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PubMed1978

[Histophysiological modifications of the amnion in the rat at the end of gestation].

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes on their surface have also been observed : density of microvilli increases but single cilia disappear. These changes are related to modifications in the exchange processes.

Amnion↗
PubMed2004

[Application of amnion membrane transplantation combine with mitomycin C in the treatment of pterygium].

PURPOSE: To evaluate the effect of combining amniotic membrane transplantation with mitomycin C to prevent the recurrence in primary pterygium surgery. METHOD: Forty eyes were simply excised pterygium and were applied with amniotic membrane transplantation with 0.2 mg/ml mitomycin. RESULT: Following up 6 to 18 months, the rate of recurrence is 3%. CONCLUSION: Applying amniotic membrane transplantation with mitomycin in operation can decrease pterygium recurrence, and no serious complication happens.

Adult↗
PubMed1990

Comparative study of effects of magnesium and taurine on electrical parameters of natural and artificial membranes. VII. Effects on cellular and paracellular ionic transfer through isolated human amnion.

The comparative effects of 2 mM magnesium and taurine on various components of the human transamniotic conductance, Gt, were observed. The use of both microelectrodes and metabolic inhibitors enables 10 components of Gt to be distinguished: six cellular components (Na-K ATPase, Na-H antiport, Na-K-2Cl cotransport and Na, K, Cl channels), one coupling component, and three paracellular components (Na, K, Cl). Mg increased all components of Gt while taurine only increased five of them (Na and K channels, coupling, Na and K paracellular conductance). A potentiometric effect of taurine on Mg2+ modified membrane, obtained on paracellular components, was not measured on cellular components. There was only a vicarious effect between Mg and taurine on the non-enzymatic cellular and paracellular transfer of Na and K.

Amnion↗
PubMed1988

Surface visualisation of tissue interfaces by scanning electron microscopy. Methods for exposure of the basal lamina and associated structures in human amnion.

Tissue interfaces such as basal lamina have been traditionally investigated in transmission electron microscopy by sections cut vertical to the lamina, presenting information restricted to a single ultrathin plane. In order to overcome this limitation, a methodology for surface visualisation of the underside cell membranes of the amniotic epithelium, the upper and lower basal lamina surfaces, and their relationship to the stromal collagen has been devised. This involves alkaline, detergent or enzymatic loosening and/or removal of the epithelial monolayer prior to fixation, followed by dry fracture after critical point drying. In this way we have visualised large areas of all interfaces and the inter-relationships between these elements during the process of stromal collagen production by the amniotic epithelial cells.

Ammonium Hydroxide↗