Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “GANGLIA, SPINAL”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,081 records · Page 60Linked to original sources
PubMed1995

Sympathetic and afferent neurones projecting into forelimb and trunk nerves and the anatomical organization of the thoracic sympathetic outflow of the rat.

The anatomy of the cervicothoracic sympathetic nerves was studied in the rat. Details of the arrangements of white and grey rami communicantes and superior cervical, middle cervical and stellate ganglia are given. Dorsal root and sympathetic ganglion neurones projecting to skin and muscle of the forelimb and trunk were labelled retrogradely with horseradish peroxidase (HRP) in order to study their number, segmental distribution and location. HRP was applied to forelimb nerves supplying skeletal muscles (Ramus profundus of radial nerve, RP) or hairy skin (N. cutaneus brachii lateralis superior of axillary nerve, CB), to mixed nerves (median nerve, ME; ulnar nerve, UL; radial nerve, RA) and to segmental thoracic nerves supplying hairy skin of the back (dorsal cutaneous nerve, CD) and to mixed internal intercostal nerves (IC). All sensory and sympathetic neurones were located ipsilaterally. In the forelimb nerves sensory somata were commonly restricted to two or three adjacent dorsal root ganglia (usually C6-7 for CB; C7-8 for ME; C7-Th1 for RA and RP; C8-Th1 for UL). Nearly all of the sympathetic somata were located in the middle cervical and stellate ganglia (fusion of C6-Th3). Some 0-0.4% lay in Th4 and Th5, none in the superior cervical ganglia. In the trunk nerves sensory somata were strictly segmentally organized. Sympathetic somata were distributed more widely over 4-5 segments with 50-55% in the segmental ganglion and up to 41% in the next caudal segment. From the data, it is estimated that 400 sympathetic (28%) and 1050 afferent neurones project into CB, 1660 (29%) and 4050 into RA, 540 (42%) and 760 into RP, 1010 (22%) and 3670 into ME, 880 (22%) and 3040 into UL, 350 (25%) and 1040 into IC and 500 (27%) and 1370 into CD.

Animals↗
PubMed1994

Projections from the prevertebral and major pelvic ganglia to the ileum and large intestine of the male rat.

The sympathetic innervation of the gut arises from the prevertebral and pelvic ganglia, and to a variable extent, from neurons located in the paravertebral ganglia, the splanchnic and intermesenteric nerves. In this study we have injected retrograde tracers into the wall of the ileum and several regions of the large intestine to determine the proportion of neurons supplying these regions from each of the ganglia and nerves. The sympathetic supply to the ileum arises primarily from neurons in the splanchnic nerves and the paravertebral ganglia T9-T11 (SPL) and the coeliaco-mesenteric ganglion complex (CG/SMG), with a small supply from the proximal intermesenteric nerves (IMN). The distribution of neurons projecting to the proximal colon is very similar, although some labelled neurons are found throughout the length of the IMN and also in the inferior mesenteric ganglion (IMG). The middle colon is primarily supplied by the IMN and the IMG, although this region receives innervation from all the ganglia and nerves investigated, including the major pelvic ganglia (MPG). Neurons located in the distal two-thirds on the IMN and more caudal structures were labelled from the distal colon. The distal colon is the only region of gut to receive a relatively large innervation from the MPG, with approximately one third of labelled neurons in each of the IMN, IMG and the MPG. These studies also compared two retrograde tracers, Fast Blue and Fluorogold. Fluorogold was found specifically to label neurons projecting to each region of intestine injected. However, when injected into the middle colon, Fast Blue labelled neurons that project to this region as well as many neurons that Fluorogold studies indicate project to other regions; Fast Blue should therefore be used only with caution.

Amidines↗
PubMed1981

Requirements for growth of neurites from embryonic chick cerebellum in culture.

Embryonic chick cerebella were cut into pieces and cultured under various conditions. Neurites were extended actively to collagen gel for a week or so and then degenerated. The neurite growth for the first two days was similar, also in the serum-free medium. Among 15 substances tested, insulin and cyclic AMP facilitated the growth.

Animals↗
PubMed1981

Trophic activities for dorsal root and sympathetic ganglionic neurons in media conditioned by Schwann and other peripheral cells.

We describe here the use of 3 established nerve growth factor (NGF) targets (cultured neurons from dissociated chick embryo sympathetic ganglia, and chick embryo or neonatal mouse dorsal root ganglia) to investigate neuronotrophic activities in conditioned media (CMs) from: (i) chick embryo heart; (ii) purified mouse Schwann cells; and (iii) clonal rat Schwannoma RN22 cells. In chick sympathetic and mouse dorsal root ganglionic cultures, all 3 CMs supported survival of the same number of neurons as did mouse submaxillary NGF, and in most cases no increased survival resulted from concurrent administration of NGF and any one CM. NGF and CM activities were quantitated in each of the responsive cell systems. No differences were seen when either test population was used for the same agent, or when different CMs were examined on the same test cells. The CM activity, unlike that of NGF, was not blocked by even excess amounts of antiserum against mouse submaxillary NGF. The neuronotrophic activity of CMs appears to reside with macromolecular constituents. None of the CMs displayed trophic activity on chick embryo dorsal root ganglionic neurons. However, at least one of them (RN22 medium) had drastic effects on these ganglionic cells even in the presence of NGF, leaving open the possibility of a 'toxic' factor overriding putative trophic agents.

Animals↗
PubMed1983

Postnatal changes in the activity of glutamate dehydrogenase and aspartate aminotransferase in the rat nervous system with special reference to the glutamate transmitter metabolism.

The activities of aspartate aminotransferase (AAT) and glutamate dehydrogenase (GIDH), the major glutamate metabolizing enzymes, were studied in hippocampal formation, cerebellar cortex, dorsal root ganglia, superior cervical ganglia and liver as a function of postnatal development. At birth, in all these nervous tissues the enzyme activities were quite low and showed similar levels (AAT 7-15 U/g wet weight; 0.18-0.23 U/mg protein; GIDH 3.4-13 U/g wet weight; 0.07-0.18 U/mg protein). Based on protein, AAT activity increased during the postnatal period studied 5.8 and 3.8 times in the hippocampal formation and cerebellar cortex, respectively, while the respective GIDH rise was 5.2 and 2.3 times. During postnatal maturation, enzyme activities in dorsal root ganglia showed only minor changes. In superior cervical ganglia, AAT and GIDH were remarkably constant. In liver the enzyme activities changed during postnatal development, but the activity curve profile was quite distinct from those obtained for brain regions. The steep rise of AAT and GIDH activities in brain regions is discussed as being a consequence of the maturation of preferably glutamatergic structures. Glutamatergic transmission processes obviously do not take place in superior cervical ganglia and dorsal root ganglia, and certainly not in liver. The present results suggest a quantitatively significant participation of glutamate transmitter metabolism in proportion to the whole glutamate metabolism of the CNS.

Aging↗
PubMed1984

Expression of glial antigens C1 and M1 in the peripheral nervous system during development and regeneration.

The expression of C1 and M1 antigens was studied by indirect immunofluorescence methods in histological sections of peripheral nerves and ganglia of C57BL/6J mice during development and regeneration. In sciatic nerves of adult mice, C1 but not M1 antigen is found in vimentin- and glial fibrillary acidic protein (GFAP)-positive Schwann cells. A similar distribution is also seen in trigeminal nerve, dorsal root and superior cervical ganglia, and olfactory nerve. In all cases vimentin-positive structures outnumber GFAP- or C1 antigen-positive ones. At birth, C1 antigen and vimentin are expressed in sciatic nerves, but GFAP is not yet detectable. M1 antigen cannot be detected in Schwann cells. In monolayer cultures of neonatal mouse dorsal root ganglia, C1 antigen is expressed in a fibrillary staining pattern in some, but not all morphologically identified Schwann cells. In vitro, M1 antigen is not detectable in Schwann cells. After lesioning sciatic nerves of adult mice by cut or crush, detectable levels of C1 antigen rise after 4-6 days: The number of immunofluorescently labeled structures and their relative intensities are drastically augmented, first distally more so than proximally, over control values from non-lesioned, i.e. contralateral nerves. A similar augmentation is also observed for vimentin and GFAP. M1 antigen expression does not reach detectable levels in Schwann cells under these conditions. The increased detectability of C1 antigen persists up to 150 days after lesioning, the longest time period tested.

Animals↗
PubMed1990

1,1,3 tricyano-2-amino-1-propene (Triap): a small molecule which mimics or potentiates nerve growth factor.

1,1,3 Tricyano-2-amino-1-propene (Triap) is a small molecular weight compound which increases the rate of nerve and tissue regeneration in several experimental systems. Early experiments with this compound showed that, like nerve growth factor (NGF), Triap induced neurite formation in chick spinal ganglia. To assess the similarity between NGF and Triap, we compared the effects of Triap and NGF on a rat pheochromocytoma cell line (PC12) and on cell survival in a primary chick neuronal culture. In the latter, Triap at less than 0.01 nM preserved neurons and caused them to extend neurites as did 1 nM NGF. Triap induced neurite outgrowth in the PC12 cell line giving a maximal response (40-50% of the maximal response of NGF) at a concentration of 20 micrograms/ml (151 microM). Triap's morphological effects were not inhibited by antibodies directed against NGF or the NGF receptor. Low concentrations of Triap also potentiated the morphological effects of NGF. Triap induced an increase in cell-substratum adhesion and cellular hypertrophy in PC12 cells and also potentiated the adhesive actions of NGF. Triap had no effect on ornithine decarboxylase activity even though it potentiated NGF's effects on this enzyme. These data indicate that Triap induces neurotrophic effects and does not seem to act through the same mechanisms as NGF but can potentiate many of NGF's morphological and biochemical actions.

Animals↗
PubMed1993

Characterization of the neural crest defect in Splotch (Sp1H) mutant mice using a lacZ transgene.

We have reinvestigated the neural crest defect of Splotch (Sp1H) mutant embryos using the tissue specific expression of lacZ by the HCMV-IEP-lacZ (CMZ) transgene as a marker. The CMZ transgene was backcrossed onto the Sp1H mutant background, which has been shown to carry mutations in the Pax-3 gene. The CMZ transgene has previously been shown to be expressed in some neural crest-derived neural tissues of midgestation embryos. The pattern of CMZ expression in Splotch mutants is not caused by alterations of transgene transcription, but demonstrates morphological deviations of neural crest development. The gradual size reduction of spinal ganglia along a rostrocaudal gradient is shown to occur concomitantly with a size reduction of the sympathetic ganglia. CMZ expression also reveals the total absence of sympathetic ganglion cells in thoracic and lumbar segments of Sp1H homozygotes, which is confirmed in serial sections. Observations in whole mounts of CMZ transgenic homozygotes suggest that cranial nerve ganglia develop normally in these embryos. CMZ is expressed in epithelial cells around the neural tube defect in Splotch mutants at the epidermal/neuroepithelial boundary. It is proposed that this expression represents premigratory neural crest cells that remain within the epithelial layer around the neural tube defect. These observations are discussed with reference to the normal pattern of Pax-3 expression.

Animals↗
PubMed1995

Involvement of an axonal reflex in IgE-mediated inflammation in mouse skin.

A neurogenic component of IgE-mediated inflammation was demonstrated in mice by footpad denervation. Footpad swelling was reduced 26% following sciatic nerve transection, but unaffected by rhizotomy or spinal nerve transection. These data provide in vivo evidence that an axonal reflex is involved in IgE-mediated inflammation and completed distal to the cell bodies of the sensory neurons located in the lumbar spinal ganglia. Furthermore, depletion of neuropeptides with capsaicin also reduced IgE-mediated swelling by 26%, indicating that unmyelinated axons are involved in the neurogenic component of IgE-mediated inflammation.

Animals↗
PubMed1990

Expression of neurotrophic activity in Xenopus oocytes injected with mRNA from wounded rat cerebral cortex.

Injury to the cerebral cortex of the rat brain has been shown to induce the expression of neurotrophic factors for dissociated peripheral and central neurons in culture. We confirm this phenomenon and report that Xenopus laevis oocytes injected with mRNA extracted from wounded rat cortex expressed similar neurotrophic activity. To detect the low amounts of neurotrophic factors that could be expected from the oocyte translation system, a miniaturization of the assay for neurotrophic and cell-surviving activity was developed using Terasaki microtiter plates for culture of chicken embryo sympathetic ganglion cells. Messenger RNA (mRNA) was size-fractionated on a sucrose gradient and RNAs from each fraction were injected into oocytes. Neurotrophic activity was recovered from the homogenates and from the incubation media of oocytes injected with mRNA from 7 day post-lesion cortex. Messenger RNAs in the active fractions ranged in size from 0.8 to 1.8 kb. As much as 20% of the activity was secreted by the oocytes. No significant neurotrophic activity was detected from oocytes injected with mRNA fractions extracted from the cortex of control rats or from other gradient fractions from post-lesion cortex.

Animals↗
PubMed1992

Expression of the tissue specific splicing protein SmN in neuronal cell lines and in regions of the brain with different splicing capacities.

The SmN protein is closely related to the ubiquitously expressed SmB and B' RNA splicing proteins but is expressed in only a limited range of tissues and cell types. The expression of SmN in a range of neuronal and non-neuronal cell lines correlates with their ability to splice the calcitonin/CGRP transcript to produce the mRNA encoding CGRP rather than that encoding calcitonin. Moreover, the SmN mRNA shows a widespread distribution within the brain and spinal ganglia being present in neuronal cells in all regions which naturally produce CGRP as well as in those areas which do not naturally express the calcitonin/CGRP gene but which can correctly splice the CGRP mRNA in transgenic mice expressing the calcitonin/CGRP gene in all cell types. Interestingly however the mRNA encoding SmN is also found in a few areas of the brain which can only carry out calcitonin-specific splicing in transgenic mice, such as the Purkinje layer of the cerebellum and the inferior colliculus. The possible role of SmN in the regulation of splicing in neuronal cells is discussed in the light of these results.

Animals↗
PubMedDate not supplied

Coexistence of calcitonin gene-related peptide and galanin immunoreactivity in female rat pelvic and lumbosacral dorsal root ganglia.

Coexistence of immunoreactivity for calcitonin gene-related peptide (CGRP) and galanin (GAL) was examined in varicose nerve endings in female rat pelvic paracervical ganglia (PG) and in perikarya of lumbosacral dorsal root ganglia (DRG). Varicose peptide-containing nerves were closely adjacent to somata of neurons in the PG, certain somata being virtually surrounded by immunoreactive varicosities. Some nerve endings were immunoreactive for either CGRP or GAL; in others, immunoreactivity for CGRP and GAL coexisted. Likewise, many perikarya in DRG were CGRP immunoreactive, fewer were GAL immunoreactive, and in some immunoreactivity for CGRP and GAL coexisted. The results suggest there are subpopulations of neuropeptide-containing sensory nerve endings in the PG; some contain CGRP, some contain GAL, and in some CGRP and GAL coexist. These substances contained in sensory nerve endings could have important roles in pelvic ganglionic functions.

Animals↗
PubMed1983

Kainate resistant neurons in peripheral ganglia of the rat.

Neurons of dorsal root ganglia and of superior cervical ganglia did not display any morphological signs of degeneration when kainic acid (KA) was administered either systemically (20 and 40 mg/kg) or when it was directly injected (1 microgram). The KA doses used were sufficient to result in heavy destruction of hippocampal CA3/CA4 neurons and neurodegeneration of various brain regions after intracerebroventricular or local application, respectively. The resistance of the peripheral neurons to KA is discussed as a consequence of lacking glutamate inputs.

Animals↗
PubMed1985

Developmental changes in gamma-glutamyl transpeptidase activity in nervous tissues with reference to amino acid transmission processes.

In homogenate supernatants of hippocampal formation and cerebellum of the rat, gamma-glutamyl transpeptidase (gamma-GTP) activities increased about 6 times from postnatal day 6 (0.178 +/- 0.02 and 0.187 +/- 0.007 U/g wet wt., respectively) to day 100. In dorsal root ganglia (0.183 +/- 0.003 U/g at day 6) and superior cervical ganglia (0.188 +/- 0.019 U/g at day 6) in which apparently amino acidergic transmission processes do not occur, enzyme activities were seen to go up 4 times and 2.4 times, respectively. Based on protein, enzyme activities in both brain material and dorsal root ganglia showed a similar pattern, whereas the activity increase in superior cervical ganglia was somewhat gradual (1.4 times from day 6 to day 100). Postnatal changes in gamma-GTP activities indicated a functional correlation with the maturation of amino acidergic structures. Kainic acid added to hippocampal extracts (0.45 and 5.0 mM) and, for comparison, to those of kidney (5.0 mM) did not yield any statistically significant effect on gamma-GTP activity.

Amino Acids↗
PubMed1988

Anatomical localization of afferent and postganglionic sympathetic neurons innervating the rat ovary.

The fluorescent retrograde tracer, True blue, was applied directly to either the superior ovarian nerve (SON) or the ovarian plexus nerve (OPN) in the rat. Afferent perikarya were located in lower thoracic-upper lumbar dorsal root ganglia and projected to the ovary via both nerve routes. Postganglionic sympathetic efferent perikarya were located in both prevertebral and thoracolumbar paravertebral ganglia and also utilized both the SON and OPN to reach the ovary. The significance of the dual origin of postganglionic sympathetic neurons innervating the rat ovary is not known.

Afferent Pathways↗
PubMed1990

Suramin-induced changes in sympathetic neurons: correlation between catecholamine fluorescence, tyrosine hydroxylase immunoreactivity and accumulation of pigment bodies.

The effect of suramin, a strong inhibitor of lysosomal enzymes, was studied in rat superior cervical (SCG) and dorsal root ganglia (DRG). Ten days after administration both types of ganglion showed increased intraneuronal pigment fluorescence; in DRG there were also accumulated pigments in Schwann cells. Ultrastructurally the pigment bodies were membranous or lamellar inclusion bodies. In SCG suramin-induced inclusion bodies were associated with decreased catecholamine fluorescence and tyrosine hydroxylase immunoreactivity, suggesting that altered activity of lysosomal enzymes and the formation of lysosomal inclusion bodies may be coupled with decreased synthesis of neurotransmitters in rat sympathetic neurons.

Adrenergic Fibers↗