric and intestinal epithelia, as well as the cytoplasm and brush border of enterocytes were CYP1A-positive. Interestingly, gastric glands and melanin-plug present within lumen of the digestive system were strongly immunoreactive. Kidney (epithelia of renal tubules), gills (pillar and endothelial cells), skin (epithelial cells), muscle fibres of heart and eye (retina) were positive. In brain, we observed a strong CYP1A staining in the developing telencephalon and especially in olfactory system, as well as in those nerve fibres running ventrally toward the posterior brain. A strong CYP1A staining was observed in vascular endothelia of all organs/tissues, especially in the liver. In general, the intensity of CYP1A immunostaining increased during larval development, suggesting besides its known metabolic function (endogenous and/or exogenous), a possible participation of this heme-protein in control of cell division, regulation of growth and differentiation.

Animals↗
PubMed2006

Realized correlated responses to artificial selection on pre-adult life-history traits in a butterfly.

We use artificial selection experiments targeted on egg size, development time or pupal mass within a single butterfly population followed by a common-garden experiment to explore the interactions among these life-history traits. Relationships were predicted to be negative between egg size and development time, but to be positive between development time and body size and between egg size and body size. Correlated responses to selection were in part inconsistent with these predictions. Although there was evidence for a positive genetic correlation between egg and body size, there was no support for genetic correlations between larval development time and either egg size or pupal mass. Phenotypic correlations among the three target traits of selection gave comparable results for the relationships between egg mass and development time (no association) as well as between egg mass and pupal mass (positive association), but not for the relation between development time and pupal mass (negative phenotypic correlation). In summary, correlated responses to selection as well as phenotypic correlations were rather unpredictable. The impact of variation in acquisition and allocation of energy as well as of the benign conditions used deserve further investigation.

Animals↗
PubMedDate not supplied

[Nematodes of the genus Spirura, parasites of Tupaia and Nycticebus in Malaysia (author's transl)].

Morphological study of two Spirura parasites of the oesophageal and the gastric wall of Tupaia and Nycticebus in Malaysia. -- Spirura malayensis n. sp. is found both in Tupaia in the District of Selangor (West Malaysia) and in Nycticebus coucang in Borneo. Its very primitive characteristics relate it to S. diplocyphos Chabaud, Brygoo and Petter, 1965, parasite of lemurs from Madagascar. Its larval development was obtained experimentally in Blatella germanica. -- Spirura aurangabadensis (Ali and Lovekar, 1966) described from a microchiroptera in India is found in west Malaysia in a Nycticebus coucang, and in a Tupaia glis. -- The distribution of the different species and the comparative study of the larval and adult cephalic structures show that the genus Spirura arose and became diversified in the old world in very primitive hosts according to two main evolutive lines.

Animals↗