[Monitoring of artificial insemination].
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Three hundred young Dutch Friesian and Holstein-Friesian bulls, kept in the Central Opfokstation (Central Breeding Station) in Terwispel (Friesland), were studied cytogenetically, using conventional staining methods. Structural chromosome aberrations were not observed. Four animals showed XX/XY-chimerism in the lymphocytes, probably caused by the interchange of haemopoietic cells between the male and its female co-twin by placental vascular anastomosis. The number of (iso)chromatid gaps in 25 metaphase plates varied from 0 to 4. Chromosome breaks were not observed. The chimeric bulls and those with (iso)chromatid gaps were not found to show significantly reduced fertility rates.
A total of 218 goats were inseminated with thawed frozen semen and a non-surgical intra-uterine insemination technique. Of the various groups inseminated the percentage Boergoat does kidding was 60.8% (46) and 70.9% (31), the percentage Angoragoat does kidding was 62.3% (53), 61.8% (68) and 60.0% (20). A dose of 75 X 10(6) sperm/ml was found to be suitable for intra-uterine insemination of Angora does.
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Embryos were collected from repeat breeder heifers and virgin heifers seven days after insemination, classified and transferred to the uterine horn contralateral to the corpus luteum of synchronised inseminated recipients. Altogether 35 transfers were performed, all reciprocally between repeat breeder heifers and virgin heifers. The recipients were slaughtered either 16 to 17 days or 32 to 35 days after insemination. The survival rate of the native embryos was lower among the repeat breeder heifers than among the virgin heifers both at 16 to 17 days (six of nine vs six of six) and at 32 to 35 days (three of 10 vs seven of 10) after insemination. A higher proportion of embryos transferred from repeat breeder heifers to virgin heifers than from virgin heifers to repeat breeder heifers survived to days 16 to 17 (five of six vs two of nine), while the same proportion of embryos survived to days 32 to 35 (two of 10) in both heifer categories. The results suggest that the uterine environment in repeat breeder heifers is suboptimal for the support of normal embryonic development.
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